Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,435,0640GC20.6% 264.2 / 20.7 107A141P (GCT→CCT) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (36/49);  new base C (22/0);  total (58/49)
Fisher's exact test for biased strand distribution p-value = 2.05e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 109 total aligned reads displayed.

AATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAA  >  USA300TCH1516_ALE/1434917‑1435209
                                                                                                                                                   |                                                                                                                                                 
aaTGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGTTATGAATGCTACTCTCGGTGGTCGTg                                                                                                                                                   >  1:755301/1‑148 (MQ=255)
aaTGCACGAATCGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTg                                                                                                                                                   <  1:244313/148‑1 (MQ=255)
      cGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGGTACaa                                                                                                                                             >  2:1162886/1‑148 (MQ=255)
      cGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACaa                                                                                                                                             >  1:268185/1‑148 (MQ=255)
        aaTTGAACGAGGTGCCTTTATTAGAGAACAAGACATTATCGAAGATGGTTCTGTGGTTATGATGGGCGCAAAGATGAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACt                                                                                                                                           <  2:250464/148‑1 (MQ=255)
               ccAGGAGCGTTTATTAGAGAAAAAGACATTATTGAAGATGGTGCTGTCGTTATTATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTaaaa                                                                                                                                    <  2:480357/148‑1 (MQ=255)
                   gTGCGTTTATTAGAGAACCAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGt                                                                                                                                <  1:1071505/148‑1 (MQ=255)
                    tGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTa                                                                                                                               <  1:582662/148‑1 (MQ=255)
                      cGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACa                                                                                                                             <  1:1142718/148‑1 (MQ=255)
                       gTTTATTAGATAACAAGCCATTATTGAAGATGGTGCTGGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACAt                                                                                                                            <  1:1196064/148‑1 (MQ=255)
                            ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCACTAGAAAAAAAGAACACGTAgg                                                                                                                       >  2:646413/1‑148 (MQ=255)
                            ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGCTATAACTAGAAAAAAGATACATgcaca                                                                                                                       >  2:646412/1‑144 (MQ=255)
                             taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTCGTCGTGCTACAACTGGTAAAAATGTACATGTAggg                                                                                                                      >  1:781808/1‑148 (MQ=255)
                             taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTGCAACTGGTAAAAATGTACATGTAggg                                                                                                                      >  1:1335402/1‑148 (MQ=255)
                              agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                     >  1:1216444/1‑148 (MQ=255)
                              agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTATCATTAGAAAAAAAGTACATGTAgggg                                                                                                                     >  2:694331/1‑148 (MQ=255)
                              agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTCCAATTAAAAAAAAAATACATTTAgggg                                                                                                                     >  2:1200304/1‑148 (MQ=255)
                               gagaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAAGGTACATGTAGGGGc                                                                                                                    >  1:1235550/1‑148 (MQ=255)
                                  aaaaaGCCATTATTGAAGATGGTGCTGTCGTTTTGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACACTGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                 <  2:786073/145‑1 (MQ=255)
                                  aaCGAGCCATTATTTAAGATGGTGCTTGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                 <  2:803735/148‑1 (MQ=255)
                                     aaGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACAACTAAAAAAAAAATACATGAAGGGGCTGgcgc                                                                                                              >  2:1149/1‑148 (MQ=255)
                                       gcaatttttGAAGATGGTGGTGTCGTTAGGATGGGCGCAACAATTAATATTGGGGCAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                            <  1:37915/145‑1 (MQ=255)
                                       gCCATTATTGAAGATGGTGCTGTCGTTATGAGGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                            <  1:582414/148‑1 (MQ=255)
                                       gCCATTAGTGAATAAGGTGGTGTCGTTATGAAGGGCGCAACAATTAATATTGGCACAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                            <  2:135826/148‑1 (MQ=255)
                                         caattatGAAGATGGGGCTATCGGTATGATGGGCGTAACAATTAATGTTGGCGCAGTCGTTGTCGAAGGTACAATGATGGATATGAATGCTACTCGCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                          <  2:707239/142‑1 (MQ=255)
                                         cATAATAGAAGATGGTGCTGTCGTAATGCAGGGCGCAACAATGGATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGACTGCTTCTGTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                          <  2:1285435/148‑1 (MQ=255)
                                          attattGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGTAGTACCGATGAAAGGTAACGTGCATTACACGAAGGCTACTCTCGTTGATCGTGCTACGACTGATAGAAATGTACATGTGGGGGCTGGCGCAGTAt                                                                                                         >  1:1392300/1‑148 (MQ=255)
                                             attTAAGATGGTTCTAGCGGTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                      <  2:1184297/148‑1 (MQ=255)
                                              ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTCGGGGCTGGCGCAGTATTAGc                                                                                                     >  1:179423/1‑148 (MQ=255)
                                              ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                     >  1:1164182/1‑148 (MQ=255)
                                              ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                     >  1:806476/1‑148 (MQ=255)
                                               tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                    >  1:1167532/1‑148 (MQ=255)
                                               tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                    >  1:420433/1‑148 (MQ=255)
                                               tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                    >  1:1329508/1‑148 (MQ=255)
                                                gAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTGAAAAAAATGTACATGTAGGGGCTGGCACAGTATTAGCAg                                                                                                   >  2:169231/1‑148 (MQ=255)
                                                 aaGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAGTAAAAAAAAAATACATGTAGGGGCTGGCGAAGTAAGAGCacg                                                                                                  >  2:1227963/1‑146 (MQ=255)
                                                  aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACACGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                 >  1:661539/1‑148 (MQ=255)
                                                  aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                 >  2:655407/1‑148 (MQ=255)
                                                  aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAggg                                                                                                 >  2:894240/1‑147 (MQ=255)
                                                  aGATGGGGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATAATTGATATGAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                 <  2:1412530/148‑1 (MQ=255)
                                                     tGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGtgtg                                                                                              <  1:1174347/148‑1 (MQ=255)
                                                       gtccTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATTAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCATTATTAGCAGGTGTGAt                                                                                            <  1:695697/145‑1 (MQ=255)
                                                       gTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGAt                                                                                            <  1:89040/148‑1 (MQ=255)
                                                         gCTGTCGTTATGATGGGCGCAACAATTAATATGGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGGATTAGCAGGTGTGAGTg                                                                                          >  1:146675/1‑148 (MQ=255)
                                                            gTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAc                                                                                       >  1:549060/1‑148 (MQ=255)
                                                              cGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAccc                                                                                     >  1:600991/1‑148 (MQ=255)
                                                               gTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGTTATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                    <  2:755301/148‑1 (MQ=255)
                                                               gTTATGATGGGCGCAACAATTAACATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                    <  1:55719/148‑1 (MQ=255)
                                                               gTTATAAGGGGCGTAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                    <  2:840938/148‑1 (MQ=255)
                                                                    gatgGGCGCAACAATTAATATTGGCGCAGTCGTTGGAGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGt                                                                               >  1:894965/1‑148 (MQ=255)
                                                                          cgcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAAAACATGTAGGGGCCGGCGCAGTACTAGCAAGATTGATTGAACCCCCTACTGcagca                                                                         >  2:1087074/1‑144 (MQ=255)
                                                                           gaaacaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                        <  1:591901/146‑1 (MQ=255)
                                                                           acaacaaATAAAACTAGCGCAGTCGTTAGCAAAGAAACAACGATTAATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                        <  1:375753/147‑1 (MQ=255)
                                                                              acaaTTAATATTGGCGCAGTCGTTAGCGGAGGTATAATGATTGATATAAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCgg                                                                     <  1:533733/148‑1 (MQ=255)
                                                                               caaTTAATATTGGTGCGGTGGTTGGCGAAGGTACAAGGATTGATATGAATGCTGCCCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGTTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGt                                                                    <  1:46820/148‑1 (MQ=255)
                                                                                caTTAATATGTGCCAAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTCGCAGGCGCGATTGCACCCCCTAGTGCTTCACCGGtt                                                                   <  2:1398803/147‑1 (MQ=255)
                                                                                aaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCTGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                   <  1:1331992/148‑1 (MQ=255)
                                                                                aaTTAATATGGGCGCAGTCGTTGGCGAGGGTGCAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                   <  2:220957/148‑1 (MQ=255)
                                                                                     atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                              <  1:156017/148‑1 (MQ=255)
                                                                                     atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCCTATCACTAATAAAAATATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                              >  2:418411/1‑148 (MQ=255)
                                                                                         tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTACCATTAAAAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAg                                                          >  2:1245472/1‑148 (MQ=255)
                                                                                           gcgcAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGAATTATCAGGTGGGATTGAAGCCCCTAGTGCTTCAACGGTTATAAACGAGga                                                        >  2:211889/1‑148 (MQ=255)
                                                                                               aGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTCTAATCGAGgatgat                                                    >  1:113084/1‑148 (MQ=255)
                                                                                               aGTCGTTGGCGAAGGTACAATGATTGAAATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGgatgat                                                    <  1:979172/148‑1 (MQ=255)
                                                                                                  cGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGAGGATGTa                                                 >  1:766570/1‑148 (MQ=255)
                                                                                                     tGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAGCGAGGATGATGTATTa                                              <  2:1005977/148‑1 (MQ=255)
                                                                                                       gCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAAGAGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTaaa                                            >  2:254310/1‑147 (MQ=255)
                                                                                                        cGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCGGTATTAGCAGGTGTGATTGAACCCCCTAGAGCTGCACCGGTTATAAACGAGGATGATGTATTAATc                                           >  2:187177/1‑148 (MQ=255)
                                                                                                         gAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGTTGTGATTGAACCCCCTAGTGCTTCCCCGGTTATACTCGAGGATGATGTATTAATCg                                          >  2:1012947/1‑148 (MQ=255)
                                                                                                         gAAGGTACAATGATTGATATGAATGCTACTCTCAGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTAATCg                                          >  1:703971/1‑148 (MQ=255)
                                                                                                          aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGAAGTATTAATCgg                                         >  1:593190/1‑148 (MQ=255)
                                                                                                           aGGTAAAATGATTGAGAGGAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGt                                        <  2:456353/148‑1 (MQ=255)
                                                                                                              tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTGAAAAAAAAATACATATAGGGGCTGGAGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCTGTTATAATAGAGAACGATGGATTAATCgggtca                                     >  2:1111803/1‑144 (MQ=255)
                                                                                                              tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAAAACTAGGGGCACGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATCTATGAATCCGTGCa                                     >  2:1146062/1‑148 (MQ=255)
                                                                                                               aaaatgaatgATATAAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCaa                                    <  2:1039641/146‑1 (MQ=255)
                                                                                                                cAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGTACATGTAGGGGCGGGCGCAGTATTAGCAGGTGTGATTGAACCCGCTAGTGCTAAAGCGATAATAATCGAGGATGATGTATTAATCGGTGCaac                                   >  2:1053515/1‑147 (MQ=255)
                                                                                                                    gattaacATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                               <  1:599358/148‑1 (MQ=255)
                                                                                                                    gaatgaaATAAATGCAACACGCGGTGGTCGGGCTACAACTGGTAAAAATGTACATGAAGGGGCTCGCTCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                               <  2:670693/145‑1 (MQ=255)
                                                                                                                      ttgatATGAATGCTCCTCTCGGTGGTCGTGCTACAACTCGTAAAAATGTACATGTAGAGACTGTCGCATTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGt                             <  1:1386660/148‑1 (MQ=255)
                                                                                                                           atGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                        <  1:1149/148‑1 (MQ=255)
                                                                                                                           atGAAGGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                        <  1:981270/148‑1 (MQ=255)
                                                                                                                            tGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAtttt                       >  1:1413360/1‑148 (MQ=255)
                                                                                                                             gAATGCTACTCTCGGTGGTCGTCGTATCATTGAAAAAAAAGTACATGTAGGGACTGGCGCAGCATTCGAAGGTGTGCTTGAACCCCCTAATGGTTGCACGGTTGTAATGGAGGATGATGTATTAATCGTTGCAAATGAAGATATTTTa                      >  2:616418/1‑148 (MQ=255)
                                                                                                                             gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCACTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCGAATGCAGTTATTTTa                      >  2:937542/1‑148 (MQ=255)
                                                                                                                             gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGAAAGTGAGATTGAACCCCCTATTGCTTCTCCGGTTATAATCGAGGGTGATGTCTTATTAGGTGCAAATGGAGTTAGTTTa                      >  2:937691/1‑148 (MQ=255)
                                                                                                                              aaTGTTACTCTCGGTGGTCGTGCTACAACTTGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGGACCGCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTGtttggg                     <  2:870104/148‑4 (MQ=255)
                                                                                                                               aTGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTGCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGa                    >  2:251698/1‑148 (MQ=255)
                                                                                                                                 gatACTCGCGGGGGTCGTGCTACCACTGGTAAACATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                  <  1:934387/146‑1 (MQ=255)
                                                                                                                                 gCTACTCTCGGTGGTCGCCGTACCATTAATAAAAACATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                  >  2:1360233/1‑148 (MQ=255)
                                                                                                                                 gCTACTCACGGTGGTAGTGATAAAACTGGTAAAAATGTACATGTTGGGGCTGGCGCAGTATTAGCAGGTGTGATTGATGCCCCTAGTGCTTCACCGGTTATAATAGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                  <  2:1056264/148‑1 (MQ=255)
                                                                                                                                  cTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAgg                 >  1:1149075/1‑148 (MQ=255)
                                                                                                                                  cTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTACTCGGTGCCAATGAAGTTATTTTAGAAgg                 >  2:373020/1‑148 (MQ=255)
                                                                                                                                       ctcGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTAc            >  1:1376223/1‑148 (MQ=255)
                                                                                                                                            tggtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtg       >  1:616188/1‑147 (MQ=255)
                                                                                                                                              gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg     <  1:1028025/148‑1 (MQ=255)
                                                                                                                                              gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg     <  2:1164182/148‑1 (MQ=255)
                                                                                                                                              gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg     <  1:1238844/148‑1 (MQ=255)
                                                                                                                                              gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg     <  2:179423/148‑1 (MQ=255)
                                                                                                                                              gacGTGCTACAACTGGTAAAAATGAACAGGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGttag     <  2:370597/146‑3 (MQ=255)
                                                                                                                                                 gTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATTTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtcgccca  >  2:961242/1‑142 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAA  >  USA300TCH1516_ALE/1434917‑1435209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑