| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 97.6% | 130.6 / ‑3.4 | 41 | coding (45/1251 nt) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (17/23); minor base . (1/0); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAC > USA300TCH1516_ALE/2532162‑2532426 | gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa < 2:148460/141‑1 (MQ=255) tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CAt < 1:50075/141‑1 (MQ=255) tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CAttt < 1:139385/141‑1 (MQ=255) cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTcaca < 2:300457/141‑1 (MQ=255) aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACAt > 1:114055/1‑141 (MQ=255) ttttGATCAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTAAC‑TCAAAGTCATTTTTCACATTac > 2:38213/1‑141 (MQ=255) tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTacaca < 1:114918/141‑1 (MQ=255) aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAAt < 1:134442/141‑1 (MQ=255) aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAAt < 1:81139/141‑1 (MQ=255) aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTa > 2:386475/1‑141 (MQ=255) aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTa > 1:341037/1‑141 (MQ=255) aaaagTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTAt < 2:195966/141‑1 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTAt < 1:13080/141‑1 (MQ=255) aaaTAGCATTATTAATAAATTCAAAAATAATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTc < 1:277414/141‑1 (MQ=255) tAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCAtt < 1:2614/141‑1 (MQ=255) tAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCAtt > 1:119610/1‑141 (MQ=255) tattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATc < 2:131688/141‑1 (MQ=255) attaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCa < 1:49348/141‑1 (MQ=255) attaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCa < 1:184087/141‑1 (MQ=255) aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag < 1:192466/141‑1 (MQ=255) ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga < 1:240108/141‑1 (MQ=255) ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga > 1:181361/1‑141 (MQ=255) aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCTCATTACACACAATCAGCTATTCATTACAATCATAGAGtt > 2:6927/1‑141 (MQ=255) cAAAAATTATAGTTAAATTCAGAGAGGGATGGGATAAAATGGACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTGGAtt > 1:211264/1‑141 (MQ=255) ataGTTCAATTCAGAGCGGGATGGGATACAACGAACTTTGTAACGTTGACTTAGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACCTTCCACACAATAAGCTATTCATTCCAATCATAGAGTTGATTTAACAGGc > 2:108467/1‑141 (MQ=255) ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCCATGCATTACAATCATAGAGTTGATTTAAAAGGc < 1:192571/141‑1 (MQ=255) taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg > 2:107565/1‑141 (MQ=255) aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGa > 1:377248/1‑141 (MQ=255) gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGTATGAGTTACGTGAGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTCCAATCATAGAGATGAATTAAAAGTCGAt > 1:54860/1‑141 (MQ=255) tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc < 1:317129/141‑1 (MQ=255) cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGGGGTCGATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt > 1:77209/1‑141 (MQ=255) agagagGGATGTGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CAGTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt < 1:82333/141‑1 (MQ=255) agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt < 1:398476/141‑1 (MQ=255) gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTa < 1:350025/141‑1 (MQ=255) agGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAg > 1:105346/1‑141 (MQ=255) agaacaTGGACTTGGTAACGTTGACGTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCAGTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg < 2:59623/136‑1 (MQ=255) aaCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTGCACACAATCAGCTAGTCATTACAATCATAGAGATAATTAAAAAGGCGATGTGCATCTAGTAGGGGTTAAAGATGACAAt > 2:186080/1‑141 (MQ=255) aCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCCCTGATGGGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg > 1:91940/1‑141 (MQ=255) gtggACTTCGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt < 2:167999/138‑1 (MQ=255) cGGATGAGTTCAATGCGTTTACTACAAAG‑CATTTTTCAAATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt > 2:65874/1‑141 (MQ=255) aGTTCAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt > 1:255362/1‑141 (MQ=255) cAATGCGTTTACTACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc < 1:251078/141‑1 (MQ=255) | GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAG‑CATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAC > USA300TCH1516_ALE/2532162‑2532426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |