Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	97.7%	272.1 / ‑6.0	85	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (31/52);  minor base T (0/2);  total (31/54)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCT  >  USA300TCH1516_ALE/1646045‑1646328                                                                                                                                                 |                                                                                                                                            tCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                          >  2:968899/1‑148 (MQ=255)    ataatTTTATCACTTGCAACACCATGTGTTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                       >  1:351175/1‑148 (MQ=255)     taatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                      <  2:10497/148‑1 (MQ=255)       atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacag                                                                                                                                    >  1:282887/1‑147 (MQ=255)        ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                   <  2:830394/148‑1 (MQ=255)        ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                   <  1:82143/148‑1 (MQ=255)        ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                   <  2:566057/148‑1 (MQ=255)        tttgATCACTTGCAACACCATGTAGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                   <  2:1148360/148‑1 (MQ=255)         tttATCACTTGCAACACCCTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                                  <  1:936412/148‑1 (MQ=255)           tATCCCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGCTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTGGCTTTCTAATATTGCCTTTAATTCTCTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                <  1:991793/148‑1 (MQ=255)           tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACATTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                <  2:934479/148‑1 (MQ=255)           tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaacatt                                                                                                                                >  2:215319/1‑146 (MQ=255)            ctCACTAGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTACTTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                               <  2:782515/147‑1 (MQ=255)            aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCACTTTGCTTTCTAATACTGAATTTAATACTTTAACAGTATTGCCTTCTTTTACAACAGtt                                                                                                                               <  2:1036328/148‑1 (MQ=255)              cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                             >  2:739609/1‑148 (MQ=255)               aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTTATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                            <  1:1255138/148‑1 (MQ=255)                  tGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTTTTTTTTTCTTCCTTTTACAATAGTTAAAatg                                                                                                                         >  2:646999/1‑147 (MQ=255)                   gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                                                                        <  2:510752/148‑1 (MQ=255)                         ccgtgttgtAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                                  <  2:61655/145‑1 (MQ=255)                         ccATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                                  <  2:797487/148‑1 (MQ=255)                          cacgtgGTAATGCATCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                                 <  2:1271066/145‑1 (MQ=255)                              gggTAATGCACCTCTATGACCAGATGATACAGTCGTATCGAATAATGGTGCATCTGCTCCTAATTCTAGCAGTTTGCTTTCTAGTATTGCCTTTAATTCTTTTTCAGTCATGCATCCCTTTCCAACAGTTAAAATATATTCATATGtt                                                                                                             >  2:445961/2‑148 (MQ=255)                               ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGttt                                                                                                            <  1:72072/148‑1 (MQ=255)                                      taCCTCTATGACCAGATGCTACAATCCTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaac                                                                                                     <  2:1271477/147‑1 (MQ=255)                                      gagctccATGACCATATGCTACTATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTGTGCTTTCTAATATTGTCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaac                                                                                                     <  2:1270764/145‑1 (MQ=255)                                          tttATTACCTGATGCTACTATCTGATCGTAGGATGGTCTATCTGCTTCGATTTTGAGCCTTTTGCTGTCTATTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAata                                                                                                 <  2:1100218/146‑1 (MQ=255)                                           cttgctccAGATGCTGCATTCGTATCGCATGTTGGTCCATCTGAGCCTAATTCTGGCACTTTGCTTTCTAATATTGCCTTTAATTCTTTTGCAGTCCTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAatat                                                                                                <  2:173539/142‑1 (MQ=255)                                           ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAatat                                                                                                <  1:172586/148‑1 (MQ=255)                                               gACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                            >  1:532148/1‑148 (MQ=255)                                                aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATGTTCATATGTTTCATCAACAATATTAgct                                                                                           >  1:922902/1‑148 (MQ=255)                                                  cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                                                                         >  1:548933/1‑148 (MQ=255)                                                  cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                                                                         >  1:548904/1‑148 (MQ=255)                                                   ataTGCTACAATCGTATCGAATGATCGTCCATCTGCTCCTAATTCTATCATTTTGCTGTCTAATTTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                        <  1:231937/146‑1 (MQ=255)                                                       gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttttt                                                                                    <  1:968899/148‑1 (MQ=255)                                                       gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttttt                                                                                    <  1:739609/148‑1 (MQ=255)                                                           cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                                <  2:532148/148‑1 (MQ=255)                                                             aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAATCATATGTTTCATCAACACTATTAGCTCCTTTTTAAATTa                                                                              >  2:397554/1‑148 (MQ=255)                                                             aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCAAGCATATTGCTTTCTAATATGGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTa                                                                              <  2:989439/148‑1 (MQ=255)                                                              tCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGTCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                             <  2:927066/148‑1 (MQ=255)                                                              tCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                             <  2:479522/148‑1 (MQ=255)                                                              tCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                             <  1:633405/148‑1 (MQ=255)                                                                     gAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                      >  1:883323/1‑148 (MQ=255)                                                                       atgatgGTCCATCTGCTCCTAATTCTAGGATTTTGCTTTCTAATATTGCCTTCAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                    >  1:858696/1‑148 (MQ=255)                                                                       atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTCATTCTTTTTCAGTCATGCCTCCATCTACAACAGTTAAAATATATTCATCAGTGTCATCAACAATATTAGCTGCTTTTTGAATTCAAGCattgtg                                                                    >  2:205481/1‑143 (MQ=255)                                                                           tgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCa                                                                <  1:758951/148‑1 (MQ=255)                                                                            ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACATTATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAg                                                               <  1:908358/148‑1 (MQ=255)                                                                                        ccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                                   <  1:990185/148‑1 (MQ=255)                                                                                           aaTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                <  2:893611/148‑1 (MQ=255)                                                                                           aaTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                <  2:1403000/148‑1 (MQ=255)                                                                                           aaTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                <  1:1096579/148‑1 (MQ=255)                                                                                           aaTGCTCGCATTTTGCTTTCTGATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCAGCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                <  2:488895/148‑1 (MQ=255)                                                                                            aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                               <  1:126510/148‑1 (MQ=255)                                                                                             ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                              <  1:442420/148‑1 (MQ=255)                                                                                              tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                             >  2:594481/1‑148 (MQ=255)                                                                                                tAGCATTTTGCTTTCTAATATTGCCTGTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACACTATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCCGCATCTTTGCCGTCTCTACtt                                           >  2:1324575/1‑148 (MQ=255)                                                                                                tAGCATTTTGCTTACTAATATAGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                           >  2:1324349/1‑148 (MQ=255)                                                                                                  gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                         >  1:1208964/1‑148 (MQ=255)                                                                                                            ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCATTTTACAACAGTTAAAATATATTTATATTTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCACTTTCGTCAGATTCTTTGACGTCTTTAATTTTATCTaatgta                               >  2:355320/1‑143 (MQ=255)                                                                                                            ttCTAATATTGCCTTTAACTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTAAGCATCTTTGACGTCTCTAATTTTATCTCCAGTa                               >  2:953898/1‑148 (MQ=255)                                                                                                               tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCGAACTTTATCTCCCGTacgt                            <  2:1006863/148‑4 (MQ=255)                                                                                                                 atatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                          >  1:1104129/1‑148 (MQ=255)                                                                                                                  tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTCCGCGGTTTTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                         <  1:1093156/148‑1 (MQ=255)                                                                                                                  tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                         <  2:547309/148‑1 (MQ=255)                                                                                                                  tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTAACAACACATAAAATATATTCATATGTTTCATCAACAATACTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                         <  2:843470/148‑1 (MQ=255)                                                                                                                     tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACTTCTCTAATTTTATCTACAGTATTAGAAATg                      <  2:1029760/148‑1 (MQ=255)                                                                                                                     tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                      <  2:904529/148‑1 (MQ=255)                                                                                                                     tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                      <  2:235354/148‑1 (MQ=255)                                                                                                                      gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                     <  1:564236/148‑1 (MQ=255)                                                                                                                       tcTTTTATTTTTTTTCAGTGATTGCTCTTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTTGCTGCTTTTTTAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                    <  2:901781/147‑1 (MQ=255)                                                                                                                       cgtttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                    <  1:1004103/146‑1 (MQ=255)                                                                                                                       ccTTTAATTGTTTTTCAGTCATGCCTCCTTTTACAACAGTTACAATATAGTCATATGGTTCATCAACACTATTAGCTGCTTTATGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                    >  2:1287625/1‑148 (MQ=255)                                                                                                                       ccTTTAATTCTTTTTCAGTCATGCCTCCTGTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                    <  2:890550/148‑1 (MQ=255)                                                                                                                        cTTTATTTCTTCTGCAATCTTGCCTTGTGGTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                   <  2:351175/148‑1 (MQ=255)                                                                                                                          ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat                 >  1:190012/1‑148 (MQ=255)                                                                                                                                cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCATTTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa           <  2:858696/148‑1 (MQ=255)                                                                                                                                 tttttCTGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat          <  1:665964/148‑1 (MQ=255)                                                                                                                                 tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat          >  2:554324/1‑148 (MQ=255)                                                                                                                                  ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGtta         >  1:352991/1‑148 (MQ=255)                                                                                                                                  ttttCAATCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata         >  2:1221841/1‑148 (MQ=255)                                                                                                                                  tttcCAGTCATGCCGCCTGTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTCCGTCAGCCTCTTTGACGTCTCTAATTTTATCCACAGTATTAGAAATGCTTATTAATGata         <  1:857772/148‑1 (MQ=255)                                                                                                                                     tCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACCATGTTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGACTCTAAGTTTATCTACAGTATTACAAATGCTTATTAATGATATAc      >  2:288629/1‑148 (MQ=255)                                                                                                                                         tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCt  >  1:231730/1‑148 (MQ=255)                                                                                                                                         tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCt  <  1:87650/148‑1 (MQ=255)                                                                                                                                         tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCt  >  1:36188/1‑148 (MQ=255)                                                                                                                                         tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAAAATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCt  >  1:1328854/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                            TCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCT  >  USA300TCH1516_ALE/1646045‑1646328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑