BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	99.1%	357.0 / NA	105	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (41/63); total (42/63)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 107 total aligned reads displayed.

TTTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTAT  >  USA300TCH1516_ALE/1646043‑1646333

tttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGcctcct                                                                                                                                                <  2:704831/149‑1 (MQ=255)

tttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGcctcct                                                                                                                                                <  2:756968/149‑1 (MQ=255)

     ataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGGCTCCTTTTac                                                                                                                                           >  1:352902/1‑149 (MQ=255)

     ataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCATTTAATTCTTTTTCATTAATGCCTCCttttgc                                                                                                                                           >  1:586433/1‑147 (MQ=255)

         ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                                       >  2:799269/1‑149 (MQ=255)

          tttATCACTTGCAATACCAGTTGGTAATGCACCTCTATGTTCATATGCTAATATCCTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTATCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAg                                                                                                                                      <  1:178804/149‑1 (MQ=255)

          tttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGACGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTCGCATTTTGCTTTCTAATATTGCCTTTTATTCTTTTTCAGTCATGCCTCCTTTTACAACAg                                                                                                                                      <  1:601203/149‑1 (MQ=255)

            tatatcTTGCACCACCATGTGTTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTCTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                    <  2:921467/144‑1 (MQ=255)

            tATCACTTGCCACCCCCTGTGGTCATGCCACCATCTGACCCAATCCTGCAAACTTATCCAATGAGTATCCATCTACGCCTAAGT‑‑CTAGCACTGTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                    <  2:116533/149‑1 (MQ=255)

            tATCACTTGCAACACCATGTTGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                    <  1:169503/149‑1 (MQ=255)

            tATCACTTGCAACACCATGTCGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                    <  1:835825/149‑1 (MQ=255)

             aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTa                                                                                                                                   <  2:292225/149‑1 (MQ=255)

              tCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                                  <  1:820779/149‑1 (MQ=255)

              tCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                                  <  1:820529/149‑1 (MQ=255)

              tCACTTGCAACACCATGTAGTAATGCACCTCTATGACCAGATGCAACAAACACCTTTTGTGATGGTCCATCTGCACCTACTT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCCTGCCTCCTTTTACAACAGTTaa                                                                                                                                  <  1:701907/149‑1 (MQ=255)

              tCACCTGCAACACCATGTTGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                                  <  1:1067904/149‑1 (MQ=255)

               ctcTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                                 <  2:174107/147‑1 (MQ=255)

               cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                                 <  2:1330822/149‑1 (MQ=255)

               cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                                 <  2:492058/149‑1 (MQ=255)

                aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                                >  1:1008312/1‑149 (MQ=255)

                 cTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAat                                                                                                                               <  1:828089/149‑1 (MQ=255)

                 cTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAat                                                                                                                               <  1:1136653/149‑1 (MQ=255)

                   tGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAaaat                                                                                                                             >  1:533781/1‑149 (MQ=255)

                      aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTACTATTGCCTTTGATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                                                                                          >  2:281796/1‑149 (MQ=255)

                       acccaaTGTGGTAATGCACCTCTATGAACAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                                         <  2:464138/144‑1 (MQ=255)

                       acacCATGTGGTAATGCACCTCTATGACCAGACGATACAATCCTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                                         <  2:16119/149‑1 (MQ=255)

                        cacCATGTTGTAATGCACCTCTATGACCAGATGCTACCCTCGAATCGACCGATCTGATAGCTGCGCCGAATT‑‑CTAGCATTTTTCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                                        <  2:945978/149‑1 (MQ=255)

                        cacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                                        >  1:400961/1‑149 (MQ=255)

                        cacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGCAGCGCCTGCTCCTAATTCACCAGCAGCT‑‑CTTTCTAATATTACCGTGAATGCTTTTTTAGTCATTCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                                        >  1:316253/1‑149 (MQ=255)

                         acCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCAGGCGCTTATT‑‑CTAGCCTTTTGCTTTCTAATATTGCCTTTAATACTTGTTCAGTCATACCTCCTTTTACAACAGTTAAACTATATTCat                                                                                                                       >  1:643307/1‑149 (MQ=255)

                              gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                                                                                  <  1:110579/149‑1 (MQ=255)

                              gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                                                                                  <  1:110468/149‑1 (MQ=255)

                                ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTGTCAGTCATGCCTCCTTTTACAACGGTTAAAATATATTCATATGtttt                                                                                                                >  2:66459/1‑148 (MQ=255)

                                     tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                           <  2:78122/149‑1 (MQ=255)

                                       ccccTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaaca                                                                                                         <  1:1180512/147‑1 (MQ=255)

                                         ccTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAat                                                                                                       <  2:1103471/149‑1 (MQ=255)

                                             tATGACCAGATGCTACAATCGTATCGAGTGATGGTCCATCTGCTCCTAATA‑‑CTAGCATTTTGCTTTCTAATAATGACTTTCACTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTa                                                                                                   >  1:1003076/1‑149 (MQ=255)

                                             tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGTCCTTCGGCCCCGGATATTGGCGTAAATTCTTTTTCAGTCATGCCTCGTTTTACAACGGTTAAAATATATTCATATGTTTCATGAACAATATTa                                                                                                   >  1:315923/1‑149 (MQ=255)

                                             tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTa                                                                                                   >  2:958163/1‑149 (MQ=255)

                                             tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTa                                                                                                   >  1:1343665/1‑149 (MQ=255)

                                                 cccAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgatg                                                                                               >  1:861743/2‑149 (MQ=255)

                                                 aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTGTTTTTCAGTCATGCCTCCTTTTACAACAGTTCAAATATATTACTTTTTTTCATCAACAATATTAgctg                                                                                               >  1:1251579/1‑149 (MQ=255)

                                                 aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAAAAATATTAgctg                                                                                               >  1:132805/1‑149 (MQ=255)

                                                     gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttt                                                                                           >  2:806405/1‑149 (MQ=255)

                                                      aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                          <  2:1025720/149‑1 (MQ=255)

                                                      aTGCTACAATCGTACCGAATGATGGTCCATCTGCTCCTAATG‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                          <  2:708141/149‑1 (MQ=255)

                                                       tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttttt                                                                                         <  2:162985/149‑1 (MQ=255)

                                                         ttACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGa                                                                                       <  2:135134/148‑1 (MQ=255)

                                                         cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGa                                                                                       <  1:531965/149‑1 (MQ=255)

                                                         cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGa                                                                                       <  1:884694/149‑1 (MQ=255)

                                                           aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                                     <  2:131813/149‑1 (MQ=255)

                                                              aTCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACATTATTAGCTGCTTTTTGAATTaa                                                                                  <  2:913585/149‑1 (MQ=255)

                                                              aTCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                                  <  2:489750/149‑1 (MQ=255)

                                                                       aaTGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                         <  1:578182/149‑1 (MQ=255)

                                                                        atgatgGTCCATCTGGTCCTAATT‑‑CTAGAATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTATAATATATTCATATGTTTCATCAACAATATTAGCTACTTTTTGAATTAAAGCAATTTCg                                                                        >  1:846891/1‑149 (MQ=255)

                                                                        atgatgGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTct                                                                        >  2:1158439/1‑148 (MQ=255)

                                                                        atgatgGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg                                                                        >  1:1298425/1‑149 (MQ=255)

                                                                        atgatgGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTACCTCAACAATATTAGCTGCTCTCTGAATTAAAGCAATTTCg                                                                        <  2:888103/149‑1 (MQ=255)

                                                                        atgatgGTCCATCTGCTCCCAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg                                                                        <  2:653988/149‑1 (MQ=255)

                                                                         tgatgGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGt                                                                       >  1:1037065/1‑149 (MQ=255)

                                                                               tCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                                 >  1:1078184/1‑149 (MQ=255)

                                                                               tCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                                 >  1:24644/1‑149 (MQ=255)

                                                                               tCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAAAAATATTAACTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                                 >  1:578871/1‑149 (MQ=255)

                                                                                ccATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTccgactc                                                                >  2:309101/1‑143 (MQ=255)

                                                                                  aTCTGCGCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                              <  2:249696/149‑1 (MQ=255)

                                                                                   tCTGCTCCTAATT‑‑CTAGCTGTTTGCTTTCTAATATTGCCTTTAATTCCTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                             <  1:549719/149‑1 (MQ=255)

                                                                                         ccTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                                       <  1:704663/149‑1 (MQ=255)

                                                                                         ccTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                                       <  1:1345025/149‑1 (MQ=255)

                                                                                         ccTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                                       <  1:806405/149‑1 (MQ=255)

                                                                                         ccTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                                       <  1:1291864/149‑1 (MQ=255)

                                                                                          cTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                      <  2:619922/149‑1 (MQ=255)

                                                                                             aTT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTACCCTATTTTATTTATGGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                                   >  1:373698/1‑149 (MQ=255)

                                                                                             aTT‑‑CGAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGTCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCCATTTCGTCAGCATCTTTGACGTCTCTa                                                   <  2:916147/149‑1 (MQ=255)

                                                                                                  cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                                <  2:1033913/149‑1 (MQ=255)

                                                                                                  cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                                <  2:1096862/149‑1 (MQ=255)

                                                                                                     gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                             <  1:1202015/149‑1 (MQ=255)

                                                                                                      cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                            <  2:561765/149‑1 (MQ=255)

                                                                                                      cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                            >  1:1251561/1‑149 (MQ=255)

                                                                                                      cATTTTGCTTTCTAATATTGCCTTTAATTCTTTATCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCGTCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                            <  2:748264/149‑1 (MQ=255)

                                                                                                          ttGCTTTCTAAGATGGCCTTTAATTCTTTTTCAGGCATGCCTCCTTTTACACTAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTAc                                        <  1:309101/149‑1 (MQ=255)

                                                                                                            gCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAAAATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAg                                      >  1:827974/1‑149 (MQ=255)

                                                                                                                tCTAAGATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                                  >  2:164913/1‑149 (MQ=255)

                                                                                                                  tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                                <  1:281796/149‑1 (MQ=255)

                                                                                                                     tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTATTTTTATCTACAGTTATAGaaa                             >  2:342678/1‑149 (MQ=255)

                                                                                                                     tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                             >  1:750924/1‑149 (MQ=255)

                                                                                                                         gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                         <  2:1221868/149‑1 (MQ=255)

                                                                                                                             ttAATTCTTTTTCAGTCATGCCTGCTTTTACAAGAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt                     >  1:671436/1‑149 (MQ=255)

                                                                                                                              tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTAATTAAAGCAATTTCGTCAGCATCTTTGACGTTTCTAATTTTATCTACAGTATTAGAAATGCttatta                    >  2:424353/1‑149 (MQ=255)

                                                                                                                              tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCGACGTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCGCTAATTTTATCTACCGTATTAGAAATGCtttttc                    >  2:916057/1‑148 (MQ=255)

                                                                                                                                 ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg                 <  1:534827/149‑1 (MQ=255)

                                                                                                                                 ttCTTTTTCAGTCATGCCTCCCTCTTCAACCGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACCGTATTAGTAATGCTTATTAATg                 <  1:855841/149‑1 (MQ=255)

                                                                                                                                     ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTTTTTTTTTTAATTTCGTCAGCATCTTTGCCGTCTCTCATTTTATCTACAGTATTAGAAATGCTTATTATTGatat             >  2:1072376/1‑149 (MQ=255)

                                                                                                                                     ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat             >  1:788482/1‑149 (MQ=255)

                                                                                                                                       ttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTCTTAGAAATGCTTATTACTGATATAc           >  2:123444/1‑149 (MQ=255)

                                                                                                                                         cAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAcag         >  2:1060565/1‑147 (MQ=255)

                                                                                                                                          aGTCATTCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCTGCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc        <  2:179105/149‑1 (MQ=255)

                                                                                                                                              tcgCCTCCCTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGCCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtttt    <  1:243725/147‑1 (MQ=255)

                                                                                                                                              aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtttt    <  1:580140/149‑1 (MQ=255)

                                                                                                                                              aTGCCTCCTGTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtttt    <  1:1245332/149‑1 (MQ=255)

                                                                                                                                                gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTtttat  <  2:28331/149‑1 (MQ=255)

TTTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATT‑‑CTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTAT  >  USA300TCH1516_ALE/1646043‑1646333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`