BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	584,121	G→T	intergenic (+205/‑67)	rplA → / → rplJ	50S ribosomal protein L1/50S ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	584,121	0	G	T	100.0%	399.7 / NA	129	intergenic (+205/‑67)	rplA/rplJ	50S ribosomal protein L1/50S ribosomal protein L10
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (80/49); total (80/49)

Only 100 of 129 total aligned reads displayed.

CAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGT  >  USA300TCH1516_ALE/583978‑584264

cAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTggg                                                                                                                                             <  2:985829/148‑1 (MQ=255)

cAATAAGTAATTTCCAAGTTATACTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTggg                                                                                                                                             >  1:1040072/1‑148 (MQ=255)

   tAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCACGCACTTTTTGCCTTGGGTAg                                                                                                                                          >  1:1000122/1‑148 (MQ=255)

   tAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTGATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAg                                                                                                                                          >  2:850549/1‑148 (MQ=255)

    aaGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGATATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGa                                                                                                                                         >  1:883908/1‑148 (MQ=255)

     aGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                        <  1:339430/148‑1 (MQ=255)

      gTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaaa                                                                                                                                       >  1:799429/1‑148 (MQ=255)

         aTTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTg                                                                                                                                    <  2:1246228/148‑1 (MQ=255)

          tttCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGc                                                                                                                                   >  1:154123/1‑148 (MQ=255)

          tttCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGAAAGTAAGAGTTATTTATAAATGAAAATTGAGCCTACCGAGGCTAAAATTGACTTGAACGGGATGATCTAGGATCTTTCAAGCACTTTTTGACTTGGGTAGAAAGTGc                                                                                                                                   >  1:641452/1‑148 (MQ=255)

            tCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtt                                                                                                                                 <  1:1260739/148‑1 (MQ=255)

              cAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtttt                                                                                                                               >  2:254031/1‑148 (MQ=255)

              cAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAACTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtttt                                                                                                                               >  2:1022166/1‑148 (MQ=255)

              cAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTAACCTTCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtttt                                                                                                                               >  2:260008/1‑148 (MQ=255)

               aaGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttttt                                                                                                                              >  1:381507/1‑148 (MQ=255)

               aaGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttttt                                                                                                                              >  1:1368189/1‑148 (MQ=255)

                aGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtttttt                                                                                                                             >  2:592254/1‑148 (MQ=255)

                aGTGATATTACGTAGTGTGATTAGTTTACCTAAGACAGTAGGAGTTATTTGTAGCTTAAAATTTATCCTGCCGAGGCTAAATTTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTGGGGTGGAAAGTGGtttggt                                                                                                                             <  2:307658/148‑4 (MQ=255)

                        tACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGATCGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAtt                                                                                                                     <  2:851419/148‑1 (MQ=255)

                        tACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAtt                                                                                                                     <  2:1301810/148‑1 (MQ=255)

                         aCTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTTGAAAGTGCTTTTTTTATTAAttt                                                                                                                    >  2:811134/1‑148 (MQ=255)

                          cTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCCATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAtttt                                                                                                                   >  1:753170/1‑148 (MQ=255)

                              ttGTGATTATTTTAGCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaa                                                                                                               <  2:142771/148‑1 (MQ=255)

                              tctggATTAATTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTCATGATCTATGATCTTTCAAGCACTTTTTGCTTTGGGTAGAAAGCGCTTTTTTAATAAATATAaaaa                                                                                                               <  2:94101/144‑1 (MQ=255)

                                 tgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaaa                                                                                                            >  1:340319/1‑148 (MQ=255)

                                 tgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaaa                                                                                                            >  1:678394/1‑148 (MQ=255)

                                  gATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAg                                                                                                           >  1:743440/1‑148 (MQ=255)

                                  gATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAg                                                                                                           >  1:475348/1‑148 (MQ=255)

                                    ttattTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAgaa                                                                                                         >  2:815403/1‑146 (MQ=255)

                                      attTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATCGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAcc                                                                                                       >  1:946429/1‑148 (MQ=255)

                                       ttttACCGAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCa                                                                                                      <  2:1342707/148‑1 (MQ=255)

                                        tttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaa                                                                                                     >  1:118359/1‑148 (MQ=255)

                                        tttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaa                                                                                                     >  1:310316/1‑148 (MQ=255)

                                        tttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaa                                                                                                     >  1:1303951/1‑148 (MQ=255)

                                        tttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGGAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaa                                                                                                     >  1:1088420/1‑148 (MQ=255)

                                         ttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaaa                                                                                                    <  1:723710/148‑1 (MQ=255)

                                             cTAAGACTGTATGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTTAAATTGACTTGTACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTTTTAATTTTAAAAAAAGAAAAAAAAAtt                                                                                                <  2:966290/148‑1 (MQ=255)

                                             cTAAGACATTACGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAtt                                                                                                <  2:340511/148‑1 (MQ=255)

                                               aaGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTa                                                                                              <  1:1154975/148‑1 (MQ=255)

                                                 gACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                                            >  2:529895/1‑148 (MQ=255)

                                                 gACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                                            >  2:250779/1‑148 (MQ=255)

                                                 gACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGACGATGCAAATTACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCACAAATTTaaa                                                                                            >  1:331689/1‑148 (MQ=255)

                                                      tAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAg                                                                                       >  1:215216/1‑148 (MQ=255)

                                                          aGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtgt                                                                                   <  1:1346187/148‑1 (MQ=255)

                                                          aGTTATTTATAACTTAAAATTTATCCTACCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtgt                                                                                   >  1:844590/1‑148 (MQ=255)

                                                           gTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTCAATGGAGGTGTc                                                                                  >  1:275543/1‑148 (MQ=255)

                                                            ttatttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCt                                                                                 <  2:901714/148‑1 (MQ=255)

                                                               tttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGaa                                                                              >  1:691345/1‑148 (MQ=255)

                                                               tttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGaa                                                                              >  1:254029/1‑148 (MQ=255)

                                                                   taACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTc                                                                          <  1:805184/148‑1 (MQ=255)

                                                                   taACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTc                                                                          <  1:340088/148‑1 (MQ=255)

                                                                       ttAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTGTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCt                                                                      <  2:1040072/148‑1 (MQ=255)

                                                                          aaaTTTATCCTGCCGAGTCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                                   <  2:567951/148‑1 (MQ=255)

                                                                          aaaTTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                                   >  1:501245/1‑148 (MQ=255)

                                                                             tttATCCTGCCGAGGCTAAAATTGACTTGAACGTGACGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCAtt                                                                <  1:847108/148‑1 (MQ=255)

                                                                                 tCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAg                                                            >  1:849812/1‑148 (MQ=255)

                                                                                 tCCTGCCGAGGCTAAAATTGACTTGAACGAGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGAGTAGAAAGTGCTTTCTTTATTAATTTTAAAAAAAGCACCAAAAATCTGAATGAAGATATCTGAATATATGCAACCATAGAAg                                                            >  1:729269/1‑148 (MQ=255)

                                                                                  ccTGCCGGGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGc                                                           <  1:921034/148‑1 (MQ=255)

                                                                                  ccTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGc                                                           <  1:753778/148‑1 (MQ=255)

                                                                                  ccTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGc                                                           >  1:381653/1‑148 (MQ=255)

                                                                                  ccTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGc                                                           >  2:852202/1‑148 (MQ=255)

                                                                                   cTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                                          >  1:1101468/1‑148 (MQ=255)

                                                                                   cTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACGTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGGAGCt                                                          >  1:676892/1‑148 (MQ=255)

                                                                                     gCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaa                                                        >  1:1373610/1‑148 (MQ=255)

                                                                                       cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaaaa                                                      >  1:1076866/1‑148 (MQ=255)

                                                                                       cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaaaa                                                      >  1:1353184/1‑148 (MQ=255)

                                                                                       cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTAAAGCTcaac                                                      >  1:1202416/1‑147 (MQ=255)

                                                                                         aGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTATTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGGCTGCTATAATTGAAGCTaaaaaa                                                    >  2:153392/1‑148 (MQ=255)

                                                                                                aaTTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAg                                             >  1:431393/1‑148 (MQ=255)

                                                                                                   tGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtg                                          >  2:739114/1‑148 (MQ=255)

                                                                                                      cTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTTTTTTTTTTTTAAAGTGCTTATTTTATTAATTTTAAAAAAAGCACCAAAAAGGTAAATGGAGGTGTCTGCATGTCTGCTATCATTGAAGGTAAAAAACAACTAGTtgatg                                       >  2:1382145/1‑148 (MQ=255)

                                                                                                        tGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAGTATTAAAAAAAGCACCAAAAAATTAGATGGCGGATGCTGGATGTCTGCTATCATTTAAGCTAAAAAACAACTAGTGGATaaa                                     >  2:411802/1‑148 (MQ=255)

                                                                                                         gAACGTGATGATCTATGATCTTTCAAGCACTCTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGaaa                                    <  1:403821/148‑1 (MQ=255)

                                                                                                               gatgatCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTg                              >  1:467534/1‑148 (MQ=255)

                                                                                                               gatgatCTATGATCTTTCAAGCAATTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCGGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTg                              <  2:1172276/148‑1 (MQ=255)

                                                                                                                atgatctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAATTTCTTTTTTTAAAAAAAAAAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGa                             <  2:1291687/148‑1 (MQ=255)

                                                                                                                 tgatctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAg                            <  2:82399/148‑1 (MQ=255)

                                                                                                                 tgatctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAg                            <  2:340319/148‑1 (MQ=255)

                                                                                                                 tgaactatgatatTTCAAGCACTTTTTGCATTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATAAATAAAAAAAAAAAACAACTAGTTGATGAAATTGCTGAg                            <  2:678394/148‑1 (MQ=255)

                                                                                                                   atctattatctTTCAAGCACTTTTTGCCTTGGGTTGAAAGGGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATGTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGt                          <  1:541029/148‑1 (MQ=255)

                                                                                                                   atctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGt                          <  1:763377/148‑1 (MQ=255)

                                                                                                                    tctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTa                         <  2:215216/148‑1 (MQ=255)

                                                                                                                    tctatgatctTTCAAGCACTTTTTGCCTTGGGCAGAAAGTGCTTTTTTTATTGACTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAAGGTCTGCTATCATTGAAGCTAAAATACAACTAGTTGATGAAATTGCTGAGGTa                         <  2:772149/148‑1 (MQ=255)

                                                                                                                    tcgatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTa                         <  2:1042277/145‑1 (MQ=255)

                                                                                                                     ctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTAc                        <  2:135643/148‑1 (MQ=255)

                                                                                                                     ctatgatctTTCAAACACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTAATTAATTTTAAAAAAAGCACCAAAAAGTTAAAGGGAGGTGTCTGAATGTCTGCTATCATTGAAGCGAAAAAACAACTAGTTGATGAAATTGCTGAGGTAc                        <  2:202730/148‑1 (MQ=255)

                                                                                                                        tgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAACTGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAAATAGTTGATGAAATTGCGGAGGGACTAt                     >  1:113937/1‑148 (MQ=255)

                                                                                                                        tgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACAAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTAt                     >  1:641658/1‑148 (MQ=255)

                                                                                                                        tgatctTTCAAGCACTTTTTGCCTGGGATAGAAAGTGCTTTTTTTAGTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTAt                     >  1:785319/1‑148 (MQ=255)

                                                                                                                          atctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCa                   >  2:916754/1‑148 (MQ=255)

                                                                                                                          atctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCa                   >  2:1168542/1‑148 (MQ=255)

                                                                                                                           tctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAACTTTGTTTGGAAGTATGTTTTTTGCTTCTTTCTTAGAAGTGATAAAACAACTAGTTGTTGAATTTTCGGAGTTACTAGCaa                  <  2:641572/148‑1 (MQ=255)

                                                                                                                                 aaGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAg            >  1:628302/1‑148 (MQ=255)

                                                                                                                                  aGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGt           >  1:1056638/1‑148 (MQ=255)

                                                                                                                                  aGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGt           >  1:300433/1‑148 (MQ=255)

                                                                                                                                  aGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGt           >  2:734580/1‑148 (MQ=255)

                                                                                                                                      cTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCa       <  1:33200/148‑1 (MQ=255)

                                                                                                                                        ttttGCTTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAAc     <  1:250779/148‑1 (MQ=255)

                                                                                                                                        ttttGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAAc     <  1:529895/148‑1 (MQ=255)

                                                                                                                                           tGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGt  >  2:913280/1‑148 (MQ=255)

CAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGT  >  USA300TCH1516_ALE/583978‑584264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`