BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000731	25,663	Δ1 bp	100%	coding (316/333 nt)	USA300HOU_pUSA300HOUMR0031 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	25,663	0	A	.	95.5%	488.7 / 6.6	111	coding (316/333 nt)	USA300HOU_pUSA300HOUMR0031	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/4); new base . (61/45); total (61/50)
Fisher's exact test for biased strand distribution p-value = 3.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 117 total aligned reads displayed.

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAA  >  CP000731/25523‑25798

tttgtatGTTATTTACACTCGTGCTTAAAGTTACAGGATTATCACCAAGTATTGAAGGATCTTGGTTATATCAATATTCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATa                                                                                                                                         <  2:142527/140‑1 (MQ=255)

 ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaa                                                                                                                                        >  1:149987/1‑141 (MQ=255)

 ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATAAGACAAATaa                                                                                                                                        >  2:235446/1‑141 (MQ=255)

      tGTTATTTACAGTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaat                                                                                                                                   <  2:111572/141‑2 (MQ=255)

       gTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaata                                                                                                                                  >  1:375447/1‑139 (MQ=255)

          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTTTAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATAATACAAAT‑AAAAAATATAc                                                                                                                              >  1:31712/1‑141 (MQ=255)

          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATAc                                                                                                                              >  1:322719/1‑141 (MQ=255)

          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATAc                                                                                                                              >  2:57580/1‑141 (MQ=255)

            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAATGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGCAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACAt                                                                                                                            >  2:219510/1‑141 (MQ=255)

              acacTAGTGCTTAAATTTACAGGTTTCTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCt                                                                                                                          >  2:247609/1‑141 (MQ=255)

               cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGTATCTTGGTTTTCTCAATATACAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTCCAAAT‑AAAAAATACACATCTa                                                                                                                         >  1:152908/1‑141 (MQ=255)

               cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTa                                                                                                                         >  2:113431/1‑141 (MQ=255)

                acTCGTGCTTACCTTTAAAGGTTTGTCACCAAGTACTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCGTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTaa                                                                                                                        >  2:377617/1‑141 (MQ=255)

                  tAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAg                                                                                                                      <  1:119263/141‑1 (MQ=255)

                    gTGCTTAAATTTACAGGTTTGTGACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAATGGt                                                                                                                    >  2:301255/1‑141 (MQ=255)

                    gTGCTCAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGATGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGt                                                                                                                    >  2:328393/1‑141 (MQ=255)

                       cctAAATTTACAGGTTTGTCACCAAGTAATAAAGGATCTTGGTTTTCTCAATATCCAGA‑AAATTACTTTATAGTTGTAGCACTATTTCTTATGATGTTTAATTACTATACTACAAAT‑AAAAAATACACATCTAAAGGTTaa                                                                                                                 >  2:11598/3‑141 (MQ=255)

                        ttAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTGTTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACAACTCAAACTTaaa                                                                                                                >  1:275710/1‑141 (MQ=255)

                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTTTTTATTTACTATACTATAAAT‑AAAAAATATACATCTAAAGGTTAAAt                                                                                                               >  1:151244/1‑141 (MQ=255)

                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAt                                                                                                               >  1:154334/1‑141 (MQ=255)

                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAt                                                                                                               >  1:211023/1‑141 (MQ=255)

                          taaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAtt                                                                                                              <  2:67253/140‑1 (MQ=255)

                          aaaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAtt                                                                                                              <  2:268307/141‑1 (MQ=255)

                          aaaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAtt                                                                                                              <  1:232277/141‑1 (MQ=255)

                          aaaTTTACAGGTATGTCATCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAtt                                                                                                              <  2:209461/141‑1 (MQ=255)

                            aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCGCT‑TTAAGAGAACTCTCTATATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTaa                                                                                                            <  1:110869/141‑1 (MQ=255)

                            aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTaa                                                                                                            <  1:356697/141‑1 (MQ=255)

                             tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                           >  2:397374/1‑141 (MQ=255)

                                    gTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTATCATGGAAGGAATCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATAAATGAAAAAt                                                                                                    <  1:289632/141‑1 (MQ=255)

                                       tGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGa                                                                                                 >  1:256031/1‑141 (MQ=255)

                                       tGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAAAAGa                                                                                                 >  2:380864/1‑141 (MQ=255)

                                       tGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAAAAGa                                                                                                 >  2:199812/1‑141 (MQ=255)

                                          cACCAAGTATTGAAGGATCTTTGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAtt                                                                                              <  2:227834/141‑1 (MQ=255)

                                                 tATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTCCAAGT‑ACAAAATAGACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAat                                                                                       >  1:254426/1‑141 (MQ=255)

                                                  aTTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAata                                                                                      >  2:177304/1‑141 (MQ=255)

                                                    tGAAGGATCTTGTTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                    <  2:31706/141‑1 (MQ=255)

                                                    tGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                    >  1:224616/1‑141 (MQ=255)

                                                      aaGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                  <  1:229841/141‑1 (MQ=255)

                                                      aaGGATCTTGGTTTTCTCAATATCCAAA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                  <  1:346357/141‑1 (MQ=255)

                                                      aaGAATATTAGTTTTCTAAATATTCAAA‑AAAATTCTTAATAATGGTAGCAATATTTATCAAGATAGTGAATAACTATAGTACAAAT‑AAAAAATATACATCTAAAGGTAAAAATAATGAAAAATAGAATTAGAGAATATAGa                                                                                  <  2:240195/141‑1 (MQ=255)

                                                       aGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATAAGAGAATATAGaa                                                                                 >  1:325893/1‑141 (MQ=255)

                                                             ttGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGCAAAAAAGAATTAGAGAATATAGAAAACaaa                                                                           >  2:252038/1‑141 (MQ=255)

                                                              tagTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGAAGAACTATATAATATGCTGTTTAATTACTATACAACAAAT‑AAAAAATATACATATAAAGGTTAAATTAATGAAAAATAGAATTTGAGaaaaaaaaaaaaaaaa                                                                          <  2:370594/139‑1 (MQ=255)

                                                              tGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATGGAAAACaaaa                                                                          >  2:39891/1‑141 (MQ=255)

                                                              tGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                          <  1:48344/141‑1 (MQ=255)

                                                              tGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAAAAAACaaaa                                                                          <  2:17366/141‑1 (MQ=255)

                                                              tGGATTTATAAATATCCAGA‑AATTTTCTTTATAATGTTAGAACTATTACTTAAAAAGAGAAAGGTGTATACTACAACT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                          >  2:281437/1‑141 (MQ=255)

                                                                gTTTTCTAAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                        <  2:363355/141‑1 (MQ=255)

                                                                  tttCTCAATATACAGA‑AATTTTATTAATAATAGAAGCACAAATTCTTATGCTGTTGAATTACTATAAAACAAAT‑AAAAAATATACATCAAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGa                                                                      <  2:22209/141‑1 (MQ=255)

                                                                   ttCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGaa                                                                     <  1:203787/141‑1 (MQ=255)

                                                                         gtatCGAGA‑GAGTTTGTTTATAAGTGGAGCAGTGTTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATAGACATCTAAAGGTTAAAGTAATGAAAAATAGAATGAGAGAATATAGAAAACAAAAAGGAATTtcgc                                                               <  2:213733/140‑3 (MQ=255)

                                                                         atatCCAGA‑AAGTATAATAATAATAAAAGCAGTATTTCTTATGCTGTTTAATTACGAAACTACAAA‑GAAAAAATATACAACAAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTtctc                                                               <  2:338563/141‑1 (MQ=255)

                                                                          tatCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGACAACAAAAAGGAATTTCTCa                                                              >  1:224193/1‑141 (MQ=255)

                                                                          tatCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATAAAGAAAACAAAAAGGAATTTCTCa                                                              <  2:149987/141‑1 (MQ=255)

                                                                            tCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTAATATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                            <  1:388086/141‑1 (MQ=255)

                                                                             ccAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGa                                                           <  2:306573/141‑1 (MQ=255)

                                                                              cAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGaa                                                          >  2:378457/1‑141 (MQ=255)

                                                                               aGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGTATTACTCAAGaaa                                                         >  2:287604/1‑141 (MQ=255)

                                                                                 a‑aaTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAAAAGAATTAGAAAATATAGAAAAAAAAAAGGAATTTCTCAAGAAACt                                                       >  2:348072/1‑141 (MQ=255)

                                                                                        tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                 >  1:96921/1‑141 (MQ=255)

                                                                                        tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                 >  1:294646/1‑141 (MQ=255)

                                                                                        tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                 >  2:18160/1‑141 (MQ=255)

                                                                                             ataATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGa                                            <  1:202337/141‑1 (MQ=255)

                                                                                             ataATTGTAGCACTATTTCTTACGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAACCACCATTATCACAAGAAACTTTAAGTAAAGa                                            <  1:395506/141‑1 (MQ=255)

                                                                                               aaTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAGGAAACTTTAAGTAAAGAAt                                          <  1:219510/141‑1 (MQ=255)

                                                                                                aTTGTAGCCCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                         <  1:33929/141‑1 (MQ=255)

                                                                                                aTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                         <  1:261943/141‑1 (MQ=255)

                                                                                                  tGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaa                                       >  2:265121/1‑141 (MQ=255)

                                                                                                   gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                      >  2:341998/1‑141 (MQ=255)

                                                                                                   gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                      >  2:211928/1‑141 (MQ=255)

                                                                                                   gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                      <  1:247609/141‑1 (MQ=255)

                                                                                                   gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACATTAAGTAAAGAATTaaa                                      >  1:29472/1‑141 (MQ=255)

                                                                                                     aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATGAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAg                                    >  1:251176/1‑141 (MQ=255)

                                                                                                      gCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTGTAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTGAAGTAAAGAATTAATAgt                                   >  1:324677/1‑141 (MQ=255)

                                                                                                      gCACTATTTCTTATGCTGTTTAATTAATATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgt                                   <  1:241977/141‑1 (MQ=255)

                                                                                                       ctcTATTGCTTTTGCTGTTATATCATTAGACAACAAAT‑AAAAAATATACAGCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAGAgtg                                  <  2:77321/139‑1 (MQ=255)

                                                                                                       cACTATTTCTTATGCTGTTTAATTACTATACTACAAA‑AAAAAAATATACATCTAAAGGTTAAAATAAAAAAAAATAGAATTAGAGAAGATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtg                                  <  2:22225/141‑1 (MQ=255)

                                                                                                        aCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                 <  1:57580/141‑1 (MQ=255)

                                                                                                         cTATTTCTTATGCTGTTTAATTACTATACTACAAA‑AAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAATCTTTAAGTACAGAATTAAAAGTGTc                                <  1:199812/141‑1 (MQ=255)

                                                                                                         atATTTCTTATGCTGTTTAATTACTATACAACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                                <  2:310421/140‑1 (MQ=255)

                                                                                                           aTTTCGTATGTGGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTa                              <  2:188116/141‑1 (MQ=255)

                                                                                                                ttATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                         <  1:18160/141‑1 (MQ=255)

                                                                                                                ttATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                         >  1:4616/1‑141 (MQ=255)

                                                                                                                 tATGCTGTTTAATTACTATACTTCAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaaa                        >  2:36451/1‑141 (MQ=255)

                                                                                                                    tcTGTTTAATTACTATACTA‑AAAAAAAAAAATATACATCTAAAGGTTAAATAAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTa                     <  1:183754/140‑1 (MQ=255)

                                                                                                                       gTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTa                  >  1:119283/1‑141 (MQ=255)

                                                                                                                       gTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTa                  >  1:247130/1‑141 (MQ=255)

                                                                                                                        tttAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTaa                 >  2:224792/1‑141 (MQ=255)

                                                                                                                           aaTTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGAGCCTAGGCAAACCATTAAtac              >  2:13327/1‑139 (MQ=255)

                                                                                                                           aaTTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTAAAGAAACTTTAAGTAAAGAATTAAAAGTGTATAGGCAAACTAGTAATGc              >  1:149280/1‑141 (MQ=255)

                                                                                                                           aaTTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATAAAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGc              <  1:352173/141‑1 (MQ=255)

                                                                                                                            aTTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTACAAGTGTCTAGGCAAACTATTACTGCa             >  2:236611/1‑141 (MQ=255)

                                                                                                                             ttACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa            >  1:102726/1‑141 (MQ=255)

                                                                                                                             ttACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa            <  1:341705/141‑1 (MQ=255)

                                                                                                                              tactatactaCAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt           >  1:146941/1‑141 (MQ=255)

                                                                                                                              tactatacta‑AAAAAAAAAAAAATAAAGCTAAAGGTTAAATTAATGAAAAATAGAAAAAGAAAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt           <  1:277774/141‑1 (MQ=255)

                                                                                                                                ctatactaCAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAg         >  1:383126/1‑141 (MQ=255)

                                                                                                                                  atactaCAAAT‑AAAAAATATACATCTCAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaa       >  1:135061/1‑141 (MQ=255)

                                                                                                                                    actacAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaaaa     >  1:131493/1‑141 (MQ=255)

                                                                                                                                       acAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaa  >  1:80692/1‑141 (MQ=255)

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGA‑AATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAA  >  CP000731/25523‑25798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`