New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	94 (0.750)	58 (0.650) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	15/194	7.2	45.7%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
	?	USA300TCH1516_ALE	1426517 =	98 (0.780)					intergenic (‑248/+724)	pstS/cvfB	Phosphate‑binding protein PstS/Conserved virulence factor B
Rejected: Coverage evenness skew score above cutoff.

ATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGT  >  USA300TCH1516_ALE/1426380‑1426656                                                                                                                                          |                                                                                                                                              aTTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAca                                                                                                                                            >  2:485199/1‑141 (MQ=255)    gTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagca                                                                                                                                         >  1:835607/1‑141 (MQ=255)     ttAAGAAAATGTAAATATTAATTTTTGTTTATTTTAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAaaccag                                                                                                                                        >  2:807612/1‑141 (MQ=255)     ttAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagcag                                                                                                                                        >  2:791229/1‑141 (MQ=255)     ttAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagcag                                                                                                                                        >  3:314638/1‑141 (MQ=255)       aaGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTa                                                                                                                                      >  2:945806/1‑141 (MQ=255)          aaaaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGa                                                                                                                                   <  3:299136/141‑1 (MQ=255)           aaaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAt                                                                                                                                  <  2:297812/141‑1 (MQ=255)           aaaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAt                                                                                                                                  <  1:60526/141‑1 (MQ=255)             aTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATaa                                                                                                                                <  2:735238/141‑1 (MQ=255)             aTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATaa                                                                                                                                >  2:504059/1‑141 (MQ=255)                tAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTACAAAAACAGCAGTAAGATAAttt                                                                                                                             >  4:223187/1‑141 (MQ=255)                 aaaTATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAtttt                                                                                                                            >  4:120272/1‑141 (MQ=255)                  aaTATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTc                                                                                                                           >  1:528367/1‑141 (MQ=255)                    tatTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑aa                                                                                                                        <  3:391649/141‑1 (MQ=255)                      ttAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AAtt                                                                                                                      <  1:290340/141‑1 (MQ=255)                          tttttGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGaa                                                                                                                  >  1:626802/1‑141 (MQ=255)                               gTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAa‑ttat                                                                                                           <  1:581944/141‑4 (MQ=38)                                ttAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATc                                                                                                           >  1:93781/1‑141 (MQ=255)                                   aTCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTa                                                                                                        >  1:661942/1‑141 (MQ=255)                                       aaaaaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActgc                                                                                                    <  2:709325/141‑1 (MQ=255)                                       aaaaaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActgc                                                                                                    <  3:20704/141‑1 (MQ=255)                                       aaaaaaTATTGCTTCATTAATAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActgc                                                                                                    <  2:709202/141‑1 (MQ=255)                                        aaaaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAT‑ATTTTCTAATTGAAAAT‑TATCTTActgct                                                                                                   <  2:1007643/141‑1 (MQ=37)                                        aaaaaGATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAANAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATATTTTCT‑AATT‑GAAAATTATCNTActgct                                                                                                   <  2:904607/141‑1 (MQ=25)                                          aaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGt                                                                                                 <  3:376878/141‑1 (MQ=255)                                            atatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtc                                                                                               <  4:29041/141‑1 (MQ=255)                                            atatTACATCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAAAAAAAAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtc                                                                                               <  2:970583/141‑1 (MQ=25)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  3:252371/141‑1 (MQ=255)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  1:176924/141‑1 (MQ=255)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  1:178024/141‑1 (MQ=255)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  3:343911/141‑1 (MQ=255)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  3:143699/141‑1 (MQ=255)                                              atTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtctc                                                                                             <  2:588602/141‑1 (MQ=255)                                               ttGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTctct                                                                                            >  2:758960/1‑141 (MQ=255)                                                tGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTa                                                                                           <  2:521437/141‑1 (MQ=255)                                                       ttAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATAc                                                                                    <  1:606792/141‑1 (MQ=255)                                                         aGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACaa                                                                                  <  2:844311/141‑1 (MQ=255)                                                         aGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACaa                                                                                  <  1:832871/141‑1 (MQ=255)                                                          ataaaattaaaatGCTTCCTAAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTAGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAAt                                                                                 <  2:124138/140‑1 (MQ=255)                                                           taaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑ACTTAGAAAA‑GATCTTACTGCTGTTCTCTATTTATACAATa                                                                                <  1:28087/141‑1 (MQ=255)                                                           taaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATa                                                                                <  3:85822/141‑1 (MQ=255)                                                           aaaaattaaaatGCTTACTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCATAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATa                                                                                <  2:170051/140‑1 (MQ=255)                                                            aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATAc                                                                               >  1:606858/1‑141 (MQ=255)                                                               attaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTc                                                                            <  3:203566/141‑1 (MQ=255)                                                               attaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTc                                                                            >  4:16286/1‑141 (MQ=255)                                                               attaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTc                                                                            <  1:632735/141‑1 (MQ=255)                                                                ttaaaatGCTTCATGAAAAAACATAGAAATTTAAATATGAATACAAAAATGGCTAGGACATAAATCACTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCg                                                                           <  4:324267/141‑1 (MQ=18)                                                                    aatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagca                                                                                                                                         >  2:479979/1‑77 (MQ=255)                                                                    aatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAAcagca                                                                                                                                         <  1:479979/77‑1 (MQ=255)                                                                       gCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGaa                                                                    <  1:791229/141‑1 (MQ=255)                                                                       gCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGaa                                                                    >  1:882527/1‑141 (MQ=255)                                                                       gCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGaa                                                                    >  4:180462/1‑141 (MQ=255)                                                                        cTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAAt                                                                   <  2:157395/141‑1 (MQ=255)                                                                         ttCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATg                                                                  >  3:327323/1‑141 (MQ=255)                                                                          tCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATgg                                                                 <  2:902762/141‑1 (MQ=255)                                                                           ccTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATACCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGc                                                                <  4:293046/141‑1 (MQ=255)                                                                            cTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCt                                                               >  3:249449/1‑141 (MQ=255)                                                                              gAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTc                                                             >  2:545444/1‑141 (MQ=255)                                                                              gAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACCGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTc                                                             <  1:621603/141‑1 (MQ=255)                                                                                  aaTCATCGAAATTTAAATATGAATACAAAAATTGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTGCTTATTGAATGGCTTCGCtt                                                         <  1:668344/141‑1 (MQ=255)                                                                                      atcGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTCTACAATACTTCGTATTGAATGGCTTAGCtttcct                                                     >  2:63829/1‑138 (MQ=255)                                                                                      atcGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGcc                                                     <  1:1020983/141‑1 (MQ=255)                                                                                      atcGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGcc                                                     <  2:305336/141‑1 (MQ=255)                                                                                           aaTTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCt                                                <  4:115581/141‑1 (MQ=255)                                                                                           aaTTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCt                                                <  3:440020/141‑1 (MQ=255)                                                                                             tttAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGt                                              <  1:391740/141‑1 (MQ=255)                                                                                              ttAAATATGAATACAACAATGGCAGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTGCTCTATTTATACAATACTTCGTAGTGAATGGCTTCGCTTTGCCCGTCTGTc                                             <  1:736362/141‑1 (MQ=255)                                                                                                aaaTATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTCGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGCCTTCGCTTTGCCCGTCTGTcac                                           >  2:307201/1‑141 (MQ=255)                                                                                                aaaTATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTcac                                           <  3:240932/141‑1 (MQ=255)                                                                                                  atatGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACAt                                         <  1:509303/141‑1 (MQ=255)                                                                                                     tGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTAc                                      <  2:615464/141‑1 (MQ=255)                                                                                                       aaTACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTg                                    <  1:721606/141‑1 (MQ=255)                                                                                                       aaTACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTg                                    <  2:36531/141‑1 (MQ=255)                                                                                                         tACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTa                                  <  1:303407/141‑1 (MQ=255)                                                                                                           cAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTaaa                                <  2:70271/141‑1 (MQ=255)                                                                                                              aaaTGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAAtt                             <  3:120272/141‑1 (MQ=255)                                                                                                                  ggCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACCTTACTGTAAAATTCtat                         >  3:14263/1‑141 (MQ=255)                                                                                                                  ggCTGGGACATAAATACCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCGGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCtat                         <  1:856587/141‑1 (MQ=255)                                                                                                                     tGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACAtt                                        <  1:828283/123‑1 (MQ=255)                                                                                                                     tGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACAtt                                        >  2:828283/1‑123 (MQ=255)                                                                                                                      gggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAAt                     <  1:1016165/141‑1 (MQ=255)                                                                                                                      gggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAAt                     <  1:55412/141‑1 (MQ=255)                                                                                                                        gACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCtatttatag                   <  1:193955/141‑7 (MQ=255)                                                                                                                        gACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAg                   <  3:257646/141‑1 (MQ=255)                                                                                                                        gACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAg                   <  2:93781/141‑1 (MQ=255)                                                                                                                        gACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAg                   <  1:13199/141‑1 (MQ=255)                                                                                                                         aCATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGa                  <  1:807612/141‑1 (MQ=255)                                                                                                                             aaaTCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt              <  1:838532/141‑1 (MQ=255)                                                                                                                              aaTCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAtttt             <  4:309298/141‑1 (MQ=255)                                                                                                                                tCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtg           <  2:902193/141‑1 (MQ=255)                                                                                                                                 cccTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt              <  1:373425/137‑1 (MQ=255)                                                                                                                                 cccTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt              >  2:373425/1‑137 (MQ=255)                                                                                                                                  ccTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtgat         <  2:373502/141‑1 (MQ=255)                                                                                                                                   cTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtgatg        <  1:146033/141‑1 (MQ=40)                                                                                                                                      aaaaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACg     <  1:945806/141‑1 (MQ=39)                                                                                                                                        aaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACggg   >  2:730715/1‑141 (MQ=17)                                                                                                                                         aaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTAAATAACTTCGCTTTGCCCGTCTGTCACATTACTTTAAAATTCTATAAATAGAATTTTTGATTACGGGt  >  4:100978/1‑141 (MQ=11)                                                                                                                                          |                                                                                                                                              ATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATACAAAAATGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGT  >  USA300TCH1516_ALE/1426380‑1426656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.