| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,455,698 | 2 bp→AT | 100% | coding (1216‑1217/1260 nt) | lyrA → | Lysostaphin resistance protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,455,698 | 0 | C | A | 89.9% | 211.5 / 19.5 | 69 | coding (1216/1260 nt) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base A (35/26); minor base . (5/2); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | USA300TCH1516_ALE | 2,455,699 | 0 | A | T | 100.0% | 207.6 / ‑6.5 | 71 | H406L (CAT→CTT) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (39/28); total (40/31) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACC > USA300TCH1516_ALE/2455568‑2455831 || aGTTAACAAAGCACCACAAAGTGACGATTTAACAAACGATGCAAATGAAGATGAAATAGAGCACTCAATCAATGAGCATGCGTTTGAAGAATAACACAGTCGTTGCGAAAG‑TGTAATTGATGCA‑AAAAAATTATCGataaa < 2:743978/141‑1 (MQ=255) aaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGCCGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCCAAAAAACTga > 2:21789/1‑141 (MQ=255) aGCACCAAAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTACAAAGAAGACAGACGTT‑CATCAGTTGTAATCGATGCA‑AAAAAATTATCGAAAAAACTgaagaa < 1:555262/141‑1 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaagaaa > 1:383499/1‑140 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc > 2:587125/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGAAAGACCGA‑CAGCAGTTGTAATTGATGCAGAAAAA‑TTATCGAAAAAACTGAAGAAc > 1:175028/1‑141 (MQ=255) ccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATGATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAAAAAAAAAAAAAACTGAAGAACAAt < 2:709651/141‑1 (MQ=255) cacaAAGTAACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATc < 2:514938/141‑1 (MQ=255) acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCt < 2:71951/141‑1 (MQ=255) aaaGAGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTc > 1:526628/1‑141 (MQ=255) aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCaa > 1:346906/1‑140 (MQ=255) gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATa > 2:534433/1‑141 (MQ=255) aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAAGTATCGAAAACACTGAAGAACAATCTTCAGATaa > 1:274004/1‑141 (MQ=255) cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa > 1:505645/1‑141 (MQ=255) cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa < 1:65576/141‑1 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa > 1:206970/1‑141 (MQ=255) aCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataata > 2:739394/1‑141 (MQ=255) gATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTAAAGAAAAATCTTCAGATAAAAACAAATAATAAc > 1:262771/1‑141 (MQ=255) gATTCAAAGGAAGATGAAATAGAGCAAGCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc < 2:133120/141‑1 (MQ=255) aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa < 2:639484/141‑1 (MQ=255) tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaa > 2:750263/1‑141 (MQ=255) cAAATGAAGATTAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg < 1:505909/141‑1 (MQ=255) cAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg > 1:99912/1‑141 (MQ=255) cAAAAGAAGATGAAAGAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg < 2:381049/141‑1 (MQ=255) aTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcgc < 1:357924/141‑1 (MQ=255) gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt > 2:576507/1‑141 (MQ=255) gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt > 2:245158/1‑141 (MQ=255) gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt > 2:576138/1‑141 (MQ=255) gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt > 2:576516/1‑141 (MQ=255) aaaagagAAATCAACAAAAGAAACGGCGACCTAACAAAAAGACAGACGTT‑CATTAGTTGTAATTGATGCA‑AAAAAATTTTCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATCACAAAGCGCTTGCTAGTAc < 1:312134/138‑1 (MQ=255) tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGCCAAACTTGATGATCAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct < 2:162554/141‑1 (MQ=255) tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCGAGAAccc > 1:168030/1‑140 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGCTAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct > 1:70573/1‑141 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACctct > 2:215780/1‑141 (MQ=255) agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCGCtt > 2:673295/1‑141 (MQ=255) agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCGTGCTAGTACCTCtt > 1:47117/1‑141 (MQ=255) aagcagtAAAACGACCCGCGACTTATAAAGAAGAAAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa < 2:212116/135‑1 (MQ=255) aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa < 2:646416/141‑1 (MQ=255) cgtaaaaaCAACCGGCGACTTACAAAGAAAACAAACGGT‑CATCATAAATAATTGATGCA‑AAAACAATACCGAAAAGACAGAATCACAACCTTCAGATAAAAACAAGTAATAACAAAGCTCTTGCTAGTACCTCTTaaaaat < 2:274004/137‑2 (MQ=255) cATTAAAAGAACCTGAGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg > 2:138325/1‑141 (MQ=255) tAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAACCTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCACGTGCTAGTACCTCTCAAAAAcata > 2:365039/1‑140 (MQ=255) aaaaGAACCTGCGACTAATAAAGAAAACAGACGTT‑CATCAGTAGAAATAGAAAAA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga < 2:429024/141‑1 (MQ=255) aaaCCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt < 1:158598/140‑1 (MQ=255) ccTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAAAAATAATAACAAAGCGCTAGCACGTACCTCTTAAAAAGAGAATGCTAGc > 2:359595/1‑141 (MQ=255) gCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg < 2:557410/141‑1 (MQ=255) gCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATGGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTCGCAAg > 2:586966/1‑141 (MQ=255) gCGACCTAGAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAGAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAg < 2:538099/141‑1 (MQ=255) cGACTGATAAAGAAGACAGACGTT‑CATCAGTTGTAATGGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTGAAAAAGATCATGCTAGCAAgc < 1:400622/141‑1 (MQ=255) ttaataAAGAAGACAGACGTT‑CATCAGTTGCAATCGATGCAAAAAAAC‑TATCGAAAAAACTGAAGAACAAAAATAAGATAAAAACAAATAATAACAAAGCGCTTTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt < 2:583345/138‑1 (MQ=255) cTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt < 1:321379/141‑1 (MQ=255) ttATAAATAAGACAGACTTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtt < 2:612835/141‑1 (MQ=255) ttATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTACTAGTACCACTTAAAAAGATGATGCTAGAGAGGGCtt > 1:4894/1‑141 (MQ=255) aagaCAGACGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAAGATGCCAGCAAGCGCTTTTctatac < 1:570221/141‑1 (MQ=255) agacagacGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAACATGATGCTAGCAAGCGCTTTTctatact > 1:537871/1‑141 (MQ=255) acagacGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAGGCGCTTTTctatactat > 1:613049/1‑141 (MQ=255) acagacGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat > 1:148551/1‑141 (MQ=255) acagacGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat > 1:368960/1‑141 (MQ=255) acagacGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAAACTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTCAAAAGATGATGCTAGCCAGGGCATTTctatcccat > 1:238008/1‑136 (MQ=255) agacGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAATACCTCTTAAAAAGATGATGCTAGCAAGCCCTTTTCTATACtatat > 2:96330/1‑141 (MQ=255) acGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCCGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATActatctat > 2:520043/1‑141 (MQ=255) cGTT‑CATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt > 1:391133/1‑141 (MQ=255) t‑catcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt < 2:99912/141‑1 (MQ=255) atcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt < 1:476499/141‑1 (MQ=255) atcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt < 2:505645/141‑1 (MQ=255) tcaGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt > 1:265571/1‑141 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTCGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATCCTATATATTAttttt > 2:98962/1‑141 (MQ=255) caGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc > 1:21740/1‑141 (MQ=255) aaTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt > 2:683375/1‑141 (MQ=255) ttGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt < 2:563503/141‑1 (MQ=255) tgcaaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca < 1:252175/137‑1 (MQ=255) caaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATCAATATTTTGTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACAcc < 1:177625/139‑1 (MQ=255) || AGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTT‑CATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACC > USA300TCH1516_ALE/2455568‑2455831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |