Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,435,0750TA22.5% 255.7 / 17.5 111G144G (GGT→GGA) ‡dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (35/51);  new base A (25/0);  total (60/51)
Fisher's exact test for biased strand distribution p-value = 1.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 111 total aligned reads displayed.

GAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATT  >  USA300TCH1516_ALE/1434929‑1435219
                                                                                                                                                  |                                                                                                                                                
gAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTa                                                                                                                                                 <  1:589220/148‑1 (MQ=255)
   ccAGGAGCGTTTATTAGAGAAAAAGACATTATTGAAGATGGTGCTGTCGTTATTATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTaaaa                                                                                                                                              <  2:480357/148‑1 (MQ=255)
       gTGCGTTTATTAGAGAACCAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGt                                                                                                                                          <  1:1071505/148‑1 (MQ=255)
        tGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTa                                                                                                                                         <  1:582662/148‑1 (MQ=255)
          cGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACa                                                                                                                                       <  1:1142718/148‑1 (MQ=255)
                ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCACTAGAAAAAAAGAACACGTAgg                                                                                                                                 >  2:646413/1‑148 (MQ=255)
                ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGCTATAACTAGAAAAAAGATACATgcaca                                                                                                                                 >  2:646412/1‑144 (MQ=255)
                 taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTCGTCGTGCTACAACTGGTAAAAATGTACATGTAggg                                                                                                                                >  1:781808/1‑148 (MQ=255)
                 taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTGCAACTGGTAAAAATGTACATGTAggg                                                                                                                                >  1:1335402/1‑148 (MQ=255)
                  agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                               >  1:1216444/1‑148 (MQ=255)
                  agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTATCATTAGAAAAAAAGTACATGTAgggg                                                                                                                               >  2:694331/1‑148 (MQ=255)
                  agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTCCAATTAAAAAAAAAATACATTTAgggg                                                                                                                               >  2:1200304/1‑148 (MQ=255)
                   gagaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAAGGTACATGTAGGGGc                                                                                                                              >  1:1235550/1‑148 (MQ=255)
                    agaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCt                                                                                                                             >  1:791070/1‑148 (MQ=255)
                      aaaaaGCCATTATTGAAGATGGTGCTGTCGTTTTGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACACTGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                           <  2:786073/145‑1 (MQ=255)
                      aaCGAGCCATTATTTAAGATGGTGCTTGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                           <  2:803735/148‑1 (MQ=255)
                         aaGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACAACTAAAAAAAAAATACATGAAGGGGCTGgcgc                                                                                                                        >  2:1149/1‑148 (MQ=255)
                           gcaatttttGAAGATGGTGGTGTCGTTAGGATGGGCGCAACAATTAATATTGGGGCAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                      <  1:37915/145‑1 (MQ=255)
                           gCCATTATTGAAGATGGTGCTGTCGTTATGAGGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                      <  1:582414/148‑1 (MQ=255)
                           gCCATTAGTGAATAAGGTGGTGTCGTTATGAAGGGCGCAACAATTAATATTGGCACAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                      <  2:135826/148‑1 (MQ=255)
                             cATAATAGAAGATGGTGCTGTCGTAATGCAGGGCGCAACAATGGATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGACTGCTTCTGTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                    <  2:1285435/148‑1 (MQ=255)
                                 attTAAGATGGTTCTAGCGGTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                                <  2:1184297/148‑1 (MQ=255)
                                 attGAAGATGGGGCATTCGTTATGATGGGTGCAACAATTAATGTTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                                <  2:1338680/148‑1 (MQ=255)
                                  ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTCGGGGCTGGCGCAGTATTAGc                                                                                                               >  1:179423/1‑148 (MQ=255)
                                  ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                               >  1:1164182/1‑148 (MQ=255)
                                   tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                              >  1:1167532/1‑148 (MQ=255)
                                   tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                              >  1:1329508/1‑148 (MQ=255)
                                   tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACATATAGGGGCTGGCGCAGTATTAGcg                                                                                                              >  2:25179/1‑147 (MQ=255)
                                    gAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTGAAAAAAATGTACATGTAGGGGCTGGCACAGTATTAGCAg                                                                                                             >  2:169231/1‑148 (MQ=255)
                                     aaGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAGTAAAAAAAAAATACATGTAGGGGCTGGCGAAGTAAGAGCacg                                                                                                            >  2:1227963/1‑146 (MQ=255)
                                      aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACACGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                           >  1:661539/1‑148 (MQ=255)
                                      aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                           >  2:655407/1‑148 (MQ=255)
                                      aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAggg                                                                                                           >  2:894240/1‑147 (MQ=255)
                                      aGATGGGGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATAATTGATATGAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                           <  2:1412530/148‑1 (MQ=255)
                                         tGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGtgtg                                                                                                        <  1:1174347/148‑1 (MQ=255)
                                           gtccTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATTAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCATTATTAGCAGGTGTGAt                                                                                                      <  1:695697/145‑1 (MQ=255)
                                           gTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGAt                                                                                                      <  1:89040/148‑1 (MQ=255)
                                             gCTGTCGTTATGATGGGCGCAACAATTAATATGGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGGATTAGCAGGTGTGAGTg                                                                                                    >  1:146675/1‑148 (MQ=255)
                                                gTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAc                                                                                                 >  1:549060/1‑148 (MQ=255)
                                                gTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGAGGCTGGCGCAGTATTAGCAGGTGTGATTGAAc                                                                                                 >  1:243900/1‑148 (MQ=255)
                                                  cGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAccc                                                                                               >  1:600991/1‑148 (MQ=255)
                                                   gTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGTTATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                              <  2:755301/148‑1 (MQ=255)
                                                   gTTATGATGGGCGCAACAATTAACATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                              <  1:55719/148‑1 (MQ=255)
                                                   gTTATAAGGGGCGTAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                              <  2:840938/148‑1 (MQ=255)
                                                        gatgGGCGCAACAATTAATATTGGCGCAGTCGTTGGAGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGt                                                                                         >  1:894965/1‑148 (MQ=255)
                                                              cgcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAAAACATGTAGGGGCCGGCGCAGTACTAGCAAGATTGATTGAACCCCCTACTGcagca                                                                                   >  2:1087074/1‑144 (MQ=255)
                                                               gaaacaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                                  <  1:591901/146‑1 (MQ=255)
                                                               acaacaaATAAAACTAGCGCAGTCGTTAGCAAAGAAACAACGATTAATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                                  <  1:375753/147‑1 (MQ=255)
                                                                  acaaTTAATATTGGCGCAGTCGTTAGCGGAGGTATAATGATTGATATAAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCgg                                                                               <  1:533733/148‑1 (MQ=255)
                                                                   caaTTAATATTGGTGCGGTGGTTGGCGAAGGTACAAGGATTGATATGAATGCTGCCCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGTTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGt                                                                              <  1:46820/148‑1 (MQ=255)
                                                                    caTTAATATGTGCCAAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTCGCAGGCGCGATTGCACCCCCTAGTGCTTCACCGGtt                                                                             <  2:1398803/147‑1 (MQ=255)
                                                                    aaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCTGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                             <  1:1331992/148‑1 (MQ=255)
                                                                    aaTTAATATGGGCGCAGTCGTTGGCGAGGGTGCAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                             <  2:220957/148‑1 (MQ=255)
                                                                         atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                                        <  1:156017/148‑1 (MQ=255)
                                                                         atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCCTATCACTAATAAAAATATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                                        >  2:418411/1‑148 (MQ=255)
                                                                             tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTACCATTAAAAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAg                                                                    >  2:1245472/1‑148 (MQ=255)
                                                                             tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCACCTAGTGCTTCACCGGTAATAAGCGAg                                                                    >  2:1069349/1‑148 (MQ=255)
                                                                               gcgcAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGAATTATCAGGTGGGATTGAAGCCCCTAGTGCTTCAACGGTTATAAACGAGga                                                                  >  2:211889/1‑148 (MQ=255)
                                                                                   aGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTCTAATCGAGgatgat                                                              >  1:113084/1‑148 (MQ=255)
                                                                                   aGTCGTTGGCGAAGGTACAATGATTGAAATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGgatgat                                                              <  1:979172/148‑1 (MQ=255)
                                                                                      cGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGAGGATGTa                                                           >  1:766570/1‑148 (MQ=255)
                                                                                         tGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAGCGAGGATGATGTATTa                                                        <  2:1005977/148‑1 (MQ=255)
                                                                                           gCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAAGAGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTaaa                                                      >  2:254310/1‑147 (MQ=255)
                                                                                             gAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGTTGTGATTGAACCCCCTAGTGCTTCCCCGGTTATACTCGAGGATGATGTATTAATCg                                                    >  2:1012947/1‑148 (MQ=255)
                                                                                             gAAGGTACAATGATTGATATGAATGCTACTCTCAGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTAATCg                                                    >  1:703971/1‑148 (MQ=255)
                                                                                              aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCgg                                                   >  1:1236822/1‑148 (MQ=255)
                                                                                              aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGAAGTATTAATCgg                                                   >  1:593190/1‑148 (MQ=255)
                                                                                                  tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTGAAAAAAAAATACATATAGGGGCTGGAGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCTGTTATAATAGAGAACGATGGATTAATCgggtca                                               >  2:1111803/1‑144 (MQ=255)
                                                                                                  tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAAAACTAGGGGCACGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATCTATGAATCCGTGCa                                               >  2:1146062/1‑148 (MQ=255)
                                                                                                   aaaatgaatgATATAAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCaa                                              <  2:1039641/146‑1 (MQ=255)
                                                                                                    cAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGTACATGTAGGGGCGGGCGCAGTATTAGCAGGTGTGATTGAACCCGCTAGTGCTAAAGCGATAATAATCGAGGATGATGTATTAATCGGTGCaac                                             >  2:1053515/1‑147 (MQ=255)
                                                                                                        gaatgaaATAAATGCAACACGCGGTGGTCGGGCTACAACTGGTAAAAATGTACATGAAGGGGCTCGCTCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                                         <  2:670693/145‑1 (MQ=255)
                                                                                                          ttgatATGAATGCTCCTCTCGGTGGTCGTGCTACAACTCGTAAAAATGTACATGTAGAGACTGTCGCATTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGt                                       <  1:1386660/148‑1 (MQ=255)
                                                                                                               atGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                                  <  1:1149/148‑1 (MQ=255)
                                                                                                               atGAAGGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                                  <  1:981270/148‑1 (MQ=255)
                                                                                                                tGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAtttt                                 >  1:1413360/1‑148 (MQ=255)
                                                                                                                 gAATGCTACTCTCGGTGGTCGTCGTATCATTGAAAAAAAAGTACATGTAGGGACTGGCGCAGCATTCGAAGGTGTGCTTGAACCCCCTAATGGTTGCACGGTTGTAATGGAGGATGATGTATTAATCGTTGCAAATGAAGATATTTTa                                >  2:616418/1‑148 (MQ=255)
                                                                                                                 gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCACTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCGAATGCAGTTATTTTa                                >  2:937542/1‑148 (MQ=255)
                                                                                                                 gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGAAAGTGAGATTGAACCCCCTATTGCTTCTCCGGTTATAATCGAGGGTGATGTCTTATTAGGTGCAAATGGAGTTAGTTTa                                >  2:937691/1‑148 (MQ=255)
                                                                                                                  aaTGTTACTCTCGGTGGTCGTGCTACAACTTGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGGACCGCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTGtttggg                               <  2:870104/148‑4 (MQ=255)
                                                                                                                   aTGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTGCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGa                              >  2:251698/1‑148 (MQ=255)
                                                                                                                     gatACTCGCGGGGGTCGTGCTACCACTGGTAAACATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                            <  1:934387/146‑1 (MQ=255)
                                                                                                                     gCTACTCTCGGTGGTCGCCGTACCATTAATAAAAACATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                            >  2:1360233/1‑148 (MQ=255)
                                                                                                                      cTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAgg                           >  1:1149075/1‑148 (MQ=255)
                                                                                                                      cTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTACTCGGTGCCAATGAAGTTATTTTAGAAgg                           >  2:373020/1‑148 (MQ=255)
                                                                                                                           ctcGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTAc                      >  1:1376223/1‑148 (MQ=255)
                                                                                                                                tggtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtg                 >  1:616188/1‑147 (MQ=255)
                                                                                                                                  gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCTCCTAGTGCTTCACCGGTTATAATAGAGGATGATGTATTAATCTGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg               <  2:682586/148‑1 (MQ=255)
                                                                                                                                  gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg               <  2:179423/148‑1 (MQ=255)
                                                                                                                                  gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg               <  1:1238844/148‑1 (MQ=255)
                                                                                                                                  gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg               <  1:1028025/148‑1 (MQ=255)
                                                                                                                                  gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg               <  2:1164182/148‑1 (MQ=255)
                                                                                                                                  gacGTGCTACAACTGGTAAAAATGAACAGGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGttag               <  2:370597/146‑3 (MQ=255)
                                                                                                                                     gTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATTTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtcgccca            >  2:961242/1‑142 (MQ=255)
                                                                                                                                          aCAACTGGTAAAAATGTACCTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg       <  1:151284/148‑1 (MQ=255)
                                                                                                                                          aCAACGGGTAAACATGTTCATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg       <  2:593190/148‑1 (MQ=255)
                                                                                                                                            aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt     >  1:1090948/1‑148 (MQ=255)
                                                                                                                                            aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt     >  1:257157/1‑148 (MQ=255)
                                                                                                                                            aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt     >  2:38092/1‑148 (MQ=255)
                                                                                                                                               tGGTAAAAATGTACATGTAGGGGCTGGCGAAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATAGATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTCtt  >  1:728382/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATT  >  USA300TCH1516_ALE/1434929‑1435219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑