| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,196,508 | C→A | S338Y (TCT→TAT) | ftsZ → | Cell division protein FtsZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,196,508 | 0 | C | A | 99.1% | 333.6 / NA | 109 | S338Y (TCT→TAT) | ftsZ | Cell division protein FtsZ |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (52/56); total (53/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTC > USA300TCH1516_ALE/1196364‑1196649 | ttAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAg > 1:284423/1‑148 (MQ=255) tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAAt > 2:1383752/1‑148 (MQ=255) atGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCTTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATAATTATAGCAATg > 2:556214/1‑148 (MQ=255) atGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATg > 2:699902/1‑148 (MQ=255) tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGc > 1:714982/1‑148 (MQ=255) tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGc > 2:141179/1‑148 (MQ=255) tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGc > 1:524143/1‑148 (MQ=255) gATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCCCTGGATTCCGCCCAAGCGTAAAGACTTATAGCAATGCa > 1:27763/1‑148 (MQ=255) gATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAGATACTTATAGGAATGCa > 2:727863/1‑148 (MQ=255) aTTTTCGGTACAGTTATTAATCATGAATTACAAGATGAGATTGTTGTAACAGTTATAGCAACTGGTT‑‑TTGATGAGAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCaa > 2:623801/1‑148 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAAc > 1:766611/1‑148 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAAc > 2:1324968/1‑148 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGGAAATATGGTAGCGCTGGATTCGGAACAAGCGTAAATACTTATAGCAATGGAAc > 2:498516/1‑148 (MQ=255) tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACt < 2:1247764/148‑1 (MQ=255) tttCGGTACAGTTATTAAGCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACt < 2:877079/148‑1 (MQ=255) ggTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTTACGGATCCTGC‑‑CTGGTTTGTTGGTGGCGAACCACCATCACATGGTGGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTa < 2:1353820/148‑1 (MQ=255) ggTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTa > 1:1280337/1‑148 (MQ=255) gTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTaa < 1:865655/148‑1 (MQ=255) gTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATg < 1:776685/148‑1 (MQ=255) ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGa < 1:1354477/148‑1 (MQ=255) tattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGaa > 1:278961/1‑148 (MQ=255) tattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGaa > 2:1342646/1‑148 (MQ=255) ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAACAATGCAACTTCTAAAGATGAATc > 1:1109174/1‑148 (MQ=255) ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACGTATAGCAATGCAACTTCTAAAGATGAATc > 2:894803/1‑148 (MQ=255) aaTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAt > 2:986869/1‑148 (MQ=255) aaTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCCGAACAAGCGCAAATACTTCTAGCAATGCAACTTCTAAAGATGaaccct > 1:913921/1‑144 (MQ=255) aTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGCCAAACCAACATCACATGGTCGTAAATCTGGTGGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt > 2:1132934/1‑148 (MQ=255) aTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt > 2:1231665/1‑148 (MQ=255) aTCCTGAATTACAAGATGAGATAGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt > 1:754021/1‑148 (MQ=255) ccTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAttac > 1:313099/1‑146 (MQ=255) ccTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCa > 2:1086959/1‑148 (MQ=255) ccTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCa > 1:1100115/1‑148 (MQ=255) ccTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCa > 1:1099991/1‑148 (MQ=255) aCAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAAt < 1:1213551/148‑1 (MQ=255) aaGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATtc > 1:1403705/1‑148 (MQ=255) aGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATtca > 2:1160540/1‑148 (MQ=255) aGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTACTAAAGATGAATCATTCACTTCAAATtca > 2:1038226/1‑148 (MQ=255) gagaTTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCaa > 1:1123364/1‑148 (MQ=255) aTTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCTTTCACTTCAAATTCATCAAATg < 2:955332/148‑1 (MQ=255) aTTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATg < 2:451870/148‑1 (MQ=255) ttgtAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATtca < 1:1298191/137‑1 (MQ=255) ttgtAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGAAATGCAACTTCTAAAGATGAATCATTCACTTCAAATtca > 2:1298191/1‑137 (MQ=255) aCAGTTATTGCTACTGGTT‑‑TTGATGACAAACCGACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTTAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCCCTTCAAATTCATCAAATGCACAAGCaa < 2:787287/148‑1 (MQ=255) aCAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGCACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACCAGCac > 1:1323962/1‑147 (MQ=255) aCAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATATCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCaa < 2:1110289/148‑1 (MQ=255) cAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc > 2:1182883/1‑148 (MQ=255) cAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc > 2:961696/1‑148 (MQ=255) cAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc > 2:341108/1‑148 (MQ=255) cAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc > 2:324722/1‑148 (MQ=255) aGTTAGTGCAACTGGTT‑‑TTGATGCCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACATCAAATTCATCAAATGCACAAGCAACt < 1:212523/148‑1 (MQ=255) ttGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGAAACTGATAg < 1:1342646/148‑1 (MQ=255) gccaCTGGTT‑‑TTGATTACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGTTTCGGCACAAGCGTAAATTCTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg < 2:500400/145‑1 (MQ=255) ggTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCTCAAGCAACTGATAGTCTAAGTg < 2:956524/148‑1 (MQ=255) ggTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg < 2:474553/148‑1 (MQ=255) tt‑‑ttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaa > 1:1368146/1‑148 (MQ=255) gctgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCAGAAGCAACTGATAGTGTAAGTGAAAGaa < 2:1343309/146‑1 (MQ=255) gatgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGaa < 1:560210/148‑1 (MQ=255) aaCCAACATCACATGGTCGTAACTCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATAc > 1:779335/1‑148 (MQ=255) aaCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGCTGACTCATTCACTTCAAGTTCATCAAATGCACAAGCAACTGATAGTGTAATTTAAAAAACACATat > 1:29352/1‑147 (MQ=255) aaCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGGAAAAGCAACTCATAGTGTAAGGGAAAGAACACATAc > 2:384923/1‑148 (MQ=255) aaCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAAGACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATAc > 1:619098/1‑148 (MQ=255) aCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa < 1:1230993/148‑1 (MQ=255) aCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa < 1:228370/148‑1 (MQ=255) ccAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACaa < 1:653298/148‑1 (MQ=255) ccAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACaa < 2:278961/148‑1 (MQ=255) cAACATCACATGGTCGTAAATCTGGTAGCACTAGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATAAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAAc < 2:785826/148‑1 (MQ=255) aaCATGACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACt < 2:913921/148‑1 (MQ=255) aCATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTa < 1:986869/148‑1 (MQ=255) aCATCAAATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTa < 1:1131772/148‑1 (MQ=255) aTCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTaaa < 2:766611/148‑1 (MQ=255) aTCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTaaa < 1:1324968/148‑1 (MQ=255) tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAAATGAAACATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAACGAACACATACAACTaaaa > 2:1207106/1‑147 (MQ=255) tCACATGGTCGTAAAGCTGGTAGCACTGGACTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaag < 2:77407/148‑1 (MQ=255) aTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACCCATACAACTAAagaaga > 1:1205712/1‑148 (MQ=255) aTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAagaaga > 1:551301/1‑148 (MQ=255) aTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAagaaga > 1:1205747/1‑148 (MQ=255) tGGTCGTAAATCTTGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgat < 1:473924/148‑1 (MQ=255) ggTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAGCACATACAACTAAAGAAgatg < 2:1087769/148‑1 (MQ=255) gTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga < 2:1175682/148‑1 (MQ=255) tAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGCACACGTACAACTAAAGAAGATGATAtt < 1:775254/148‑1 (MQ=255) aTCTGGTAGCACTGGATTCGGAACCAGCGCAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCt > 2:1001686/1‑148 (MQ=255) aTCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCt > 2:938239/1‑148 (MQ=255) tGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGc > 1:711776/1‑148 (MQ=255) gTAGCACTGTATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAACGAGGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGGTAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCtt < 2:584734/148‑1 (MQ=255) gTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCtt < 2:246627/148‑1 (MQ=255) aGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCa > 1:1246276/1‑148 (MQ=255) tGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGa < 2:1108675/148‑1 (MQ=255) tGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGa < 2:902173/148‑1 (MQ=255) tGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGa < 2:902213/148‑1 (MQ=255) tGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATCAATCATTCACTTGAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGa < 2:963803/148‑1 (MQ=255) ggATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTCCATTAGaa < 2:739407/148‑1 (MQ=255) gATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAGGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGaaa < 2:754021/148‑1 (MQ=255) aTTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAAt < 1:699902/148‑1 (MQ=255) aTTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAAt < 1:556214/148‑1 (MQ=255) ttCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATa > 1:1261152/1‑148 (MQ=255) cGGAACCAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATaga < 2:407971/148‑1 (MQ=255) gAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTAAGTTACAGCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAgaga < 1:324722/148‑1 (MQ=255) aGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaa < 2:284423/148‑1 (MQ=255) gTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc < 2:278099/148‑1 (MQ=255) aTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTc > 1:1360906/1‑148 (MQ=255) | TTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTT‑‑TTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTC > USA300TCH1516_ALE/1196364‑1196649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |