BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,358,585	G→C	D111E (GAC→GAG)	rpoA_1 ←	DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,358,585	0	G	C	100.0%	393.9 / NA	115	D111E (GAC→GAG)	rpoA_1	DNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (53/62); total (53/62)

Only 100 of 115 total aligned reads displayed.

CTAGTATTATTTTGTTCTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTG  >  USA300TCH1516_ALE/2358448‑2358721

cTAGTATTATTTTGTTCTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCg                                                                                                                                       <  2:853140/141‑1 (MQ=255)

       tattTTGTTCTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTg                                                                                                                                >  1:773289/1‑141 (MQ=255)

          tttGTTCTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACAGCACTATCATGTGTAATCTCGCTTGCTGttt                                                                                                                             >  2:628655/1‑140 (MQ=255)

                cTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGc                                                                                                                       >  1:484066/1‑141 (MQ=255)

                cTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGc                                                                                                                       >  2:848519/1‑141 (MQ=255)

                cTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCAAATTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATAACTATAATGTGTAATCTCGCTTGCTGTTACTTCGc                                                                                                                       >  1:781506/1‑141 (MQ=255)

                  gCTGATGCGTAACCTCGACCCTTGTTAGCAATAAGACAAATTTTTAAGTGACCACCTTTTTAGACTTTGGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCAGGTGTAATCTCGCTTGCTGTTACTTCGCCt                                                                                                                     >  2:453042/1‑141 (MQ=255)

                  gCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGTTTGCTGTTACTTCGCCt                                                                                                                     <  2:517201/141‑1 (MQ=255)

                  gCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGTTTGCTGTTACTTCGCCt                                                                                                                     <  2:329373/141‑1 (MQ=255)

                   cTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCCGTTACTTCGCCtt                                                                                                                    <  2:934584/141‑1 (MQ=255)

                    tAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTtc                                                                                                                   <  1:762589/141‑1 (MQ=255)

                    tAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTtc                                                                                                                   <  1:281348/141‑1 (MQ=255)

                    tAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTtc                                                                                                                   <  1:883445/141‑1 (MQ=255)

                     aaTGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGTTACTGTTGCAATTTTAAGCTCTGGGTTTAAAGTTTGAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTcca                                                                                                                  >  2:649027/1‑141 (MQ=255)

                     aaTGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCttaa                                                                                                                  >  2:494912/1‑139 (MQ=255)

                      aTGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTGTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTtcat                                                                                                                 >  2:265122/1‑141 (MQ=255)

                        gCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTtcatca                                                                                                               >  1:35153/1‑141 (MQ=255)

                        gCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTtcatca                                                                                                               >  1:905493/1‑141 (MQ=255)

                         cGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATATGTAATCTCGCTTGCTGTTACTTCGCCTGCCTCAc                                                                                                              >  1:121229/1‑141 (MQ=255)

                            aaCCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGGTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCttcttcccata                                                                                                           >  1:402936/1‑136 (MQ=255)

                             aCCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTAc                                                                                                          >  1:694833/1‑141 (MQ=255)

                             aCCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTAc                                                                                                          <  1:469743/141‑1 (MQ=255)

                               ctctACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACAt                                                                                                        >  1:214083/1‑141 (MQ=255)

                               ctctACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACAt                                                                                                        >  1:430012/1‑141 (MQ=255)

                                    cccTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAAtt                                                                                                   <  2:230748/141‑1 (MQ=255)

                                       ttGTTAGCAACTAGACGAATTTTTATGTTACCACCTTTAGATACTGTTGCAATTATAAGCTCTTGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCCATTTCt                                                                                                <  2:48350/141‑1 (MQ=255)

                                       ttGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCATTCTTATGTTTAATCTCGCTTGCTGTGACTTCGCCTTCATCACGTTCATCAATTTCt                                                                                                <  2:956701/141‑1 (MQ=255)

                                       ttGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCt                                                                                                <  2:350629/141‑1 (MQ=255)

                                       ttGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCt                                                                                                <  2:840442/141‑1 (MQ=255)

                                            aGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTCCATCAATTTCTAAAGt                                                                                           >  2:934960/1‑141 (MQ=255)

                                                  tAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCCCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATAACGTACATCAATTTCTAAAGTTTTAtc                                                                                     >  2:787217/1‑141 (MQ=255)

                                                  tAGACGAATTTTGAAGTGAGCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTACACTGGCTAGCTGTCGGGTTGCCTTCATAACGTACATCAATTTCTAAAGTTTTAtc                                                                                     >  2:953082/1‑141 (MQ=255)

                                                   aGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATTTTTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTAtct                                                                                    <  1:136171/141‑1 (MQ=255)

                                                    gACGAATTTTTAAGTGACCACCTTTAGTTACTGTTTCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTGACTTCGCCTTCATCACGTACATCAATTTCTACAGTTTTAtctt                                                                                   <  2:958361/141‑1 (MQ=255)

                                                    gACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTAtctt                                                                                   <  1:395600/141‑1 (MQ=255)

                                                            tttAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCagag                                                                           >  1:692301/1‑141 (MQ=255)

                                                             ttAAGTGACCGCCTTTCGATCCTGTTGCAAGTTTAAGCTCTGGTTTTAAAAGTTCAACATCACGATCATGTGTAAGCTCTCTTGCTATTACTTCGCCTTCATCTCGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGt                                                                          <  2:388745/141‑1 (MQ=255)

                                                              tAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTa                                                                         <  1:155331/141‑1 (MQ=255)

                                                                  tcagcaccTTTAGATACTGTTGCAATTTTAAGTTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAAtt                                                                     <  2:29331/137‑1 (MQ=255)

                                                                  tGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAAtt                                                                     >  1:99008/1‑141 (MQ=255)

                                                                  tGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAAtt                                                                     <  1:279245/141‑1 (MQ=255)

                                                                   gACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAAttt                                                                    >  2:435511/1‑141 (MQ=255)

                                                                        ccTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAAt                                                               >  1:395686/1‑141 (MQ=255)

                                                                         cTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATg                                                              <  2:161435/141‑1 (MQ=255)

                                                                         cTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTCTTATCTTCTTCAGAGTAAATTTTCAATg                                                              >  2:241074/1‑141 (MQ=255)

                                                                          tttAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGc                                                             <  1:535453/141‑1 (MQ=255)

                                                                          tttAGATACTGTTGCAATTTTAAGCTCTGGCTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGc                                                             <  1:514225/141‑1 (MQ=255)

                                                                           ttAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCt                                                            <  2:35153/141‑1 (MQ=255)

                                                                           ttAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCt                                                            >  1:519280/1‑141 (MQ=255)

                                                                           ttAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGACTTCATCACGTACATCAATTGCTCAAGTTTTATCTTCTTCCGCGAAAATTTTCACTGCt                                                            >  1:426297/1‑141 (MQ=255)

                                                                             ataTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCACTTCCTCAAGTATCCTCTCCATCCGACCAAATCTTCAAACCTaa                                                          <  2:481231/139‑1 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAAtt                                                        <  2:503663/141‑1 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAAtt                                                        <  2:565951/141‑1 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAAtt                                                        <  2:805765/141‑1 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAAtt                                                        >  1:119333/1‑141 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAAtt                                                        <  2:197971/141‑1 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAAGGCGAAtt                                                        >  1:126860/1‑141 (MQ=255)

                                                                               aTACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTAATGCCGTGTAAATTTTCAATGCTAGtt                                                        >  1:923349/1‑141 (MQ=255)

                                                                                  ccgTTGCAATTTTTAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCTATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGtt                                                     <  2:618959/139‑1 (MQ=255)

                                                                                  cTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGtt                                                     <  2:484066/141‑1 (MQ=255)

                                                                                  cTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGtt                                                     <  1:848519/141‑1 (MQ=255)

                                                                                      tGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTACTTGTTTAAt                                                 >  2:252538/1‑141 (MQ=255)

                                                                                      tGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAAt                                                 >  2:899902/1‑141 (MQ=255)

                                                                                       tcAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATg                                                <  2:645506/140‑1 (MQ=255)

                                                                                       gCACTTTTAAGCTCTGGGTTTAAAATTTCAACATCAAAATGATTTGTAATCTCGTTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTCAATTTTCAATGCTAATTGTTTAATg                                                <  2:698115/141‑1 (MQ=255)

                                                                                       gCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATg                                                <  2:468788/141‑1 (MQ=255)

                                                                                       gCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATg                                                <  1:614917/141‑1 (MQ=255)

                                                                                        cAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGt                                               <  1:899902/141‑1 (MQ=255)

                                                                                         aaTTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGtt                                              >  1:380488/1‑141 (MQ=255)

                                                                                         aaTTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACAGCAATTTCTAAAGTTTTATCTTCTTCACAGTAAATTTTCAATGCTAATCGTTTTATgct                                              >  1:300581/1‑139 (MQ=255)

                                                                                          aTTTTAAGCTCTGGGTTTAAAATTTCAACCTCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATACTAATTGTTTCATGttt                                             >  1:947143/1‑140 (MQ=255)

                                                                                            tttAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATGTTAAATGCGAAGTGTTTAATGttaaa                                           >  2:837788/1‑138 (MQ=255)

                                                                                              tAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCataa                                         >  1:302204/1‑141 (MQ=255)

                                                                                                aGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTTTTTAATGTTCataata                                       >  2:79431/1‑141 (MQ=255)

                                                                                                aGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCataata                                       <  1:294331/141‑1 (MQ=255)

                                                                                                aGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGCTCatatta                                       >  2:861376/1‑141 (MQ=255)

                                                                                                 gCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTCATGTTCAtaataa                                      >  1:406540/1‑141 (MQ=255)

                                                                                                 gCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCAtaataa                                      >  1:44272/1‑141 (MQ=255)

                                                                                                 gCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCAtaataa                                      <  2:629243/141‑1 (MQ=255)

                                                                                                  ctctGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATaataat                                     <  1:435511/141‑1 (MQ=255)

                                                                                                  ctctGGGTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATaataat                                     <  2:688004/141‑1 (MQ=255)

                                                                                                      gggTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATTCTAATTGTTTAATGTTCATAATAATTGTa                                 >  1:926804/1‑141 (MQ=255)

                                                                                                      gggTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTa                                 >  1:959088/1‑141 (MQ=255)

                                                                                                       ggTTTAAAATTTCAACATTACTATCATGTGTAAGCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATTCTAATTGTTTAATGTTCATAATAATTGTAg                                <  2:233525/141‑1 (MQ=255)

                                                                                                       ggTTTAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAg                                <  1:169044/141‑1 (MQ=255)

                                                                                                          ttAAAATTTCAACATCACTATCATGTTTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGaaa                             <  2:694833/141‑1 (MQ=255)

                                                                                                          ttAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTTCTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGaaa                             <  2:985765/141‑1 (MQ=255)

                                                                                                          ttAAAATTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGaaa                             <  1:70328/141‑1 (MQ=255)

                                                                                                           tAAAATTTCAACATCACTATCATGTGTAATCTGGCTTGCGGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCCGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTACAAAc                            >  2:371835/1‑141 (MQ=255)

                                                                                                            aaaaTTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACa                           >  1:564713/1‑141 (MQ=255)

                                                                                                            aaaaTTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTCTTGAGTAAATTTTCAATGCTAGTTGTTTAATGTTCATAATAATTGTAGAAAcg                           >  1:28237/1‑140 (MQ=255)

                                                                                                             aaaTTTCAACATCACTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACAt                          >  2:392101/1‑141 (MQ=255)

                                                                                                               ttttCTACATCACTATCACGTGTAATCTCGCTGGCTGTTACTTCGCCTTCATCACGTACATCAAGTTCGAAAGTTCTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATGATAATTGTAGAAACATCt                        <  2:846121/140‑1 (MQ=255)

                                                                                                                           ctgtCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTg            <  2:236439/138‑1 (MQ=255)

                                                                                                                           cTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCCCGTACCTCCATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTg            <  2:403750/141‑1 (MQ=255)

                                                                                                                           cTATCATGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTg            <  2:77400/141‑1 (MQ=255)

                                                                                                                             aTCATGTGTAATCTCGCATGGTGTTACTTCGCCATCATCACGTACATCAATTTCTAAGGTTTTATCTTCTTCTGAGTAAATTTTGACTGCTAATTGTTTTATATTTGTAGTAATTATAGAATAATCTTTAATTAAATtctc          >  2:459088/1‑141 (MQ=255)

                                                                                                                                aTGTGTAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCtcc       >  1:462742/1‑139 (MQ=255)

                                                                                                                                  gtgtAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTActg     >  1:793161/1‑141 (MQ=255)

                                                                                                                                     tAATCTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTActgctg  >  1:310033/1‑141 (MQ=255)

CTAGTATTATTTTGTTCTGCTAATGCGTAACCTCTACCCTTGTTAGCAACTAGACGAATTTTTAAGTGACCACCTTTAGATACTGTTGCAATTTTAAGCTCTGGGTTTAAAATTTCAACATCACTATCATGTGTAATGTCGCTTGCTGTTACTTCGCCTTCATCACGTACATCAATTTCTAAAGTTTTATCTTCTTCAGAGTAAATTTTCAATGCTAATTGTTTAATGTTCATAATAATTGTAGAAACATCTTCAACTACATTGTCTACTGCTG  >  USA300TCH1516_ALE/2358448‑2358721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`