Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	973,213	C→A	E175* (GAG→TAG)	yitU_1 ←	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	973,213	0	C	A	100.0%	307.6 / NA	87	E175* (GAG→TAG)	yitU_1	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/35);  total (52/35)

ATATCATTATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGAAAAA  >  USA300TCH1516_ALE/973068‑973358                                                                                                                                                   |                                                                                                                                                  atatCATTATCTTCATCACCGAATGCAATAATATCATCACTGTTAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAat                                                                                                                                                >  2:1232997/1‑149 (MQ=255) atatCATTATCTTCATCACCGAATGCAATAAGATTATTTCGGTCAATATTTAAAAATTCTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGAGAATG‑GTGCGCCCCAGCGTCGATGCTAAat                                                                                                                                                >  2:988413/1‑149 (MQ=255)        tATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCg                                                                                                                                         <  1:655695/149‑1 (MQ=255)          tCTTCATCACCGAATGCAATAAGATTATTTCGGTCAATATATAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTTCGCCCCAGCGTCGATGCTAAATATGATCGGc                                                                                                                                       <  2:683854/149‑1 (MQ=255)           cTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCa                                                                                                                                      <  1:1242404/149‑1 (MQ=255)               atcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaa                                                                                                                                  >  1:939499/1‑149 (MQ=255)                 caCCGAAAGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCACAGCGTCGATGCTAAATATGATCGGCATaaaaa                                                                                                                                <  1:673512/149‑1 (MQ=255)                      aaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGt                                                                                                                           <  2:467637/149‑1 (MQ=255)                        tGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTaa                                                                                                                         <  1:666935/149‑1 (MQ=255)                                     tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAt                                                                                                            >  2:1244781/1‑149 (MQ=255)                                     tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGACATAAAAATGAGTAAGCATAGTTTTGAt                                                                                                            >  2:541108/1‑149 (MQ=255)                                       tCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTTAttt                                                                                                          >  2:1038475/1‑149 (MQ=255)                                          gTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAg                                                                                                       >  1:1338585/1‑149 (MQ=255)                                          gTCAATATTTAAAAATTGTCTAACTTGCTCAATATTAAAATCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATGTTTTTGATTTCAg                                                                                                       >  1:648809/1‑149 (MQ=255)                                           tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTGCAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                      >  2:369697/1‑149 (MQ=255)                                           tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCCTCTTTTTGAGTTCAgg                                                                                                      >  2:1213598/1‑149 (MQ=255)                                           tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                      >  2:645267/1‑149 (MQ=255)                                           tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                      >  1:468794/1‑149 (MQ=255)                                           tCAATATTTAAAAATCGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                      >  1:842406/1‑149 (MQ=255)                                            cAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATCCCACGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAAGt                                                                                                     >  1:588070/1‑149 (MQ=255)                                            cAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGt                                                                                                     >  2:271143/1‑149 (MQ=255)                                               tctttAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                                  <  1:878681/147‑1 (MQ=255)                                               tatTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                                  <  1:1284260/149‑1 (MQ=255)                                               tatTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGAGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                                  <  1:931320/149‑1 (MQ=255)                                                       aaTTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttc                                                                                          >  1:604161/1‑149 (MQ=255)                                                            tCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                                     >  2:724963/1‑149 (MQ=255)                                                             cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGc                                                                                    >  2:854540/1‑149 (MQ=255)                                                                aCTTGCTCAATGCCTCTTGCTTTATTAGTACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGGATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTc                                                                                 >  1:751334/1‑149 (MQ=255)                                                                 cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                                >  1:1094633/1‑149 (MQ=255)                                                                  ttGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCACCAGCGTGGGTGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCaa                                                                               >  1:1189476/1‑149 (MQ=255)                                                                   tGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCCAt                                                                              >  2:137754/1‑149 (MQ=255)                                                                   tGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAAt                                                                              >  2:520862/1‑149 (MQ=255)                                                                   tGCTCAATGCCTCTGGCTTTATTAATACCTAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAAt                                                                              <  2:572423/149‑1 (MQ=255)                                                                      tCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaa                                                                           <  1:724963/149‑1 (MQ=255)                                                                       cAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaa                                                                          >  2:756109/1‑149 (MQ=255)                                                                       cAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTAATTTCTTCGGCTTCAATTaaa                                                                          >  2:1239460/1‑149 (MQ=255)                                                                         aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAAt                                                                        <  1:1137835/149‑1 (MQ=255)                                                                           tccTCTTGCTTGATTAGTACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                      <  2:571624/148‑1 (MQ=255)                                                                           gCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                      <  1:1245266/149‑1 (MQ=255)                                                                           gCCTCTTGCTTTATTAATACCAAGTTTTACAATGTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                      <  1:306337/149‑1 (MQ=255)                                                                           gCCTCTTGCGTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                                      <  2:555811/149‑1 (MQ=255)                                                                            ccTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGa                                                                     <  2:375038/149‑1 (MQ=255)                                                                                     ttattaATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATAAGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                            >  1:910192/1‑149 (MQ=255)                                                                                     ttattaATACCAAGTTTTACAATTTCAATGACAGGAAATGTGTGTG‑GCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                            >  1:320123/1‑149 (MQ=255)                                                                                          aaTACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTCAGGTAGGGGATTCtt                                                       >  2:918736/1‑149 (MQ=255)                                                                                           aTACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCttt                                                      >  2:17987/1‑149 (MQ=255)                                                                                              ccAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGCTTCTTTCaa                                                   >  2:242663/1‑149 (MQ=255)                                                                                              ccAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                                   <  1:227586/149‑1 (MQ=255)                                                                                              ccAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGATTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                                   >  1:177589/1‑149 (MQ=255)                                                                                              ccAAGGTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                                   <  1:599299/149‑1 (MQ=255)                                                                                               cAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCCACGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGCTTTCAGGTATTTTACTTTCTTCGGCTTCCATTAAAATTGGGGTAGGGGGTTCTTTCAAg                                                  >  1:56206/1‑149 (MQ=255)                                                                                               cAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGCTCGGCATAAAAATGAGTAAGCATATTTTTGATTTCCGGTATTTTGCTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                                  >  2:991531/1‑149 (MQ=255)                                                                                                aaGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGt                                                 >  2:790992/1‑149 (MQ=255)                                                                                                aaGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGt                                                 >  1:545389/1‑149 (MQ=255)                                                                                                aaGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGt                                                 >  1:796902/1‑149 (MQ=255)                                                                                                 aGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTg                                                >  2:34659/1‑149 (MQ=255)                                                                                                   ttttACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGa                                              <  2:1066935/149‑1 (MQ=255)                                                                                                   ttttACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTCGa                                              <  2:682150/149‑1 (MQ=255)                                                                                                   ttttACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCCATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGa                                              <  1:596667/149‑1 (MQ=255)                                                                                                    tttACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGAc                                             <  1:756926/149‑1 (MQ=255)                                                                                                    tttACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGAc                                             <  2:257250/149‑1 (MQ=255)                                                                                                    tttACAATTTCAACGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGAc                                             <  1:724117/149‑1 (MQ=255)                                                                                                    tttACAATTGCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGAc                                             <  2:1271132/149‑1 (MQ=255)                                                                                                    tttAAAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGAc                                             <  2:327762/149‑1 (MQ=255)                                                                                                      tACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAGCTTGAGGTAGGGGATTCTTTTAAGTTGActt                                           >  2:471476/1‑147 (MQ=255)                                                                                                      tACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACaa                                           >  2:144691/1‑149 (MQ=255)                                                                                                       aCAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAg                                          <  1:854540/149‑1 (MQ=255)                                                                                                              cAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTa                                   >  2:307711/1‑149 (MQ=255)                                                                                                              cAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTa                                   >  1:284648/1‑149 (MQ=255)                                                                                                               aaTGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTAc                                  <  1:289216/149‑1 (MQ=255)                                                                                                                aTGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTAcc                                 >  2:1223573/1‑149 (MQ=255)                                                                                                                   aCAGGGAATG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGt                              >  2:1113122/1‑149 (MQ=255)                                                                                                                    cAGGGAATG‑GCGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGtt                             <  1:1068765/149‑1 (MQ=255)                                                                                                                           tG‑GTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGTTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTc                      <  1:396648/149‑1 (MQ=255)                                                                                                                              gTGCGCCCCAGCGTCGATGCTAAATATGATTGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTGtt                    >  1:338836/1‑149 (MQ=255)                                                                                                                              gTGCGCCCCAGCGTCGATGCTAAATATGATTGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATGGTGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATtttt                    >  1:372661/1‑149 (MQ=255)                                                                                                                              gTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGGTTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCtt                    >  1:1324088/1‑149 (MQ=255)                                                                                                                              gTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCtt                    >  1:1324064/1‑149 (MQ=255)                                                                                                                              gTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGGTTGAATTCtt                    >  1:224817/1‑149 (MQ=255)                                                                                                                                   ccccAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGAtt               <  2:579912/149‑1 (MQ=255)                                                                                                                                   ccccAGCGTCGATGCTAAATATGATCGACATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGAtt               <  1:105176/149‑1 (MQ=255)                                                                                                                                     ccAGCGGCGATGCTAAATATGATCGGCATAAGAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAc             <  1:319701/149‑1 (MQ=255)                                                                                                                                         cggcGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCAt         >  2:1183691/4‑149 (MQ=255)                                                                                                                                         cGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCAt         >  1:484225/1‑149 (MQ=255)                                                                                                                                             gATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCCTTGaa     >  2:255106/1‑149 (MQ=255)                                                                                                                                             gATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGCATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGCTTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGaa     >  2:736042/1‑149 (MQ=255)                                                                                                                                                gCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGaaaaa  <  1:17987/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATATCATTATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATG‑GTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGAAAAA  >  USA300TCH1516_ALE/973068‑973358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑