New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	102 (0.780)	25 (0.260) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	8/218	6.1	25.4%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
	?	USA300TCH1516_ALE	= 2072043	96 (0.740)					intergenic (+538/+35)	USA300HOU_RS10445/USA300HOU_RS10450	hypothetical protein/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.

AATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTACACCAACACCAGCAACTA  >  USA300TCH1516_ALE/2071896‑2072190                                                                                                                                                    |                                                                                                                                                     aaTGGCGAGACTCCTGAGGGAGTAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtt                                                                                                                                                      <  2:27603/148‑1 (MQ=17) aaTGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTCTAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtt                                                                                                                                                      <  2:913053/148‑1 (MQ=17)  aTGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGttt                                                                                                                                                     <  1:844424/148‑1 (MQ=32)  aTGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGttt                                                                                                                                                     <  1:767516/148‑1 (MQ=32)   tGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATTCGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtttt                                                                                                                                                    <  2:1042799/148‑1 (MQ=17)   tGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtttt                                                                                                                                                    >  1:157760/1‑148 (MQ=32)   tGGCGAGACCCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtttt                                                                                                                                                    >  1:1407216/1‑148 (MQ=17)    ggCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGttttt                                                                                                                                                   >  2:1135218/1‑148 (MQ=32)    ggCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGttttt                                                                                                                                                   <  1:813787/148‑1 (MQ=32)     gCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtttttt                                                                                                                                                  <  2:20621/148‑1 (MQ=32)     gCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtttttt                                                                                                                                                  >  2:1026363/1‑148 (MQ=32)     gCGAGACTCCGGAGGGAGCAGTGCAAGTCGAAGCCCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGtttttt                                                                                                                                                  <  2:877861/148‑1 (MQ=12)          aCTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGa                                                                                                                                             <  1:424340/148‑1 (MQ=32)             ccTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGAttt                                                                                                                                          <  1:911677/148‑1 (MQ=32)              cTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTa                                                                                                                                         >  1:1022021/1‑148 (MQ=32)                  gggAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTc                                                                                                                                     >  1:764150/1‑148 (MQ=32)                  gggAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAAGACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTc                                                                                                                                     >  1:940744/1‑148 (MQ=17)                    gAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTccc                                                                                                                                   >  2:1156557/1‑148 (MQ=32)                       cAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCacc                                                                                                                                >  2:239759/1‑146 (MQ=17)                       cAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGc                                                                                                                                >  2:544646/1‑148 (MQ=32)                        atgaccAGTCGAAGACCAAGGCTGAGACGGCACCCTAGAAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACATCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGcc                                                                                                                               <  2:914461/144‑1 (MQ=12)                        aGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGcc                                                                                                                               <  2:440561/148‑1 (MQ=32)                        aGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGcc                                                                                                                               <  1:1256032/148‑1 (MQ=32)                        aGTGCCAGCCGAAGACCAAGGCTGAGACGGCATCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGcc                                                                                                                               <  2:947872/148‑1 (MQ=17)                         gTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCct                                                                                                                              <  2:409008/148‑1 (MQ=32)                         gTGCCAGGCGAAGACCAAGGCTGAGACGGAACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCct                                                                                                                              <  2:862317/148‑1 (MQ=17)                          tGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCCTTCACCAAGAGGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCctc                                                                                                                             <  1:668883/148‑1 (MQ=11)                          tGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAATATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTACGTCCCAGCctc                                                                                                                             <  1:121778/148‑1 (MQ=17)                          tGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCctc                                                                                                                             <  2:79727/148‑1 (MQ=32)                          tGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCctc                                                                                                                             <  1:1403465/148‑1 (MQ=32)                          tGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCctc                                                                                                                             <  1:1155243/148‑1 (MQ=32)                           gCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCctct                                                                                                                            <  2:316559/148‑1 (MQ=32)                            ccAGTCGAAGACCAAGGCTGAGACGGCAACCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTGAGGGATTTATGTCCCAGCCTCTa                                                                                                                           <  2:142437/148‑1 (MQ=17)                                tCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAGGTATTGTATAAATAGAGACCAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAACTg                                                                                                                       >  1:887606/1‑148 (MQ=16)                                tCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATg                                                                                                                       <  1:638818/148‑1 (MQ=35)                                      aCCAAGGCTGCGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGttatta                                                                                                                 <  2:563608/148‑1 (MQ=255)                                       ccAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAg                                                                                                                >  2:730579/1‑148 (MQ=255)                                            gCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTGGTTC‑tt                                                                                                          >  1:173885/1‑148 (MQ=255)                                                  aCGGCACCCTAGGAAAGCGAAGCCATTCAATCCGAAGTAATGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAACTATCTTACTGCTGGTTTTTAGGGATGTATGACCCAGCCTCTAAATATTATTAGGTA‑TTTTTACt                                                                                                    >  2:1105200/1‑148 (MQ=14)                                                  aCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACt                                                                                                    <  2:1022021/148‑1 (MQ=255)                                                    ggCAACCTAGGAAAGCAAAGCCATTAAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACttt                                                                                                  <  2:386546/148‑1 (MQ=255)                                                          cTAGGAAAGCGAACCCATTCAATAGGAAGTATTGTAGAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAatat                                                                                            <  1:132442/148‑1 (MQ=255)                                                           tAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATc                                                                                           <  1:413764/148‑1 (MQ=255)                                                             ggAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGGCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAg                                                                                         >  2:1377487/1‑148 (MQ=255)                                                             ggAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTCAATGTTATTAGTTA‑TTTTTATTTTCAATATCAg                                                                                         >  1:1048047/1‑148 (MQ=255)                                                                aaGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGaaa                                                                                      >  1:1267361/1‑148 (MQ=255)                                                                 aGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGTGATTTATGTCCCAGCCTCTAAATGTTGTTAGTTA‑TTTTTACTTTCAATATCAGAaat                                                                                     >  1:354680/1‑148 (MQ=255)                                                                  gCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAaata                                                                                    >  1:575254/1‑148 (MQ=255)                                                                   cGAAGCCATTCAATACGAAGTTTTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAaataa                                                                                   <  1:702608/148‑1 (MQ=255)                                                                    gAAGCCATTCAATACGAAGTATAGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATATATGTCCCAGCCTCTAAATGCTATTAGTTA‑TTTTTACTTTCAATATCAGAaataat                                                                                  >  2:12648/1‑148 (MQ=255)                                                                      aGCCATTCAATTCGAAGTATTGTATGAATAGAGAACGGCTGTAAGATATTTTCTAATTGAAAATTATCTTACTGTTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAAttt                                                                                <  2:806117/148‑1 (MQ=18)                                                                         cATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTAGCTTTCTGCTTTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCtt                                                                             >  2:641978/1‑148 (MQ=255)                                                                                  cGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTCCTCTCAAGATCAGAAATAATTTCTTTAGTTtttc                                                                    <  2:1000816/148‑1 (MQ=255)                                                                                  cGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttc                                                                    <  2:1332094/148‑1 (MQ=255)                                                                                   taaGTATTGTATAAATAGATAACAGCATTAATATATTTTCTAATTTAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttct                                                                   <  2:1169223/147‑1 (MQ=255)                                                                                    aaGTATTGTATAAATAGAGAACAGGAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttctt                                                                  <  1:848890/148‑1 (MQ=255)                                                                                    aaGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttctt                                                                  <  2:734592/148‑1 (MQ=255)                                                                                     aGTATTGTATAAATCGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttcttt                                                                 <  2:727646/148‑1 (MQ=255)                                                                                     aGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTCTTAGTTA‑TTTTTACTTTCAATATTAGAAATAAATTCTTTAGTTtttcttt                                                                 >  1:27329/1‑148 (MQ=255)                                                                                          tGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAata                                                            >  1:377306/1‑148 (MQ=255)                                                                                             ataAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAatattt                                                         >  2:1376023/1‑146 (MQ=255)                                                                                              taAATTGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCtt                                                        <  1:452415/148‑1 (MQ=255)                                                                                              taAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCCGAAATAATTTCTTTAGTTTTTCTTTCAATATCtt                                                        <  2:782358/148‑1 (MQ=255)                                                                                                aaTAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAatttatcct                                                      >  2:691806/1‑141 (MQ=255)                                                                                                 aTAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTGTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCaa                                                     >  2:85827/1‑148 (MQ=255)                                                                                                   agagAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCaaaa                                                   <  1:1358946/148‑1 (MQ=255)                                                                                                    gagaACAGCAGTAAGATATTTTCTAATTGAAACTTATCTTCCTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTACTTGTTGCCGTC‑ATATCCGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAAt                                                  >  1:1093436/1‑148 (MQ=21)                                                                                                    gagaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAAt                                                  <  2:193050/148‑1 (MQ=255)                                                                                                    gagaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAAt                                                  >  1:968920/1‑148 (MQ=255)                                                                                                    aagaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAAt                                                  <  1:520774/147‑1 (MQ=255)                                                                                                     agaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGTTTTATGTATCAGCCTCAAAATGAAATTATTTA‑TTTTTTGTTTCATTATATGAAATGATTTCTTTAGTATTTCTTTCAATATCTTCAACATc                                                 >  2:521020/1‑148 (MQ=18)                                                                                                     agaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATc                                                 >  1:1303788/1‑148 (MQ=255)                                                                                                     agaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATc                                                 >  2:76254/1‑148 (MQ=255)                                                                                                        aCAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCttt                                              >  1:1044248/1‑148 (MQ=255)                                                                                                         ctgcagTAAGATATTTTCTAATTGAAAAGTATCTTACTGCTGTTTTTTAGGGATTCATGTACCAGCCTCTAATTGTTATTAGTTG‑ATTTTACTTTCAATATCTGATATAATTTAGTTAGTTTTTCTTTCAATAGCTTCAAATACtttt                                             >  2:384602/3‑148 (MQ=21)                                                                                                         cagcagTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCtttt                                             >  1:182621/1‑148 (MQ=255)                                                                                                         cagcagTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCtttt                                             >  1:778644/1‑148 (MQ=255)                                                                                                         cagcagTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTAAGTCCAAGACTGTAAATGTAATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTATTTCAATATCTTCAAAATAtttt                                             >  2:1210990/1‑148 (MQ=25)                                                                                                            ccgTAAGTTATTTTCTACTTGATTTTTATTTTATTGTTGGTTTTTGGTGATTTTTGTCTTAGTCTCTAAATGTTTTTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATATTTTTTa                                          >  2:1180149/3‑148 (MQ=255)                                                                                                            cagTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATTTTCAAAATCTTTTTTa                                          >  1:768248/1‑148 (MQ=255)                                                                                                            cagTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTACAATATCCTCAAAATCTTTTTTa                                          >  1:661729/1‑148 (MQ=255)                                                                                                              gTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGTCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTaaa                                        <  2:222725/148‑1 (MQ=255)                                                                                                                 aGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGat                                     <  2:1087812/148‑1 (MQ=255)                                                                                                                   atatTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAGTGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGatat                                   >  1:1258887/1‑148 (MQ=255)                                                                                                                   atatTTTCGAATTGAAAATTATCTTACTGCTGTGTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGatat                                   <  2:656717/148‑1 (MQ=255)                                                                                                                   actttttCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGTATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGatat                                   <  2:743259/145‑1 (MQ=255)                                                                                                                               tGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGcta                       <  2:178526/148‑1 (MQ=255)                                                                                                                               tGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGcta                       <  1:360371/148‑1 (MQ=255)                                                                                                                               tGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGcta                       >  1:234616/1‑148 (MQ=255)                                                                                                                                  aaaTTATCTTACTGCTGTTTTTTATGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGctacta                    >  1:1049870/1‑148 (MQ=255)                                                                                                                                  aaaTTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGctacta                    <  2:208554/148‑1 (MQ=255)                                                                                                                                     ttATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTTATAATTTTAGCACATGCTACTacac                 >  2:1014169/1‑148 (MQ=38)                                                                                                                                           tACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTacaccaacac           >  2:142680/1‑148 (MQ=255)                                                                                                                                              tgctgTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACAACACCAACACCAg        >  1:841438/1‑148 (MQ=255)                                                                                                                                              tgctgTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACAACACCAACACCAg        >  1:716679/1‑148 (MQ=255)                                                                                                                                              tgctgTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACAACACCAACACCAg        >  1:143371/1‑148 (MQ=255)                                                                                                                                                ctgTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTACACCAACACCAGCa      <  1:1156557/148‑1 (MQ=255)                                                                                                                                                    tttttAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTACACCAACACCAGCAACTa  <  1:1135218/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     AATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTA‑TTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTACACCAACACCAGCAACTA  >  USA300TCH1516_ALE/2071896‑2072190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.