BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	26,015	C→G	A117G (GCG→GGG)	walK →	Sensor protein kinase WalK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	26,015	0	C	G	100.0%	467.0 / NA	131	A117G (GCG→GGG)	walK	Sensor protein kinase WalK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (66/65); total (66/65)

Only 100 of 131 total aligned reads displayed.

ATGCACAAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGCGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTAATATTT  >  USA300TCH1516_ALE/25877‑26158

aTGCACAAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGCCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGt                                                                                                                                       <  1:1018123/149‑1 (MQ=255)

   cacaAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCt                                                                                                                                    >  1:837432/1‑149 (MQ=255)

   cacaAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCt                                                                                                                                    >  1:744333/1‑149 (MQ=255)

   cacaAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAAGGATAGTTCt                                                                                                                                    >  1:170933/1‑149 (MQ=255)

   cacaAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAAAGATAGTTCt                                                                                                                                    >  1:195791/1‑149 (MQ=255)

         aaGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCaa                                                                                                                              >  2:907292/1‑149 (MQ=255)

         aaGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCaa                                                                                                                              >  2:656517/1‑149 (MQ=255)

         aaGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAAACGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTTTCCaa                                                                                                                              >  2:1257779/1‑149 (MQ=255)

            atatTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCaaaaa                                                                                                                           >  1:281659/1‑149 (MQ=255)

             tatTCAAAATTTATTAAGTGAGGATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAg                                                                                                                          <  2:408732/149‑1 (MQ=255)

              atTCAAAATTTATTAAGTGGGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATGGTTCTGTCCATAAAGc                                                                                                                         <  2:621818/149‑1 (MQ=255)

              atTCAAAATTTATTAAGTGAGTAATCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATCATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGc                                                                                                                         <  2:1079890/149‑1 (MQ=255)

              atTCAAAATTTATCAAGGGAGTATGCTAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCGAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGc                                                                                                                         <  2:106182/149‑1 (MQ=255)

                tCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTAACCAAAAAGCAc                                                                                                                       >  2:925007/1‑149 (MQ=255)

                   aaaTTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTAt                                                                                                                    >  2:84467/1‑149 (MQ=255)

                    aaTTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCGCTATc                                                                                                                   >  2:975000/1‑149 (MQ=255)

                      tttATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCAc                                                                                                                 >  2:1040093/1‑149 (MQ=255)

                        tattaAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTa                                                                                                               <  1:1235728/149‑1 (MQ=255)

                            aaGAGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGCTAAAGAACAATTTAATACTGCAACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGaa                                                                                                           >  2:614534/1‑148 (MQ=255)

                             aGTGAGTATGCCAACCGTCAAGAAATTGGCGAAGTTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACa                                                                                                          >  2:289116/1‑149 (MQ=255)

                              gTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATAAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGCCaa                                                                                                         >  1:1273657/1‑149 (MQ=255)

                               ttaGTATGCCAACCGACAAGAAATTGGAGAAATTCGTTTTATAGATAAAGCCGAAATTACGATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAAt                                                                                                        <  2:1004853/147‑1 (MQ=255)

                               tGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAAt                                                                                                        <  2:387415/149‑1 (MQ=255)

                               tGAGTATGCCAACCGTAAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAAt                                                                                                        <  2:811780/149‑1 (MQ=255)

                                 aGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCa                                                                                                      >  1:1177531/1‑149 (MQ=255)

                                 aGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCa                                                                                                      >  2:100158/1‑149 (MQ=255)

                                 aGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCa                                                                                                      >  1:103055/1‑149 (MQ=255)

                                       ccAACCGTCAAGAAGTGGGAGAAATTGGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAACCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGAt                                                                                                <  1:394374/149‑1 (MQ=255)

                                       ccAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGAt                                                                                                <  1:567369/149‑1 (MQ=255)

                                                 aGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAAttt                                                                                      <  1:191691/149‑1 (MQ=255)

                                                   aaaTTGGAGAACTTCGTTTTATAGATAAAGACCAAATTATTATTGCGAAGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTa                                                                                    <  1:297396/149‑1 (MQ=255)

                                                   aaaTTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTa                                                                                    <  2:100818/149‑1 (MQ=255)

                                                   aaaGTGGAGAGATTCGTTTTATCGATGAACACCAAATTATTATGGCGACGACGAAGCAGTCTAACCGTAGTCTAATCACTCAAACAGGGAATGATAGTTCTGTCCAAAAAGCGCTATCACTAGGACAATCAAGCGATCATTTAATTTTa                                                                                    <  2:761323/149‑1 (MQ=255)

                                                     aTTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTaaa                                                                                  <  1:1007844/149‑1 (MQ=255)

                                                     aTTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAATCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTaaa                                                                                  <  1:167176/149‑1 (MQ=255)

                                                          agaAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGAtt                                                                             >  2:457351/1‑149 (MQ=255)

                                                                 cGTTATATAGATAAAGACCAAATTAATATTGCGACGACGAAGCAGTCTAACCGTAGACAAAACAAAAAAAAAGGGAATGATAGTTCTGTCCAAAGAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCgg                                                                      <  2:855088/149‑1 (MQ=255)

                                                                  gTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCggt                                                                     <  1:1040093/149‑1 (MQ=255)

                                                                    tttATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAACAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTACGGCggtgg                                                                   >  1:1152887/1‑149 (MQ=255)

                                                                    tttATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCggtgg                                                                   >  1:111358/1‑149 (MQ=255)

                                                                    tttATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCggtgg                                                                   >  1:145916/1‑149 (MQ=255)

                                                                    tttATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGGTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCggtgg                                                                   >  1:238420/1‑149 (MQ=255)

                                                                           aTACAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGAc                                                            <  1:368828/149‑1 (MQ=255)

                                                                              aaGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCgg                                                         >  1:175432/1‑148 (MQ=255)

                                                                               aGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCgtg                                                        >  1:1323724/1‑149 (MQ=255)

                                                                                gACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCgtgt                                                       >  1:1323826/1‑149 (MQ=255)

                                                                                gACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAAACAAAAGATAATTTAATACTAAGAGAGTAGGGCGGTGGTAAGGACCgggt                                                       >  1:509034/1‑149 (MQ=255)

                                                                                 aCCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTc                                                      >  1:146188/1‑149 (MQ=255)

                                                                                  ccAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACGGTGTCt                                                     >  1:22162/1‑149 (MQ=255)

                                                                                    aaaTTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTgg                                                   <  1:295944/149‑1 (MQ=255)

                                                                                     cattattactGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCCCTATCACTAGGACAATCGAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTggg                                                  <  2:1101740/148‑1 (MQ=255)

                                                                                     aaTTATTATAGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTggg                                                  <  2:1041652/149‑1 (MQ=255)

                                                                                     aaTTATTAGTGCGACGTCGAAGCCGTCTATCCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTggg                                                  <  2:1165434/149‑1 (MQ=255)

                                                                                     aaTCATTATGGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTggg                                                  <  1:109590/149‑1 (MQ=255)

                                                                                      attattattGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGt                                                 <  1:183497/149‑1 (MQ=255)

                                                                                      attattattGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGt                                                 <  1:504158/149‑1 (MQ=255)

                                                                                      attattagtGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGt                                                 <  2:281659/149‑1 (MQ=255)

                                                                                       ttattattGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGta                                                <  2:1244654/149‑1 (MQ=255)

                                                                                        aattattGGGACGACGAAGCAGTCTAACCGTAGTAAAAACAATAGAACCGGGCATGACAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGtat                                               <  2:282596/148‑1 (MQ=255)

                                                                                         attattGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGtata                                              <  1:132602/149‑1 (MQ=255)

                                                                                          ttattGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTAGGGCGGTGGTAAGGACCGGGTCTGGGtaaat                                             >  1:518762/1‑149 (MQ=255)

                                                                                                cgactacgaAGCAGTCTAACCGTAGGCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATAATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATc                                       >  1:666715/1‑149 (MQ=255)

                                                                                                cgacgacgaAGCATTCTAACCGTAGTCTAATCTATGAAATAGGGAATGGTAGTTCTGTCCAAAAAGCACTATCAGTAGGACAATCAAACGATAATTTAATGTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAAGATc                                       >  1:989638/1‑149 (MQ=255)

                                                                                                cgacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATc                                       >  1:935318/1‑149 (MQ=255)

                                                                                                cgacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATc                                       >  2:1028631/1‑149 (MQ=255)

                                                                                                cgacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATc                                       <  1:662178/149‑1 (MQ=255)

                                                                                                cgacgacgaAGCAGTCAAACCGGAGTCTAATCAATCAAACAGGGAATGATAGTTATGACCATAAAGCACTATCACTAGGACAATCAATCGATCATTTAATTTTAAAAGATTATGCCGGTGGTAAGGACCGTGTCCGGGTAGATAATATc                                       >  2:284107/1‑149 (MQ=255)

                                                                                                 gacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAATAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATcc                                      >  2:753563/1‑149 (MQ=255)

                                                                                                 gacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATcc                                      >  2:771389/1‑149 (MQ=255)

                                                                                                 gacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATcc                                      >  1:739331/1‑149 (MQ=255)

                                                                                                 gacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGGATATAATATcc                                      >  1:1077997/1‑149 (MQ=255)

                                                                                                 gacgacgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATAATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATcc                                      >  1:94865/1‑149 (MQ=255)

                                                                                                  ccgacgaGGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAACGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATccc                                     <  2:816897/148‑1 (MQ=255)

                                                                                                     acgaAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGt                                  <  2:190315/149‑1 (MQ=255)

                                                                                                             gTCTAACCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGACTATAATATCCCAGTTAAAGTca                          >  2:325262/1‑148 (MQ=255)

                                                                                                             atcgaaCCGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCg                          <  1:787888/145‑1 (MQ=255)

                                                                                                             atCTAACCGTAGTCTAATCGATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCg                          <  1:1169888/148‑1 (MQ=255)

                                                                                                                   ccGTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATaaaa                    <  2:247552/149‑1 (MQ=255)

                                                                                                                     gTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATaaaagg                  >  2:1231021/1‑147 (MQ=255)

                                                                                                                     gTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAg                  >  2:151511/1‑149 (MQ=255)

                                                                                                                     gTAGTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAg                  >  1:326896/1‑149 (MQ=255)

                                                                                                                        gTCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTACAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTGGATAAAAAGGTa               >  2:571718/1‑149 (MQ=255)

                                                                                                                         tCTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGCCGATAAAAAGGTaa              >  1:832714/1‑149 (MQ=255)

                                                                                                                          cTAATCAATCAAAAAGGGCATGATAGTTCTGTCCAAAAAGCACTATCACTCGGACAATCAAACGATCGATTAATTAGAAAAGATTATGGCGGTGGTAAGCACCGCGTCTGGGTATATAATATCCCAGGTAAAGTGGGTAAAAAGGTact             >  2:1032862/1‑147 (MQ=255)

                                                                                                                          cTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAAt             >  2:92651/1‑149 (MQ=255)

                                                                                                                          cTAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAAt             >  2:1196216/1‑149 (MQ=255)

                                                                                                                           tAATCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGATCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAAtt            <  1:851118/149‑1 (MQ=255)

                                                                                                                           tAACCAATCAAAAAGGGAATGATAGTTCTGTCCAAAAAGCATTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAAtt            <  2:901787/149‑1 (MQ=255)

                                                                                                                            aatcaaccAAAAAGGTAATGATAGTTCTGTCCAAAAATCACTACCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTCAGGACCGTGTCTGGGTATATAATATCCCAGTTATAGTCGATCAAAAGGTAATTg           <  1:947468/149‑1 (MQ=255)

                                                                                                                             atcaatcaAAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTGTGGACACGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTgg          >  1:976917/1‑149 (MQ=255)

                                                                                                                              tcaatcaaAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGCCCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGt         <  2:717400/149‑1 (MQ=255)

                                                                                                                              acaatcaaAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGt         <  2:296384/148‑1 (MQ=255)

                                                                                                                              aaaatcaaAAAGGGAATGATAGTTATGTCCAAAAAGCACTATCACTAGGACAATCAATCGATCATTTAATTTTAAAAGATTATGGCTGTGGTAAGGACCGTGTCTGGGTATATAATATCCCCGTTAAAGTCGATAAAAAGGTAATTGGt         <  1:872323/147‑1 (MQ=255)

                                                                                                                               aaatcaaAAAGGGGATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTa        <  2:303386/148‑1 (MQ=255)

                                                                                                                               aaatcaaAAAGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTa        <  1:13472/148‑1 (MQ=255)

                                                                                                                                    aaaaaGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTAATAtt   <  2:536047/149‑1 (MQ=255)

                                                                                                                                     aaaaGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCGAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTAGTAAAAttt  >  1:960359/1‑149 (MQ=255)

                                                                                                                                     aaaaGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCACACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTAATAttt  >  1:1266739/1‑149 (MQ=255)

                                                                                                                                     aaaaGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTaaagttt  >  2:1338123/1‑144 (MQ=255)

                                                                                                                                     aaaaGGGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTAATAttt  >  2:1202927/1‑149 (MQ=255)

ATGCACAAAAAGATATTCAAAATTTATTAAGTGAGTATGCCAACCGTCAAGAAATTGGAGAAATTCGTTTTATAGATAAAGACCAAATTATTATTGCGACGACGAAGCAGTCTAACCGTAGTCTAATCAATCAAAAAGCGAATGATAGTTCTGTCCAAAAAGCACTATCACTAGGACAATCAAACGATCATTTAATTTTAAAAGATTATGGCGGTGGTAAGGACCGTGTCTGGGTATATAATATCCCAGTTAAAGTCGATAAAAAGGTAATTGGTAATATTT  >  USA300TCH1516_ALE/25877‑26158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`