| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,775,841 | T→C | 85.2% | H228R (CAT→CGT) | clpX ← | ATP‑dependent Clp protease ATP‑binding subunit ClpX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,775,841 | 0 | T | C | 85.2% | 177.8 / 16.0 | 76 | H228R (CAT→CGT) | clpX | ATP‑dependent Clp protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base T (5/7); new base C (33/31); total (38/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CTTCATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTT > USA300TCH1516_ALE/1775710‑1775976 | cTTCATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTc > 1:485732/1‑141 (MQ=255)cTTCATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTc > 1:594019/1‑141 (MQ=255)cTTCATTGCTTCAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTc < 1:531012/141‑1 (MQ=255) cATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTACTCACTTCTTCCATACGAACAAAGGCACCACCAAGAATAAATAAAAGATTTGTGGTATCAATTTGAATCATCTAATGGTTTTGATGTTTgtgtaccc > 2:206628/1‑137 (MQ=255) cATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCCCCAAGAATAAATCAAATATTTGTTGTATCAATTTGAATCAGTTTTTGGTTTGAATGTTTGCGttgaa > 2:660812/1‑137 (MQ=255) cATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGCATCATTTCTTGGTTTGGATGTTTGCGTCTAc > 2:231103/1‑141 (MQ=255) ttGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTCGCGTCCACCt < 1:502646/141‑1 (MQ=255) ttGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCt > 2:548297/1‑141 (MQ=255) ttGCTTGAGAAACCAATAACTTTATCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCt > 2:496688/1‑141 (MQ=255) ttGCTTGAGAAACCAATAACTTGTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCt > 2:507270/1‑141 (MQ=255) tGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCAACtt > 1:596162/1‑141 (MQ=255) cTTGAGAAACCAATGACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGCTATTTCGTGTATCAAGTTGCAGCATTTCGTGGTTTGGACGTTTGCGTCCACCTtgt < 2:665920/141‑1 (MQ=255) aCCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAGTCAATTCTTAGTTTGGACGTTTGGGTCCACATATTGGCAGaaa > 2:201524/1‑140 (MQ=255) aCCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAac > 1:253380/1‑141 (MQ=255) aCCAATAACTTTTTCACCAAGACGGCGCTTAATCACCTTTTCAATAACATCAAAGGAACCACAAAGAATAAATAAGATATTTGATGTATACACTTGCATCATGTCTTGCTTTAAACGTTAGCGTCCACCTTGTGGCGGacc > 2:403786/1‑139 (MQ=255) cAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAacac < 2:85267/141‑1 (MQ=255) aTAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTAGTAACAATTTGAATCATTTCTTGGTTTGGACGTTAGCGTCCACCTTGTGGCGGAACACtg > 1:92872/1‑140 (MQ=255) cTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAg < 1:554917/141‑1 (MQ=255) tttttCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGt < 2:439428/141‑1 (MQ=255) tttcCACCAAGCCATCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTc < 2:34030/141‑1 (MQ=255) ttCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGt > 1:40011/1‑141 (MQ=255) ttCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGt > 1:345089/1‑141 (MQ=255) ttCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTCGAATCGCTTCTTGGATTGGACGTTTGCGTCCACCTTGTGGCGGAACTGTTGCAGTCGt > 1:648552/1‑141 (MQ=255) tCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTCGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTa > 2:576372/1‑141 (MQ=255) tCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTCGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTAGCGGGACACTTGCAGTCTTa > 2:266626/1‑141 (MQ=255) ccAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCt < 2:335919/141‑1 (MQ=255) cAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCtt < 2:462810/141‑1 (MQ=255) gcgcTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGAt < 2:631326/141‑1 (MQ=255) cgcTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCAATTCTTTATTTGAACGTTTGTGTCCACCTTGTGGCGGAACACTTCCAGTTGTACCTTCTatgttt > 2:656283/1‑136 (MQ=255) gcTTAATCACTTCTTCAATACCATCCAAGGCACGCCCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAACAttt < 1:473204/141‑1 (MQ=255) aaTCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAg > 1:594040/1‑141 (MQ=255) aaTCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAg > 1:594188/1‑141 (MQ=255) ttcAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCa > 2:490419/1‑134 (MQ=255) ttcAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCa < 1:490419/134‑1 (MQ=255) tcAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCttgt > 2:126714/1‑141 (MQ=255) tcAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCttgt > 1:602584/1‑141 (MQ=255) aTACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGa < 1:660554/141‑1 (MQ=255) aTACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGa < 1:95657/141‑1 (MQ=255) tACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGaa < 2:573920/141‑1 (MQ=255) aCCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTCCCACTTTGAAACATCTCTTGGTTTGGACGTTTGCGTCCACCTGCTGGCGGAATACTTGCAGTCGTACCATCTAAGACTTTAAGCAATACTTGTTGacc > 2:96118/1‑139 (MQ=255) cAAAGGCACCACCAAGAATAAATAAGATTTTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCAACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTACGCAATGCTTGTTGAACACCtt < 1:482353/141‑1 (MQ=255) cAAAGGCACCACCAAAAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCTGAACACTTGCAGTCGTACCTTCTAATATTTTAATCAATGCTTGTTGAACACCtt < 1:450002/141‑1 (MQ=255) aagggCACCACCGAGGATAAATAAGGGATTTGTTGTATCAATTTGAATCCTTTCTGGGTTTGGACGTGTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTTTGTAATGCTTGTTGAATACCTTc < 2:301272/138‑1 (MQ=255) ggCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCAcc > 2:54877/1‑141 (MQ=255) ggCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCAcc < 1:285199/141‑1 (MQ=255) ggCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCAcc < 2:330018/141‑1 (MQ=255) caccacCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTg > 1:290489/1‑141 (MQ=255) caccacCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATCCTTGCGTAACACCTTCGCCTg > 1:262212/1‑141 (MQ=255) ccaccaAGAATAAATAAGGTATTTCTTGTATCAATTTGAATCATTTCTTTGTTTGGACGTTTGCGGCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGaa < 1:660850/141‑1 (MQ=255) caccaAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACCCTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGaaa < 1:507270/141‑1 (MQ=255) caccaAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCGAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCCGaaa < 1:496688/141‑1 (MQ=255) caccaAGAATAAATAAGAGATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGaaa < 2:311649/141‑1 (MQ=255) aaGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTc < 1:54877/141‑1 (MQ=255) aaGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTc < 1:586516/141‑1 (MQ=255) taaGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGATCACCTTCACCTGAAACGTCACGTGTtat < 1:441231/141‑1 (MQ=255) gATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTCGACGTTTGGTTCCAGCGTGTGGCGGAACACTTGCAGTCGTACCTTCTCAGATTTTAAGCCATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGa < 2:72440/141‑1 (MQ=255) tttGTTGTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGAtgtg < 1:243467/141‑1 (MQ=255) ttgttgTATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATgtgt < 1:548297/141‑1 (MQ=255) ttgtATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGtttt < 1:161847/141‑1 (MQ=255) tgtATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCTCCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTc > 2:348887/1‑141 (MQ=255) tgtATCAATTTGAATCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAGCGTCACGTGTTATAGATGTGTTTTc > 1:167956/1‑141 (MQ=255) aTTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCCCCTGAAACGTCACGGGTTATAGAAGTGTTTTCAGATTTa > 1:488759/1‑141 (MQ=255) tttGAATCATTTCTTGGTTTGGCCGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGCAACGTCAAGTGTTATAGATGTGTTTTCAGATTTAc > 2:291018/1‑141 (MQ=255) aaTCGTTTCTTGATTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAACCGTCACGTGTTATAGGTGTGTCTTCAGATTTACGTGc > 1:490460/1‑141 (MQ=255) aaTCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGc > 2:41404/1‑141 (MQ=255) aaTCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGc < 1:532443/141‑1 (MQ=255) aaTCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCAACTGAAACGTCACGTGTTATAGATGTGTTTTCAGCTGTACGTGc > 2:554077/1‑141 (MQ=255) tCATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCaa > 1:325949/1‑141 (MQ=255) cATTTCTTGGTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTGATAGATGTGTTTTCAGATTTACGTGCatt > 1:40555/1‑139 (MQ=255) cATTTCTTGGTTTGGACGTTGGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAAt < 1:295177/141‑1 (MQ=255) tGGTTTGGACGGTTGCGTCCACCTTGTGGCGGACCACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTTAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCa < 1:35521/141‑1 (MQ=255) tGGTGTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCa < 1:574784/141‑1 (MQ=255) gTTTGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAAt > 2:567014/1‑141 (MQ=255) gttGGACGTTTGCGTCCACCTTGTGGCGGAACCCTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAAtt < 1:150923/140‑1 (MQ=255) ttGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAAttt < 1:373844/141‑1 (MQ=255) ttGGACGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAAttt < 2:412261/141‑1 (MQ=255) | CTTCATTGCTTGAGAAACCAATAACTTTTTCACCAAGACGGCGCTTAATCACTTCTTCAATACCATCAAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTT > USA300TCH1516_ALE/1775710‑1775976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |