| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,744,473 | C→T | 100% | A67T (GCT→ACT) | apt ← | Adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,744,473 | 0 | C | T | 100.0% | 192.3 / NA | 61 | A67T (GCT→ACT) | apt | Adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/28); total (33/28) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATA > USA300TCH1516_ALE/1744335‑1744606 | gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTATAAAGCCAATCCCCATTGAGTAAGTTa > 1:282337/1‑141 (MQ=255) tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACa > 2:70897/1‑141 (MQ=255) tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGCTGCAAAGCCAATCCCCATTGAATAAGTTACa > 1:187747/1‑141 (MQ=255) ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGa > 2:292151/1‑141 (MQ=255) tttAATTGCATCTTTGTGTATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACCTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGcc < 2:311144/141‑1 (MQ=255) ttAATTGCATTTTTGTGCATTGTTAAAACATTTGTACCATATCCTAGGTCATACTCATAACGAATGACCTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCa < 1:49065/141‑1 (MQ=255) tAATTTCATTTTTTTGCATTGTTAAAACATTTGTACCATTTTCAAGGTCATACTCATAACGAATGCCTTCACCAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa < 2:703932/141‑1 (MQ=255) tAATTGCATCTTTTGGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa < 2:406445/141‑1 (MQ=255) tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa < 2:260323/141‑1 (MQ=255) tgtgCATTGTTAAAACATTTGTACCATATTCTAGTTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc > 1:182741/1‑141 (MQ=255) cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc < 1:652118/141‑1 (MQ=255) tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAACCAATGATAAAGCAACGCGCTTc > 2:181300/1‑141 (MQ=255) aCATTTGTACCATATTCTAGGTCATACTAATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCAAATGATAAAGCCACGCGCTTCAGGTCCt > 2:630680/1‑141 (MQ=255) ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa < 2:655626/141‑1 (MQ=255) tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTTCAACGa > 1:311200/1‑141 (MQ=255) atTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAACGCCACGCGCTTCAGGTCCTACCACGATACCaa > 2:365292/1‑141 (MQ=255) atTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCTCTTCAGGTCCTACAACGATATCaa > 1:252622/1‑141 (MQ=255) cTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt > 1:298792/1‑141 (MQ=255) aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct < 1:696047/141‑1 (MQ=255) aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATCGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct > 2:495902/1‑141 (MQ=255) ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGTCAGCCAATGATAAAGCCACGCGCTTCTGGCCCTACAACGATATCAAAAtcta > 1:437442/1‑140 (MQ=255) ggTAATACTCATAACGAATGAATCACCGAGGTAATTTCCCTTCTCTTCTTACACAAACACAGCCCTCCCCCCTTCAATACGTAACAGCACATCCAACGAACACGCCACGCGCTCCAGGTCCTACAACGATATCACCAtccc > 2:29406/1‑139 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAACGATATCAACATTTCTg > 1:168762/1‑141 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg < 1:292151/141‑1 (MQ=255) aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc < 2:139531/141‑1 (MQ=255) tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCGATTGAATAAGTTACAGGAGAGCCAATGATAAAGCCACGCGCTTCAGGTGCTAAAAGTATATAAACATGTCTGTCtttt > 2:256569/1‑141 (MQ=255) tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCAAAACCCATTTGAATAAGTTCCAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCACAGTCtttt > 1:297362/1‑141 (MQ=255) cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGCACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg < 2:11163/141‑1 (MQ=255) cATAACGAATGACTTCACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCATCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg > 2:481005/1‑141 (MQ=255) aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAACGATATCAACATCTCTGTCttttcg > 2:184928/1‑139 (MQ=255) gAATGACGTCACGAGATAATTTCCCTCCTTTTCTGACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATAATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAATATCTCTGTCTTTTGCGTAtt < 1:468678/141‑1 (MQ=255) gtgatgtCACGAGGTAATTTCCCTTCTGTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGTACAGCCAATGATAAAGCCACGCGCTGCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt < 2:589271/135‑1 (MQ=255) ctGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt < 1:515991/140‑1 (MQ=255) aTGACTTCACGAGTTAATTTCCCTTCTTTTCTAACACGTGCAAAGCCAATCCCCGTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTTCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt < 1:432491/141‑1 (MQ=255) tGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTa < 2:49084/141‑1 (MQ=255) cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt > 2:526270/1‑141 (MQ=255) cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt > 2:574011/1‑141 (MQ=255) cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt < 2:721753/141‑1 (MQ=255) cGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGCACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 2:285685/141‑1 (MQ=255) aGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTTTTTTGCGTATTCTTCAAttttgtt > 2:62972/1‑138 (MQ=255) ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCt > 1:285800/1‑141 (MQ=255) tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTAACTGt < 1:526270/141‑1 (MQ=255) gaaTTTCCCTTCTCTGCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGGCAGCCAATGATAAAGCCACGCGCTTCATGCCCTACAACGATATCCACATCTCTGGCTTTTGCGTATTCTACAATTTTATCTGt < 2:4169/140‑1 (MQ=255) aTTTCCCTTCTTTGCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACTACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg < 2:204123/141‑1 (MQ=255) cccTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCCTa > 1:248308/1‑141 (MQ=255) ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACCGCCCATGCTAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACACCCCTGGCCTTTGCTTATTCTACAATTTTATCTGTTGCATAGGCctatc > 1:251078/1‑140 (MQ=255) aaCCGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCTTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc < 2:221046/141‑1 (MQ=255) aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTTTTGCATGGCCATAttcttc > 1:349482/1‑141 (MQ=255) aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc < 1:165193/141‑1 (MQ=255) aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc < 1:70897/141‑1 (MQ=255) aCAGGTGCAAAGCGAATCCCCGTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa < 2:650423/141‑1 (MQ=255) aCAGGTGCAAAGCCAATGCCCACGGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa < 2:728964/141‑1 (MQ=255) gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt > 2:442789/1‑141 (MQ=255) gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCGTTTGCATAGCCATATGCTTCACCAt > 2:607498/1‑141 (MQ=255) aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc < 1:495902/141‑1 (MQ=255) aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGAGAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATCCTTCACCATTATcc > 1:552170/1‑141 (MQ=255) aGCCAATCCCCATTGAATAAGTTACAGTACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa < 2:493842/141‑1 (MQ=255) aaTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAGCATTATCCATAAt > 1:356469/1‑141 (MQ=255) tCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAttt > 1:187750/1‑140 (MQ=255) aTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTTTCCATAATTttagta > 1:308228/1‑141 (MQ=255) gAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATATTATCTGTTGCATAGCCATAGGCTGCCCCATTATCCATAATTGTAGTAata > 2:512922/1‑141 (MQ=255) | GTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATA > USA300TCH1516_ALE/1744335‑1744606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |