BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	97.7%	300.5 / NA	88	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (37/48); total (38/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGG  >  USA300TCH1516_ALE/1646053‑1646326

tgtctCACGTGCAACACCATGTGGTAATGCACCTTTAGGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                        <  2:51737/137‑1 (MQ=255)

 ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                       <  1:623984/141‑1 (MQ=255)

   aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                     >  2:326454/1‑141 (MQ=255)

   aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                     >  2:874194/1‑141 (MQ=255)

   aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                     <  1:947372/141‑1 (MQ=255)

     cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGCTGCTACAATCGTATCGAATGATGGTCCATCTGCTCATAATTCTAGCATTTTGCTTTCTAATATTGACTTCAATTCTTTTTCGGTCAGGCCTCCTTTTacca                                                                                                                                   >  2:526551/1‑139 (MQ=255)

      aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                  <  2:616023/141‑1 (MQ=255)

      aCTAGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                  <  2:845648/141‑1 (MQ=255)

         ggCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTAGCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTTTTTTTCAGTCATGCCGCCTTTTACATCAGt                                                                                                                               <  2:604682/140‑1 (MQ=255)

          gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                              <  1:934202/141‑1 (MQ=255)

          gCAACACCATCTGGTAATGCCCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                              <  2:899018/141‑1 (MQ=255)

           cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTa                                                                                                                             <  1:608536/141‑1 (MQ=255)

              cacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                          <  1:700454/141‑1 (MQ=255)

              cacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                          <  2:363877/141‑1 (MQ=255)

              cacCATGTGGTAACGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGTCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                          <  1:193344/141‑1 (MQ=255)

              agccATGTGGTAATGCACCCGTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCACTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                          <  2:382322/139‑1 (MQ=255)

               acCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACACCAGTTAAAat                                                                                                                         >  1:806832/1‑141 (MQ=255)

                ccgtgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                        <  1:866510/138‑1 (MQ=255)

                      ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                                  <  1:857587/141‑1 (MQ=255)

                      ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                                  <  2:273241/141‑1 (MQ=255)

                       gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                                 <  1:77609/141‑1 (MQ=255)

                       gTAATGCACCTCCATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                                 <  1:303412/141‑1 (MQ=255)

                           tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                                                                             <  2:845483/141‑1 (MQ=255)

                            gCACCTCTAGGACCACATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                                                                            <  2:33532/141‑1 (MQ=255)

                              aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtc                                                                                                          >  1:788968/1‑141 (MQ=255)

                               ccTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtca                                                                                                         <  2:138856/141‑1 (MQ=255)

                               ccTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtca                                                                                                         <  1:984770/141‑1 (MQ=255)

                               ccTCTATGACCAGATGCTACAATCGTATCGAAAGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtct                                                                                                         >  1:713146/1‑140 (MQ=255)

                                  ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATCTGTTtcatca                                                                                                      >  2:708632/1‑141 (MQ=255)

                                        ccAGATGCTACATTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAata                                                                                                <  2:26494/141‑1 (MQ=255)

                                        ccAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAata                                                                                                <  1:487675/141‑1 (MQ=255)

                                                       gTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGa                                                                                >  2:182850/1‑141 (MQ=255)

                                                         aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAAt                                                                              >  2:321078/1‑141 (MQ=255)

                                                           cGAATGATGGTCCATCTGCTCATAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCGTCAACAATGTTAGCGGC‑TGGTTGAtttt                                                                            >  2:682661/1‑140 (MQ=255)

                                                              atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCACCAATATTAGCTGC‑TTTTTGAATTagag                                                                         >  1:948676/1‑141 (MQ=255)

                                                              atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGATTTaaac                                                                         >  2:928776/1‑140 (MQ=255)

                                                               tgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGc                                                                        >  1:370408/1‑141 (MQ=255)

                                                                     tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTc                                                                  >  1:818549/1‑141 (MQ=255)

                                                                     tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTAC‑TTTTTGAATTAAAGCAATTTc                                                                  >  1:545902/1‑141 (MQ=255)

                                                                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTct                                                                 >  2:444817/1‑140 (MQ=255)

                                                                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCACTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCg                                                                 >  2:116784/1‑141 (MQ=255)

                                                                      ccATCTGCTCCTAAGTCTAGCATTTTGCTTTCTATAAGTGCCTTTACTTCTTCGTCAGGCATCCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCg                                                                 >  2:453025/1‑141 (MQ=255)

                                                                          cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAg                                                             >  1:612792/1‑141 (MQ=255)

                                                                           tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCCGc                                                            >  1:288751/1‑141 (MQ=255)

                                                                            gccccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCCTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCa                                                           <  1:87194/138‑1 (MQ=255)

                                                                               ccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCt                                                        <  1:874194/141‑1 (MQ=255)

                                                                               ccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCt                                                        <  1:326454/141‑1 (MQ=255)

                                                                                 tcaTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAATAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                      <  1:830841/139‑1 (MQ=255)

                                                                                 tAATTCTATCCTTTTGCTTTCTCATCTTGCCTTCCATTATTTTTGAGTCGTGTCTCTTTTTACGGCCCCTAAAATTTTTTCGGAGGTTGCATCAACAATATGATCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                      <  2:477094/141‑1 (MQ=255)

                                                                                   attgtcgCAGTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGa                                                    <  2:823075/135‑1 (MQ=255)

                                                                                   aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGa                                                    >  2:13647/1‑141 (MQ=255)

                                                                                   aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTAAGCATCTTTGa                                                    >  2:61485/1‑141 (MQ=255)

                                                                                        aGCATTTTGCTTTCTAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                               <  1:275416/141‑1 (MQ=255)

                                                                                        aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                               >  2:527279/1‑141 (MQ=255)

                                                                                         gCATTTTGCTTTCTACTATTGCCTTTAATTCTATTTCAGAAGTTGCTGCTTTTACAACAGTTAAAATATATTCATATGTTTTATCCACAATATTAGCTGC‑TTTTTGAATTAAAGTAATTTCGTCTGGATTTATGACGtctc                                              <  2:449601/141‑1 (MQ=255)

                                                                                         ccATTTTGTTTTCTAATATTGCCTTTAATTCTTTTTCATTCATTCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTATCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                              <  2:765555/140‑1 (MQ=255)

                                                                                          cATTTTGCTTTCTAATATTTCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                             <  1:420035/141‑1 (MQ=255)

                                                                                          cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                             <  2:119290/141‑1 (MQ=255)

                                                                                          cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                             <  2:64048/141‑1 (MQ=255)

                                                                                          cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTCCGTCAGCATCTTTGACGTctct                                             <  2:727282/141‑1 (MQ=255)

                                                                                          cATTTTGCTTTCTAATATTGCCTTTAACTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                             <  2:277933/141‑1 (MQ=255)

                                                                                           aTTTTGCTTTCTAATATTGCCTTTACTTCTTTTTCCGTCATGCCTCCTTTTACAACAGTTAAAATTTATTCATATGTTTCATCAACAATATTAGCTTC‑TTTTGAAATTAAAGCACTTTAGTAAGCATCTTTGACGtctttt                                            >  2:277290/1‑138 (MQ=255)

                                                                                           aTTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                            >  2:405516/1‑141 (MQ=255)

                                                                                           aTTTTGCTTTCTAATATTGCCTTTAATTATTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGAAATTTGGTCAGCATCTTTGACGTCTCTa                                            >  2:500898/1‑141 (MQ=255)

                                                                                                 cTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                      <  1:319099/141‑1 (MQ=255)

                                                                                                 cTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                      <  1:372651/141‑1 (MQ=255)

                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCCACAGTATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                     <  2:248961/141‑1 (MQ=255)

                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                     <  1:981464/141‑1 (MQ=255)

                                                                                                  tttCTAATATTCCCCTTACTGCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCCTTGACGTCTCTAATTTTAt                                     <  2:89543/141‑1 (MQ=255)

                                                                                                    tCTATTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                                   <  2:868842/141‑1 (MQ=255)

                                                                                                     cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGATTTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                  >  1:673902/1‑141 (MQ=255)

                                                                                                     cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTTTCTa                                  >  2:48717/1‑141 (MQ=255)

                                                                                                     cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                  >  1:192364/1‑141 (MQ=255)

                                                                                                     cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                  >  1:630890/1‑141 (MQ=255)

                                                                                                     cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAACTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                  >  2:511774/1‑141 (MQ=255)

                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                           <  2:788968/141‑1 (MQ=255)

                                                                                                             gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTTACGTCTCTAATTTTATTTACAGTAtt                           >  2:74828/1‑141 (MQ=255)

                                                                                                             gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTCCAGTAtt                           >  2:977792/1‑141 (MQ=255)

                                                                                                             gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                           >  2:58792/1‑141 (MQ=255)

                                                                                                                    aTTGTTTTTCAGGCATGCTTTCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                   <  2:491119/141‑1 (MQ=255)

                                                                                                                    aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                   <  1:522879/141‑1 (MQ=255)

                                                                                                                         ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt              >  1:689606/1‑141 (MQ=255)

                                                                                                                         ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt              >  1:198427/1‑141 (MQ=255)

                                                                                                                            tCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAt           <  2:872885/141‑1 (MQ=255)

                                                                                                                             cAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg          >  2:757521/1‑141 (MQ=255)

                                                                                                                                  aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat      >  1:685025/1‑140 (MQ=255)

                                                                                                                                  aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat      <  2:685025/140‑1 (MQ=255)

                                                                                                                                    gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg   <  1:807200/141‑1 (MQ=255)

                                                                                                                                     cctcctTTGACAACAGTTATAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACgg  <  2:437148/141‑1 (MQ=255)

TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGC‑TTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGG  >  USA300TCH1516_ALE/1646053‑1646326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`