BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	973,213	C→A	E175* (GAG→TAG)	yitU_1 ←	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	973,213	0	C	A	100.0%	336.9 / NA	105	E175* (GAG→TAG)	yitU_1	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (65/40); total (65/40)

Only 100 of 105 total aligned reads displayed.

TATCATTATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGAAAA  >  USA300TCH1516_ALE/973069‑973357

tatCATTATCTTCATAACCGAATGCAATAATATTATTTAGGCTTAGTAATT‑‑TAAATTGACTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAat                                                                                                                                               >  1:676589/1‑148 (MQ=255)

   cATTATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGATGCGCCCCAGCGTCGATGCTAAATATg                                                                                                                                            >  2:421505/1‑148 (MQ=255)

    aTTATCTTCATCACCGAATGCAATACTATTAATTCAG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGa                                                                                                                                           >  1:1400059/1‑148 (MQ=255)

     ttATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAACTTCTCTAACTTGTTCACTGTCCCTTGGTTTATTAATATCAAGTTCTATAATGTCCATGACTGTGAAGGGGACGCCTCAGCGTCGATGCTAAATATGAt                                                                                                                                          >  2:471326/1‑148 (MQ=255)

     ttATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCGAGCGTCGATGCTAAATATGAt                                                                                                                                          >  2:312992/1‑148 (MQ=255)

     ttATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGAt                                                                                                                                          >  1:430844/1‑148 (MQ=255)

     ttATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGAt                                                                                                                                          >  1:153045/1‑148 (MQ=255)

     ttATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGAt                                                                                                                                          >  1:1100067/1‑148 (MQ=255)

     ttATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCACAGCGTCGATGCTAAATATGAt                                                                                                                                          >  2:59033/1‑148 (MQ=255)

      tATCTTCATCACCGAATGCAATCATAGTATTTCGG‑‑AAGTCTCTGCATTCGATTCTAGCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATc                                                                                                                                         <  1:663296/148‑1 (MQ=255)

       aTCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCg                                                                                                                                        >  2:362725/1‑148 (MQ=255)

        tCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCgg                                                                                                                                       >  1:504239/1‑148 (MQ=255)

         cTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGc                                                                                                                                      >  2:104011/1‑148 (MQ=255)

            catccCCCAATGCAATTTCATTATTTCGG‑TCAATACTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATa                                                                                                                                   <  2:744333/148‑1 (MQ=255)

                 ccGAATGAAATAATATAATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCGTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCTCCAGCGTCGATGATAAATATGATAGCCATAAAAAt                                                                                                                              >  2:863808/1‑148 (MQ=255)

                  cGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCGTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAAGTGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATg                                                                                                                             >  2:362662/1‑148 (MQ=255)

                    aaTGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAg                                                                                                                           >  1:327801/1‑148 (MQ=255)

                    aaTGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAg                                                                                                                           >  1:1389848/1‑148 (MQ=255)

                    aaTGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATAAGATAGACATAAAAAAGaa                                                                                                                           >  1:832248/1‑147 (MQ=255)

                     aTGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGt                                                                                                                          >  1:894960/1‑148 (MQ=255)

                         aataatATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGc                                                                                                                      <  2:69847/148‑1 (MQ=255)

                          ataataTTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCa                                                                                                                     >  2:1124217/1‑148 (MQ=255)

                          ataataTTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCa                                                                                                                     <  1:1131673/148‑1 (MQ=255)

                           taataTTATTTCGG‑TCAATATTCA‑AAAATTGTCTAACCTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCat                                                                                                                    <  2:56610/148‑1 (MQ=255)

                                ttattTCGG‑GCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAtttt                                                                                                               >  2:218706/1‑148 (MQ=255)

                                    ttCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAt                                                                                                           >  1:589635/1‑148 (MQ=255)

                                     tCGG‑ACAATATTTA‑AAAATGGTCTAACTGGCGCAATGGCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGTTAAATATGATCGGAATAAAAATGAGTAAGCAAATTTTTGAtt                                                                                                          >  2:681803/1‑148 (MQ=255)

                                      cGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCACTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAttt                                                                                                         >  2:671239/1‑148 (MQ=255)

                                      cGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAttt                                                                                                         >  1:1248154/1‑148 (MQ=255)

                                      cGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATATTTGAttt                                                                                                         >  2:1271494/1‑148 (MQ=255)

                                      cGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCAAATCGTCGAGGCTAAATATGATCGGCATAAAAATGAGTAAGCATAGTTTTGGttt                                                                                                         >  2:697511/1‑148 (MQ=255)

                                       gg‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGGTTTc                                                                                                        >  2:1143225/1‑148 (MQ=255)

                                       gg‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTc                                                                                                        <  1:1154545/148‑1 (MQ=255)

                                           cAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                     >  1:1268664/1‑148 (MQ=255)

                                               atTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGGATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAGAATGAGTAAGCATGTTTTTGATTTCAGGTAtt                                                                                                 >  1:143284/1‑148 (MQ=255)

                                               atTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                                 >  1:645828/1‑148 (MQ=255)

                                               atTTA‑AAAATTGTCTAACTTGCTCAATGCCGCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                                 <  2:457170/148‑1 (MQ=255)

                                               atTTA‑AAAATTGTCTAACTTGCTCAATCCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                                 <  2:1153741/148‑1 (MQ=255)

                                                   a‑aaaaTTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTAc                                                                                             <  1:104011/148‑1 (MQ=255)

                                                         ttGTCTAACTTGCTCCAAGCCTCATGCTTTATAAATACCAAGTTTTACAATTTCAATCACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTAGTtcct                                                                                        >  2:27062/1‑148 (MQ=255)

                                                         ttGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttct                                                                                        >  2:1263052/1‑148 (MQ=255)

                                                         ttGTCTAACTTGCTCAATGCATCTTGCTTAATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATACTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttct                                                                                        >  1:729800/1‑148 (MQ=255)

                                                            tCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGGATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCg                                                                                     >  2:1173765/1‑148 (MQ=255)

                                                            tCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCCGGTATTTTCCTTTCTTCg                                                                                     >  2:791746/1‑148 (MQ=255)

                                                            tCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCg                                                                                     >  2:638802/1‑148 (MQ=255)

                                                            tCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCg                                                                                     >  2:1091195/1‑148 (MQ=255)

                                                             cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                                    >  1:201440/1‑148 (MQ=255)

                                                             cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                                    >  1:1057257/1‑148 (MQ=255)

                                                                aCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCtt                                                                                 <  2:246151/148‑1 (MQ=255)

                                                                aCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCtt                                                                                 <  2:1066180/148‑1 (MQ=255)

                                                                 cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTGAGGTATTTTACTTTCTTCGGATTc                                                                                >  2:1390422/1‑148 (MQ=255)

                                                                 cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCCGGCCTTTTACTTTCTTCGGCTTc                                                                                >  2:217950/1‑148 (MQ=255)

                                                                  ttGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                               >  2:251168/1‑148 (MQ=255)

                                                                  ttGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                               <  1:922126/148‑1 (MQ=255)

                                                                   tGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCaa                                                                              <  2:765747/148‑1 (MQ=255)

                                                                    tcTCAATGCTTCTTGCTTTATTACTACCAAGTTTTACAATTTCAATCACAGGGAATGGTGCGCCCCAGCGTCGATGCCAAATACGACCGGCATAAAAATGAGTCAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAAt                                                                             <  1:347591/147‑1 (MQ=255)

                                                                       cAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaa                                                                          <  1:719648/148‑1 (MQ=255)

                                                                        aaTGCCTCTTGCTTTATTAATACCAAGGTTGACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaa                                                                         >  2:709800/1‑148 (MQ=255)

                                                                         aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCCGGTATTTTACTTTCTTCGGCTTTAATTaaaa                                                                        >  1:889975/1‑148 (MQ=255)

                                                                         aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaaa                                                                        >  1:543479/1‑148 (MQ=255)

                                                                            ccTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                     <  1:1129523/148‑1 (MQ=255)

                                                                            ccTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                     <  1:1150898/148‑1 (MQ=255)

                                                                            ccTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                     <  1:994731/148‑1 (MQ=255)

                                                                            ccTCTTGCTCTATGAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                     <  1:114224/148‑1 (MQ=255)

                                                                             ctctTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGa                                                                    <  2:487778/148‑1 (MQ=255)

                                                                             ctctTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGa                                                                    <  1:568022/148‑1 (MQ=255)

                                                                             ctctTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGa                                                                    <  1:934003/148‑1 (MQ=255)

                                                                                            tACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAACATTGAGGTAGGGGCTTCttt                                                     >  2:674510/1‑148 (MQ=255)

                                                                                               cAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                                  <  2:672536/148‑1 (MQ=255)

                                                                                               cAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                                  >  2:1212020/1‑148 (MQ=255)

                                                                                                aaGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGGGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTCAAATTGAGGGAGAGGATTCTTTCAAg                                                 >  1:588285/1‑148 (MQ=255)

                                                                                                aaGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTCCAGGAACGGTACTTCCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                                 >  2:640545/1‑148 (MQ=255)

                                                                                                  gTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTg                                               >  2:77446/1‑148 (MQ=255)

                                                                                                   ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTgg                                              >  1:576231/1‑148 (MQ=255)

                                                                                                   ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTgg                                              >  1:353227/1‑148 (MQ=255)

                                                                                                   ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTgg                                              >  1:1124788/1‑148 (MQ=255)

                                                                                                       aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACaa                                          >  2:777032/1‑148 (MQ=255)

                                                                                                        cAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAg                                         >  2:695348/1‑148 (MQ=255)

                                                                                                            ttCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTaaa                                     <  1:948758/148‑1 (MQ=255)

                                                                                                                  gACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCa                               >  1:246401/1‑148 (MQ=255)

                                                                                                                   aCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCACGTGGACAAGTAAATTACCAg                              >  2:323595/1‑148 (MQ=255)

                                                                                                                   aCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAg                              >  2:78808/1‑148 (MQ=255)

                                                                                                                   aCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTCCAAGTGGACAAGTAAATTACCAg                              >  2:246057/1‑148 (MQ=255)

                                                                                                                     aGGGAATGGGGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACCAGTAAATTACCAGtt                            <  2:1183205/148‑1 (MQ=255)

                                                                                                                       ggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAGATAAATTACCAGttta                          >  2:954327/1‑147 (MQ=255)

                                                                                                                        gAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGa                         >  1:1326180/1‑148 (MQ=255)

                                                                                                                        gAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGa                         <  1:1406709/148‑1 (MQ=255)

                                                                                                                        gAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATGGAGGAAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGa                         >  1:805650/1‑148 (MQ=255)

                                                                                                                            ggTGCGCCTCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTACGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTc                     <  2:272754/148‑1 (MQ=255)

                                                                                                                            ggTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTc                     <  1:251168/148‑1 (MQ=255)

                                                                                                                            ggTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTc                     <  1:91587/148‑1 (MQ=255)

                                                                                                                             gTGCGCCCCAGCGTCGATGCTAAATATGATCTGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCt                    <  2:132558/148‑1 (MQ=255)

                                                                                                                              tGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCtt                   >  2:104603/1‑148 (MQ=255)

                                                                                                                                    ccAGCGTCGATGCTAAATATGAGCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTa             <  2:894960/148‑1 (MQ=255)

                                                                                                                                     cAGCCTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAc            <  1:1307142/148‑1 (MQ=255)

                                                                                                                                     cAGAGTGGATGCTAAATATGATCGGCATAAAAAGGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTAAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAc            <  2:29014/148‑1 (MQ=255)

                                                                                                                                      aGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAcc           <  1:1212020/148‑1 (MQ=255)

                                                                                                                                      aGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAcc           >  2:439221/1‑148 (MQ=255)

                                                                                                                                           cGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTg      >  1:91160/1‑148 (MQ=255)

                                                                                                                                               gCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACATGTAAATAAGCAGGTTGAATTCTTGGATTACCCATTGaaaa  <  1:681854/148‑1 (MQ=255)

TATCATTATCTTCATCACCGAATGCAATAATATTATTTCGG‑TCAATATTTA‑AAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGAAAA  >  USA300TCH1516_ALE/973069‑973357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`