| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,232,148 | G→T | G18V (GGC→GTC) | prkC → | Serine/threonine‑protein kinase PrkC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,232,148 | 0 | G | T | 98.6% | 211.9 / NA | 71 | G18V (GGC→GTC) | prkC | Serine/threonine‑protein kinase PrkC |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (42/28); total (42/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCA > USA300TCH1516_ALE/1232010‑1232284 | ggTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGgcgtcg > 1:260035/1‑141 (MQ=255)ggTGATCAATTAATGCAATTGGCATTAGATAACCATTAGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTGTGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCGTGgcgtcg > 1:152677/1‑141 (MQ=255) cAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCg < 2:708240/141‑1 (MQ=255) cAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCg < 2:21350/141‑1 (MQ=255) aaTTGGCATTAGATAACCATTCGAAAGATAGCGTTACTGTCACACTCGCGTTTATTGACTGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGACAAGCTTGGCGTCGGTGGCATGAGTACCGt < 2:758219/141‑1 (MQ=255) aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCTGCGGTGGCATGAGTACCGt < 1:528791/141‑1 (MQ=255) aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGt < 2:854836/141‑1 (MQ=255) aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGt < 2:670864/141‑1 (MQ=255) aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGt < 1:93690/141‑1 (MQ=255) aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGCCTATTGAAGGTGCTAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGAGGCATGAGTACCGt < 2:679542/141‑1 (MQ=255) aaTGGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATCAGCTTTGCGTCGGTGGCATGAGTACCGt < 2:960992/141‑1 (MQ=255) ttGGCATTAGATAACCATTCGAAAGAAAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGttt < 1:998621/141‑1 (MQ=255) tGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTCTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAAATAAGATTGGCGTCGGTGGCATGAGTACCGTTTa > 1:9331/1‑141 (MQ=255) tGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGAATAAGAACCGTTTa > 1:522187/1‑141 (MQ=255) tGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTAGGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGACATGAGTACCGTTTa > 1:9432/1‑141 (MQ=255) gCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATc > 1:881286/1‑141 (MQ=255) cATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCt < 1:872244/141‑1 (MQ=255) ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTGTCTTg > 1:14325/1‑141 (MQ=255) ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTg > 1:941017/1‑141 (MQ=255) ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGGGGCAGGAGTACCGTTTATCTTg > 1:437815/1‑141 (MQ=255) aTAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGGTAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGa > 1:968573/1‑141 (MQ=255) aTAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGa > 1:943958/1‑141 (MQ=255) tCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga > 1:220055/1‑141 (MQ=255) aTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTaa > 1:939625/1‑141 (MQ=255) cGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACAtt > 1:913998/1‑141 (MQ=255) ttACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATCCGATACTTAACATTaa > 2:427869/1‑141 (MQ=255) aTACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGaaa > 1:330224/1‑141 (MQ=255) aTACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTACTGAAGATACGATACTTAACATTAAAGTTGaaa > 2:815328/1‑141 (MQ=255) cTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTa < 2:822916/141‑1 (MQ=255) cTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTa > 1:355042/1‑141 (MQ=255) cgcgGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAg > 1:206396/1‑141 (MQ=255) aTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAGGGCGAttt < 1:812024/141‑1 (MQ=255) aTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAttt < 1:812061/141‑1 (MQ=255) aTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCGTGGCGTGGGAGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtgt > 2:852973/1‑139 (MQ=255) tGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGCAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAttttt < 2:141882/141‑1 (MQ=255) tGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAttttt < 2:409179/141‑1 (MQ=255) aGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTtata < 2:984753/141‑1 (MQ=255) gTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaca > 1:720774/1‑140 (MQ=255) gATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccac > 1:292765/1‑141 (MQ=255) gATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccac > 1:216715/1‑141 (MQ=255) aTAAAGTATGGTAGGTAAAATAATATATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccacc < 2:921663/141‑1 (MQ=255) aTAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccacc < 1:887831/141‑1 (MQ=255) aTAAAGTATGATAGGTAAAATAATAAATAAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTAGCAATTAAGGCGCTTTTTATaccacc > 1:630828/1‑141 (MQ=255) tAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCt < 1:791537/141‑1 (MQ=255) tAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCAACt > 2:150908/1‑141 (MQ=255) aaaGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTCACATTAAAGCTGCAATTAAGGCGATTTTTATACCACCTa > 2:534355/1‑141 (MQ=255) aaaGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATGAAGGCGATTTTTATACAAGCTa > 2:930141/1‑141 (MQ=255) aGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTaga > 1:253924/1‑141 (MQ=255) gTATGATAGGTAAAATAATAAATGAACGAGATAGAATTGTAGATAAGCTTGGCATCGGTGAAATGAGTACCGTGTATCTTGGTGAAGAGACGATACTTAACATTAAAGTGTCAAATAAGGCGAATGTAATACAACCttgga > 2:537628/1‑137 (MQ=255) tGATAGGTAAAATAATAAATGAACGATATAAAATGGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGACGATTTTTATACCACCTAGAGaaa > 1:3794/1‑141 (MQ=255) taTAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGaaaa < 2:910485/140‑1 (MQ=255) gTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATATTTATCCCACCTAGGAAAAaaaaag > 2:241520/1‑141 (MQ=255) taataaATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACAt < 1:804619/141‑1 (MQ=255) aataaATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACAtt < 2:990867/141‑1 (MQ=255) aaaTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACGTAGAGAAAAAGAAGAAACATTaaa < 1:724973/141‑1 (MQ=255) aaaTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTaaa < 1:840921/141‑1 (MQ=255) aTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAc > 1:586576/1‑141 (MQ=255) aTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGCTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAc > 1:294873/1‑141 (MQ=255) cGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGCGGAGAAGAAGAAACATTAAAACGtttt > 2:870201/1‑141 (MQ=255) atatAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGa > 1:527869/1‑141 (MQ=255) atatAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGa > 2:331746/1‑141 (MQ=255) atatAAAATTGTAGATAAGCTTGGCGTCGGTCGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAGCGttttta > 2:767345/1‑139 (MQ=255) aaaaTTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACga > 1:873608/1‑141 (MQ=255) aaaaTTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATAAGATACTTAACATTAAAGTTGCAATTAAGAGGATTTTTATACAACATGGAGAAAAAGAAGACGAATGAAAATGTTTGGAACgg > 2:614053/1‑140 (MQ=255) tGTAGATAAGCGTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGt < 2:741802/141‑1 (MQ=255) aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAGGTTTTGAAGCAGGAGTaac > 2:708888/1‑139 (MQ=255) aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACa > 1:90812/1‑141 (MQ=255) gCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtc < 1:686783/141‑1 (MQ=255) gCTCGGCGTCGGTGGCATGAGCACCGTTTATCATGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtc < 2:474661/141‑1 (MQ=255) cTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGATGTGAACGAGAAGTACATAAcaca > 2:156466/1‑141 (MQ=255) cTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGAGACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtca > 2:634909/1‑141 (MQ=255) ttGGCGTCGGTAGCATGAGTACCGTTTAAATAGCAGAAGATCCGATACTAAACATAAAAGTTGCAATTAAGGCGAGTTTAATACCACCTATAGAAAAAGAAGAAACATTAAAACGATTAGAACGAGAAGTACATaaaccat > 2:196446/1‑136 (MQ=255) tGGCGTCGGTGGTATTAGTATCGTTTATCTATCTGAAGATACGATACTTAACATTAAAGTTTTACTTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAATACGTTTTGAACGAGAAGTACATAACtcatc < 1:354140/141‑1 (MQ=255) ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcatca > 2:727853/1‑141 (MQ=255) ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcatca < 2:355042/141‑1 (MQ=255) ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcatca > 1:442247/1‑141 (MQ=255) | GGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCA > USA300TCH1516_ALE/1232010‑1232284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |