BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,473	C→T	A67T (GCT→ACT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,473	0	C	T	100.0%	403.6 / NA	128	A67T (GCT→ACT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (45/83); total (45/83)

Only 100 of 128 total aligned reads displayed.

TAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTA  >  USA300TCH1516_ALE/1744327‑1744618

tAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTAACGAGGTAATTTCCCTTCTTTTCTACCAGGTGCAAAGCCAATCCCCATTGAATAAGTTa                                                                                                                                                 <  2:841445/149‑1 (MQ=255)

  acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAGCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGATGCAAAGCCAATCCACATTGAATAAGTTACa                                                                                                                                               >  1:709071/1‑149 (MQ=255)

  acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGTTAct                                                                                                                                               >  1:1040218/1‑148 (MQ=255)

    ccccGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg                                                                                                                                             <  2:883881/146‑1 (MQ=255)

    acacGTTGACGTGGGTTAATTGCATCTTTGGGCATTGTTAAAACATTTGTGCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAGTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg                                                                                                                                             <  2:924703/149‑1 (MQ=255)

    acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg                                                                                                                                             <  2:264611/149‑1 (MQ=255)

    acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg                                                                                                                                             <  2:901895/149‑1 (MQ=255)

           taCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGCACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCa                                                                                                                                      <  2:368390/148‑1 (MQ=255)

           gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTTTAGGTCATACTCATAACGCATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCa                                                                                                                                      <  2:336028/149‑1 (MQ=255)

           gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACTGCCa                                                                                                                                      <  2:1304791/149‑1 (MQ=255)

           gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCa                                                                                                                                      <  1:383748/149‑1 (MQ=255)

           caCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCa                                                                                                                                      <  2:657801/148‑1 (MQ=255)

            aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa                                                                                                                                     >  2:1170279/1‑149 (MQ=255)

              cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATg                                                                                                                                   <  1:417070/149‑1 (MQ=255)

               tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCGCATTGAATAAGTTACAGGACAGCCAATGa                                                                                                                                  <  1:693126/149‑1 (MQ=255)

                ggTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGAt                                                                                                                                 <  2:805670/149‑1 (MQ=255)

                  tttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTACCTTCCCTTCTTTTCTAACAGGTGCATAGCCAATCACCATTTCATCAGTTACAGAACAGCCACTGATaa                                                                                                                               >  1:312367/1‑149 (MQ=255)

                  tttAATTGCATCTTTGTGCATTGCTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTCCAGGACAGCCAATGATaa                                                                                                                               >  1:996128/1‑149 (MQ=255)

                        tGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc                                                                                                                         >  1:83310/1‑149 (MQ=255)

                          cctCTTTGTGCACTGTTAAAACATTTGTACCATATACTAGGTCATACTACTAACGACTGACTTGGCGCGGTCAGTTCACTTCTTTTCTAACAGGCGCAACGCCAATCCCCCTTGAATAAGTTACAGGACAGCCAATAATAAACCCAcgc                                                                                                                       >  2:26092/3‑149 (MQ=255)

                           aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAGGCCAcgcg                                                                                                                      >  2:833534/1‑149 (MQ=255)

                                tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACATCCAATGATAAAGCCACGCGCTTCa                                                                                                                 <  2:618735/149‑1 (MQ=255)

                                tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCa                                                                                                                 <  2:770201/149‑1 (MQ=255)

                                 ttgCATTGTTAAAACATTTCTACCATATTCGAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                                                                <  1:764544/148‑1 (MQ=255)

                                 gtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                                                                <  1:822608/149‑1 (MQ=255)

                                   gCATTGTTAAAACATTTGTACCATATTCTAGCTCATCCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt                                                                                                              <  1:565418/149‑1 (MQ=255)

                                          tAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                                                       <  1:1178445/149‑1 (MQ=255)

                                             aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa                                                                                                    <  1:30431/149‑1 (MQ=255)

                                              aCATTTGTACCATTTTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCAGCGCGCTTCAGGTCCTACAACGat                                                                                                   <  1:669704/149‑1 (MQ=255)

                                              aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTTTACCAGGTGCAAAGCCAATCCCCCTTGAATCAGTTACAGGACAACCAATGATCAAGCCCCGCGCTTCAGGTCCTACAACGat                                                                                                   >  2:546134/1‑149 (MQ=255)

                                              aCATTCGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat                                                                                                   <  1:934513/149‑1 (MQ=255)

                                                   tGTACCATATTCTAGGTCATACTCATAACGAATGACTTCCCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCaa                                                                                              >  2:512007/1‑149 (MQ=255)

                                                      cccATATTCTAGGTCATACTCATACCGAATGACTTCACGATGCAAGTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTCACAGGACAGCCACTGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt                                                                                           <  2:1068525/148‑1 (MQ=255)

                                                      aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCCCGAGCGCAAGGGCCTATAACGATATCAACAt                                                                                           >  2:663448/1‑149 (MQ=255)

                                                        cATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACNGCCAATGATAAAACCACGCGCTTCAGGTCNTNCAACGATATCAACAtct                                                                                         >  1:1144247/1‑149 (MQ=255)

                                                        cATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                                         >  1:1052030/1‑149 (MQ=255)

                                                         atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                                                        >  1:149517/1‑149 (MQ=255)

                                                            ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                                     <  2:562165/149‑1 (MQ=255)

                                                              cTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAGGCGCTTCAGGTCCTACAACGTTATCAACATATCTGTCt                                                                                   >  2:767598/1‑149 (MQ=255)

                                                                aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                                                 <  1:361986/149‑1 (MQ=255)

                                                                  gTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCACAATCTCTGCCTTTTg                                                                               >  1:71178/1‑149 (MQ=255)

                                                                  gTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                               >  1:572147/1‑149 (MQ=255)

                                                                  gTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                               >  1:572445/1‑149 (MQ=255)

                                                                  gTCATACCCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAGAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                               <  2:629167/149‑1 (MQ=255)

                                                                   tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCGTACAACGATATCAACATCTCTGTCTTTTGc                                                                              >  2:842285/1‑149 (MQ=255)

                                                                   tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc                                                                              >  1:628901/1‑149 (MQ=255)

                                                                   tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc                                                                              <  1:152777/149‑1 (MQ=255)

                                                                   tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc                                                                              <  1:1019245/149‑1 (MQ=255)

                                                                   tCATACTCACAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAACGATACAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc                                                                              <  2:552322/149‑1 (MQ=255)

                                                                    cacaCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCg                                                                             <  2:388226/146‑1 (MQ=255)

                                                                    cATACTCTTAACGAATCACTTCCCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCg                                                                             <  1:272157/149‑1 (MQ=255)

                                                                     gttcTCATAGCGAGTGTCTTCACGAGGTATTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCTCGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGt                                                                            <  2:190461/146‑1 (MQ=255)

                                                                     aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGt                                                                            <  2:577157/149‑1 (MQ=255)

                                                                      tACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTa                                                                           <  2:141234/149‑1 (MQ=255)

                                                                       aCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCATCATCTCTGTCTTTTGCGTAt                                                                          <  1:299705/149‑1 (MQ=255)

                                                                          cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACACCGATATCAACATCTCTGTCTTTTGCGTATTCt                                                                       <  1:981500/149‑1 (MQ=255)

                                                                          cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                                       <  1:1080143/149‑1 (MQ=255)

                                                                          cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCACCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                                       >  2:951091/1‑149 (MQ=255)

                                                                           aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAATCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTa                                                                      >  2:418365/1‑149 (MQ=255)

                                                                             aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                                                                    <  2:460719/149‑1 (MQ=255)

                                                                             aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                                                                    <  1:1261810/149‑1 (MQ=255)

                                                                             aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                                                                    <  2:884090/149‑1 (MQ=255)

                                                                               cGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCCGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAt                                                                  >  2:226089/1‑149 (MQ=255)

                                                                                     aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATc                                                            >  1:642281/1‑149 (MQ=255)

                                                                                     aCTTCACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATc                                                            >  2:1262630/1‑149 (MQ=255)

                                                                                       ttCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                                                          >  2:787831/1‑149 (MQ=255)

                                                                                       ttCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                                                          >  2:691724/1‑149 (MQ=255)

                                                                                        tCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCACTGATAAAGCCACGCGCTTCAGGTCCTACAACGACATCAACATTTCTGTCTTTTGCGTATTCTAACATTTTCTCTGt                                                         >  1:513283/1‑149 (MQ=255)

                                                                                        tCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                                                         <  1:995717/149‑1 (MQ=255)

                                                                                        acACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCTCCATTGAATAAGTTACAGGACCGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                                                         <  1:942808/148‑1 (MQ=255)

                                                                                         cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGGAAAGCCAATCCCCATTGAATACGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                                                        <  2:738571/149‑1 (MQ=255)

                                                                                         cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                                                        <  2:827723/149‑1 (MQ=255)

                                                                                                    ttCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata                                             >  2:403418/1‑149 (MQ=255)

                                                                                                              ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACTGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTCCATAGCCATATGCTTCACCa                                   <  1:950487/149‑1 (MQ=255)

                                                                                                                   aCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATc                              <  2:1190243/149‑1 (MQ=255)

                                                                                                                   aCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAGCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATc                              <  2:820752/149‑1 (MQ=255)

                                                                                                                     aGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                            <  2:572147/149‑1 (MQ=255)

                                                                                                                     aGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                            <  2:71178/149‑1 (MQ=255)

                                                                                                                      ggTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt                           >  2:1309241/1‑149 (MQ=255)

                                                                                                                         gCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAt                        <  2:111252/149‑1 (MQ=255)

                                                                                                                         gCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATACCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAt                        <  2:1078260/149‑1 (MQ=255)

                                                                                                                           aaaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACACCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATATCCATATGCTTCACCATTATCCATAtttt                      >  1:448858/1‑148 (MQ=255)

                                                                                                                            aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgt                     <  1:663448/149‑1 (MQ=255)

                                                                                                                             aGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAATGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgta                    <  2:729845/149‑1 (MQ=255)

                                                                                                                             aGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATACGCTTCACCATTATCCATAATTgta                    <  1:796770/149‑1 (MQ=255)

                                                                                                                             aGCCAATCCCCATTGAATAAGTTACAGGACAGACAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTATACAATTTTATCTGTTGCATAGCAATATGCTTCACCATTATCAATAATTgta                    >  1:1016218/1‑149 (MQ=255)

                                                                                                                              gCCAATCCCCATTTAATAAGTTACAGGACAGCCAATGAGAAAGCCACGCGATTCACGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTACTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtag                   <  2:877889/149‑1 (MQ=255)

                                                                                                                              gCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTAAGTAGCTATAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtag                   <  1:975739/149‑1 (MQ=255)

                                                                                                                                  aTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAat               >  2:527170/1‑149 (MQ=255)

                                                                                                                                   tCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAata              <  2:83310/149‑1 (MQ=255)

                                                                                                                                    ccccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATAGGCTTCCCCCTTATCCATAATTGTAGTAataa             >  2:53062/1‑148 (MQ=255)

                                                                                                                                      ccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATcc           >  2:1127889/1‑149 (MQ=255)

                                                                                                                                       cATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCt          <  2:905229/149‑1 (MQ=255)

                                                                                                                                       cATTGAATAAGTTACAGGACAACCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCt          <  2:866205/149‑1 (MQ=255)

                                                                                                                                        aTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCtt         >  2:1239694/1‑149 (MQ=255)

                                                                                                                                          tGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCTTATGCTTCACCATTATCCATAATTGTTGTAATATCCTTGa       >  1:392138/1‑149 (MQ=255)

                                                                                                                                          tGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTCGTAATATCCTTGa       >  1:1184294/1‑149 (MQ=255)

                                                                                                                                            aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGaaa     <  1:343581/149‑1 (MQ=255)

                                                                                                                                            aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCACGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGaaa     <  2:836765/149‑1 (MQ=255)

                                                                                                                                               aaGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTa  <  2:349954/149‑1 (MQ=255)

TAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTA  >  USA300TCH1516_ALE/1744327‑1744618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`