Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,513,930	A→T	L413F (TTA→TTT)	USA300HOU_RS07375 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,513,930	0	A	T	100.0%	282.6 / NA	87	L413F (TTA→TTT)	USA300HOU_RS07375	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (40/47);  total (40/47)

AATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATT  >  USA300TCH1516_ALE/1513786‑1514073                                                                                                                                                 |                                                                                                                                                aaTCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTAtt                                                                                                                                              <  2:1103597/148‑1 (MQ=255)    cagtAATGTATTTGTATGTAGCGCGTTATTAGTAGCCATTGGCATGGGGTTTGTCTCATGTATAAAGCATACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTa                                                                                                                                           <  2:247633/145‑1 (MQ=255)    cATTAATTTATTTTTAGGTAGCGCTTGATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTa                                                                                                                                           <  2:247568/148‑1 (MQ=255)    cATCAATTTATTTTTATGTCTTGCTTTATGAGGAGCCAATGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTATAAAAAAAAAAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTa                                                                                                                                           <  2:278339/148‑1 (MQ=255)      tttctttatttGTTTTTATTGTTTGATGAGTAGCAAATGGCATGAGGTTTGTCTCTTAGATAAAGAGAACAAAAAAGACTGCAATTATAGTATAAAAAAAAAAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                                         <  2:343823/144‑1 (MQ=255)      ttAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                                         <  1:435690/148‑1 (MQ=255)      taaaTTTATTAGTGTGTTTCGCTTTATGAGTAGCAATGGGCACGGGTTTTGTCCCGTGGATAAAGAGTACAAAAAATACTGCAATTAAAGTACAAAAAAAAAAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                                         <  2:1404961/146‑1 (MQ=255)       tAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTa                                                                                                                                        >  1:1212970/1‑148 (MQ=255)       tAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTa                                                                                                                                        >  1:246259/1‑148 (MQ=255)        aaTTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTAGAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATTTATATCACTAATAATATATTCTATTTTATTAg                                                                                                                                       >  2:786914/1‑148 (MQ=255)        aaTTTATTTGGATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAGGCGTACAAAAAATACTGCAATAAAAGTACAGCATCGCGTAAAAAAACCAGCATCTATATCACACATAATATGGTTTATTATATTAg                                                                                                                                       >  2:588740/1‑148 (MQ=255)         atttatttGTATGTATCGCTTTATAAGAAGCCATTGGCATGGGGTTAGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTCTTAgg                                                                                                                                      >  1:785820/1‑148 (MQ=255)          tttatttGTATGTTTCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTTGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGa                                                                                                                                     <  1:322557/148‑1 (MQ=255)           ttatttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGat                                                                                                                                    >  1:300937/1‑148 (MQ=255)           ttatttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGat                                                                                                                                    >  1:566849/1‑148 (MQ=255)              tttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGattat                                                                                                                                 >  1:48015/1‑148 (MQ=255)              tttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGattat                                                                                                                                 <  1:529049/148‑1 (MQ=255)              tttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGGTTTATTGTATTAGGattat                                                                                                                                 >  2:1383757/1‑148 (MQ=255)              tttGTATGTATCGCTTTATTAGTAGACATCGGCATGGGGTTTGTCTCTTGGGTAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAGAACAAGCATCTATATCACTCACAATATGTTTTATTGTATTAGGattat                                                                                                                                 >  2:915791/1‑148 (MQ=255)              tttGTATGTATCGCTTTAATAGGAGCAATTGGCATGGGGTTTGTCTCTTAGATAAAGCATGCAAACAATACTGCAATAAAAGTACCAAAAAAAATAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGattat                                                                                                                                 <  2:903964/148‑1 (MQ=255)                  tatgtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAAt                                                                                                                             >  1:684848/1‑148 (MQ=255)                     gtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGcc                                                                                                                          >  1:268016/1‑148 (MQ=255)                     gtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTGGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGTATTATTAATGcc                                                                                                                          <  2:165402/148‑1 (MQ=255)                       atCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCat                                                                                                                        >  2:34964/1‑148 (MQ=255)                          gCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCattat                                                                                                                     <  2:176855/148‑1 (MQ=255)                           cTTTTTTAGTAGCCATTGGCATGGGGTTTGTCTCTTTGATAAAGCGTACAAAAAATACTGCAATATAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCattatt                                                                                                                    <  2:1162059/148‑1 (MQ=255)                           cTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGAGAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATAACACGCATAATATGTTTTATTGTATTAGGATTATTAATGCCattatt                                                                                                                    <  2:926111/148‑1 (MQ=255)                            tttATTAGTAGCCATTGGAATGGGGTTTGAATCTTGGATCAAGTGTATAGAAAATTCTTCAATAAAAGTACTACAACGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTAttt                                                                                                                   >  2:278018/1‑148 (MQ=255)                            tttATGAGTAGCCATTGGCATCGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATTTCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTAttt                                                                                                                   <  1:691679/148‑1 (MQ=255)                             ttattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTTCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATCGTATTAGGATTAATAATGCCATTATTTg                                                                                                                  <  1:884225/148‑1 (MQ=255)                               attaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGa                                                                                                                <  1:62270/148‑1 (MQ=255)                                    tagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAACAACCAGCATCTATATCACTATTAATGTGTTTTATTGTATTAGGATTATTAATGCACTTATTTGGATTAtt                                                                                                           >  1:108841/1‑147 (MQ=255)                                    tagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCGTAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATc                                                                                                           >  2:923275/1‑148 (MQ=255)                                    tagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTGGGATTATTAATGCCATTATTTGGATTAtt                                                                                                           >  2:1311733/1‑147 (MQ=255)                                      gCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCAc                                                                                                         <  1:4338/148‑1 (MQ=255)                                                  gggTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTAt                                                                                             <  1:1287438/148‑1 (MQ=255)                                                  gggTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTAt                                                                                             <  1:741961/148‑1 (MQ=255)                                                  gggTTTGTCTCCTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTAt                                                                                             <  1:9344/148‑1 (MQ=255)                                                    gTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGCTTATTGATTCCATTATTTGGATTATCACTTATCCTTGTAttt                                                                                           <  2:1293645/148‑1 (MQ=255)                                                        gTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                                       <  2:740435/148‑1 (MQ=255)                                                        gTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                                       <  2:307359/148‑1 (MQ=255)                                                        gTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGCATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                                       <  2:925618/148‑1 (MQ=255)                                                         tctcTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAAt                                                                                      <  1:1293056/148‑1 (MQ=255)                                                            ctTGGATAAAGCGTACAAAAAAGACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGa                                                                                   <  1:378393/148‑1 (MQ=255)                                                             ttGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                                  <  1:1176714/148‑1 (MQ=255)                                                                 agaaaGGGTACAAATAACACATCAATAAAAGTATCAAATCGCGAAGAAAACCCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGAGTATTAAGGCCATTATTTGGATTATCTCTTGTCCTGTTATTTATAATTCAATTaa                                                                              <  2:1367199/146‑1 (MQ=255)                                                                 aTAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTaa                                                                              <  1:93942/148‑1 (MQ=255)                                                                   aaaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATATATAATTGAATTAgta                                                                            >  2:946200/1‑148 (MQ=255)                                                                    aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATCATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAatat                                                                           <  2:232969/148‑1 (MQ=255)                                                                     aGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAtctt                                                                          >  2:1175799/1‑148 (MQ=255)                                                                     aGCGTACAAAAAATACTGCAACAAAAGTACCACATCCCGTAAAAAAACCAGCATCTCCATCACTTATATTATGTCTTATTGTTTTAGTTTTATTCATGCCAATATTTGGATTATCACTAATCTATGTATTTATAATTGAATTCAtttt                                                                          >  2:1175767/1‑148 (MQ=255)                                                                       cGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAtattat                                                                        <  2:48015/148‑1 (MQ=255)                                                                       cGTACAAAAAATACTGCAATAAAACTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTTTAATTGAATTATttttat                                                                        >  1:702101/1‑148 (MQ=255)                                                                           cAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTatatat                                                                    >  1:1006385/1‑148 (MQ=255)                                                                               aaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTaaa                                                                <  2:1405946/148‑1 (MQ=255)                                                                                  tACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTACCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAt                                                             <  1:1171671/148‑1 (MQ=255)                                                                                      gCAATAAAAGTACCACATCGCGTAGAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAtcgtc                                                         >  1:839094/1‑148 (MQ=255)                                                                                      gCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTGTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATTTATTAAATatcatc                                                         >  1:156113/1‑148 (MQ=255)                                                                                         agaaaaGTACAAAATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                                      <  2:464169/146‑1 (MQ=255)                                                                                         aTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCCTAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGCTTATCACTTCTCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                                      <  2:400795/148‑1 (MQ=255)                                                                                         aTAAAAAGAGCACAGCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                                      <  2:401424/148‑1 (MQ=255)                                                                                             aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTaa                                                  <  2:58359/148‑1 (MQ=255)                                                                                             aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTaa                                                  >  2:57132/1‑148 (MQ=255)                                                                                                tACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACa                                               <  1:1205502/148‑1 (MQ=255)                                                                                                tACCAAATCGCGTAAAAAAACCAGCATTTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACa                                               <  2:72552/148‑1 (MQ=255)                                                                                                 cacataacgcgTATAAAACTCTTCATCTATATCACTCATTTTATGTTTTATTTTTTTAGAATTGTTAATGCCGTCACTTGTATTATCAGCTATCCTTGTTTTTATAATTTAATTAATATTATATATTAAAGATTGTTGTGCTAATCaa                                              <  2:569956/141‑1 (MQ=255)                                                                                                 aCCACATCGCGAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACaa                                              <  2:490528/148‑1 (MQ=255)                                                                                                       tCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGc                                        >  1:903587/1‑148 (MQ=255)                                                                                                       tCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGc                                        >  1:719065/1‑148 (MQ=255)                                                                                                       tCGCGTAAAAAAACCAGCATCTATATCACCCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAAGAATGc                                        <  2:1295696/148‑1 (MQ=255)                                                                                                                          tCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGa                     <  2:1150359/148‑1 (MQ=255)                                                                                                                                ttaCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAACATAATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAt               <  1:967336/146‑1 (MQ=255)                                                                                                                                tCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAATGTTTTGGACTGACGAAAt               >  2:1102688/1‑148 (MQ=255)                                                                                                                                tCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAt               >  2:109614/1‑148 (MQ=255)                                                                                                                                tCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAt               >  2:71967/1‑148 (MQ=255)                                                                                                                                tCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAt               >  1:770979/1‑148 (MQ=255)                                                                                                                                 cACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAtt              >  2:761794/1‑148 (MQ=255)                                                                                                                                 cACGCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAtt              <  1:146507/148‑1 (MQ=255)                                                                                                                                  aCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAttt             >  1:510415/1‑148 (MQ=255)                                                                                                                                  aCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCATTGTAGTTATCCTTTAAGTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACTAAAttt             >  1:1391547/1‑148 (MQ=255)                                                                                                                                      ttaataTGTTTTATTGGAGTAGGATTAGTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAAGAATGCACTTAAAGTTTTGAACTGACGAAATTTACaa         <  1:278018/147‑1 (MQ=255)                                                                                                                                         atatGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAAt      >  1:1237486/1‑148 (MQ=255)                                                                                                                                         atatGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCAATTAAAGTTTTGAACTGACGTAATTAACAAAAt      >  2:879501/1‑148 (MQ=255)                                                                                                                                          tatGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATg     >  1:746714/1‑148 (MQ=255)                                                                                                                                            tGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGAt   >  1:202822/1‑148 (MQ=255)                                                                                                                                             gTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGCACTGACGAAATTTACAAAATGAtt  >  1:947034/1‑148 (MQ=255)                                                                                                                                             gTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGAtt  >  2:1246326/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                AATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATT  >  USA300TCH1516_ALE/1513786‑1514073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑