Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,651	C→T	9.2%	D170D (GAC→GAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,651	0	C	T	9.2%	160.9 / 3.2	65	D170D (GAC→GAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (23/35);  new base T (2/5);  total (25/40)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110517‑110790                                                                                                                                       |                                                                                                                                            tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                       >  2:759245/1‑141 (MQ=255) tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                       >  2:444125/1‑141 (MQ=255)   tCCGGTTAAAATGGTCGATCCATTTTGCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                     >  1:769101/1‑141 (MQ=18)   tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGTCGgagctct                                                                                                                                     >  2:575317/1‑137 (MQ=255)   tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                     >  2:766676/1‑141 (MQ=255)     cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                   <  1:318086/141‑1 (MQ=255)        ttAAAATGGTCGAAAATAAAATCATACCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                                <  2:177719/141‑1 (MQ=255)         tAAAACGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                               <  2:602119/141‑1 (MQ=255)         tAAAACAGTCAATAATAAAACTATTCCAAAGAATAAAATTAAAGATTAACAAATAATAAAAGAAATCTAAAACTTTAAGTTCGTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                               <  2:704983/141‑1 (MQ=17)            aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGACGCAAAAATAAACACAGACATTGAAAAATAGAACCTCTTTGTTCAAGATAGCGACTTGAAAGATTGGAAAAGTTATAAAGACGGAGATATTTCATATaa                                                                                                                            >  2:765191/1‑141 (MQ=14)              tGGTCGATAATAAAATCATTCCAATAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATc                                                                                                                          >  1:494431/1‑141 (MQ=255)                 tcaataataAAAACATTCCAACAAAAGACATTAAAGATAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAAGATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGATATATTATATAATCCag                                                                                                                       <  2:51342/138‑1 (MQ=17)                     taataaAATCATTCCAACAAAAGAAGTAAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCATTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACAGAGATATTTAATATAATCCaaaaaa                                                                                                                   >  1:394735/1‑136 (MQ=17)                             ccATTCCAACAAAAAAAATCAAAAAACAAAAAATAAAAAAAGAAATCGAAAACCTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGtt                                                                                                           <  2:599684/140‑1 (MQ=17)                                    aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                    <  2:147373/141‑1 (MQ=255)                                     acgaaaGAAATGAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                   <  1:247110/138‑1 (MQ=255)                                     acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                   <  2:232181/141‑1 (MQ=255)                                     acaaAAGAAAACAAATATGTAAAAAGAAAAAAAGAGATCGAAAACTTTAAGGTCTGTGTTCAATATGGCGACTTTAAAAATATGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGACATATACGGCt                                                                                                   >  2:184260/1‑141 (MQ=17)                                      caaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                                  >  2:24268/1‑141 (MQ=255)                                       aaaaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaa                                                                                                 <  1:465006/141‑1 (MQ=255)                                         aaGAAATTAAAGAGGAAAAAATAAAAAAACAAATCCAAAACTTTAAATTCTTTGTTCAATATGGCGAATAAAAAAAAAAAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGTGGTGCCGAGTTATTCGGCTAAat                                                                                               <  2:748688/141‑1 (MQ=17)                                                   aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                     <  2:78005/141‑1 (MQ=255)                                                   aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                     <  1:26001/141‑1 (MQ=255)                                                   aGATAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                     <  2:220608/141‑1 (MQ=255)                                                    gATGAAAAAATAAAAAAAGAAATCGAAAAATTAGAGTTCTTTGATAAATAGGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAAACAAAAGGTGCAGAGTTATTCGGCTAAATATAAATTAAca                                                                                    >  2:336073/1‑140 (MQ=17)                                                     aagcaacaaTAAAAAATGCAACCGAAAATATGAAGTTCTTTGTGCAATATGGCGACTGTAAAAATTTGAAAAATTATAAATACAGAGATATTTCAGATAATCCATAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                   <  2:640528/134‑1 (MQ=17)                                                       gAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                 <  1:311789/141‑1 (MQ=255)                                                        aaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGCGGTGCCGAGTTGTTCGGCTAAATATCAATTCACTAatg                                                                                >  2:650898/1‑141 (MQ=255)                                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                             <  1:759245/141‑1 (MQ=255)                                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                             <  1:444125/141‑1 (MQ=255)                                                             aTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGACAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATTGCAATCAACTAATgaggat                                                                           >  2:172766/1‑141 (MQ=14)                                                                  aaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATaa                                                                      <  1:383059/141‑1 (MQ=255)                                                                      gAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                  >  2:758350/1‑141 (MQ=255)                                                                      aaaaTCTAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                  <  1:390983/140‑1 (MQ=255)                                                                              aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                          >  1:140765/1‑141 (MQ=255)                                                                              aaaCTTTAAGTTCTTAGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                          >  1:575525/1‑141 (MQ=255)                                                                                 cTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTAAAAAGACGAAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATGATAATGTAAAACAATTACg                                                       >  1:644359/1‑141 (MQ=255)                                                                                  tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  2:587219/141‑1 (MQ=255)                                                                                  tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  1:701788/141‑1 (MQ=255)                                                                                         tCTGTGTTCAATATGGCGACTTTAAAAATTCACTAGATTTGAAAGACTTGTATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATCATTATAATCTAACCCCATTACGCACAAcat                                               <  2:542759/141‑1 (MQ=9)                                                                                                  aaTATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                      >  1:314700/1‑141 (MQ=255)                                                                                                        gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                >  1:68929/1‑141 (MQ=255)                                                                                                         cGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAg                               <  1:766676/141‑1 (MQ=255)                                                                                                         cGACTTTAAAAATTTGAAAAAGTATAAAGACGTAGATATTTCATATAATCCAGAGGGGCCGAGTTATTCTGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAg                               <  1:738530/141‑1 (MQ=255)                                                                                                            cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  1:732286/141‑1 (MQ=255)                                                                                                             tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                           <  2:633037/141‑1 (MQ=11)                                                                                                               tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                         <  2:394735/141‑1 (MQ=255)                                                                                                                 aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                       >  2:361640/1‑141 (MQ=255)                                                                                                                  aaaaTTGAAAAATAATAAAAACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                      <  2:328481/141‑1 (MQ=255)                                                                                                                  aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                      <  2:769101/141‑1 (MQ=255)                                                                                                                     attaatgaaTTAAAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAAGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                   <  2:48219/134‑1 (MQ=14)                                                                                                                      ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATCTACCGACGAGTAAAGCTCCAAAg                  >  2:191653/1‑141 (MQ=255)                                                                                                                         aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               <  2:212178/141‑1 (MQ=255)                                                                                                                         aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               <  2:597467/141‑1 (MQ=255)                                                                                                                         aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               <  2:42796/141‑1 (MQ=255)                                                                                                                          tgaaTTATAAAGATGGAGCTCTATCATATAATCAAGAGGTGCCGAGGTATTCGGCGAAATTTCAATTAACTAATGAGGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              >  2:534705/3‑141 (MQ=17)                                                                                                                          tgaaTTATAAAGATGGAGATATATCATATAATCAAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAGATATGATATACCGACGAGTAAAGCTACAAAGTTAt              >  2:708309/3‑141 (MQ=14)                                                                                                                          aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTTTTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              <  1:59804/141‑1 (MQ=255)                                                                                                                             aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCAACGAGTAAAGCTCCAAAGGTaaggt           >  2:53968/1‑137 (MQ=255)                                                                                                                              ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt          <  2:289029/141‑1 (MQ=255)                                                                                                                                    aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg    <  1:260646/141‑1 (MQ=255)                                                                                                                                    aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg    <  2:428757/141‑1 (MQ=255)                                                                                                                                    aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg    <  2:33456/141‑1 (MQ=255)                                                                                                                                     gACGGAGATATTTCATATAATCCAGAGGTGCCGAGTAATTCGGCTAAATATCAATTAATTAATGCTGATAAAAAGGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGATCCAAAGTTATTGTTAAAAGGt   >  1:534965/1‑141 (MQ=18)                                                                                                                                      aCGGAGATATTTCATATAATCCAGCGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt  <  2:179290/141‑1 (MQ=255)                                                                                                                                       |                                                                                                                                            TATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110517‑110790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑