BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,343,438	C→T	intergenic (+114/+250)	USA300HOU_RS11955 → / ← aldC_1	65 kDa membrane protein/Alpha‑acetolactate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,343,438	0	C	T	100.0%	407.5 / NA	124	intergenic (+114/+250)	USA300HOU_RS11955/aldC_1	65 kDa membrane protein/Alpha‑acetolactate decarboxylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (56/68); total (56/68)

Only 100 of 124 total aligned reads displayed.

AATCAAACAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTATAAAT  >  USA300TCH1516_ALE/2343294‑2343580

aaTCAAACAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAAt                                                                                                                                             <  1:934815/148‑1 (MQ=255)

aaTCAAACAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAAt                                                                                                                                             <  1:135132/148‑1 (MQ=255)

   cAAACAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGt                                                                                                                                          >  2:1234442/1‑148 (MQ=255)

     aaCAGGTACAAATTGNAGTTAAATAAAAATTTGAAAATATTTTTATAGCGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTaa                                                                                                                                        <  1:1116563/148‑1 (MQ=255)

     aaCAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTaa                                                                                                                                        <  2:1375103/148‑1 (MQ=255)

     aaCAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTaa                                                                                                                                        <  2:461289/148‑1 (MQ=255)

        aGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAtt                                                                                                                                     <  1:122753/148‑1 (MQ=255)

        aGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAtt                                                                                                                                     <  1:1280656/148‑1 (MQ=255)

          aTACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAtttt                                                                                                                                   <  1:1129002/148‑1 (MQ=255)

          aTACAAATTGGAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAtttt                                                                                                                                   <  1:589577/148‑1 (MQ=255)

           tACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGGTGTACAATTGTCTTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTta                                                                                                                                  <  1:323625/148‑1 (MQ=255)

               aaTTGTAGTAAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATg                                                                                                                              >  1:203076/1‑148 (MQ=255)

                    tAGTTAAATAATAAGTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgt                                                                                                                         <  1:1367768/148‑1 (MQ=255)

                     aGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgtg                                                                                                                        >  1:1040152/1‑148 (MQ=255)

                     aGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgtg                                                                                                                        >  2:1240112/1‑148 (MQ=255)

                     aGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgtg                                                                                                                        >  2:12579/1‑148 (MQ=255)

                     aGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgtg                                                                                                                        >  1:1063625/1‑148 (MQ=255)

                     aGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGGTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgtg                                                                                                                        >  2:1211978/1‑148 (MQ=255)

                       ttAAATAATCATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGACGTTTGCCTTTTTAGTTCTTGAGCTGGTCAATTATTGTTGTCTGCTTATCGTAGATACAGATTGGCCTATCTAACAGTAAATTTTATATGAGTGTGTa                                                                                                                      <  1:1053165/148‑1 (MQ=255)

                       ttAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGGAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTa                                                                                                                      <  1:156879/148‑1 (MQ=255)

                        tAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTAc                                                                                                                     <  2:547170/148‑1 (MQ=255)

                          aataataatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTC‑TTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTCAATTTTATATGAGTGTGTACcac                                                                                                                  <  2:825446/148‑1 (MQ=255)

                           ataatattTTGAAAAGAGTTTTATAGGGCAAGCGATGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACcac                                                                                                                  <  2:1138793/148‑1 (MQ=255)

                           ataataatTTGAAAATAGTTTTAGAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACcac                                                                                                                  <  2:222419/148‑1 (MQ=255)

                            taataatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACcaca                                                                                                                 <  2:545197/148‑1 (MQ=255)

                             aataatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACaa                                                                                                                <  1:1033334/148‑1 (MQ=255)

                              ttaatGTGAAAAGACTTTTATAGGGCAAGCGCTGAAAATCTGGTGTTTGTCTGTTTAGTTATTGCACCTGTTAATTATTGTTGTTTGCTTCTCGTAGTAACCGATTGGCCTATATAATAGTCAATTTTATATGAGTGTGTACCACAAt                                                                                                               <  2:489434/147‑1 (MQ=255)

                              ataatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTATCAGAAt                                                                                                               <  2:1146571/148‑1 (MQ=255)

                                aatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCGATATAATAGTAAATTTTATATGAGTGTGTACCACAAttt                                                                                                             <  1:547346/148‑1 (MQ=255)

                                 atgtGAAAAGAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTAGTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                                                                            <  2:1149457/145‑1 (MQ=255)

                                 atTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                                                                            >  1:1223673/1‑148 (MQ=255)

                                 atTTGAAAAGAGTTTTATAGGGCAAGCTCATAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                                                                            <  1:1132558/148‑1 (MQ=255)

                                  tttGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTtat                                                                                                           >  1:927738/1‑148 (MQ=255)

                                    tGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATaa                                                                                                         >  1:1361571/1‑148 (MQ=255)

                                    tGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAACTTATaa                                                                                                         >  2:181333/1‑148 (MQ=255)

                                     gAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTTTACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATTAGTGTGTACCACAATTTATAAt                                                                                                        >  2:574515/1‑148 (MQ=255)

                                       taaTTGTGTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACGTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCt                                                                                                      >  2:1072511/2‑148 (MQ=255)

                                       aaaTAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCt                                                                                                      <  1:348798/148‑1 (MQ=255)

                                             ttttATAGGGCAAGCGCTGAAAAATTGGCGGTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGGGTGTATACCACAATTTATAATCTAAACAt                                                                                                >  1:243910/1‑148 (MQ=255)

                                              tttATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATa                                                                                               >  1:614364/1‑148 (MQ=255)

                                              tttATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATa                                                                                               >  2:832974/1‑148 (MQ=255)

                                                tataGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAAc                                                                                             >  1:480640/1‑148 (MQ=255)

                                                 ataGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAAGATAGCa                                                                                            >  1:699074/1‑148 (MQ=255)

                                                 ataGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                                                                            >  1:495971/1‑148 (MQ=255)

                                                    gggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCa                                                                                         >  1:389856/1‑148 (MQ=255)

                                                     ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTACACATAACAGCaa                                                                                        >  2:424519/1‑148 (MQ=255)

                                                     ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaa                                                                                        >  2:1170081/1‑148 (MQ=255)

                                                     ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaa                                                                                        >  2:401704/1‑148 (MQ=255)

                                                     ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAATAACAGATTGGCCTATATAATAGTGAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaa                                                                                        >  2:314615/1‑148 (MQ=255)

                                                     ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTAGTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaa                                                                                        >  1:824555/1‑148 (MQ=255)

                                                     ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTAATCCACTTCTAACTTCTCCATCTCTGAATCGCGTAGCAACAAATTGGCGTAAATAATAGCAAATTTTATATGAGTGTGTGCCGGAATTTATAATCGAAACAGAACAGcca                                                                                        >  2:471016/1‑146 (MQ=255)

                                                      gCAAGCGCTGAAAACTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaaa                                                                                       >  2:779808/1‑148 (MQ=255)

                                                      gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaaa                                                                                       >  2:541271/1‑148 (MQ=255)

                                                      gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaaa                                                                                       >  1:173918/1‑148 (MQ=255)

                                                      gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATGGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaac                                                                                       >  2:502204/1‑147 (MQ=255)

                                                        aaGCGCTGAAAAATTGGCTTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtc                                                                                     <  2:205091/148‑1 (MQ=255)

                                                         aGCGCTGAAAAATTGGCTTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCATCACATAGTAGCATATTGGACCAAATAATATTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtct                                                                                    <  1:330099/148‑1 (MQ=255)

                                                          gcgcTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtctc                                                                                   <  1:432340/148‑1 (MQ=255)

                                                              tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAat                                                                               >  1:340499/1‑148 (MQ=255)

                                                                 aaaaTTGGTGTTTGTTTTTTTACTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGGAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                                                                            <  2:1322000/148‑1 (MQ=255)

                                                                 aaaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                                                                            <  2:1033388/148‑1 (MQ=255)

                                                                  aaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTTTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCt                                                                           <  2:185352/148‑1 (MQ=255)

                                                                   aaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTg                                                                          <  1:69709/148‑1 (MQ=255)

                                                                        gCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAAcc                                                                     <  1:396372/148‑1 (MQ=255)

                                                                                      atttATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGACTGGCCTATATAATAGCAAATTTCATAATACTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTc                                                       <  2:1207385/146‑1 (MQ=255)

                                                                                        ttATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCac                                                     >  2:1102460/1‑148 (MQ=255)

                                                                                         tATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCact                                                    >  1:1056901/1‑148 (MQ=255)

                                                                                           ttGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCactac                                                  >  2:282141/1‑148 (MQ=255)

                                                                                            tGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAGCCTAATAAATCCCCTCactact                                                 <  1:738816/148‑1 (MQ=255)

                                                                                            tGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCactact                                                 <  1:1178847/148‑1 (MQ=255)

                                                                                            tGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCactact                                                 >  1:1277719/1‑148 (MQ=255)

                                                                                             gAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTg                                                <  1:51488/148‑1 (MQ=255)

                                                                                              aaCTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCAACTCACTACTGc                                               >  1:767467/1‑148 (MQ=255)

                                                                                                  tGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTCCTGCTAAt                                           >  2:1241663/1‑148 (MQ=255)

                                                                                                             gttgttTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGat                                >  2:1172718/1‑148 (MQ=255)

                                                                                                               tgttTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGattt                              >  2:420806/1‑148 (MQ=255)

                                                                                                               tgttTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGattt                              >  1:1355730/1‑148 (MQ=255)

                                                                                                               tgttTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGGACCACACGTTATAATCTAAACATAACAGCAACTCTCAAATCACTGGAACCTAATAAATCCCCTCACTACGGCTAATAGAGAAGGGattt                              >  1:323501/1‑148 (MQ=255)

                                                                                                                  ttGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGCAGGGatttatt                           >  1:480242/1‑148 (MQ=255)

                                                                                                                  ttGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttatt                           >  1:756169/1‑148 (MQ=255)

                                                                                                                   tGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttattt                          <  2:388118/148‑1 (MQ=255)

                                                                                                                          cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACt                   <  2:173918/148‑1 (MQ=255)

                                                                                                                            tattaACAGATTGGCCTATATAAGAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTa                 <  1:654479/145‑1 (MQ=255)

                                                                                                                            tagtaACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTTCTAATAGTGAAGGGATTTATTTTATTACTTa                 >  1:459350/1‑148 (MQ=255)

                                                                                                                            tagtaACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTa                 <  1:208090/148‑1 (MQ=255)

                                                                                                                            tagtaACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTa                 <  1:1089027/148‑1 (MQ=255)

                                                                                                                            tagtaACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTa                 >  1:587665/1‑148 (MQ=255)

                                                                                                                              gtaACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGa               <  1:1211978/148‑1 (MQ=255)

                                                                                                                               tcaCAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATACCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGAc              <  1:332359/146‑1 (MQ=255)

                                                                                                                                aaCAGATTTGCCTATATAATAGTAAATTTTATATGAGTGTGCACCACAATTTATAACCTAAACATAACAGCAAATCTCAAATATCTAGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACg             <  2:651338/148‑1 (MQ=255)

                                                                                                                                aaCAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACg             <  2:1138551/148‑1 (MQ=255)

                                                                                                                                aaCAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACg             <  2:133427/148‑1 (MQ=255)

                                                                                                                                  cAGATTTGCCTATATAATTGGAAATTTTATATGAGTGTTTACCACAATTTATAATCCAAACATAACAGCTAATCTCAAATATCTGGAACCTAATAATTCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTa           <  2:257209/148‑1 (MQ=255)

                                                                                                                                  cAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACATAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTa           <  2:1040152/148‑1 (MQ=255)

                                                                                                                                   aGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTaa          <  1:282141/148‑1 (MQ=255)

                                                                                                                                       tGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATACTTTGAACTTAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATtat      >  1:272734/1‑148 (MQ=255)

                                                                                                                                       tGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATtat      <  2:480640/148‑1 (MQ=255)

                                                                                                                                         gCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATACTCTTAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTATaa    <  1:1240112/148‑1 (MQ=255)

                                                                                                                                         gCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTATaa    <  1:12579/148‑1 (MQ=255)

                                                                                                                                           cTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTATAAat  <  2:1351047/148‑1 (MQ=255)

AATCAAACAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTATAAAT  >  USA300TCH1516_ALE/2343294‑2343580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`