| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,232,148 | G→T | 100% | G18V (GGC→GTC) | prkC → | Serine/threonine‑protein kinase PrkC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,232,148 | 0 | G | T | 98.0% | 101.8 / ‑5.8 | 38 | G18V (GGC→GTC) | prkC | Serine/threonine‑protein kinase PrkC |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (21/15); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTT‑GAACGAGAAGTACATAACTCATCA > USA300TCH1516_ALE/1232011‑1232284 | gTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAGAGATAGCGTTCCTGTCATATACGATACGAGTGAAGGTGATAAAGTATGATATGTTCAATAATAAATGAAGGATATGAAATTGTAGATAAGCTTGGcgtcgg > 2:335756/1‑141 (MQ=255)gTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGcgtcgg > 2:165311/1‑141 (MQ=255) aTCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTgg > 1:545627/1‑141 (MQ=255) ttAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTACATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCCTGGCGTCGGTGGCATGa > 2:33241/1‑141 (MQ=255) ttAATGCAATTGGCATTAGATAAAAAGTCTAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGAATGa > 2:149494/1‑141 (MQ=255) aTTGGCATTAGATAACCATTCGAAAGATAACGCTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCAGGAGTAACGtt > 1:199591/1‑141 (MQ=255) aTTGGCATGAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGtt > 1:443965/1‑141 (MQ=255) gCATTAGATAACCATTCGAAAGATAACGTTACTTTGATACTCGCGGCTATTGAAGGTGATGAAGTATGATAGGTTAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGGGTGGGTGGCATGGGTACCGTTTAtt > 2:421505/1‑140 (MQ=255) ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTg > 2:438500/1‑141 (MQ=255) aTTCGAAAGAAAACGATACTTTCATACTCGCGGCTATTGAAGGTGAAGTCGGATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatac < 1:226247/141‑1 (MQ=255) ttCGAAAGATAACGTTTCTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacg < 2:332760/141‑1 (MQ=255) tCGAAAAATAACGTTACTTTCATACTCGCGGCTATTGAAGTTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga < 2:334694/141‑1 (MQ=255) aaGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACt > 2:441216/1‑141 (MQ=255) aCGTTACTTTAAGACTCGCAGGTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACAt < 2:112313/141‑1 (MQ=255) gTTACTTTCATTTTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATTATAAATGAACGTTATAAAATTGTAGATAAGCTTGGCGCCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTa < 2:594330/141‑1 (MQ=255) gTTACTTTCATACTCGCGGCTATTGAAGGTGATTAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAAGATTa < 1:473095/141‑1 (MQ=255) gTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAACTCCTGGATTAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATCCTTAACATTa < 1:504316/141‑1 (MQ=255) ttCATACTGGCGGCTATTGAAGGTGATAAAGTATGATCGGTAAAATCATAAATGAGCGATAAAAGAATGTAGATAAGCTTGGCGTCGGGGGCATGAGTAACGTTTAACTTGCTGAAGCTACGCTACTTAAGATTAAAGATg > 2:575660/1‑141 (MQ=255) ttCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTg > 2:116662/1‑141 (MQ=255) cgcgGCTATTGAAGGTGATAAAGTATCATAGGTAAAATAATAAATGAACGATAAAAAATTGTAGATAAGCTTGGGGTCGGAGGAATGAGTACCGTTTATCTTGCTAAAGATACGAGACTTACCATTAATGCTACAATTaat > 2:431990/1‑140 (MQ=255) cgGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGGTGCAATTAAGGc > 1:555270/1‑141 (MQ=255) ttGAAGGTGATAAAGTATGATCGTTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtttt < 1:312219/141‑1 (MQ=255) ttGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGCACGATATACAATTGACACTATACATGGGGGCTTGGTTGTTATTATTGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGGGAtttt > 1:137878/1‑141 (MQ=255) ttGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtttt < 1:126219/141‑1 (MQ=255) ttGAAAGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtttt < 1:555051/141‑1 (MQ=255) aaaaaacaTAAAGACTGATAGGTAAAATAATAAATTAATGAAACAAAATTGCAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTta < 2:32923/134‑1 (MQ=255) gTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCTGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATacc > 2:179677/1‑141 (MQ=255) tAAAGTATGATAAGTAAAAGAATAAATGAAAGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCt < 2:691545/141‑1 (MQ=255) aaaGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTa > 1:430270/1‑141 (MQ=255) aaaGTATGATAGGTAAAATAATAAAGGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTa < 2:82904/141‑1 (MQ=255) gTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTagag < 1:441216/141‑1 (MQ=255) aGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATCAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAaaga < 1:368057/141‑1 (MQ=255) aataatAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGaaaa > 2:160289/1‑140 (MQ=255) aataatAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAAAAGAAAc > 2:277572/1‑141 (MQ=255) aTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAc > 1:673885/1‑141 (MQ=255) taAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCCATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAGGTTTA‑GAACg > 2:172465/1‑141 (MQ=255) aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTT‑TAACGAGAAGTACa > 2:97415/1‑141 (MQ=255) aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTT‑GAACGAGAAGTACa < 1:152051/141‑1 (MQ=255) aGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTT‑GAACGAGAAGTACATAACt > 1:99365/1‑141 (MQ=255) ttGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTT‑GAACGAGAAGTACATAACtcat > 1:666407/1‑141 (MQ=255) ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTAGAACG‑GAAGTACATAACtcataa > 2:105890/1‑139 (MQ=255) | GTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTT‑GAACGAGAAGTACATAACTCATCA > USA300TCH1516_ALE/1232011‑1232284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |