BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	94 (0.750)	59 (0.660) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	16/194	6.9	43.5%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
*	?	USA300TCH1516_ALE	= 2072043	119 (0.950)	59 (0.660) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	16/194	6.9	43.5%	intergenic (+538/+35)	USA300HOU_RS10445/USA300HOU_RS10450	hypothetical protein/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.

Only 100 of 120 total aligned reads displayed.

AGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTACACC  >  USA300TCH1516_ALE/2071903‑2072176

agaCTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGtt                                                                                                                                       >  3:258890/1‑141 (MQ=32)

   cTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGTAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGttttt                                                                                                                                    <  2:519038/141‑1 (MQ=17)

   cTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGttttt                                                                                                                                    <  2:518689/141‑1 (MQ=32)

   cTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGtcttt                                                                                                                                    >  4:358878/1‑141 (MQ=17)

     ccTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTa                                                                                                                                  <  4:45915/141‑1 (MQ=32)

      cTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAg                                                                                                                                 >  2:401646/1‑141 (MQ=32)

       tGAGGGAGCAGTGCCAGTCGAAGAAGAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAgg                                                                                                                                >  2:46060/1‑141 (MQ=17)

        gAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAggg                                                                                                                               >  1:736923/1‑141 (MQ=32)

           cgtgCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTCCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGAtt                                                                                                                            <  2:119655/138‑1 (MQ=17)

             aGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTa                                                                                                                          <  2:409597/141‑1 (MQ=32)

              gCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTAt                                                                                                                         >  3:378604/1‑141 (MQ=32)

               cAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATg                                                                                                                        >  3:412394/1‑141 (MQ=32)

                aGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGt                                                                                                                       <  3:170896/141‑1 (MQ=32)

                    ccAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCa                                                                                                                   <  2:789049/141‑1 (MQ=32)

                    ccAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCa                                                                                                                   >  4:141125/1‑141 (MQ=32)

                     cAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAg                                                                                                                  <  3:348225/141‑1 (MQ=32)

                            aGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTaa                                                                                                           <  1:324218/141‑1 (MQ=34)

                            aGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTaa                                                                                                           <  1:357767/141‑1 (MQ=34)

                               ccAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATg                                                                                                        >  2:31294/1‑141 (MQ=35)

                               ccAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATg                                                                                                        <  3:288624/141‑1 (MQ=35)

                                cAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGt                                                                                                       <  4:106702/141‑1 (MQ=35)

                                 aaGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGtt                                                                                                      <  2:35834/141‑1 (MQ=35)

                                  aGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGtta                                                                                                     <  2:502734/141‑1 (MQ=35)

                                   ggCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCATCCTCTAAATGttat                                                                                                    >  4:408380/1‑141 (MQ=37)

                                     cTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGttatta                                                                                                  >  2:661256/1‑141 (MQ=38)

                                         gaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTa                                                                                              >  1:428238/1‑141 (MQ=255)

                                         gaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTa                                                                                              >  1:242904/1‑141 (MQ=255)

                                          aCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTATTTTTTTATGTCCCAGCCTCTAAATGTTATTAGTTAt                                                                                             >  4:410615/1‑141 (MQ=255)

                                          aCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTAt                                                                                             >  2:401388/1‑141 (MQ=255)

                                              cACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTAttttt                                                                                         <  3:78606/141‑1 (MQ=255)

                                               aCCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTCTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTa                                                                                        <  3:358878/141‑1 (MQ=255)

                                                 ccTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACt                                                                                      >  3:353747/1‑141 (MQ=255)

                                                     ggAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCa                                                                                  <  1:558584/141‑1 (MQ=255)

                                                      gAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCaa                                                                                 <  1:46060/141‑1 (MQ=255)

                                                      gAAAGCGAAGCCATTAAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCaa                                                                                 <  4:184887/141‑1 (MQ=255)

                                                         aGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAatat                                                                              <  3:378785/141‑1 (MQ=255)

                                                          gCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATc                                                                             <  2:78130/141‑1 (MQ=255)

                                                           cGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCa                                                                            >  2:613372/1‑141 (MQ=255)

                                                            gAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAg                                                                           <  3:117339/141‑1 (MQ=255)

                                                             aaGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGa                                                                          <  4:258890/141‑1 (MQ=255)

                                                             aaGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGa                                                                          >  1:672051/1‑141 (MQ=255)

                                                              aGCCATTCAATACGAAGTATTGTATAAATAGCGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGaa                                                                         <  1:997211/141‑1 (MQ=255)

                                                                ccATTCAATACTAAGTATTGTATTTATTGATAATAGCATTATGAT‑TTTTTTTAATT‑GAAATTTCTCTTACTGCTTTTTTTTAGTGATTTATGTCCAAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAaat                                                                       <  2:469297/141‑1 (MQ=255)

                                                                      aaTACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTc                                                                 <  1:392994/141‑1 (MQ=255)

                                                                       aTACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCt                                                                <  3:156569/141‑1 (MQ=255)

                                                                        tACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCtt                                                               <  1:633938/141‑1 (MQ=255)

                                                                        tACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCtt                                                               <  1:672244/141‑1 (MQ=255)

                                                                         aCGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCttt                                                              >  2:696989/1‑141 (MQ=255)

                                                                         aCGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCttt                                                              <  4:353747/141‑1 (MQ=255)

                                                                           gAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTTCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAg                                                            <  3:120069/141‑1 (MQ=255)

                                                                            aaGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGt                                                           <  2:935017/141‑1 (MQ=255)

                                                                            aaGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGt                                                           >  1:764859/1‑141 (MQ=255)

                                                                             aGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGtt                                                          <  3:295844/141‑1 (MQ=255)

                                                                              gTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGttt                                                         <  2:950658/141‑1 (MQ=255)

                                                                               tATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTTTTTAGtttt                                                        >  3:49027/1‑141 (MQ=255)

                                                                               tATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGtttt                                                        <  1:950424/141‑1 (MQ=255)

                                                                                 ttGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttc                                                      >  4:426044/1‑141 (MQ=255)

                                                                                  tGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttct                                                     <  2:23716/141‑1 (MQ=255)

                                                                                     ataAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttctttc                                                  >  1:78853/1‑141 (MQ=255)

                                                                                        aaTAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAat                                               <  4:348782/141‑1 (MQ=255)

                                                                                        aaTAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTGACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAat                                               >  1:463230/1‑141 (MQ=255)

                                                                                         aTAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAata                                              >  1:696718/1‑141 (MQ=255)

                                                                                           agagAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATc                                            <  1:441046/141‑1 (MQ=255)

                                                                                               aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCa                                        >  2:418765/1‑141 (MQ=255)

                                                                                                 cagcagTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTGTTTCAGTATCTTCaaa                                      >  3:247104/1‑141 (MQ=255)

                                                                                                   gcagTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAAt                                    >  4:291097/1‑141 (MQ=37)

                                                                                                   gcagTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGttatt                                                                                                   >  4:117145/1‑78 (MQ=255)

                                                                                                    cagTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATc                                   <  3:403270/141‑1 (MQ=255)

                                                                                                    cagTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATc                                   <  2:485397/141‑1 (MQ=255)

                                                                                                    cagTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATc                                   <  1:141881/141‑1 (MQ=255)

                                                                                                    aagTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATc                                   <  3:171259/140‑1 (MQ=255)

                                                                                                      gTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCtt                                 >  1:19896/1‑141 (MQ=255)

                                                                                                        aaGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCTTTTTCTTCAAAATCtttt                               >  2:560143/1‑141 (MQ=255)

                                                                                                        aaGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCtttt                               >  4:230682/1‑141 (MQ=255)

                                                                                                           at‑atTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCACTATCAGAAATAATTTCTTTAGTTTTTCTTTCACTATCTTCAAAATCTTTTTTa                            >  2:603423/1‑141 (MQ=255)

                                                                                                              atTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTaaa                          >  3:85782/1‑141 (MQ=255)

                                                                                                               ttttCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCttt                                <  4:341233/134‑1 (MQ=255)

                                                                                                                tttCTTATT‑GAATATTGTCTTACTGCTGTTTTTTAGGGATTTATGTCCGAGCCTCTAGATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGa                        <  2:191657/141‑1 (MQ=38)

                                                                                                                tttCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGa                        <  2:581116/141‑1 (MQ=255)

                                                                                                                 ttCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTATTTTTATTATTTTAAATAATGTCTTTAGTTTTTCTTTTAATTTCTTCAAATTCTTTTTTAAAGat                       >  4:50904/1‑141 (MQ=25)

                                                                                                                 ttCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGat                       >  1:334888/1‑141 (MQ=255)

                                                                                                                    tAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGAtata                    <  1:66912/141‑1 (MQ=255)

                                                                                                                    tAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGAtata                    <  4:240386/141‑1 (MQ=255)

                                                                                                                    tAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGAtata                    <  2:130242/141‑1 (MQ=255)

                                                                                                                      aTT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAg                  <  1:130147/141‑1 (MQ=255)

                                                                                                                      aTT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAg                  <  2:896420/141‑1 (MQ=255)

                                                                                                                      aTT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAg                  <  1:853338/141‑1 (MQ=255)

                                                                                                                         agAAAA‑TATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttctttc                                                  >  1:128860/2‑106 (MQ=255)

                                                                                                                         agAAAA‑TATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTtttctttc                                                  <  2:128860/105‑1 (MQ=255)

                                                                                                                          gAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCaa               >  1:392727/1‑141 (MQ=255)

                                                                                                                            aaaTTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAAt             >  3:46901/1‑141 (MQ=255)

                                                                                                                                 aTCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGctac        <  2:392727/141‑1 (MQ=255)

                                                                                                                                 aTCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGctac        >  1:809606/1‑141 (MQ=255)

                                                                                                                                 aTCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGctac        >  2:556342/1‑141 (MQ=255)

                                                                                                                                   cTTACTGCTGTTTTTTTTTTTTTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGctacta      >  2:443272/1‑141 (MQ=255)

                                                                                                                                     tACTGCTGTTTTTTAGGTATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTaca    <  2:455371/141‑1 (MQ=255)

                                                                                                                                     tACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTTTTATTCTTTTATGAAAATACAAGCAAAGGCTACTaca    <  4:320181/141‑1 (MQ=38)

                                                                                                                                      aCTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGACATAAGCAAATGCTACTacac   <  3:81659/141‑1 (MQ=255)

                                                                                                                                       ctgctgTTTTTTATGGATTTATGTCCTAGCCTCTACATGTTATTAGTTATTTTTACTTTCAATATATGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTacacc  >  2:324286/1‑141 (MQ=255)

                                                                                                                                       ctgctgTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTacacc  <  3:253202/141‑1 (MQ=255)

AGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCAAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTAGGGATTTATGTCCCAGCCTCTAAATGTTATTAGTTATTTTTACTTTCAATATCAGAAATAATTTCTTTAGTTTTTCTTTCAATATCTTCAAAATCTTTTTTAAAGATATAAGCAAATGCTACTACACC  >  USA300TCH1516_ALE/2071903‑2072176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.