Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 307,880 T→C 7.6% L43P (CTG→CCG)  lytR_1 → Sensory transduction protein LytR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE307,8800TC 7.6% 161.7 / 4.4 65L43P (CTG→CCG) lytR_1Sensory transduction protein LytR
Reads supporting (aligned to +/- strand):  ref base T (35/24);  new base C (3/2);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

GAGGAATAAATATGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGACCAATACGCAGTACA  >  USA300TCH1516_ALE/307742‑308018
                                                                                                                                          |                                                                                                                                          
gaggaATAAATATGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGa                                                                                                                                          <  1:532621/141‑1 (MQ=255)
    aataaggaTGAAAGCATTAATCATTGTTGATGAGCCATTAGCACGTAATGAAGTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGATATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGatcaa                                                                                                                                      <  1:173809/141‑1 (MQ=255)
    aataaataTGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGatcaa                                                                                                                                      <  1:256800/141‑1 (MQ=255)
    aataaataTGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGatcaa                                                                                                                                      <  1:353944/141‑1 (MQ=255)
      taaataTGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAAATGGTGGATTTGAAGAACTTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGatcaatc                                                                                                                                    >  2:469038/1‑141 (MQ=255)
      aaaataTGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATAAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGatcaatc                                                                                                                                    <  1:652116/140‑1 (MQ=255)
       aaataTGAAAGCATTAATCATAGATGGTGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGatcaatca                                                                                                                                   <  2:282615/141‑1 (MQ=255)
            tGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATg                                                                                                                              >  2:368027/1‑141 (MQ=255)
                 gCATTAATCAGAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTTGAAGCACTACTGATCAATCAATATGACAtt                                                                                                                         <  2:572347/141‑1 (MQ=255)
                             gatgatGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGAt                                                                                                             >  2:11233/1‑141 (MQ=255)
                              atgatgAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATCATATTTTTAGATg                                                                                                            >  2:400971/1‑141 (MQ=255)
                              atgatgAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACCGATCAATCAATATGACATTATATTTTTAGATg                                                                                                            >  2:107487/1‑141 (MQ=255)
                              atgatgAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACCGATAAATCAATATGACATTATATTTTTAGATg                                                                                                            >  2:363448/1‑141 (MQ=255)
                              atgatgAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAAGGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGACCAATCAATATGACATTATATTTTTAGATg                                                                                                            >  1:230784/1‑141 (MQ=255)
                               tgatgaGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGt                                                                                                           >  2:213386/1‑141 (MQ=255)
                               tgatgaGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATAGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGt                                                                                                           >  1:487789/1‑141 (MQ=255)
                               ggatgaGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGGTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGt                                                                                                           <  1:416844/140‑1 (MQ=255)
                                  tgaGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCaa                                                                                                        >  1:80317/1‑141 (MQ=255)
                                   gaGTCATTAGCAAGTAATGAATTAACAGAGTTATTAAATGAAATTGGTGAGTTATAAGAAATTAATGAGGCAGAAAATGTAAAAGATACTTTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAAt                                                                                                       <  2:621096/141‑1 (MQ=255)
                                   gaGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAAt                                                                                                       >  2:506587/1‑141 (MQ=255)
                                   gaGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGGGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGCCATTATCTTTTTAGATGTCAAt                                                                                                       >  2:131081/1‑141 (MQ=255)
                                    aGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAAtt                                                                                                      >  2:179792/1‑141 (MQ=255)
                                     gCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAAttt                                                                                                     >  2:30665/1‑141 (MQ=255)
                                      ccATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAAtttc                                                                                                    >  1:155161/1‑140 (MQ=255)
                                      ccATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTa                                                                                                    >  2:84218/1‑141 (MQ=255)
                                      ccATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACAATGTAAGCACTACCGATCACCCAAGATGTCATTATATTTTTAGATGTCAATTTa                                                                                                    >  1:609640/1‑141 (MQ=255)
                                      ccATTAGCACGTAATGAATTAACATATTGATTAAACGAACTTGGTGGTTTTGACGAGATTAATGAGGCAGAAAATGTAAAGGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATAATTTTAGATGTCAAGTTa                                                                                                    >  2:275619/1‑141 (MQ=255)
                                           aGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGa                                                                                               <  1:69693/141‑1 (MQ=255)
                                                     gAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATggg                                                                                     >  1:83467/1‑141 (MQ=255)
                                                     gAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATggg                                                                                     <  1:368027/141‑1 (MQ=255)
                                                       aTTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAAAGGGAt                                                                                   >  2:126960/1‑141 (MQ=255)
                                                            cATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAAt                                                                              >  2:385672/1‑141 (MQ=255)
                                                                 ttattaAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGCTGAAAATGGGATCGAATTAGGa                                                                         >  2:212442/1‑141 (MQ=255)
                                                                 ttattaAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGGAAATGGGATCGAATTAGGa                                                                         >  1:28760/1‑141 (MQ=255)
                                                                    ttaAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCt                                                                      >  2:678283/1‑141 (MQ=255)
                                                                    ttaAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCt                                                                      >  2:333123/1‑141 (MQ=255)
                                                                    ttaAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATAGAATTAGGAGCt                                                                      >  1:218345/1‑141 (MQ=255)
                                                                       aaTGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAggtgatagg                                                                   >  2:68072/1‑134 (MQ=255)
                                                                            aaTTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATGAGAAGCTCTACTGATCAATCAATATGACATTATATTTTTATATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGACCTAGGCTTCa                                                              >  2:115758/1‑141 (MQ=255)
                                                                                ggtggtTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAg                                                          >  1:429313/1‑141 (MQ=255)
                                                                                  tggtTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAACATGGGATCAAATTAGGAGCTAAGATTCAAAAgga                                                        >  2:62169/1‑139 (MQ=255)
                                                                                   ggtTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCAAAGATTCAAAAGAta                                                       >  2:390705/1‑140 (MQ=255)
                                                                                       ttGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAag                                                   <  2:658687/141‑1 (MQ=255)
                                                                                         gaagaaATTAATGAGGCAGAAAATGTAAAAGAGACGTTGGAAGCACTACTGATCAATAAATATGACAATATATTTTTAGATGTCAATTTAATGGATGAAAATGGGAACGAATTAGGAGCTAAGATTCAAAAGATGAAagag                                                 >  2:257023/1‑141 (MQ=255)
                                                                                              aagtAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGATCTAAGATTCAAAAGATGAAAGAGCCAcc                                            <  1:336855/138‑1 (MQ=255)
                                                                                              aaTTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCAcc                                            <  2:11713/141‑1 (MQ=255)
                                                                                               aTTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTGTATTATTAGATGTCAATATAATGGATGAAAATGGGATCGAATTAGGAGCAAAGATTCAAAAGATGAAAGAGCAACCt                                           >  2:419034/1‑141 (MQ=255)
                                                                                                  actctggCAGCAAATGCAAAAGAAACATGGGAAGCACTACTGATCAATCAACATGACATTATATTTTTAGATGTCAATTCAATGGACTAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCg                                        <  1:1493/136‑1 (MQ=255)
                                                                                                  aaTGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCg                                        <  2:604354/141‑1 (MQ=255)
                                                                                                  aaTGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTAACTCTCCCAGAGGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCg                                        >  1:332018/1‑141 (MQ=255)
                                                                                                         cAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTAttt                                 <  2:245728/141‑1 (MQ=255)
                                                                                                         aaGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGACTTCGGAGTTGAGATTCAAAAGATGAAAGAGCCACCTGCGATTAttt                                 <  2:286511/140‑1 (MQ=255)
                                                                                                            aaaaTGTAAAATAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTg                              <  1:309035/141‑1 (MQ=255)
                                                                                                            aaaaTGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTg                              <  1:414944/141‑1 (MQ=255)
                                                                                                            aaaaTGTAAAAGAAACATTGGAAGCACTACCGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTg                              <  1:107487/141‑1 (MQ=255)
                                                                                                            aaaaTGTAAAAGAAACATTGGAAGCACTACCGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTg                              <  1:363448/141‑1 (MQ=255)
                                                                                                                tGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAAc                          <  1:11233/141‑1 (MQ=255)
                                                                                                                    aaaGACACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGca                      <  2:367946/141‑1 (MQ=255)
                                                                                                                    aaaGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGca                      >  2:308495/1‑141 (MQ=255)
                                                                                                                         aaCATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGa                 <  2:83467/141‑1 (MQ=255)
                                                                                                                          aCATTTGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGAc                <  2:593463/141‑1 (MQ=255)
                                                                                                                          aCATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAACTGGGATCGAATTAGGAGCTAAGATTCAAAAAATGAAAGAGCCCCCTGCGATTCTTTTTGCAACTGCACATGAc                >  2:641575/1‑141 (MQ=255)
                                                                                                                          aCATTGGAAGCACTACGGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGAc                >  1:348235/1‑141 (MQ=255)
                                                                                                                                gAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGACCAATAc          >  2:688674/1‑141 (MQ=255)
                                                                                                                                   gCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCAACCTGCGATTATTTTTGCAAATGCACAAAACCAATACGCa       >  2:449127/1‑141 (MQ=255)
                                                                                                                                        actGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGACCAATACGCAGTACa  >  2:33082/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GAGGAATAAATATGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTAAATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAAGCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAATGGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCAACTGCACATGACCAATACGCAGTACA  >  USA300TCH1516_ALE/307742‑308018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑