Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,232,052 G→T D233Y (GAT→TAT)  stp → Serine/threonine phosphatase stp

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,232,0520GT94.6% 358.3 / 11.9 112D233Y (GAT→TAT) stpSerine/threonine phosphatase stp
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (45/61);  total (46/66)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 112 total aligned reads displayed.

TATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGAT  >  USA300TCH1516_ALE/1231917‑1232187
                                                                                                                                       |                                                                                                                                       
tatttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAAc                                                                                                                                    <  3:58614/141‑1 (MQ=255)
 atttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACg                                                                                                                                   <  2:42999/141‑1 (MQ=255)
  tttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATaaagc                                                                                                                                  <  2:574128/141‑4 (MQ=255)
        attaAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACttt                                                                                                                            >  1:532380/1‑141 (MQ=255)
         ttaAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTc                                                                                                                           <  2:893309/141‑1 (MQ=255)
         ttaAAGTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTGTGTTAGTAACAGAAGGTACAATAGAAGATCATGGTGATCAATTGATGCAATTGGCATTAGATACCCATTCGAAGTATAACGTTACCTTc                                                                                                                           <  2:170749/141‑1 (MQ=255)
           aaaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCAt                                                                                                                         >  2:603162/1‑141 (MQ=255)
           aaaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCAt                                                                                                                         <  4:171678/141‑1 (MQ=255)
           aaaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCAt                                                                                                                         <  4:323815/141‑1 (MQ=255)
            aaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATa                                                                                                                        >  2:681448/1‑141 (MQ=255)
             aTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATAc                                                                                                                       >  1:714469/1‑141 (MQ=255)
                 aGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgc                                                                                                                   <  1:198353/141‑1 (MQ=255)
                  gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgcg                                                                                                                  >  2:99647/1‑141 (MQ=255)
                  gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgcg                                                                                                                  <  1:753039/141‑1 (MQ=255)
                  gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgcg                                                                                                                  >  1:565739/1‑141 (MQ=255)
                  gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTcgcg                                                                                                                  <  4:121662/141‑1 (MQ=255)
                   aTGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg                                                                                                                 <  1:533460/141‑1 (MQ=255)
                    tGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGc                                                                                                                <  1:176717/141‑1 (MQ=255)
                    tGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGc                                                                                                                <  1:334442/141‑1 (MQ=255)
                     ggATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCt                                                                                                               >  1:272563/1‑141 (MQ=255)
                     ggATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCt                                                                                                               <  1:739440/141‑1 (MQ=255)
                     ggATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCt                                                                                                               >  2:504857/1‑141 (MQ=255)
                      gATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTa                                                                                                              <  2:895364/141‑1 (MQ=255)
                      gATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTa                                                                                                              <  2:243489/141‑1 (MQ=255)
                             tGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAgg                                                                                                       <  1:628302/141‑1 (MQ=255)
                              gATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGt                                                                                                      >  2:1009456/1‑141 (MQ=255)
                              gATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGt                                                                                                      <  2:463863/141‑1 (MQ=255)
                              aaTTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGAACATGGTGATCAATTAATGCCATTGGCATTAGATAACCATGCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAAGt                                                                                                      <  4:172677/140‑1 (MQ=255)
                               aTTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTg                                                                                                     <  4:417698/141‑1 (MQ=255)
                                ttATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGa                                                                                                    <  3:132025/141‑1 (MQ=255)
                                 tATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGAt                                                                                                   <  3:152546/141‑1 (MQ=255)
                                  aTGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATa                                                                                                  <  2:272563/141‑1 (MQ=255)
                                  aTGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATa                                                                                                  <  2:677881/141‑1 (MQ=255)
                                  aTGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATa                                                                                                  <  1:504857/141‑1 (MQ=255)
                                    gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATaaa                                                                                                >  3:201347/1‑141 (MQ=255)
                                    gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATaaa                                                                                                >  3:315551/1‑141 (MQ=255)
                                    gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATaaa                                                                                                >  2:932600/1‑141 (MQ=255)
                                     ttAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAg                                                                                               >  1:777889/1‑141 (MQ=255)
                                      tAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGAGCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGt                                                                                              <  1:65863/141‑1 (MQ=255)
                                       aaaGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTa                                                                                             >  1:435674/1‑141 (MQ=255)
                                         aGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATg                                                                                           >  1:633642/1‑141 (MQ=255)
                                           aCAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGAt                                                                                         <  4:410453/141‑1 (MQ=255)
                                              aTGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAgg                                                                                      >  4:418150/1‑141 (MQ=255)
                                                   aTTAAGCGTTTGTTAGTAAAAGAAGGTAAAATAAAAGAACATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTaaaa                                                                                 <  2:464532/141‑1 (MQ=255)
                                                     tAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaata                                                                               <  4:228010/141‑1 (MQ=255)
                                                      aaGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataa                                                                              <  1:872767/141‑1 (MQ=255)
                                                       aGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg                                                                                                                 >  1:552190/1‑105 (MQ=255)
                                                       aGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg                                                                                                                 <  2:552190/105‑1 (MQ=255)
                                                       aGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataat                                                                             <  1:981789/141‑1 (MQ=255)
                                                                tAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACg                                                                    <  1:304023/141‑1 (MQ=255)
                                                                 aGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGa                                                                   <  1:804656/141‑1 (MQ=255)
                                                                 aGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGa                                                                   <  2:797339/141‑1 (MQ=255)
                                                                    aaaaGAAGGAACCACAGATGTTCATGGTACACCATCAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTAATTTCATACTCGCGGCTATTGAAGGTGCTATAGTCTGATAGGTAAACTAATACATGAACGatat                                                                <  2:616638/141‑1 (MQ=255)
                                                                     aaaGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGAtata                                                               <  2:364910/141‑1 (MQ=255)
                                                                       agaagGTACAATAGAAGATCATGGTGCTCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaa                                                             >  2:730058/1‑141 (MQ=255)
                                                                       agaagGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaa                                                             >  4:182601/1‑141 (MQ=255)
                                                                       agaagGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaa                                                             <  1:681448/141‑1 (MQ=255)
                                                                          agGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAAtt                                                          >  1:1025618/1‑141 (MQ=255)
                                                                                 aTAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAATGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATa                                                   >  2:858740/1‑141 (MQ=255)
                                                                                  tAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATaa                                                  >  1:291351/1‑141 (MQ=255)
                                                                                   agaagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAg                                                 >  1:899188/1‑141 (MQ=255)
                                                                                     aagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCt                                               <  2:661937/141‑1 (MQ=255)
                                                                                     aagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCt                                               <  1:450946/141‑1 (MQ=255)
                                                                                      agaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCtt                                              <  1:396195/141‑1 (MQ=255)
                                                                                       gaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTg                                             <  2:596407/141‑1 (MQ=255)
                                                                                       gaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTg                                             <  1:441396/141‑1 (MQ=255)
                                                                                        aTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTgg                                            <  3:418150/141‑1 (MQ=255)
                                                                                         tCATGGTGATCAATTAATGCAATTGGCATTTGATAACCATTCGAAATATAACGTTACTTTCATTCTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGGTATAAAATTGTAGATAAGCTTggc                                           >  4:378379/1‑141 (MQ=255)
                                                                                         tCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGAAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggc                                           >  2:134863/1‑141 (MQ=255)
                                                                                           aTGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggcgg                                         >  2:561167/1‑141 (MQ=255)
                                                                                           aTGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggcgg                                         <  1:215439/141‑1 (MQ=255)
                                                                                             ggTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGgcggcg                                       <  3:419092/141‑1 (MQ=255)
                                                                                                     aTTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATg                               >  1:878799/1‑141 (MQ=255)
                                                                                                     aTTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATg                               >  1:878952/1‑141 (MQ=255)
                                                                                                      ttAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGa                              >  1:1008313/1‑141 (MQ=255)
                                                                                                       tAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAg                             <  1:274660/141‑1 (MQ=255)
                                                                                                       tAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAg                             >  1:518757/1‑141 (MQ=255)
                                                                                                        aaTGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGt                            <  2:260200/141‑1 (MQ=255)
                                                                                                          tGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTAc                          <  2:727396/141‑1 (MQ=255)
                                                                                                          tGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTAc                          >  3:351119/1‑141 (MQ=255)
                                                                                                            cAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCg                        <  2:236730/141‑1 (MQ=255)
                                                                                                             aaTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGt                       <  2:344725/141‑1 (MQ=255)
                                                                                                             aaTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGt                       >  1:108099/1‑141 (MQ=255)
                                                                                                              aTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                      >  2:964619/1‑141 (MQ=255)
                                                                                                              aTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                      <  1:989732/141‑1 (MQ=255)
                                                                                                              aTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                      <  2:144260/141‑1 (MQ=255)
                                                                                                                   cATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATTTGTTGTAAAATAATAAATAAACGATATAAAAGTTTAGATAAGCTGTGCGGCGGTGGCATGAGTACCGTTTATCt                 >  1:517202/1‑141 (MQ=255)
                                                                                                                   cATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCt                 >  1:412768/1‑141 (MQ=255)
                                                                                                                    aTTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCtt                <  2:1014584/141‑1 (MQ=255)
                                                                                                                      tAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGTTGATAAAGTATGATAAGTAAAATAATACATGAACGATATAAAATCGTAGATAAGCTTGGAGAAGGTAAAATGAGTAACGTCTATCTTGc              >  4:194057/1‑141 (MQ=255)
                                                                                                                       aGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGATATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAATGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCt             <  4:438051/141‑1 (MQ=255)
                                                                                                                           aaCCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAg         >  1:578341/1‑141 (MQ=255)
                                                                                                                           aaCCATACGAAATATAACGTTACTTTCATACTCGCGGCAATTGAAGGAGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAg         >  2:853003/1‑141 (MQ=255)
                                                                                                                             ccATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgat       <  4:339593/141‑1 (MQ=255)
                                                                                                                             ccATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgat       >  1:365918/1‑141 (MQ=255)
                                                                                                                             ccATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGCTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTAGCATGAGTACCGTTTATCTTGCTGAAgat       >  4:412431/1‑141 (MQ=255)
                                                                                                                                ttCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATaa                                                  >  4:144911/1‑95 (MQ=255)
                                                                                                                                ttCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATaa                                                  <  3:144911/95‑1 (MQ=255)
                                                                                                                                ttCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacg    <  3:26883/141‑1 (MQ=255)
                                                                                                                                 tCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga   <  2:145696/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGAT  >  USA300TCH1516_ALE/1231917‑1232187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: