Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,435,0810TA22.4% 236.2 / 21.3 110N146K (AAT→AAA) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (33/50);  new base A (24/0);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 2.75e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 110 total aligned reads displayed.

GTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGTTGC  >  USA300TCH1516_ALE/1434936‑1435224
                                                                                                                                                 |                                                                                                                                               
gTGCGTTTATTAGAGAACCAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGt                                                                                                                                               <  1:1071505/148‑1 (MQ=255)
 tGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTa                                                                                                                                              <  1:582662/148‑1 (MQ=255)
   cGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACa                                                                                                                                            <  1:1142718/148‑1 (MQ=255)
         ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCACTAGAAAAAAAGAACACGTAgg                                                                                                                                      >  2:646413/1‑148 (MQ=255)
         ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGCTATAACTAGAAAAAAGATACATgcaca                                                                                                                                      >  2:646412/1‑144 (MQ=255)
          taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTCGTCGTGCTACAACTGGTAAAAATGTACATGTAggg                                                                                                                                     >  1:781808/1‑148 (MQ=255)
          taGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTGCAACTGGTAAAAATGTACATGTAggg                                                                                                                                     >  1:1335402/1‑148 (MQ=255)
           agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                                    >  1:1216444/1‑148 (MQ=255)
           agagAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTCCAATTAAAAAAAAAATACATTTAgggg                                                                                                                                    >  2:1200304/1‑148 (MQ=255)
            gagaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAAGGTACATGTAGGGGc                                                                                                                                   >  1:1235550/1‑148 (MQ=255)
             agaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCt                                                                                                                                  >  1:791070/1‑148 (MQ=255)
               aaaaaGCCATTATTGAAGATGGTGCTGTCGTTTTGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACACTGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                                <  2:786073/145‑1 (MQ=255)
               aaCGAGCCATTATTTAAGATGGTGCTTGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTgg                                                                                                                                <  2:803735/148‑1 (MQ=255)
                    gcaatttttGAAGATGGTGGTGTCGTTAGGATGGGCGCAACAATTAATATTGGGGCAGTCGTTGGCGAAGGTAAAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                           <  1:37915/145‑1 (MQ=255)
                    gCCATTATTGAAGATGGTGCTGTCGTTATGAGGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                           <  1:582414/148‑1 (MQ=255)
                    gCCATTAGTGAATAAGGTGGTGTCGTTATGAAGGGCGCAACAATTAATATTGGCACAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAg                                                                                                                           <  2:135826/148‑1 (MQ=255)
                      caattatGAAGATGGGGCTATCGGTATGATGGGCGTAACAATTAATGTTGGCGCAGTCGTTGTCGAAGGTACAATGATGGATATGAATGCTACTCGCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                         <  2:707239/142‑1 (MQ=255)
                      cATAATAGAAGATGGTGCTGTCGTAATGCAGGGCGCAACAATGGATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGACTGCTTCTGTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                         <  2:1285435/148‑1 (MQ=255)
                       attattGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGTAGTACCGATGAAAGGTAACGTGCATTACACGAAGGCTACTCTCGTTGATCGTGCTACGACTGATAGAAATGTACATGTGGGGGCTGGCGCAGTAt                                                                                                                        >  1:1392300/1‑148 (MQ=255)
                          attTAAGATGGTTCTAGCGGTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGGGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                                     <  2:1184297/148‑1 (MQ=255)
                          attGAAGATGGGGCATTCGTTATGATGGGTGCAACAATTAATGTTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAg                                                                                                                     <  2:1338680/148‑1 (MQ=255)
                           ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTCGGGGCTGGCGCAGTATTAGc                                                                                                                    >  1:179423/1‑148 (MQ=255)
                           ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                                    >  1:806476/1‑148 (MQ=255)
                           ttGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGc                                                                                                                    >  1:1164182/1‑148 (MQ=255)
                            tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                                   >  1:1167532/1‑148 (MQ=255)
                            tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                                   >  1:1329508/1‑148 (MQ=255)
                            tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa                                                                                                                   >  1:420433/1‑148 (MQ=255)
                            tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACATATAGGGGCTGGCGCAGTATTAGcg                                                                                                                   >  2:25179/1‑147 (MQ=255)
                             gAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTGAAAAAAATGTACATGTAGGGGCTGGCACAGTATTAGCAg                                                                                                                  >  2:169231/1‑148 (MQ=255)
                              aaGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAGTAAAAAAAAAATACATGTAGGGGCTGGCGAAGTAAGAGCacg                                                                                                                 >  2:1227963/1‑146 (MQ=255)
                               aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                                >  2:655407/1‑148 (MQ=255)
                               aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAggg                                                                                                                >  2:894240/1‑147 (MQ=255)
                               aGATGGGGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATAATTGATATGAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                                <  2:1412530/148‑1 (MQ=255)
                                  tGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGtgtg                                                                                                             <  1:1174347/148‑1 (MQ=255)
                                    gtccTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATTAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCATTATTAGCAGGTGTGAt                                                                                                           <  1:695697/145‑1 (MQ=255)
                                    gTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGAt                                                                                                           <  1:89040/148‑1 (MQ=255)
                                      gCTGTCGTTATGATGGGCGCAACAATTAATATGGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGGATTAGCAGGTGTGAGTg                                                                                                         >  1:146675/1‑148 (MQ=255)
                                         gTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAc                                                                                                      >  1:549060/1‑148 (MQ=255)
                                         gTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGAGGCTGGCGCAGTATTAGCAGGTGTGATTGAAc                                                                                                      >  1:243900/1‑148 (MQ=255)
                                           cGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAccc                                                                                                    >  1:600991/1‑148 (MQ=255)
                                            gTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGTTATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                                   <  2:755301/148‑1 (MQ=255)
                                            gTTATGATGGGCGCAACAATTAACATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                                   <  1:55719/148‑1 (MQ=255)
                                            gTTATAAGGGGCGTAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAcccc                                                                                                   <  2:840938/148‑1 (MQ=255)
                                                 gatgGGCGCAACAATTAATATTGGCGCAGTCGTTGGAGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGt                                                                                              >  1:894965/1‑148 (MQ=255)
                                                       cgcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAAAACATGTAGGGGCCGGCGCAGTACTAGCAAGATTGATTGAACCCCCTACTGcagca                                                                                        >  2:1087074/1‑144 (MQ=255)
                                                        gaaacaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                                       <  1:591901/146‑1 (MQ=255)
                                                        acaacaaATAAAACTAGCGCAGTCGTTAGCAAAGAAACAACGATTAATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCAc                                                                                       <  1:375753/147‑1 (MQ=255)
                                                           acaaTTAATATTGGCGCAGTCGTTAGCGGAGGTATAATGATTGATATAAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCgg                                                                                    <  1:533733/148‑1 (MQ=255)
                                                             aaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCTGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                                  <  1:1331992/148‑1 (MQ=255)
                                                             aaTTAATATGGGCGCAGTCGTTGGCGAGGGTGCAATGATTGAGATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGtt                                                                                  <  2:220957/148‑1 (MQ=255)
                                                                  atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                                             <  1:156017/148‑1 (MQ=255)
                                                                  atatTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCCTATCACTAATAAAAATATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAt                                                                             >  2:418411/1‑148 (MQ=255)
                                                                      tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTCGTACCATTAAAAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAg                                                                         >  2:1245472/1‑148 (MQ=255)
                                                                      tGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCACCTAGTGCTTCACCGGTAATAAGCGAg                                                                         >  2:1069349/1‑148 (MQ=255)
                                                                        gcgcAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGAATTATCAGGTGGGATTGAAGCCCCTAGTGCTTCAACGGTTATAAACGAGga                                                                       >  2:211889/1‑148 (MQ=255)
                                                                            aGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTCTAATCGAGgatgat                                                                   >  1:113084/1‑148 (MQ=255)
                                                                               cGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGAGGATGTa                                                                >  1:766570/1‑148 (MQ=255)
                                                                                  tGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAGCGAGGATGATGTATTa                                                             <  2:1005977/148‑1 (MQ=255)
                                                                                    gCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAAGAGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTaaa                                                           >  2:254310/1‑147 (MQ=255)
                                                                                     cGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCGGTATTAGCAGGTGTGATTGAACCCCCTAGAGCTGCACCGGTTATAAACGAGGATGATGTATTAATc                                                          >  2:187177/1‑148 (MQ=255)
                                                                                      gAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGTTGTGATTGAACCCCCTAGTGCTTCCCCGGTTATACTCGAGGATGATGTATTAATCg                                                         >  2:1012947/1‑148 (MQ=255)
                                                                                      gAAGGTACAATGATTGATATGAATGCTACTCTCAGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGGTGATGTATTAATCg                                                         >  1:703971/1‑148 (MQ=255)
                                                                                       aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCgg                                                        >  1:1236822/1‑148 (MQ=255)
                                                                                       aaGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGAAGTATTAATCgg                                                        >  1:593190/1‑148 (MQ=255)
                                                                                        aGGTAAAATGATTGAGAGGAATGCGACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGt                                                       <  2:456353/148‑1 (MQ=255)
                                                                                           tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTGAAAAAAAAATACATATAGGGGCTGGAGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCTGTTATAATAGAGAACGATGGATTAATCgggtca                                                    >  2:1111803/1‑144 (MQ=255)
                                                                                           tACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAAAACTAGGGGCACGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATCTATGAATCCGTGCa                                                    >  2:1146062/1‑148 (MQ=255)
                                                                                            aaaatgaatgATATAAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCaa                                                   <  2:1039641/146‑1 (MQ=255)
                                                                                             cAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGTACATGTAGGGGCGGGCGCAGTATTAGCAGGTGTGATTGAACCCGCTAGTGCTAAAGCGATAATAATCGAGGATGATGTATTAATCGGTGCaac                                                  >  2:1053515/1‑147 (MQ=255)
                                                                                                 gattaacATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                                              <  1:599358/148‑1 (MQ=255)
                                                                                                 gaatgaaATAAATGCAACACGCGGTGGTCGGGCTACAACTGGTAAAAATGTACATGAAGGGGCTCGCTCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCa                                              <  2:670693/145‑1 (MQ=255)
                                                                                                   ttgatATGAATGCTCCTCTCGGTGGTCGTGCTACAACTCGTAAAAATGTACATGTAGAGACTGTCGCATTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGt                                            <  1:1386660/148‑1 (MQ=255)
                                                                                                        atGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                                       <  1:1149/148‑1 (MQ=255)
                                                                                                        atGAAGGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAttt                                       <  1:981270/148‑1 (MQ=255)
                                                                                                          gAATGCTACTCTCGGTGGTCGTCGTATCATTGAAAAAAAAGTACATGTAGGGACTGGCGCAGCATTCGAAGGTGTGCTTGAACCCCCTAATGGTTGCACGGTTGTAATGGAGGATGATGTATTAATCGTTGCAAATGAAGATATTTTa                                     >  2:616418/1‑148 (MQ=255)
                                                                                                          gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCACTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCGAATGCAGTTATTTTa                                     >  2:937542/1‑148 (MQ=255)
                                                                                                          gAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGAAAGTGAGATTGAACCCCCTATTGCTTCTCCGGTTATAATCGAGGGTGATGTCTTATTAGGTGCAAATGGAGTTAGTTTa                                     >  2:937691/1‑148 (MQ=255)
                                                                                                           aaTGTTACTCTCGGTGGTCGTGCTACAACTTGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGGACCGCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTGtttggg                                    <  2:870104/148‑4 (MQ=255)
                                                                                                            aTGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTGCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGa                                   >  2:251698/1‑148 (MQ=255)
                                                                                                              gatACTCGCGGGGGTCGTGCTACCACTGGTAAACATGTACTTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                                 <  1:934387/146‑1 (MQ=255)
                                                                                                              gCTACTCTCGGTGGTCGCCGTACCATTAATAAAAACATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                                 >  2:1360233/1‑148 (MQ=255)
                                                                                                              gCTACTCACGGTGGTAGTGATAAAACTGGTAAAAATGTACATGTTGGGGCTGGCGCAGTATTAGCAGGTGTGATTGATGCCCCTAGTGCTTCACCGGTTATAATAGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                                 <  2:1056264/148‑1 (MQ=255)
                                                                                                               cTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAgg                                >  1:1149075/1‑148 (MQ=255)
                                                                                                                    ctcGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTAc                           >  1:1376223/1‑148 (MQ=255)
                                                                                                                         tggtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtg                      >  1:616188/1‑147 (MQ=255)
                                                                                                                           gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCTCCTAGTGCTTCACCGGTTATAATAGAGGATGATGTATTAATCTGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg                    <  2:682586/148‑1 (MQ=255)
                                                                                                                           gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg                    <  2:179423/148‑1 (MQ=255)
                                                                                                                           gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg                    <  1:1028025/148‑1 (MQ=255)
                                                                                                                           gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg                    <  1:1238844/148‑1 (MQ=255)
                                                                                                                           gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTgg                    <  2:1164182/148‑1 (MQ=255)
                                                                                                                           gacGTGCTACAACTGGTAAAAATGAACAGGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGttag                    <  2:370597/146‑3 (MQ=255)
                                                                                                                              gTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATTTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgtcgccca                 >  2:961242/1‑142 (MQ=255)
                                                                                                                                   aCAACTGGTAAAAATGTACCTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg            <  1:151284/148‑1 (MQ=255)
                                                                                                                                   aCAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg            <  2:1236822/148‑1 (MQ=255)
                                                                                                                                   aCAACGGGTAAACATGTTCATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTg            <  2:593190/148‑1 (MQ=255)
                                                                                                                                     aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGGTGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt          >  1:1090948/1‑148 (MQ=255)
                                                                                                                                     aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt          >  1:257157/1‑148 (MQ=255)
                                                                                                                                     aaCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt          >  2:38092/1‑148 (MQ=255)
                                                                                                                                        tGGTAAAAATGTACATGTAGGGGCTGGCGAAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATAGATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTCtt       >  1:728382/1‑148 (MQ=255)
                                                                                                                                             aaaaTGTACATGTATGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAAGCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGTTGc  <  1:422781/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGTTGC  >  USA300TCH1516_ALE/1434936‑1435224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑