| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,744,473 | C→T | A67T (GCT→ACT) | apt ← | Adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,744,473 | 0 | C | T | 98.9% | 259.0 / NA | 88 | A67T (GCT→ACT) | apt | Adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (50/36); minor base G (1/0); total (51/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCC > USA300TCH1516_ALE/1744338‑1744609 | gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAg < 2:735290/141‑1 (MQ=255)gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAg > 1:847621/1‑141 (MQ=255)gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAg > 1:456350/1‑141 (MQ=255) aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg > 1:414454/1‑141 (MQ=255) ccTGGTTTAGTTGCCTCTTTGGGCGTTGGTAACACATTTGTACCCTACTCGAGGTCATACTCATAACGAATGACTTCACGCGGT‑TAGCCCCCTCCGTTTCTAACCGGTGCAAAGCCAATGCCCACTGAAGAAGTTACAggg < 2:767049/141‑2 (MQ=255) ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATTACGGGTGGCTGGGCGAGGT‑CTTTTGCCTGCTTGTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGa < 1:233965/141‑1 (MQ=255) tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGCCa > 2:194085/1‑141 (MQ=255) tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa < 1:905432/141‑1 (MQ=255) tAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGCCAAACTCATAGCGAAT‑AATTCAATATTTGAAGTTCCACTTTCTGCGAATAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa < 1:727832/141‑1 (MQ=255) aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCCTTGAATAAGTTCCAGGACAGCCAATg > 1:328091/1‑141 (MQ=255) ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCCATCCCCATTGAATACGTTACAGGCCAGCCAATGa > 2:388496/1‑141 (MQ=255) ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGCCAGCCAATGa > 2:824569/1‑141 (MQ=255) ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGa > 2:981034/1‑141 (MQ=255) aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAGAGCCAATCCCCATTGAATAAGTTACAGGACACCAAATAATaaa > 2:692951/1‑141 (MQ=255) aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGCCAGCCAATGATaga > 2:769048/1‑139 (MQ=255) aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATaaa < 2:845975/141‑1 (MQ=255) tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGcc > 2:775315/1‑141 (MQ=255) tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCGTAGGATAAGTTACAGGACAGCCAATGATAAAGCCAc > 1:927716/1‑141 (MQ=255) tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc > 2:552400/1‑141 (MQ=255) tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc > 1:967502/1‑141 (MQ=255) tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc > 1:872839/1‑141 (MQ=255) tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTc < 1:325185/141‑1 (MQ=255) tGTAAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTc < 2:302190/141‑1 (MQ=255) tAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAgg > 1:573104/1‑141 (MQ=255) aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGGTc > 2:271633/1‑141 (MQ=255) aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTTTTTTCTAACTGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCCCTTCAGGTcc > 1:761590/1‑141 (MQ=255) aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc > 2:274992/1‑141 (MQ=255) aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc > 1:399190/1‑141 (MQ=255) tGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAAc < 1:282860/141‑1 (MQ=255) gTACCATATTCTAGGTCATACTCATAACGAATGCCTTCACGAGGT‑AATTTCCCTTCTATTCTAACAGGTGCAAAGCCAATCCCCATTGAATATGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg > 2:983953/1‑141 (MQ=255) gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACCGAAAACGAAAGAGCGACGCTCTGCAAGTCCTACAACg > 2:938443/1‑141 (MQ=255) gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGCCCTACCACg > 2:967611/1‑141 (MQ=255) gTACCATATTCTAGGTCATACTCATAACGAATGACTTAACGAGGT‑AATTTCCCTACTTGTCTAACAGATGCAAAGCCAATCTTCATGGCATCATTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTGCTACAACg > 2:470245/1‑141 (MQ=255) tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa > 1:681140/1‑141 (MQ=255) aCCATATTCTAGGTCATACTCGTAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATAATAAAGCCACGCGCTTCAGGTCCTACAACGat > 2:261609/1‑141 (MQ=255) aCCATATTCTAGGTCATACTCGTAACGAATGACTTCACGAGGT‑AATTTCACTTCTTTTCTAACAGGTGCAAAGCCAATCCCAATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACAat > 2:261579/1‑141 (MQ=255) aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCCTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGaa > 2:132034/1‑140 (MQ=255) cATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTAATTTCTAACAGGTGCAAAGCCAATCCCCATTGAATACGTCATAGGACAGCCAATGATAAAGCCACGCTCTTCAGGTCCTACAACGatat > 2:349413/1‑141 (MQ=255) ttCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc < 2:456350/141‑1 (MQ=255) ttCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc < 1:259188/141‑1 (MQ=255) cTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGCTATCAACAt > 1:43045/1‑141 (MQ=255) cTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt > 1:592280/1‑141 (MQ=255) aGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCCTTGAATAAGTTACCGGACAGCCAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtcc > 2:311845/1‑140 (MQ=255) gTCATACTCATCACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTCACAGGTGCAACGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACTACGATATCAACATctct < 2:372709/141‑1 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCACCATCTCTg > 2:229411/1‑141 (MQ=255) aCTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtt < 1:9248/141‑1 (MQ=255) cgcATCACGACTGACTTCACGAGGT‑AATTTCCCTGCTGTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAGTTTACAGGACAGCCAATGATATAGCCTCGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt < 2:316777/139‑1 (MQ=255) cTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt > 1:768660/1‑141 (MQ=255) cTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCCACAACGATATCAACATCTCTGTCttt < 2:617219/141‑1 (MQ=255) cTCATAACGAATGACTGCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt > 1:510270/1‑141 (MQ=255) aCGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTa > 2:611966/1‑141 (MQ=255) cGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt < 1:145089/141‑1 (MQ=255) aTGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGACTAAGTTACAGGACAGCCAATCATAAAGCCACGCGCTTCAGATCCTACAACGATATCAAACTCTCTGTCTTTTCCGTAttgt > 2:345409/1‑139 (MQ=255) aTGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTTTTCt > 1:490992/1‑141 (MQ=255) aTGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt > 1:57636/1‑141 (MQ=255) aTGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTCCAGGTCCGACAACGATATCACAATCTCTGTCTTTTTCCTATTCt > 1:182636/1‑141 (MQ=255) ttCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAAGCATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAt < 1:309736/141‑1 (MQ=255) cACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt < 1:540908/141‑1 (MQ=255) cACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt < 1:596001/141‑1 (MQ=255) aCGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtttt < 2:662761/141‑1 (MQ=255) cGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGTATCTTTCACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 1:28718/141‑1 (MQ=255) cGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 2:872839/141‑1 (MQ=255) cGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 2:927716/141‑1 (MQ=255) cGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 2:967502/141‑1 (MQ=255) cGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 1:552400/141‑1 (MQ=255) t‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt < 1:598660/141‑1 (MQ=255) t‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt < 1:99641/141‑1 (MQ=255) t‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt < 1:177907/141‑1 (MQ=255) t‑AATTTACCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt > 2:860604/1‑141 (MQ=255) t‑AATTTACCTTCTGTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGACATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt > 2:860693/1‑141 (MQ=255) aaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGGCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATGTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt > 2:500102/1‑141 (MQ=255) aaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt < 1:844359/141‑1 (MQ=255) aaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt < 2:454539/141‑1 (MQ=255) cttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCat < 2:573104/141‑1 (MQ=255) ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATg < 1:62850/141‑1 (MQ=255) tAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCtt > 2:825065/1‑141 (MQ=255) aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCTTTGCCATAGGCTTc > 2:340372/1‑141 (MQ=255) acggcggCAAAGCCAAGCCCCAGTGAATAAGTTACAGGACACCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTTTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa < 2:79911/135‑1 (MQ=255) aGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc < 2:414454/141‑1 (MQ=255) tGCAAAGCCAATCCCCATTGAATAAGGTACAGGACAGCAAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATAGAAACATCTCTGTCTTTTGCGTATCCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAta > 2:909306/1‑140 (MQ=255) gCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTa < 2:735913/141‑1 (MQ=255) gCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt < 2:759865/141‑1 (MQ=255) cAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCCCCATTATCCATaa > 1:552267/1‑141 (MQ=255) cAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa > 1:941287/1‑141 (MQ=255) aTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATCGCCATATGCTTCACCATTATCCTTCATGgtagta > 1:961720/1‑141 (MQ=255) aTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagta > 2:470939/1‑141 (MQ=255) gAATATGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAata < 2:3137/141‑1 (MQ=255) tAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCTTATTCTAAAATTTTATCTGTTGCTTAGCCAGACGCTTCACCATTATTCATAATTGTAGTAatattc > 1:91044/1‑139 (MQ=255) | GACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGT‑AATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCC > USA300TCH1516_ALE/1744338‑1744609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |