Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,002,200	T→G	M173R (ATG→AGG)	mecA →	Adapter protein MecA 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,002,200	0	T	G	98.6%	251.4 / NA	73	M173R (ATG→AGG)	mecA	Adapter protein MecA 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (23/49);  total (23/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTAT  >  USA300TCH1516_ALE/1002063‑1002333                                                                                                                                           |                                                                                                                                      atgatCTAAACGTCAAAAGTCTACAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCAGATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  2:228783/137‑1 (MQ=255) aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  2:882388/141‑1 (MQ=255) aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAAGCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  1:289295/141‑1 (MQ=255) aGGTTCTAAACGGCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  2:228689/141‑1 (MQ=255)  ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   <  1:59606/141‑1 (MQ=255)  ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   >  2:198385/1‑141 (MQ=255)  ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   <  1:129641/141‑1 (MQ=255)  ggTTCTAAACGTCAAAAGTATTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCAGATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   <  1:660447/141‑1 (MQ=255)    ttCTAAACGTAAAAAGTCTACAGCACGAAAAAATACAACAACACACATTATGAAATTTAACGATTTCGAA‑CTGTCTATGAATTAAGCCAGGTAGATCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGa                                                                                                                                 <  1:234367/141‑1 (MQ=255)      cTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATg                                                                                                                               <  2:604376/141‑1 (MQ=255)        aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                             <  1:15707/141‑1 (MQ=255)        aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                             <  1:15504/141‑1 (MQ=255)        aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAAGTAATTCGTTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTggtggt                                                                                                                             <  1:677802/141‑1 (MQ=255)        aaaCGTCAAAAGTCCTCAGCACGTAAAAATACAAGAACAACCACCGTGACATTTAACGATTTAGAAGAAGG‑GAATAATTACGCATAGCCCGGCATTCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                             <  1:233992/141‑1 (MQ=255)         aaCGTCAACAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            <  1:359107/141‑1 (MQ=255)         aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            >  2:467079/1‑141 (MQ=255)         aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            >  2:418205/1‑141 (MQ=255)         aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            >  1:920508/1‑141 (MQ=255)         aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTCAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATCTGTTTTATAGGGTTGATGGTa                                                                                                                            <  1:511651/141‑1 (MQ=255)               aaaaTTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAATTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                                                                      <  1:350915/141‑1 (MQ=255)                 aaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattat                                                                                                                    <  2:780228/141‑1 (MQ=255)                  agtccgcAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattatt                                                                                                                   <  1:212551/135‑1 (MQ=255)                  aGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGTGTTGATGGTACttattatt                                                                                                                   >  1:16374/1‑141 (MQ=255)                          gCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGta                                                                                                           >  1:99407/1‑141 (MQ=255)                                   aaTACAAGAACAATCATTGTGCAATATAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATACAATAACTACAGAGTTTGAAGATTTGATACATAGGGTTGATGGTACTTATTATTAGGCTGTATATTTTGAt                                                                                                  >  2:185666/1‑141 (MQ=255)                                               aTCATTTTGAAATTTAACGATTTAGAAGATGG‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGTAGATTTGTGTTATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                      <  2:27629/141‑1 (MQ=255)                                               aTCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                      <  1:454716/141‑1 (MQ=255)                                               aTCAGTGTGAAATTTAATGATTTAGAAGATTT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                      <  1:792611/141‑1 (MQ=255)                                                 cATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                    >  2:269375/1‑141 (MQ=255)                                                  aTTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaa                                                                                   <  1:649948/141‑1 (MQ=255)                                                  aTTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaa                                                                                   <  1:157487/141‑1 (MQ=255)                                                     gtgAAATTTAACGATTTAGAAGAGGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                                                                <  1:179450/141‑1 (MQ=255)                                                         aaTTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACTGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                            <  2:654961/141‑1 (MQ=255)                                                         aaTTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                            <  2:921868/141‑1 (MQ=255)                                                         aaTTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                            <  2:769073/141‑1 (MQ=255)                                                                          gaTTT‑TATTAATTATGCATATCATAGCAATCCAATAATTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGt                                                           <  2:636188/141‑1 (MQ=255)                                                                          gaTGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGt                                                           <  1:549914/141‑1 (MQ=255)                                                                           aTGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTc                                                          >  1:607216/1‑141 (MQ=255)                                                                           aTGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTc                                                          >  2:467942/1‑141 (MQ=255)                                                                           aTGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTAAAGtt                                                          >  2:720551/1‑140 (MQ=255)                                                                            tGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGATTGATGGTACTAATTATTATGCTGTATATTTTGATAATCATGTTGATCAAGAAGTCATTAATGATAGTTACattaa                                                         >  2:713465/1‑137 (MQ=255)                                                                             gT‑TATTAATTATGCATATCATAGCAAGCCAATAACTACAGAGTTTGAAGAGTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCaa                                                        <  2:248083/141‑1 (MQ=255)                                                                                 attaattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTg                                                     <  1:652323/141‑1 (MQ=255)                                                                                   tgattaTGCATATCATATCAATCCAATAACTACAGAGTTTGAACATTTGTTATATAGGGTTGATGGTACTTATTATTATGCCGTATATTTTGATAGTCATGTTGATCAAGAAGCCATTCATGATAGTTACAGTCAATTGCt                                                   <  2:385387/139‑1 (MQ=255)                                                                                   taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  2:87267/141‑1 (MQ=255)                                                                                   taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  1:423243/141‑1 (MQ=255)                                                                                   taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  1:817222/141‑1 (MQ=255)                                                                                   taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  2:646068/141‑1 (MQ=255)                                                                                   taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  1:432479/141‑1 (MQ=255)                                                                                      ttaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGa                                                >  1:395425/1‑141 (MQ=255)                                                                                      ttaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGa                                                >  2:557572/1‑141 (MQ=255)                                                                                         tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAAtt                                             >  2:46171/1‑141 (MQ=255)                                                                                         tGCATATCACAGCAATCCAATCACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAAtt                                             <  1:111190/141‑1 (MQ=255)                                                                                               tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTa                                       >  2:820806/1‑141 (MQ=255)                                                                                               tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTa                                       >  2:820690/1‑141 (MQ=255)                                                                                               tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATATTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTGAGGGTAGTTACAGTCATTTGCTTGATTTTGGttt                                       >  1:33247/1‑140 (MQ=255)                                                                                                 aTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATAAAGAAGTCATTAATGATAGTTACAGTCACTGGCTGGAATTGACTTATc                                     >  2:918472/1‑141 (MQ=255)                                                                                                   aGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCa                                   >  1:830631/1‑141 (MQ=255)                                                                                                     cAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAac                                 >  2:847504/1‑141 (MQ=255)                                                                                                        tCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacaga                              >  2:302371/1‑141 (MQ=255)                                                                                                          cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacagaca                            >  2:173519/1‑141 (MQ=255)                                                                                                            ttgagtACAGGGTTTGAAGTTTTGGTATATAGGGGTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAGGTCATGAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:385793/136‑1 (MQ=255)                                                                                                            aTAATTATAGCGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATTCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:346703/141‑1 (MQ=255)                                                                                                            aTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:883149/141‑1 (MQ=255)                                                                                                            aTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGATGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:850414/141‑1 (MQ=255)                                                                                                                cTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacag                      <  1:50806/141‑1 (MQ=255)                                                                                                                cTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacag                      <  1:21696/141‑1 (MQ=255)                                                                                                                atagagagTTTGAAGAGTTGGTATATATGGTTTATTGTACTTATTGTTACGCTGTATATTTTGATAGTCATTTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAACTTGCTAATCCAACTGacagaaaag                      <  2:582000/137‑1 (MQ=255)                                                                                                                atACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacag                      <  1:244511/140‑1 (MQ=255)                                                                                                                       gTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGttta               <  2:266917/141‑1 (MQ=255)                                                                                                                             aGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTaaa         <  2:415646/141‑1 (MQ=255)                                                                                                                              gATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAAt        <  2:594483/141‑1 (MQ=255)                                                                                                                                    ttATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTAt  <  1:46171/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGT‑TATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTAT  >  USA300TCH1516_ALE/1002063‑1002333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑