Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,416,458	A→T	I216N (ATT→AAT)	oppD_4 ←	Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,416,458	0	A	T	100.0%	277.8 / NA	79	I216N (ATT→AAT)	oppD_4	Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (45/34);  total (45/34)

AACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  USA300TCH1516_ALE/1416325‑1416594                                                                                                                                      |                                                                                                                                          aaatCAATCATAGTAATCCCTCTTTAATTTTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                    <  1:373717/138‑1 (MQ=255) aaCTCAATCATAGTAATTCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                    <  1:531613/141‑1 (MQ=255) aaCTCAATCATAGTAATCCCTTTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                    <  2:508650/141‑1 (MQ=255) aaCTCAATCATAGTAATCCCTCTTTGGCGAAGATCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTGTTAAAACATAATTGTACTg                                                                                                                                    >  1:987334/1‑141 (MQ=255)  aCTCAATCTTAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                   <  2:855035/141‑1 (MQ=255)  aCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATGCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                   <  1:135390/141‑1 (MQ=255)  aCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCCTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                   <  2:497680/141‑1 (MQ=255)  aCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATGACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                   <  1:140905/141‑1 (MQ=255)     cAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACaa                                                                                                                                <  1:864827/141‑1 (MQ=255)        tCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAatat                                                                                                                             >  2:551379/1‑141 (MQ=255)         cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGAACTTCTTTTAAAACTTAATTGTACTGACAATAtt                                                                                                                            >  2:2119/1‑141 (MQ=255)                aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATAc                                                                                                                     <  2:851672/141‑1 (MQ=255)                    ctctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATGGTACTGACAATATTTCAATACATGa                                                                                                                 >  1:116589/1‑141 (MQ=255)                       tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                              <  2:35308/141‑1 (MQ=255)                       tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                              <  2:45790/141‑1 (MQ=255)                       tttAATGGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                              <  2:590417/141‑1 (MQ=255)                           tttggcttCTCTATTTAAATAGACGTCCAGTCTACGTATGCAAGTGCTCAGACTTGAATTGATGAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                          <  2:772111/135‑1 (MQ=255)                           aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                          <  2:283502/141‑1 (MQ=255)                             tgtgTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTa                                                                                                        <  2:778224/141‑1 (MQ=255)                                  tccatatTTAAGTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                   <  1:499630/138‑1 (MQ=255)                                  tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                   >  1:91683/1‑141 (MQ=255)                                     atatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACCTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGat                                                                                                <  2:580247/141‑1 (MQ=255)                                      tatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGata                                                                                               <  2:869946/141‑1 (MQ=255)                                       atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                              <  2:114949/141‑1 (MQ=255)                                       atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                              <  1:778591/141‑1 (MQ=255)                                       atTTAATCAGTCGTTCAGTATACGAATCAAAATGCTTACAACTAAATTCATCAATATTACCTCGTACTATGAAAGGACCCAATTTTATTACAGAGTTTTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                              <  1:234234/141‑1 (MQ=255)                                             ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaata                                                                                        >  1:153203/1‑141 (MQ=255)                                             ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTCAAGTTATGTGTGATaataaata                                                                                        >  1:249888/1‑141 (MQ=255)                                               aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAaataat                                                                                      >  2:654148/1‑141 (MQ=255)                                                    ttCAGTATCAGGATGCAAATGCTCATACTTTAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                 <  2:348560/141‑1 (MQ=255)                                                     tCAGTATACGGAAGCAAATGCTCAAACTTGAAATGATTAATATTACCTCGATCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAATTCAATACATGACTTAAGTTATGTGTGATAGTAAATAATGTTTGa                                                                                <  2:679206/141‑1 (MQ=255)                                                       aGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATAACTTAAGTTCTGTGTGATAATAAATAATGTTTGAcc                                                                              >  1:971952/1‑141 (MQ=255)                                                        gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             >  1:551644/1‑141 (MQ=255)                                                          ataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                           <  1:509534/141‑1 (MQ=255)                                                              ggATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACCTGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑Ct                                                                      >  2:451489/1‑141 (MQ=255)                                                                aTGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTaa                                                                    <  1:167019/141‑1 (MQ=255)                                                                aTGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTAAACTTATTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTaa                                                                    >  1:277545/1‑141 (MQ=255)                                                                    aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAACACATAATTGTACTGACACTATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATCTTTCACCACATT‑CTACTACa                                                                >  2:341479/1‑141 (MQ=255)                                                                     aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTTAGTAAAGGTTGATAGTAAATAAGGTTGGACCAAGTT‑CTAATACaa                                                               >  1:331255/1‑141 (MQ=255)                                                                     aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATTTT‑CTAATACaa                                                               >  1:523471/1‑141 (MQ=255)                                                                      aTGCTCATAGTTGAAATGATTAATATTAACGCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAat                                                              >  1:731257/1‑141 (MQ=255)                                                                       tGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTCACCATGTT‑CTAATACAata                                                             <  1:742964/141‑1 (MQ=255)                                                                           cATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCACGTT‑CTCATACAATATGCt                                                         <  2:875913/141‑1 (MQ=255)                                                                             tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACCTGACTTAAGGTATGTGTGATAATATCTAATGTTTGACCATGTT‑CTAATACATTATGCTGt                                                       >  1:264956/1‑141 (MQ=255)                                                                             tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTGATGTGTGATAATAAATAATGTTTGACAATGTT‑CTAATACAATATGCTGt                                                       >  2:900133/1‑141 (MQ=255)                                                                              aCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGta                                                      >  2:405771/1‑141 (MQ=255)                                                                                   aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATCCAATATGCTGTAATaaa                                                 >  2:368492/1‑141 (MQ=255)                                                                                   aaaTGATTAATATTACATCGTGCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATaaa                                                 >  1:510882/1‑141 (MQ=255)                                                                                                 aCCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGAtt                                   >  1:907138/1‑141 (MQ=255)                                                                                                  ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTCCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTa                                  <  2:318833/141‑1 (MQ=255)                                                                                                    tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTAcc                                <  1:654148/141‑1 (MQ=255)                                                                                                    tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATAACTTGATTAcc                                >  1:487182/1‑141 (MQ=255)                                                                                                    tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CGAATACAATGTGCTGTAATAAATCCATCACTTGATTAcc                                >  1:827909/1‑141 (MQ=255)                                                                                                    tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACC‑TGTTGCTAATACAATATGCTGTAATAGATCCATCACTTGATGAcc                                >  1:827910/1‑141 (MQ=255)                                                                                                         cAATGATTTGACCGTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCa                           >  2:363037/1‑141 (MQ=255)                                                                                                          aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCCTGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaa                          >  2:472584/1‑141 (MQ=255)                                                                                                             gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCACTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCCAAGc                       >  1:191360/1‑141 (MQ=255)                                                                                                              aTTTGACCTTCTTTTAAAACATTATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCa                      <  1:850270/141‑1 (MQ=255)                                                                                                              aTTGGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGCTTACCGTTCAAAGCa                      >  1:416867/1‑141 (MQ=255)                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAACATc                    >  1:852913/1‑141 (MQ=255)                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                    >  1:409192/1‑141 (MQ=255)                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                    >  1:617189/1‑141 (MQ=255)                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                    <  1:858344/141‑1 (MQ=255)                                                                                                                ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                    >  1:826523/1‑141 (MQ=255)                                                                                                                ttGACCTTCTTTTAAAACAGAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTACTCAATCCACCACTTGATTACTGTTCAAAGCATc                    >  2:557425/1‑141 (MQ=255)                                                                                                                ttGACCTCCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                    <  1:2119/141‑1 (MQ=255)                                                                                                                   aCCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa                 >  1:87824/1‑141 (MQ=255)                                                                                                                   aCCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa                 >  1:651424/1‑141 (MQ=255)                                                                                                                         tctcaaaaCATAATTGTACTGACAATATTTCAAGACATGACTTAAGGTATGTGTTAGTACGAATAATTTTTGACCATGTT‑CTAATACAATATGCTGAAATAAATCCATCTCTTGATTACCGTTCAAAGCATCCAATGATGc           <  2:546846/137‑1 (MQ=255)                                                                                                                           ttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCAAATGATGCaa         >  1:422188/1‑141 (MQ=255)                                                                                                                              aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      >  2:991660/1‑141 (MQ=255)                                                                                                                              aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      >  2:852825/1‑141 (MQ=255)                                                                                                                              aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      >  2:852354/1‑141 (MQ=255)                                                                                                                              aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg      >  1:105876/1‑141 (MQ=255)                                                                                                                               aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAGTAAGTCCATCACTTACTTACCGTTCAAACCCTCCAATGACGCAACTgg     >  1:427936/1‑141 (MQ=255)                                                                                                                               aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAGGCATCCAATGATGCAACTgg     >  2:49209/1‑141 (MQ=255)                                                                                                                               aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGCTGCAACTgg     >  2:245737/1‑141 (MQ=255)                                                                                                                               aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTgg     >  2:485677/1‑141 (MQ=255)                                                                                                                                  aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGCTTCCCGTTCAAAGCACCCAATGATGCAACTGGTTc  <  2:731257/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                          AACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTT‑CTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  USA300TCH1516_ALE/1416325‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑