Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	584,121	G→T	intergenic (+205/‑67)	rplA → / → rplJ	50S ribosomal protein L1/50S ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	584,121	0	G	T	100.0%	225.8 / NA	75	intergenic (+205/‑67)	rplA/rplJ	50S ribosomal protein L1/50S ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (49/26);  total (49/26)

TAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTT  >  USA300TCH1516_ALE/583985‑584256                                                                                                                                         |                                                                                                                                        tAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGAAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAACTAATTTTTGTCTTggg                                                                                                                                     >  2:898634/1‑141 (MQ=255) tAATTTCCAAGGTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCATTggg                                                                                                                                     >  2:871674/1‑141 (MQ=255)  aaTTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGt                                                                                                                                    >  1:577919/1‑141 (MQ=255)   aTTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTa                                                                                                                                   >  1:785279/1‑141 (MQ=255)    tttCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGAACGTAGGAGTTATTTATAACTTAAAATTTATCATGCCGAGGCTAAAATTGACTTGCACGTGATGATCTATGGTCTTTAAAGCCATTTTTGTCTTTGGTAg                                                                                                                                  >  2:520001/1‑141 (MQ=255)      tCCAAGTTATATTACTTATTGTTATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                <  1:597799/141‑1 (MQ=255)      tCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTCGGGGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                <  1:819134/141‑1 (MQ=255)      tCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                <  1:662312/141‑1 (MQ=255)      tCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                <  1:240885/141‑1 (MQ=255)      tCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                <  1:252280/141‑1 (MQ=255)       ccAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaaa                                                                                                                               >  2:475867/1‑141 (MQ=255)       ccAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTCTGCCTTGGGTAGaaa                                                                                                                               >  1:204735/1‑141 (MQ=255)        cAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAg                                                                                                                              >  2:614795/1‑141 (MQ=255)          aGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTGGGGTAGAAAGTg                                                                                                                            >  1:379278/1‑141 (MQ=255)              atatTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttt                                                                                                                        >  1:934345/1‑141 (MQ=255)              atatTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTAATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttt                                                                                                                        >  1:776305/1‑141 (MQ=255)               tatTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtttt                                                                                                                       >  1:330580/1‑141 (MQ=255)                            tattattTTACCAAAGCCAGTAGGAGTTATTTATAACTTAACATCTCTCCTGGCGAGGCTAAAATTGACTTGCACGTGCTTATCTATGTTGTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTa                                                                                                          <  2:648536/140‑1 (MQ=255)                            gATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTa                                                                                                          <  1:452387/141‑1 (MQ=255)                            gATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTa                                                                                                          <  1:186660/141‑1 (MQ=255)                            gATTAGTTTACCTAAGACAGTAGGAGTTCTTTATAACTTAAACTTTACCCTGCCGCGGCTAAAATTGACTTGAACGTGAGGATCTAGGTTCTTTCAAGCACTTTTGGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTa                                                                                                          <  1:226806/141‑1 (MQ=255)                                  tttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaaa                                                                                                    >  2:499021/1‑141 (MQ=255)                                  tttACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaaa                                                                                                    >  2:59885/1‑141 (MQ=255)                                      ccTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAc                                                                                                >  1:572516/1‑141 (MQ=255)                                        tAAGACAGTTGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAcaa                                                                                              <  2:464043/141‑3 (MQ=255)                                        tAAGACAATAGGAGATATATAAAACAAAAAATAAAACCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCATTTTTTGCCTTGGGTAGAAAGTTCTTTTTTAAAAAAAAAAAAAAAAAGCACCa                                                                                              <  2:998438/141‑1 (MQ=255)                                             cAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGAACCAAAAAt                                                                                         >  2:806136/1‑141 (MQ=255)                                              aGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAtt                                                                                        >  1:200232/1‑141 (MQ=255)                                              aGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAtt                                                                                        <  2:785279/141‑1 (MQ=255)                                                 aGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaa                                                                                     >  2:173450/1‑141 (MQ=255)                                                 aGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAGTTaa                                                                                     >  2:271635/1‑141 (MQ=255)                                                 aGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAATGCTTTTTTTATTAATTTTCAAACACGCACCAAAAATGTaa                                                                                     >  2:629056/1‑141 (MQ=255)                                                   gAGTTATTTGTGACTTAAAAGGGATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGGTCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAAt                                                                                   >  2:752657/1‑141 (MQ=255)                                                   gAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAAt                                                                                   >  2:363997/1‑141 (MQ=255)                                                   gAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAAt                                                                                   >  2:733807/1‑141 (MQ=255)                                                           tataACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtgt                                                                           >  2:814133/1‑141 (MQ=255)                                                           tataACTTAAAATTTATCATGGCGAGGCTAAAATTGACTTGAACGTGATGAGCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAACGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtgt                                                                           >  2:706875/1‑141 (MQ=255)                                                                  tAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATg                                                                    <  2:330580/141‑1 (MQ=255)                                                                     aaTTTATCCTTCCGAGGCTAAAATTGACTAGAACGTGATGATCTATGATCGTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCt                                                                 >  2:731751/1‑141 (MQ=255)                                                                     aaTTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTTATTTTAAAAAAAGCACCAAAAATTTAAATTGAGGTGTATGAATGTCt                                                                 >  2:128665/1‑141 (MQ=255)                                                                      aTTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCtt                                                                >  2:736343/1‑140 (MQ=255)                                                                      aTTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTg                                                                <  1:614795/141‑1 (MQ=255)                                                                         tATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTa                                                             <  2:490066/141‑1 (MQ=255)                                                                          aTCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCCCCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTAt                                                            >  1:926696/1‑141 (MQ=255)                                                                          aTCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCGGAATGTATGCTAt                                                            >  1:831208/1‑141 (MQ=255)                                                                          aTCCTGCCGAGGCTAAAATAGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTAt                                                            >  1:593973/1‑141 (MQ=255)                                                                           tCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                           >  1:855858/1‑141 (MQ=255)                                                                                 cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTATAGAAAGCACCAAAACCTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGaa                                                     >  1:875227/1‑141 (MQ=255)                                                                                 cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGaa                                                     >  2:87574/1‑141 (MQ=255)                                                                                  gAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAg                                                    <  2:156303/141‑1 (MQ=255)                                                                                    ggCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                                  <  2:917407/141‑1 (MQ=255)                                                                                    ggCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                                  <  1:190689/141‑1 (MQ=255)                                                                                    ggCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAAGGTCTGCTATCATTGAAGCt                                                  >  1:296460/1‑141 (MQ=255)                                                                                    ggCTAAAATTGACTTGAACGTGAGGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                                  <  2:667022/141‑1 (MQ=255)                                                                                      cTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGCAGCTaa                                                >  2:503503/1‑141 (MQ=255)                                                                                      cTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaa                                                >  1:175885/1‑141 (MQ=255)                                                                                      cTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaa                                                >  1:347398/1‑141 (MQ=255)                                                                                      cTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGAGTCTGAATGTCTGCTATCATTGAAGCTaa                                                >  2:788737/1‑141 (MQ=255)                                                                                        aaaaTTTACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaaaa                                              <  2:265828/141‑1 (MQ=255)                                                                                               aattGAATGTGATGATCTATGATCTTTCAACGACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACt                                       <  2:72588/139‑1 (MQ=255)                                                                                                cTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTa                                      >  1:407418/1‑141 (MQ=255)                                                                                                           tgatctatgatctTTCAAGCACTTTTTTTTTTTTTTTTAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAGCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAAt                           >  2:760913/1‑141 (MQ=255)                                                                                                           tgatctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAAt                           >  2:41491/1‑141 (MQ=255)                                                                                                                 ttggtctTTCAAGCACTTTTTGCCTTGGTTAGGAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGa                     <  2:297101/137‑1 (MQ=255)                                                                                                                      ctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTAc                >  2:150234/1‑141 (MQ=255)                                                                                                                           aaGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCa           <  1:806136/141‑1 (MQ=255)                                                                                                                           aaGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCa           >  2:409343/1‑141 (MQ=255)                                                                                                                             gcgcTTTTTGCGTTGGGTAGATAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCaaa         <  1:332538/138‑1 (MQ=255)                                                                                                                             gCACTTTTTTCTTTTTGTAGAAAGTGCATTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGACTGCTATCATTGAAGCCAAAAAACTACTAGTTGATGAAATTGCTGAGGTGTAATCaaa         >  2:363124/1‑141 (MQ=255)                                                                                                                             gCACTTTATGTCTTGCGTCGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAACTTAAATGGAGGTGTCTGAATGTCCGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCaaa         <  1:1755/141‑1 (MQ=255)                                                                                                                             acattttttACCTTGAGTAGAAAGTGCTTTTTTAAAAAAATAAAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCaaa         <  2:873404/137‑1 (MQ=255)                                                                                                                                  ttttGCCTTGGGTAGAAAGTGCTTTTTTTTTTTTTTTTTAAAAAAGCACCAACAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAACAATCAACTAGTTCATGAAATTTCTGAGGTACTCTCAAATTCat    >  2:54271/1‑140 (MQ=255)                                                                                                                                   tttGCCTTGGGTAGAAAGTGCTTTTTTTTTTATTTTTAAAAAAAGCACCAAAAATTTAAATTGAGGTGTCTGAATGTCTGCTATCATTGTAGCTCAAAAACAACTAGTTGATGAAATTGCTGAGGTAGTATCAAATTCatt   >  2:837348/1‑139 (MQ=255)                                                                                                                                   tttGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGt   >  1:962498/1‑141 (MQ=255)                                                                                                                                    ttGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAGATTCAGtt  >  1:324437/1‑141 (MQ=255)                                                                                                                                         |                                                                                                                                        TAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTT  >  USA300TCH1516_ALE/583985‑584256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑