Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000731 81 N→G intergenic (–/‑151) – / → repA –/replication protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000731810NG98.8% 1702.1 / NA 496intergenic (–/‑151)–/repA–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (282/205);  minor base A (1/5);  total (285/211)
Fisher's exact test for biased strand distribution p-value = 8.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 497 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGAGGTTTTT  >  CP000731/1‑227
                                                                                                                                                 |                                                                                                                                                  
gtttatgatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAgaag                                                                                                                                                 >  2:1092636‑M1/66‑148 (MQ=255)
  ttatgatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAACACGTTTAGAAACt                                                                                                                                               <  1:1071442‑M1/86‑1 (MQ=255)
  ttatgatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAATCGTTTAGAAACt                                                                                                                                               >  1:960505‑M1/64‑149 (MQ=255)
     tgatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtt                                                                                                                                            >  2:294663‑M1/61‑149 (MQ=255)
     tgatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtt                                                                                                                                            >  1:715141‑M1/61‑149 (MQ=255)
     tgatatttatcaagggtttataaacccttgataaagctatattttccctttgataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtt                                                                                                                                            >  1:297037‑M1/61‑149 (MQ=255)
      gatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGttt                                                                                                                                           >  1:62757‑M1/60‑149 (MQ=255)
      gatatttatcaaggggttataaactcttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAAAATAACAAAAACCCCAACTACGGGTAATAGGCGGGTTTTGGTAATAAAACGTTTAGAAACTGttt                                                                                                                                           <  2:345541‑M1/90‑1 (MQ=255)
       agctttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTAttta                                                                                                                                          <  1:662311‑M1/91‑1 (MQ=255)
             atcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtata                                                                                                                                    <  1:751106‑M1/97‑1 (MQ=255)
             atcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtata                                                                                                                                    >  2:1284707‑M1/53‑149 (MQ=255)
             atcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAAAGTTTAGAAACTGTTTATTtata                                                                                                                                    <  2:524176‑M1/97‑1 (MQ=255)
               caagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCGCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                                  <  2:962918‑M1/99‑1 (MQ=255)
               caagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAAATGTTTATTtatata                                                                                                                                  <  1:36633‑M1/99‑1 (MQ=255)
                 agggtttacaaacccttgacaaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAt                                                                                                                                >  1:381926‑M1/49‑149 (MQ=255)
                     tttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAACAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTc                                                                                                                            >  1:1220433‑M1/45‑149 (MQ=255)
                          aaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAc                                                                                                                       <  1:1044233‑M1/110‑1 (MQ=255)
                           agcccttaataaagctaggtattcccttttataagtctATTTACAGTGCTACATTATTCTTTTTATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                                      <  2:1171147‑M1/111‑1 (MQ=255)
                           aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                                      >  1:581522‑M1/39‑149 (MQ=255)
                           aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                                      <  2:689408‑M1/111‑1 (MQ=255)
                            acccttgataaagctatattttcccttgtataagtttATTAAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAAAAACTGTTTATTTATATAATTTTAGAGACtt                                                                                                                     <  1:525853‑M1/112‑1 (MQ=255)
                               cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGta                                                                                                                  <  1:1345882‑M1/115‑1 (MQ=255)
                                 tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtatt                                                                                                                >  1:105738‑M1/33‑149 (MQ=255)
                                    taaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAACAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtata                                                                                                             >  1:596321‑M1/30‑149 (MQ=255)
                                      aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtatata                                                                                                           <  2:890410‑M1/122‑1 (MQ=255)
                                          tatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTACGATACTTGTATTATATAATGa                                                                                                       >  1:703593‑M1/24‑149 (MQ=255)
                                           ttattttcccttttacaagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTCCGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaa                                                                                                      >  1:644892‑M1/23‑149 (MQ=255)
                                                ttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAataata                                                                                                 >  1:190995‑M1/18‑149 (MQ=255)
                                                ttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAataata                                                                                                 >  1:1108399‑M1/18‑149 (MQ=255)
                                                ttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAataata                                                                                                 <  1:1009338‑M1/132‑1 (MQ=255)
                                                 tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGATTAatat                                                                                                <  2:709464‑M1/133‑1 (MQ=255)
                                                 tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAatat                                                                                                <  1:935697‑M1/133‑1 (MQ=255)
                                                    cttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCtt                                                                                             <  1:490018‑M1/136‑1 (MQ=255)
                                                           aagtttTTTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCaa                                                                                      >  1:569370‑M1/7‑149 (MQ=255)
                                                           aagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCaa                                                                                      >  1:176848‑M1/7‑149 (MQ=255)
                                                             gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGc                                                                                    >  2:98410‑M1/5‑149 (MQ=255)
                                                               ttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                                  <  1:770478‑M1/147‑1 (MQ=255)
                                                                tATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                                 <  1:1124052‑M1/148‑1 (MQ=255)
                                                                 aTTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCt                                                                                <  1:100122/149‑1 (MQ=255)
                                                                   ttAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaa                                                                              <  2:79482/149‑1 (MQ=255)
                                                                    tAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaaa                                                                             >  2:442494/1‑149 (MQ=255)
                                                                      aaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATc                                                                           >  1:1301559/1‑149 (MQ=255)
                                                                         ttatataATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAtt                                                                        <  2:173592/146‑1 (MQ=255)
                                                                             ataataAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGATTAGAAACTGTTTATTTATATAATTTTAGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTGAATCATtaaat                                                                    >  2:766905/1‑149 (MQ=255)
                                                                              taataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGCAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaata                                                                   >  2:268820/1‑149 (MQ=255)
                                                                                ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATTTTAGTGCAAGCACCTAAATCATtaaataaa                                                                 <  2:1005141/149‑1 (MQ=255)
                                                                                ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                                 <  1:1180202/149‑1 (MQ=255)
                                                                                ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                                 >  1:102882/1‑149 (MQ=255)
                                                                                ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                                 >  1:213630/1‑149 (MQ=255)
                                                                                ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                                 <  1:465293/149‑1 (MQ=255)
                                                                                ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                                 <  1:282043/149‑1 (MQ=255)
                                                                                 taaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATACAACGTTTAGAAACTGTTTATTTATATAATTTTAGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAGATCATTaaataaat                                                                >  2:870374/1‑149 (MQ=255)
                                                                                   aaCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATga                                                              >  2:430068/1‑148 (MQ=255)
                                                                                    aattAATATAACAAAAACCCCTACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAATTGTa                                                             <  2:939838/147‑1 (MQ=255)
                                                                                    aCTTAATATAACAAACACCCCAACTACTGGTAATAGTCGGGGTTTGGTACTTAAACGTTAAGAACCTGTTTATTTCTATCATGTTCGATACTTGTATTATATCATGAAAAATATCTTAGTGCAAGCACCTAAATACTTAAATAAATGTa                                                             >  2:505080/1‑149 (MQ=255)
                                                                                    aCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTa                                                             <  2:1100101/149‑1 (MQ=255)
                                                                                      ttAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaaa                                                           >  1:918294/1‑149 (MQ=255)
                                                                                      ttAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaaa                                                           >  1:837756/1‑149 (MQ=255)
                                                                                       tAATATAACAAAAACCCCAACTACAGGTAGTAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTATTGCAAGCACCTAAATCATTAAATAAATTTaaat                                                          >  1:687975/1‑148 (MQ=255)
                                                                                        aagataACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGc                                                         <  2:377101/146‑1 (MQ=255)
                                                                                        aaTATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGc                                                         <  1:475501/149‑1 (MQ=255)
                                                                                        aaTATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGc                                                         <  2:746797/149‑1 (MQ=255)
                                                                                         atatAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCt                                                        <  1:550660/149‑1 (MQ=255)
                                                                                          tataACAAAAACCCCAACTGCTTGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCCAGCACCTAAATCATTAAATAAATGTAAAGCta                                                       <  1:855728/149‑1 (MQ=255)
                                                                                           ataACAAAAACCCCAACGACTGGTAATAGTCGGGGTTTAGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaa                                                      <  2:629821/149‑1 (MQ=255)
                                                                                            taACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTACAAGCACCTAAAGCATTTAATAAATGTAAAGCtaaa                                                     >  1:448125/1‑149 (MQ=255)
                                                                                             aaCAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTTGTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                                    >  1:978760/1‑149 (MQ=255)
                                                                                              aCAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                                   >  1:127152/1‑149 (MQ=255)
                                                                                              aCAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTGATTTGTATAATTTTCGATACTTGTATTATATAATGCATAATATCTTAGTGCACGCAGTTAAATCATTAAATAAATTTAACGCtaaata                                                   >  1:31826/1‑149 (MQ=255)
                                                                                                 aaaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATaaaa                                                <  2:578873/149‑1 (MQ=255)
                                                                                                  aaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATaaaaa                                               >  2:457655/1‑149 (MQ=255)
                                                                                                    aCCCCAACTGCTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                             <  2:130561/149‑1 (MQ=255)
                                                                                                    aCCCAAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                             >  2:230006/1‑149 (MQ=255)
                                                                                                       ccAACTACTTGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGttt                                          <  1:1245534/149‑1 (MQ=255)
                                                                                                        cAACTATTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAGATAAAAACGtttt                                         <  2:158806/149‑1 (MQ=255)
                                                                                                         aaCTACTGGTAATAGTCGTGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTtta                                        <  2:1133828/149‑1 (MQ=255)
                                                                                                                 gTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACctct                                <  1:113945/149‑1 (MQ=255)
                                                                                                                  tAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCtt                               <  2:1077004/149‑1 (MQ=255)
                                                                                                                  tAATAGTCGGGGTTTCGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATACTATCTTCGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCtt                               >  2:1222567/1‑149 (MQ=255)
                                                                                                                    aTAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAc                             <  1:952251/149‑1 (MQ=255)
                                                                                                                      aGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCa                           >  2:133550/1‑149 (MQ=255)
                                                                                                                       gttggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaa                          <  2:666270/146‑1 (MQ=255)
                                                                                                                       gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaa                          <  2:1181134/149‑1 (MQ=255)
                                                                                                                       gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaa                          <  1:783984/149‑1 (MQ=255)
                                                                                                                       gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaa                          <  1:1090512/149‑1 (MQ=255)
                                                                                                                        tCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                         >  1:730031/1‑149 (MQ=255)
                                                                                                                        tCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                         >  1:241196/1‑149 (MQ=255)
                                                                                                                            ggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaa                     >  2:55838/1‑149 (MQ=255)
                                                                                                                            ggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaa                     >  1:420051/1‑149 (MQ=255)
                                                                                                                             gTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaaa                    <  1:268820/149‑1 (MQ=255)
                                                                                                                              tgtGGTAATAAAACGTTTAGATACTGTTTAGTTATAGATATTTCGTGACTTGTATTCTATAATGAATGATATCTTAGTGCATGCACCAAAATCCTTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTGCCAAACTatta                   <  2:83865/147‑4 (MQ=255)
                                                                                                                               ttGGTAATAAAACGTTTAGAAACCTTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATAATTAAATAAATGTAAAGCTAAATAAAAGCGTTTTATTACCTCTTACCAAACTAAAat                  <  2:1255027/149‑1 (MQ=255)
                                                                                                                                  gTAATAAAACGTTTAGCAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTATTAACAAACTAAGATATg               >  2:1033667/1‑149 (MQ=255)
                                                                                                                                   taataaACCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATgg              <  2:653533/149‑1 (MQ=255)
                                                                                                                                    aataaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGa             <  2:407157/149‑1 (MQ=255)
                                                                                                                                     ataaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGaa            >  2:195373/1‑149 (MQ=255)
                                                                                                                                        aaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAaga         <  1:87506/149‑1 (MQ=255)
                                                                                                                                         aaCGTATAGAAACCGTTTATTAATATAATTTTCGATACTTGTCTTATATAATGAATAATAACATAGTGCAAGCACCTAAATCATCAAATAAATGTAAAGATAAATTGAAAGCAATGATTACTTCTCAACAAACAAAAATATGGAAagat        >  2:538959/1‑148 (MQ=255)
                                                                                                                                               tAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGAGGttttt  >  1:1166472/1‑149 (MQ=255)
                                                                                                                                               tAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGAGGttttt  >  2:1193198/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGAGGTTTTT  >  CP000731/1‑227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑