Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,416,458	A→T	I216N (ATT→AAT)	oppD_4 ←	Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,416,458	0	A	T	100.0%	291.9 / NA	85	I216N (ATT→AAT)	oppD_4	Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (50/35);  total (50/35)

TCACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGCAA  >  USA300TCH1516_ALE/1416314‑1416602                                                                                                                                                  |                                                                                                                                                 tCACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGt                                                                                                                                               <  2:908164/148‑1 (MQ=255)  cacttattttAACTCTATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTa                                                                                                                                              <  2:909017/142‑1 (MQ=255)    caTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACt                                                                                                                                            >  1:567913/1‑148 (MQ=255)     aTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTg                                                                                                                                           <  1:868691/148‑1 (MQ=255)     aTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTg                                                                                                                                           <  1:868608/148‑1 (MQ=255)     aTGTTTTAACGCAATCATAGTAATCCTTCTGTAATTGGGTACTATATTTAGTGAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTg                                                                                                                                           <  2:707762/148‑1 (MQ=255)              cTCAATCATAGGAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATAttt                                                                                                                                  <  1:221268/148‑1 (MQ=255)               tCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                                 >  1:495369/1‑148 (MQ=255)               tCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                                 >  2:1150294/1‑148 (MQ=255)                 aaTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTAGACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTACAAGATAATTGTACTGACAATATTTCaa                                                                                                                               <  1:787786/148‑1 (MQ=255)                  aTCATAATAATCCCTCTTTAATTTTTTTCTATATTTAACTAGACGTACACTATACGTATGCAAATGCTTATACTTGAAATGATTAATATTACATCGTTGTATGATATGACCTTC‑TTTTTAAACATAATTGTACTGACGATATTTCAAt                                                                                                                              >  2:160880/1‑148 (MQ=255)                          aaTCCCTCGTTAATTGTTTTATATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                                      <  2:113731/148‑1 (MQ=255)                                tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGCTGCAAATGCTCATACTTGAAATGCTTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAAAATTTCAATACATGACTTAAGtt                                                                                                                >  2:53475/1‑148 (MQ=255)                                tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                                >  1:144211/1‑148 (MQ=255)                                tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                                >  1:727989/1‑148 (MQ=255)                                tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                                >  1:279755/1‑148 (MQ=255)                                tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATAATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATAAATGACTTAAGtt                                                                                                                >  2:1389665/1‑148 (MQ=255)                                     aaTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTAACTCGTTCAATGATTTGACCCTC‑TGTTAAAACTTAATTGTACTGACATTATTTCACTACCCGAATTTAGtttaggg                                                                                                           >  2:1245485/1‑143 (MQ=255)                                      aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                          >  2:214217/1‑148 (MQ=255)                                      aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATAAAGGACTTAAGTTAtgtgt                                                                                                          >  2:281945/1‑148 (MQ=255)                                       ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGCTCTGTTCAACATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                         <  1:670179/148‑1 (MQ=255)                                          tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGata                                                                                                      >  1:1061472/1‑148 (MQ=255)                                          tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTGATGTGGGata                                                                                                      >  1:1060086/1‑148 (MQ=255)                                           gtTCTATATTTGATTAGACGTTCAGTATACGGATGCAAACGCTCATACTTGAACTGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                                     <  1:462511/148‑1 (MQ=255)                                           gtTCTATATTTAATTAGACGTTCAGTATGCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                                     <  1:481653/148‑1 (MQ=255)                                                  atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGTTAataaataa                                                                                              >  1:586186/1‑148 (MQ=255)                                                        ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTCTGTTTGATAATAAATAATGTTTg                                                                                        >  1:765418/1‑148 (MQ=255)                                                        ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                        <  1:221641/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTTAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                      <  2:1326039/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTTATAATAAATAATGTTTGAc                                                                                      <  1:1295652/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                      <  1:519239/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                      <  1:1119087/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                      <  2:899392/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                      <  2:620900/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                      <  2:25001/148‑1 (MQ=255)                                                          aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTGTGAc                                                                                      <  2:1139690/148‑1 (MQ=255)                                                           gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAACTAATGTTTGAcc                                                                                     >  2:1018439/1‑148 (MQ=255)                                                           gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTAAcc                                                                                     >  2:1037750/1‑148 (MQ=255)                                                           gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTAAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                                     >  1:120442/1‑148 (MQ=255)                                                            aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                                    >  1:399630/1‑148 (MQ=255)                                                             cGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                                   <  2:160976/148‑1 (MQ=255)                                                                tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTAAGATCC‑TTTTAAAACACAATTAGACTGACAATATTTCAATACATGACTGAAGTTATGTGTGATAATAAGTAATGTTTGGCCATggt                                                                                >  1:329360/1‑146 (MQ=255)                                                                tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAAGGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                                >  2:920918/1‑148 (MQ=255)                                                                        cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                                                                        <  2:1325253/148‑1 (MQ=255)                                                                         ggATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATACGGTTTGACCATGTTCTAATACaa                                                                       >  1:582631/1‑148 (MQ=255)                                                                            tGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAatat                                                                    <  1:770152/148‑1 (MQ=255)                                                                            tGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATAATAT‑AGTTTCGGCGTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAatat                                                                    <  1:1093524/148‑1 (MQ=255)                                                                                  tGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGta                                                              <  2:1177270/148‑1 (MQ=255)                                                                                    cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATCTGCTGtaat                                                            >  1:381819/1‑148 (MQ=255)                                                                                    cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGgaat                                                            >  1:256387/1‑148 (MQ=255)                                                                                         aCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATc                                                       <  2:567913/148‑1 (MQ=255)                                                                                              aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                                  >  1:793691/1‑148 (MQ=255)                                                                                              aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                                  >  1:728055/1‑148 (MQ=255)                                                                                              aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                                  >  1:905507/1‑148 (MQ=255)                                                                                                    ttAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATCAATCCATCAATTGAt                                            >  1:754447/1‑148 (MQ=255)                                                                                                    ttAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATAAATGACTTAAGTTATGAGTGATAATAAATACTGTTTGACAATGTTCTAGTAAAATATCCTGAAATCAATCCATCACTTgct                                            >  2:737244/1‑146 (MQ=255)                                                                                                       atatTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCGAATACAATATGCTGTAATAAATCCATCACTTGAttgc                                         >  1:711451/1‑146 (MQ=255)                                                                                                         atTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCg                                       <  1:778364/148‑1 (MQ=255)                                                                                                         atTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCg                                       >  1:1375098/1‑148 (MQ=255)                                                                                                         atTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACACTTTTCTAATACAATATGCTGTAATAAATCAATCACTTGATTACCg                                       >  1:955220/1‑148 (MQ=255)                                                                                                           tACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTGTAATCCAATCTGATGTTATAAATCCATCACTTGATTACCGtt                                     >  1:533256/1‑148 (MQ=255)                                                                                                           tACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                                     >  1:1240286/1‑148 (MQ=255)                                                                                                           tACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                                     >  2:1209918/1‑148 (MQ=255)                                                                                                           tACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                                     >  2:576652/1‑148 (MQ=255)                                                                                                           tACCTCGTTAAATGATTTGACATTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTAGACAATTTTATATTGACATAAGAGGTAATAAAGCCATCACATGATTCACGtt                                     >  1:1240366/1‑148 (MQ=255)                                                                                                           tACCTCGGTCAATAATTTGACCTTC‑TTTTAAAACATTATTGTACTGACAATATTTCAAGAATAGACTAAGGTTATGTGTTATTAGAAATAATATTTCACCAATGTCTAATACAATATGCTGTAATAAATACATACCTTGATTACCGtt                                     >  2:356072/1‑148 (MQ=255)                                                                                                            aCCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTc                                    <  2:55108/148‑1 (MQ=255)                                                                                                             ccTCGTTCAATGATTTGACCTTC‑TTTTGAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCa                                   >  1:842427/1‑148 (MQ=255)                                                                                                               tCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                                 >  2:1167189/1‑148 (MQ=255)                                                                                                               tCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGGTCaaa                                 >  2:1166871/1‑148 (MQ=255)                                                                                                               tCGTTCAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCAATCACTTGATTAACGTTaaaa                                 >  1:1043801/1‑148 (MQ=255)                                                                                                                    cAATGATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                            <  2:251614/148‑1 (MQ=255)                                                                                                                        gATTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAat                        >  2:373733/1‑148 (MQ=255)                                                                                                                         aTTTGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATTTGCTGTAATAAATCTATTCCTTGATTACCGTTCAAACAATTCAatg                       >  2:292761/1‑148 (MQ=255)                                                                                                                          tttGACCTTC‑TTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTCTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga                      >  1:938779/1‑148 (MQ=255)                                                                                                                                 ttc‑ttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGATATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCACAGCATCCAATGATGCAACt               >  2:1253868/1‑148 (MQ=255)                                                                                                                                  tc‑ttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCGACTg              >  2:1024226/1‑148 (MQ=255)                                                                                                                                  tc‑ttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg              >  1:416090/1‑148 (MQ=255)                                                                                                                                      tttAAAACATAATTGTACTTACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGtt           <  2:728055/148‑1 (MQ=255)                                                                                                                                      tttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGTTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATGCAATGATGCAACTGGtt           <  2:905507/148‑1 (MQ=255)                                                                                                                                      tttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGtt           <  2:793691/148‑1 (MQ=255)                                                                                                                                          aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTCATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTTAAAGCATCCAATGATGCAACTGGTTCGTc       <  1:731460/148‑1 (MQ=255)                                                                                                                                             cATAATTGAACGTACAATATTTCAATACATGACTTAAATTATGTATGATAATAAATAATGTTTGACCATGTTCTAAAACAAAATGCTGTAATAAATCCATCACTCGATTACCGTTCAAAGCATCCAATGATACAACTGGTTCGTCTGc    <  2:416090/148‑1 (MQ=255)                                                                                                                                               tAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGCaa  <  2:1058361/148‑1 (MQ=255)                                                                                                                                               tAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCAATCACTTGATTACCGTTCAAAGCATCCCATGCTGCAACCGGTTCGTCTGCaa  >  2:1375649/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TCACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTC‑TTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGCAA  >  USA300TCH1516_ALE/1416314‑1416602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑