New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	102 (0.780)	25 (0.260) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	8/218	6.1	27.0%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
	?	USA300TCH1516_ALE	1426517 =	81 (0.620)					intergenic (‑248/+724)	pstS/cvfB	Phosphate‑binding protein PstS/Conserved virulence factor B
Rejected: Coverage evenness skew score above cutoff.

TACATAAAAAATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTC  >  USA300TCH1516_ALE/1426370‑1426662                                                                                                                                                    |                                                                                                                                                  tACATAAAAAATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTaaaaaa                                                                                                                                                   <  2:809266/148‑1 (MQ=255) tACATAAAAAATTGTTAAGAAAATGTAAATATTAAATGTAAGTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTaaaaaa                                                                                                                                                   <  1:1314970/148‑1 (MQ=255) aactgaaaaaaTTGTTAAAAAAATGTAAAGATTAATTTTTGTTAATCTAAAAAATATTGATTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGAACATAAATCCCTaaaaaa                                                                                                                                                   <  2:670208/143‑1 (MQ=255)      aaaaaaTTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagc                                                                                                                                              <  1:831878/148‑1 (MQ=255)      aaaaaaTTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagc                                                                                                                                              >  1:479153/1‑148 (MQ=255)        aaaaTTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagcag                                                                                                                                            <  2:741178/148‑1 (MQ=255)          aaTTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATAAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTa                                                                                                                                          <  1:545507/148‑1 (MQ=255)             tGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGa                                                                                                                                       <  2:1120084/148‑1 (MQ=255)              ttaaaGAAAA‑GTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATa                                                                                                                                     >  2:585857/4‑148 (MQ=255)                tAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATaa                                                                                                                                    >  1:1097788/1‑148 (MQ=255)                   gAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAttt                                                                                                                                 >  1:188576/1‑148 (MQ=255)                   gAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAAAGCAGTAAGATAAttt                                                                                                                                 >  2:497605/1‑148 (MQ=255)                    aaaaTGTACATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAtttt                                                                                                                                <  1:1128260/148‑1 (MQ=255)                    aaaaTGTAAATATTAATTTTTGTTAATCTTACAAATATTGCTTCATTAGTAAAATTAAACTGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAtttt                                                                                                                                >  2:1000982/1‑148 (MQ=255)                    aaaaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAtttt                                                                                                                                <  1:1201534/148‑1 (MQ=255)                    aaaaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAtttt                                                                                                                                >  2:537059/1‑148 (MQ=255)                      aaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCa                                                                                                                              <  2:1340924/148‑1 (MQ=255)                        tGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAAt                                                                                                                            <  1:451283/148‑1 (MQ=255)                        tGTAAATATTAATTTTTGTTAATCAAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAAt                                                                                                                            <  2:832840/148‑1 (MQ=255)                           aaaTATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAg                                                                                                                         <  2:31164/148‑1 (MQ=255)                              tatTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGaaa                                                                                                                      <  2:521329/148‑1 (MQ=255)                              gatgaaTTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGaaa                                                                                                                      <  2:1410127/144‑1 (MQ=255)                                ttAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAat                                                                                                                    <  1:1203847/148‑1 (MQ=255)                                    tttttGTTAATATAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCt                                                                                                                <  1:29136/148‑1 (MQ=255)                                       ttGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGTACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTAc                                                                                                             <  2:619705/148‑1 (MQ=255)                                           tAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTActgct                                                                                                         >  2:167000/1‑148 (MQ=255)                                           tAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATAACTTActgct                                                                                                         >  2:543592/1‑148 (MQ=255)                                                 aaaaaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTtctc                                                                                                   <  1:1152075/148‑1 (MQ=255)                                                 aaaaaaTATTGCCTCCTTAGTAAACTTAAAATGCTTACTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGCTAATTTTCAATTAGAAAATATCTTACTGCTGTtctc                                                                                                   <  1:1250121/148‑1 (MQ=25)                                                   aaaaaaTTGTCTAATTATTAAAATTAAAATGATAAATAAAAAATCATCGAAATTTAAAAATAAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTa                                                                                                 <  2:313470/148‑1 (MQ=18)                                                   aaaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCGTACTGCTGTTCTCTa                                                                                                 <  2:567047/148‑1 (MQ=255)                                                   aaaaTATTGCTTCAGTAGTAAAATTAAAATGCTTCCTGAAAAATCAGCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTa                                                                                                 <  2:184564/148‑1 (MQ=255)                                                    aaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTAt                                                                                                <  2:1056766/148‑1 (MQ=255)                                                     aaTATTGCTTCATAAGTAAAATTAAAATGCTTCCTGAAAACACATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATCAACCCATCAAAAACCATCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTAtt                                                                                               <  2:924992/148‑1 (MQ=25)                                                                aTTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACt                                                                                    >  1:812426/1‑148 (MQ=255)                                                                aTTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACt                                                                                    >  1:812113/1‑148 (MQ=255)                                                                   aGTAAAAATAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACAAAAAAAAATAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCg                                                                                 <  2:1088722/148‑1 (MQ=21)                                                                    gTAAAATTAAAATGGTTGATGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCGGTTCTCTATTTATACAATACTTCGt                                                                                >  1:1034354/1‑148 (MQ=255)                                                                     taaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATCCAATACTTCGTa                                                                               >  1:1125916/1‑148 (MQ=255)                                                                     taaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTa                                                                               >  2:682953/1‑148 (MQ=255)                                                                      aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTAt                                                                              >  2:289779/1‑148 (MQ=255)                                                                      aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTAt                                                                              <  1:1169660/148‑1 (MQ=255)                                                                         attaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAAATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGa                                                                           >  1:463770/1‑148 (MQ=255)                                                                          ttaaaatCCTTCCTGAAAAATCACCGAAATTAAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGaa                                                                          <  1:447334/148‑1 (MQ=255)                                                                          ataaaatGCATACTGAAAAATCATCGAAAGTTAAATATGAATAAAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGaa                                                                          <  2:426417/147‑1 (MQ=255)                                                                              aatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTTGTATTGAATGGc                                                                      <  1:959905/148‑1 (MQ=255)                                                                                  cTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCg                                                                  >  2:28680/1‑148 (MQ=255)                                                                                       ggAAAAATCTTCGAAATTGAAAAAAGAAAACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTg                                                             <  2:1081026/147‑1 (MQ=14)                                                                                                  cGAAATATAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTca                                                  <  2:807506/148‑1 (MQ=255)                                                                                                   gAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTcac                                                 <  1:1303498/148‑1 (MQ=255)                                                                                                             tatGAATACAAAATTGGCTGAAACATAAATCCCTAAAACACCTACAGTAAGATCATTTTTATACGTAAAAAATCTAACTACTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTaa                                       >  2:641566/1‑148 (MQ=17)                                                                                                             tatGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCCCATTAATTTaa                                       >  2:1327249/1‑148 (MQ=255)                                                                                                             tatGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTATTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTaa                                       >  2:151799/1‑148 (MQ=255)                                                                                                                gAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACGGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAAt                                    <  2:1079442/148‑1 (MQ=255)                                                                                                                  aTAAAAAGATGGCTGAGACATAAATCCCTAAAAAAACAGAAGTAAGATAATTGTCAATTAGAAAATATCTTACTGCTGTTCTCTCTGTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTCCTGTAAAATTc                                  <  2:205227/148‑1 (MQ=17)                                                                                                                   tccAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCt                                 <  2:411984/146‑1 (MQ=255)                                                                                                                   tACAAAAGTGGCTGGGACCTAATTCCCTAAAATAACAGCAGTAAGATAATTTTCCATTAGAAAATATCCTACTGCTGTGCTCGCTTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCt                                 <  1:178050/148‑1 (MQ=17)                                                                                                                   tACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCt                                 <  1:608901/148‑1 (MQ=255)                                                                                                                   tACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCt                                 <  1:1007016/148‑1 (MQ=255)                                                                                                                   tACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCATCTGTCACATTACTGTAAAATTCt                                 <  1:698009/148‑1 (MQ=255)                                                                                                                   aaCAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCt                                 <  2:923518/147‑1 (MQ=255)                                                                                                                    aCAAAAATGGCTGGAACAAAAAACCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTCAAACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCta                                <  2:1209889/148‑1 (MQ=17)                                                                                                                      aaaaaTGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCtata                              >  2:972835/1‑148 (MQ=255)                                                                                                                      aaaaaTGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCtata                              >  2:706173/1‑148 (MQ=255)                                                                                                                      aaaaaTGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCtata                              >  1:353658/1‑148 (MQ=255)                                                                                                                      aaaaaTGGCTGGGACATAAATCACTAAAAAAACAGCAGTAAGATAATTTTAAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTTTAAAATTCtgta                              >  2:737492/1‑148 (MQ=255)                                                                                                                       aaaaTGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATaa                             >  2:484712/1‑148 (MQ=255)                                                                                                                                gggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt                    <  1:1324148/148‑1 (MQ=255)                                                                                                                                gggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt                    <  1:913202/148‑1 (MQ=255)                                                                                                                                gggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt                    <  1:1272772/148‑1 (MQ=255)                                                                                                                                  gACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttttt                  <  2:479153/148‑1 (MQ=255)                                                                                                                                      tAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTTCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtgatg              >  1:363338/1‑148 (MQ=255)                                                                                                                                      tAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACAGTACTGTAAAATTCTATCATTAGAATTTTtgatg              >  2:1394269/1‑148 (MQ=255)                                                                                                                                        aaTCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGAc            >  1:1125582/1‑148 (MQ=255)                                                                                                                                                 aaaaaCAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACACTAATTCTTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTTAAATTCTATAAATAGTATTTTTGATGACTGGTACCtt   >  1:1172679/1‑148 (MQ=11)                                                                                                                                                 aaaaaCAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCtt   >  1:413432/1‑148 (MQ=39)                                                                                                                                                 aaaaaCAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCtt   >  2:744765/1‑148 (MQ=38)                                                                                                                                                 aaaaaCAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCtt   >  2:1317141/1‑148 (MQ=39)                                                                                                                                                 aaaaaCAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCtt   >  2:1049580/1‑148 (MQ=39)                                                                                                                                                 aaaaaCAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCtt   >  2:1057327/1‑148 (MQ=38)                                                                                                                                                  aaaaCAGCATGAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTc  <  2:264164/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TACATAAAAAATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTC  >  USA300TCH1516_ALE/1426370‑1426662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.