Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,435,0690AC22.4% 215.7 / 12.0 108T142T (ACA→ACC) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (32/51);  new base C (23/1);  total (56/52)
Fisher's exact test for biased strand distribution p-value = 2.38e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

CGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCT  >  USA300TCH1516_ALE/1434923‑1435216
                                                                                                                                                  |                                                                                                                                                   
cGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGGTACaa                                                                                                                                                    >  2:1162886/1‑148 (MQ=255)
cGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACaa                                                                                                                                                    >  1:268185/1‑148 (MQ=255)
  aaTTGAACGAGGTGCCTTTATTAGAGAACAAGACATTATCGAAGATGGTTCTGTGGTTATGATGGGCGCAAAGATGAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACt                                                                                                                                                  <  2:250464/148‑1 (MQ=255)
      gAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTa                                                                                                                                              <  1:589220/148‑1 (MQ=255)
         ccAGGAGCGTTTATTAGAGAAAAAGACATTATTGAAGATGGTGCTGTCGTTATTATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTaaaa                                                                                                                                           <  2:480357/148‑1 (MQ=255)
             gTGCGTTTATTAGAGAACCAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGt                                                                                                                                       <  1:1071505/148‑1 (MQ=255)
              tGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTa                                                                                                                                      <  1:582662/148‑1 (MQ=255)
                cGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACa                                                                                                                                    <  1:1142718/148‑1 (MQ=255)
                 gTTTATTAGATAACAAGCCATTATTGAAGATGGTGCTGGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACAt                                                                                                                                   <  1:1196064/148‑1 (MQ=255)
                      ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCACTAGAAAAAAAGAACACGTAgg                                                                                                                              >  2:646413/1‑148 (MQ=255)
                      ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGCTATAACTAGAAAAAAGATACATgcaca                                                                                                                              >  2:646412/1‑144 (MQ=255)
                       taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTCGTCGTGCTACAACTGGTAAAAATGTACATGTAggg                                                                                                                             >  1:781808/1‑148 (MQ=255)
                        agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                            >  1:1216444/1‑148 (MQ=255)
                        agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTATCATTAGAAAAAAAGTACATGTAgggg                                                                                                                            >  2:694331/1‑148 (MQ=255)
                         gagaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAAGGTACATGTAGGGGc                                                                                                                           >  1:1235550/1‑148 (MQ=255)
                          agaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCt                                                                                                                          >  1:791070/1‑148 (MQ=255)
                            aaCGAGCCATTATTTAAGATGGTGCTTGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                        <  2:803735/148‑1 (MQ=255)
                               aaGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACAACTAAAAAAAAAATACATGAAGGGGCTGgcgc                                                                                                                     >  2:1149/1‑148 (MQ=255)
                                 gcaatttttGAAGATGGTGGTGTCGTTAGGATGGGCGCAACAATTAATATTGGGGCAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                   <  1:37915/145‑1 (MQ=255)
                                 gCCATTATTGAAGATGGTGCTGTCGTTATGAGGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                   <  1:582414/148‑1 (MQ=255)
                                 gCCATTAGTGAATAAGGTGGTGTCGTTATGAAGGGCGCAACAATTAATATTGGCACAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                   <  2:135826/148‑1 (MQ=255)
                                   caattatGAAGATGGGGCTATCGGTATGATGGGCGTAACAATTAATGTTGGCGCAGTCGTTGTCGAAGGTACAATGATGGATATGAATGCTACTCGCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                 <  2:707239/142‑1 (MQ=255)
                                   cATAATAGAAGATGGTGCTGTCGTAATGCAGGGCGCAACAATGGATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGACTGCTTCTGTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                 <  2:1285435/148‑1 (MQ=255)
                                    attattGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGTAGTACCGATGAAAGGTAACGTGCATTACACGAAGGCTACTCTCGTTGATCGTGCTACGACTGATAGAAATGTACATGTGGGGGCTGGCGCAGTAt                                                                                                                >  1:1392300/1‑148 (MQ=255)
                                       attTAAGATGGTTCTAGCGGTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                             <  2:1184297/148‑1 (MQ=255)
                                       attGAAGATGGGGCATTCGTTATGATGGGTGCAACAATTAATGTTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                             <  2:1338680/148‑1 (MQ=255)
                                        ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTCGGGGCTGGCGCAGTATTAGc                                                                                                            >  1:179423/1‑148 (MQ=255)
                                        ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                            >  1:806476/1‑148 (MQ=255)
                                        ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                            >  1:1164182/1‑148 (MQ=255)
                                         tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                           >  1:420433/1‑148 (MQ=255)
                                         tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                           >  1:1329508/1‑148 (MQ=255)
                                          gAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTGAAAAAAATGTACATGTAGGGGCTGGCACAGTATTAGCAg                                                                                                          >  2:169231/1‑148 (MQ=255)
                                           aaGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAGTAAAAAAAAAATACATGTAGGGGCTGGCGAAGTAAGAGCacg                                                                                                         >  2:1227963/1‑146 (MQ=255)
                                            aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACACGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                        >  1:661539/1‑148 (MQ=255)
                                            aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                        >  2:655407/1‑148 (MQ=255)
                                            aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAggg                                                                                                        >  2:894240/1‑147 (MQ=255)
                                               tGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGtgtg                                                                                                     <  1:1174347/148‑1 (MQ=255)
                                                 gtccTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATTAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCATTATTAGCAGGTGTGAt                                                                                                   <  1:695697/145‑1 (MQ=255)
                                                 gTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGAt                                                                                                   <  1:89040/148‑1 (MQ=255)
                                                   gCTGTCGTTATGATGGGCGCAACAATTAATATGGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGGATTAGCAGGTGTGAGTg                                                                                                 >  1:146675/1‑148 (MQ=255)
                                                        cGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAccc                                                                                            >  1:600991/1‑148 (MQ=255)
                                                         gTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGTTATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                           <  2:755301/148‑1 (MQ=255)
                                                         gTTATGATGGGCGCAACAATTAACATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                           <  1:55719/148‑1 (MQ=255)
                                                         gTTATAAGGGGCGTAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                           <  2:840938/148‑1 (MQ=255)
                                                                    cgcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAAAACATGTAGGGGCCGGCGCAGTACTAGCAAGATTGATTGAACCCCCTACTGcagca                                                                                >  2:1087074/1‑144 (MQ=255)
                                                                     gaaacaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                               <  1:591901/146‑1 (MQ=255)
                                                                     acaacaaATAAAACTAGCGCAGTCGTTAGCAAAGAAACAACGATTAATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                               <  1:375753/147‑1 (MQ=255)
                                                                        acaaTTAATATTGGCGCAGTCGTTAGCGGAGGTATAATGATTGATATAAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCgg                                                                            <  1:533733/148‑1 (MQ=255)
                                                                         caaTTAATATTGGTGCGGTGGTTGGCGAAGGTACAAGGATTGATATGAATGCTGCCCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGTTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGt                                                                           <  1:46820/148‑1 (MQ=255)
                                                                          caTTAATATGTGCCAAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTCGCAGGCGCGATTGCACCCCCTAGTGCTTCACCGGtt                                                                          <  2:1398803/147‑1 (MQ=255)
                                                                          aaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCTGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                          <  1:1331992/148‑1 (MQ=255)
                                                                          aaTTAATATGGGCGCAGTCGTTGGCGAGGGTGCAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                          <  2:220957/148‑1 (MQ=255)
                                                                               atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                                     <  1:156017/148‑1 (MQ=255)
                                                                               atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCCTATCACTAATAAAAATATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                                     >  2:418411/1‑148 (MQ=255)
                                                                                   tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTACCATTAAAAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAg                                                                 >  2:1245472/1‑148 (MQ=255)
                                                                                   tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCACCTAGTGCTTCACCGGTAATAAGCGAg                                                                 >  2:1069349/1‑148 (MQ=255)
                                                                                     gcgcAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGAATTATCAGGTGGGATTGAAGCCCCTAGTGCTTCAACGGTTATAAACGAGga                                                               >  2:211889/1‑148 (MQ=255)
                                                                                         aGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTCTAATCGAGgatgat                                                           >  1:113084/1‑148 (MQ=255)
                                                                                         aGTCGTTGGCGAAGGTACAATGATTGAAATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGgatgat                                                           <  1:979172/148‑1 (MQ=255)
                                                                                            cGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGAGGATGTa                                                        >  1:766570/1‑148 (MQ=255)
                                                                                               tGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAGCGAGGATGATGTATTa                                                     <  2:1005977/148‑1 (MQ=255)
                                                                                                 gCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAAGAGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTaaa                                                   >  2:254310/1‑147 (MQ=255)
                                                                                                  cGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCGGTATTAGCAGGTGTGATTGAACCCCCTAGAGCTGCACCGGTTATAAACGAGGATGATGTATTAATc                                                  >  2:187177/1‑148 (MQ=255)
                                                                                                   gAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGTTGTGATTGAACCCCCTAGTGCTTCCCCGGTTATACTCGAGGATGATGTATTAATCg                                                 >  2:1012947/1‑148 (MQ=255)
                                                                                                   gAAGGTACAATGATTGATATGAATGCTACTCTCAGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTAATCg                                                 >  1:703971/1‑148 (MQ=255)
                                                                                                    aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCgg                                                >  1:1236822/1‑148 (MQ=255)
                                                                                                    aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGAAGTATTAATCgg                                                >  1:593190/1‑148 (MQ=255)
                                                                                                     aGGTAAAATGATTGAGAGGAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGt                                               <  2:456353/148‑1 (MQ=255)
                                                                                                        tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTGAAAAAAAAATACATATAGGGGCTGGAGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCTGTTATAATAGAGAACGATGGATTAATCgggtca                                            >  2:1111803/1‑144 (MQ=255)
                                                                                                        tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAAAACTAGGGGCACGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATCTATGAATCCGTGCa                                            >  2:1146062/1‑148 (MQ=255)
                                                                                                         aaaatgaatgATATAAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCaa                                           <  2:1039641/146‑1 (MQ=255)
                                                                                                          cAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGTACATGTAGGGGCGGGCGCAGTATTAGCAGGTGTGATTGAACCCGCTAGTGCTAAAGCGATAATAATCGAGGATGATGTATTAATCGGTGCaac                                          >  2:1053515/1‑147 (MQ=255)
                                                                                                              gattaacATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                                      <  1:599358/148‑1 (MQ=255)
                                                                                                              gaatgaaATAAATGCAACACGCGGTGGTCGGGCTACAACTGGTAAAAATGTACATGAAGGGGCTCGCTCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                                      <  2:670693/145‑1 (MQ=255)
                                                                                                                ttgatATGAATGCTCCTCTCGGTGGTCGTGCTACAACTCGTAAAAATGTACATGTAGAGACTGTCGCATTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGt                                    <  1:1386660/148‑1 (MQ=255)
                                                                                                                     atGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                               <  1:1149/148‑1 (MQ=255)
                                                                                                                     atGAAGGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                               <  1:981270/148‑1 (MQ=255)
                                                                                                                      tGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAtttt                              >  1:1413360/1‑148 (MQ=255)
                                                                                                                       gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCACTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCGAATGCAGTTATTTTa                             >  2:937542/1‑148 (MQ=255)
                                                                                                                       gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGAAAGTGAGATTGAACCCCCTATTGCTTCTCCGGTTATAATCGAGGGTGATGTCTTATTAGGTGCAAATGGAGTTAGTTTa                             >  2:937691/1‑148 (MQ=255)
                                                                                                                        aaTGTTACTCTCGGTGGTCGTGCTACAACTTGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGGACCGCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTGtttggg                            <  2:870104/148‑4 (MQ=255)
                                                                                                                         aTGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTGCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGa                           >  2:251698/1‑148 (MQ=255)
                                                                                                                           gatACTCGCGGGGGTCGTGCTACCACTGGTAAACATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                         <  1:934387/146‑1 (MQ=255)
                                                                                                                           gCTACTCTCGGTGGTCGCCGTACCATTAATAAAAACATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                         >  2:1360233/1‑148 (MQ=255)
                                                                                                                            cTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAgg                        >  1:1149075/1‑148 (MQ=255)
                                                                                                                            cTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTACTCGGTGCCAATGAAGTTATTTTAGAAgg                        >  2:373020/1‑148 (MQ=255)
                                                                                                                                 ctcGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTAc                   >  1:1376223/1‑148 (MQ=255)
                                                                                                                                        gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCTCCTAGTGCTTCACCGGTTATAATAGAGGATGATGTATTAATCTGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg            <  2:682586/148‑1 (MQ=255)
                                                                                                                                        gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg            <  1:1238844/148‑1 (MQ=255)
                                                                                                                                        gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg            <  2:1164182/148‑1 (MQ=255)
                                                                                                                                        gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg            <  1:1028025/148‑1 (MQ=255)
                                                                                                                                        gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg            <  2:179423/148‑1 (MQ=255)
                                                                                                                                        gacGTGCTACAACTGGTAAAAATGAACAGGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGttag            <  2:370597/146‑3 (MQ=255)
                                                                                                                                           gTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATTTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtcgccca         >  2:961242/1‑142 (MQ=255)
                                                                                                                                                aCAACTGGTAAAAATGTACCTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg    <  1:151284/148‑1 (MQ=255)
                                                                                                                                                aCAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg    <  2:1236822/148‑1 (MQ=255)
                                                                                                                                                aCAACGGGTAAACATGTTCATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg    <  2:593190/148‑1 (MQ=255)
                                                                                                                                                  aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt  >  1:1090948/1‑148 (MQ=255)
                                                                                                                                                  aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt  >  1:257157/1‑148 (MQ=255)
                                                                                                                                                  aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt  >  2:38092/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCT  >  USA300TCH1516_ALE/1434923‑1435216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑