Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 348,452 A→G 5.7% E130G (GAA→GGA)  yezG_5 → putative antitoxin YezG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE348,4520AG 5.7% 199.3 / 2.1 70E130G (GAA→GGA) yezG_5putative antitoxin YezG
Reads supporting (aligned to +/- strand):  ref base A (30/36);  new base G (2/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GGAACTAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTT  >  USA300TCH1516_ALE/348312‑348588
                                                                                                                                            |                                                                                                                                        
ggAACTAAGAGAAGTATTTATGGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGa                                                                                                                                          >  1:361067/1‑141 (MQ=255)
ggAACTAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAAATTGATTTTACAAAAGAAGATAAATAAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGa                                                                                                                                          <  1:526969/141‑1 (MQ=255)
    cTAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGACCAATTAGGCCGTGGAAat                                                                                                                                      >  2:564058/1‑141 (MQ=255)
    cTAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATCTGATTATATTGATTGGATAAATACAGAGTTTGACCAATTAGGCCGTGGAAat                                                                                                                                      >  2:564125/1‑141 (MQ=255)
    atAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAat                                                                                                                                      <  2:587655/140‑1 (MQ=255)
       agagAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATGTTACAAAAGAAGGTAAATTAAAAGTTTCATGTGATTATGTTGATTGGATAAATAGAGAGTTTGGGCAAGTGGGCGGTGAAAattgg                                                                                                                                   >  1:315815/1‑139 (MQ=17)
              tATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGt                                                                                                                            >  2:139865/1‑141 (MQ=255)
               aTTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATGTGTa                                                                                                                           >  1:656512/1‑141 (MQ=255)
                tttATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTAc                                                                                                                          <  2:213264/141‑1 (MQ=255)
                 ttATAGGAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACa                                                                                                                         <  2:682082/141‑1 (MQ=255)
                 ttATAAAAGTACGTTTTGAACCACGGACCTCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACa                                                                                                                         <  1:256778/141‑1 (MQ=17)
                   ataGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGTGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACaaa                                                                                                                       <  1:496086/141‑1 (MQ=255)
                     agaagaagaTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCTATTAGGCCGTTAAAATTATTAAAAATAaaaaaa                                                                                                                     <  2:494612/141‑1 (MQ=17)
                     agaagaAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACaaaaa                                                                                                                     <  2:494697/141‑1 (MQ=255)
                      gaagaaGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACaaaaaa                                                                                                                    >  2:124769/1‑141 (MQ=255)
                       aagtagGTCTTGAACCACGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGCTTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAAt                                                                                                                   <  2:465160/141‑1 (MQ=255)
                       aagaagGGCGTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGACAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAAt                                                                                                                   <  2:349239/141‑1 (MQ=255)
                         gaagGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAAttt                                                                                                                 >  1:255674/1‑141 (MQ=255)
                          aagGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTACTATATGTACAAAAAATTTg                                                                                                                <  1:417535/141‑1 (MQ=255)
                          aagGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGACCAATTAGGCCGTGGAAATTATTATATGTACAAAAAATTTg                                                                                                                <  1:564058/141‑1 (MQ=255)
                          aagGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATCTGATTATATTGATTGGATAAATACAGAGTTTGACCAATTAGGCCGTGGAAATTATTATATGTACAAAAAATTTg                                                                                                                <  1:564125/141‑1 (MQ=255)
                           agGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTgg                                                                                                               <  2:113680/141‑1 (MQ=255)
                            ggTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGgt                                                                                                              >  1:196000/1‑141 (MQ=255)
                            ggTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATGGGATAAATACAGAGTTTGATCAATCAGGCCGTGAAAGTTATTATATGTACAAAAAATTTggg                                                                                                              >  1:224297/1‑140 (MQ=255)
                               cTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGtt                                                                                                           >  2:27622/1‑141 (MQ=255)
                                  gAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTa                                                                                                        >  1:546378/1‑141 (MQ=255)
                                  gAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTa                                                                                                        >  1:375482/1‑141 (MQ=255)
                                  gAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTa                                                                                                        >  1:519375/1‑141 (MQ=255)
                                  gAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTa                                                                                                        >  2:368843/1‑141 (MQ=255)
                                  gAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTCAAAATTATTATATGTACAAAAAATTTGATGTTTTa                                                                                                        >  2:126859/1‑141 (MQ=255)
                                    aCCATGGACATCATGCGAATTTGACTTTACGAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCATTTAGGCCTTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTAcc                                                                                                      <  2:435628/141‑1 (MQ=255)
                                     ccATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCa                                                                                                     >  1:522316/1‑141 (MQ=255)
                                     caaTGGACATCATGCGAATTTGATTTTACAACAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTATTTTACCa                                                                                                     >  2:107002/3‑141 (MQ=255)
                                        tGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGaa                                                                                                  <  1:70281/141‑1 (MQ=255)
                                         ggACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCACTTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGaaa                                                                                                 <  1:367140/141‑1 (MQ=255)
                                          gACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAAGTATTATATGTACTAAAGATTTGGTGTTTTACCAGAAAc                                                                                                >  1:608871/1‑141 (MQ=255)
                                              tcatGCGAATTTGATTTTACGAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGaa                                                                                            <  2:357261/141‑1 (MQ=255)
                                               catGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAat                                                                                           >  1:648600/1‑141 (MQ=255)
                                                   ggAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGa                                                                                       <  2:192316/140‑1 (MQ=255)
                                                   cGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGa                                                                                       <  1:150911/141‑1 (MQ=255)
                                                   cGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGa                                                                                       <  1:42785/141‑1 (MQ=255)
                                                      aTTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAAt                                                                                    <  2:609110/141‑1 (MQ=255)
                                                           aTTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAAGggagg                                                                               >  2:330248/1‑141 (MQ=255)
                                                                aCAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGtt                                                                          >  1:184566/1‑141 (MQ=255)
                                                                 cAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTa                                                                         <  1:139865/141‑1 (MQ=255)
                                                                 cAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATAGGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTa                                                                         >  2:487996/1‑141 (MQ=255)
                                                                 aaaaaGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTa                                                                         <  1:375910/140‑1 (MQ=255)
                                                                  aaaaGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTaa                                                                        <  2:186160/141‑1 (MQ=255)
                                                                    aagaagATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAAGGGAGGAGGTTAAAg                                                                      <  2:275217/141‑1 (MQ=255)
                                                                     agaagaTAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGCTAAAGa                                                                     >  1:429773/1‑141 (MQ=255)
                                                                         gaTAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATAAGAAAAGGAGGCGGATAAAGaaacc                                                                 >  2:193377/1‑139 (MQ=255)
                                                                           tAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATTCAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGa                                                               >  2:470347/1‑141 (MQ=255)
                                                                                    aGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGGGGAGGTTAAAGAAATCGAGCAatatat                                                      >  1:120114/1‑141 (MQ=255)
                                                                                            ttGATTATATTGATTGGAAAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGCCCAAGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGc                                              <  1:306245/141‑1 (MQ=17)
                                                                                                  atatTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTAATATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCaagaag                                        <  2:435117/141‑1 (MQ=255)
                                                                                                    attgattgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAgaagaa                                      >  1:432980/1‑141 (MQ=255)
                                                                                                         ttgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGAc                                 <  2:314364/141‑1 (MQ=255)
                                                                                                           gtaTAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACaa                               <  1:567190/139‑1 (MQ=255)
                                                                                                           ggATAAATACAGAGGTTGATCCATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACaa                               <  1:21612/141‑1 (MQ=255)
                                                                                                                aaTACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGGTAAAGCTCTCGCACAATATATTAAAGAGCAAGAAGAATAGACAATCTTa                          <  1:335344/141‑1 (MQ=17)
                                                                                                                 aTACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGGTAAAGAAATCGAGCAATATATGAAAGAGAAAGAAGAATAGACAATATTaa                         >  1:94977/1‑141 (MQ=255)
                                                                                                                  tACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAAt                        >  1:288800/1‑141 (MQ=255)
                                                                                                                    cAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGt                      <  1:110095/141‑1 (MQ=255)
                                                                                                                    aagagTTTGATCAATTAGGCCGTGAAAAATAAAAAAAAAAAAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGt                      <  2:419865/140‑1 (MQ=18)
                                                                                                                      gagTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGGGGTTAAAGAAATCGAGCAAGATATTAAAGAGCAAAAAGAATAGAAAATCTTAATGGaa                    >  1:229927/1‑141 (MQ=18)
                                                                                                                      gagTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATATTAATGTaa                    >  2:203890/1‑141 (MQ=255)
                                                                                                                      gagTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGAAAGAAGAATAGACACTCTTAATGTaa                    >  2:631460/1‑141 (MQ=255)
                                                                                                                                   tAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAGCGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATaa       <  2:224297/141‑1 (MQ=255)
                                                                                                                                   tAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATaa       <  2:196000/141‑1 (MQ=255)
                                                                                                                                    aGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATaaa      <  2:70667/141‑1 (MQ=255)
                                                                                                                                        cgggAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCtt  <  2:240335/138‑1 (MQ=255)
                                                                                                                                        cGTGAACATTACTATAGGTACAAAAAATTTGCTGTTTTAGCAGAAACAGAATATGAAATGGAGGAAGTTAAAGAAATCGAGCAATATATCAAAGAGGAAGAAGAATAGACAATCTGAATGTAAGATTAAACAATAAAGCtt  <  2:182859/141‑1 (MQ=18)
                                                                                                                                            |                                                                                                                                        
GGAACTAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTT  >  USA300TCH1516_ALE/348312‑348588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑