Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	322.1 / NA	89	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (47/42);  total (47/42)

TCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACG  >  USA300TCH1516_ALE/1646057‑1646325                                                                                                                                      |                                                                                                                                         tCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTaca                                                                                                                                  >  1:482856/1‑141 (MQ=255)  cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                                 <  4:402234/141‑1 (MQ=255)  cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                                 >  1:597812/1‑141 (MQ=255)        cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTa                                                                                                                           <  2:8724/141‑1 (MQ=255)          acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                         >  2:971268/1‑141 (MQ=255)              cATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                                                                     >  3:386097/1‑141 (MQ=255)                tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                   >  2:672702/1‑141 (MQ=255)                  tgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                                 <  1:714623/141‑1 (MQ=255)                     tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                              <  4:39055/141‑1 (MQ=255)                       aTGCACCTCTCTGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATg                                                                                                            <  2:795730/141‑1 (MQ=255)                       aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATg                                                                                                            >  1:723638/1‑141 (MQ=255)                           aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtc                                                                                                        <  1:345810/141‑1 (MQ=255)                           aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCCAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATGCATATGTTtc                                                                                                        <  1:783113/141‑1 (MQ=255)                              gatATGAGCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                                                                     <  4:239364/139‑1 (MQ=255)                               ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                    >  4:96982/1‑141 (MQ=255)                                     ccAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAata                                                                                              >  1:786808/1‑141 (MQ=255)                                        gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTa                                                                                           <  1:516559/141‑1 (MQ=255)                                         aTGCTACAATCGTATCGAATGATGGTCCTTCTGCTCCTTGTGGTTGCAGAGGGGGTGCTAACATCCCCTGTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                                                                          <  1:612928/141‑1 (MQ=255)                                             tACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                                                                      <  4:256378/141‑1 (MQ=255)                                              aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                     >  2:1025318/1‑141 (MQ=255)                                                aaTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttt                                                                                   <  2:501441/141‑1 (MQ=255)                                                aaTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttt                                                                                   >  1:560397/1‑141 (MQ=255)                                                aaTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttt                                                                                   <  1:622761/141‑1 (MQ=255)                                                 aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                  <  1:268619/141‑1 (MQ=255)                                                 aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                  <  4:226819/141‑1 (MQ=255)                                                     tATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttaattc                                                                              <  1:959677/141‑6 (MQ=255)                                                      aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACCACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                             <  1:523811/141‑1 (MQ=255)                                                      aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                                                                          <  4:406509/128‑1 (MQ=255)                                                      aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                                                                          >  3:406509/1‑128 (MQ=255)                                                       tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                            <  3:205270/141‑1 (MQ=255)                                                        cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTa                                                                           >  3:187036/1‑141 (MQ=255)                                                           atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                        >  1:944458/1‑141 (MQ=255)                                                           atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                        <  2:1005739/141‑1 (MQ=255)                                                             gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                                      <  2:649855/141‑1 (MQ=255)                                                               tgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                                    >  4:111140/1‑141 (MQ=255)                                                                ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                   <  2:482856/141‑1 (MQ=255)                                                                  tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                 >  1:128706/1‑141 (MQ=255)                                                                  tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                 >  2:553225/1‑141 (MQ=255)                                                                  tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                 >  1:981537/1‑141 (MQ=255)                                                                   ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAATAGTTTACATATATTCATATGTTTGATCAACATTATTAGTTGCTTTTTGTATTAAAGCATTTTCg                                                                >  4:128304/1‑141 (MQ=255)                                                                        tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc                                                           >  2:885296/1‑141 (MQ=255)                                                                          cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTAATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                         >  1:593742/1‑141 (MQ=255)                                                                           tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                                                                          >  1:49920/1‑91 (MQ=255)                                                                           tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                                                                          <  2:49920/91‑1 (MQ=255)                                                                                  tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACg                                                 <  2:597812/141‑1 (MQ=255)                                                                                   cTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                                >  1:79911/1‑141 (MQ=255)                                                                                    tAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                               >  2:868492/1‑141 (MQ=255)                                                                                    tAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                               >  3:196267/1‑141 (MQ=255)                                                                                     aGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTAATTAAATCAATTTCGTCAGCATCTTTGACGtct                                              >  2:294700/1‑141 (MQ=255)                                                                                     aGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                              <  1:274788/141‑1 (MQ=255)                                                                                     aGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                              >  2:500858/1‑141 (MQ=255)                                                                                      gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                             >  1:846456/1‑141 (MQ=255)                                                                                      gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                             >  4:213368/1‑141 (MQ=255)                                                                                      gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                             <  2:911761/141‑1 (MQ=255)                                                                                      gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                             >  1:824942/1‑141 (MQ=255)                                                                                       cATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                            <  2:776571/141‑1 (MQ=255)                                                                                       cATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                            >  2:927500/1‑141 (MQ=255)                                                                                         ttttGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                          >  1:68047/1‑141 (MQ=255)                                                                                            tGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCGTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                       <  3:368253/141‑1 (MQ=255)                                                                                             gCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                      >  2:945810/1‑141 (MQ=255)                                                                                              cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATAAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                     <  2:781771/141‑1 (MQ=255)                                                                                                       atTGCCTTTAATTCTTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGt                             <  3:77369/141‑1 (MQ=255)                                                                                                        ttGCCTTTAATTC‑TTTTTTTTTTTTTTCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCTTCAACAATATTAGCTGCTTTTTTCTTTATTTCAATTTTGTTAGCATCTTTTATTTTTCTATTTTTATCTATTGTAt                           >  2:810132/1‑141 (MQ=255)                                                                                                        ttGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAt                           >  2:135412/1‑141 (MQ=255)                                                                                                        ttGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAt                           <  2:404168/141‑1 (MQ=255)                                                                                                         tGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                          >  2:701838/1‑141 (MQ=255)                                                                                                          gCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGCCGTCTCTAATTTTATCTACAGTATTa                         >  2:537129/1‑141 (MQ=255)                                                                                                          gCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                         >  3:139575/1‑141 (MQ=255)                                                                                                           ccTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                      >  1:652589/1‑47 (MQ=255)                                                                                                           ccTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                      >  1:652322/1‑47 (MQ=255)                                                                                                           ccTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                      <  2:652322/47‑1 (MQ=255)                                                                                                           ccTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                      <  2:652589/47‑1 (MQ=255)                                                                                                            cTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                       <  2:46885/141‑1 (MQ=255)                                                                                                                aaTTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                   <  1:874049/141‑1 (MQ=255)                                                                                                                aaTTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                   <  1:874036/141‑1 (MQ=255)                                                                                                                 aTTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                  <  2:766618/141‑1 (MQ=255)                                                                                                                  ttC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                 >  1:826724/1‑141 (MQ=255)                                                                                                                   ttatttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAACTGCt                 <  4:205762/138‑1 (MQ=255)                                                                                                                    c‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta               >  2:69269/1‑141 (MQ=255)                                                                                                                      tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat              >  1:68884/1‑141 (MQ=255)                                                                                                                       ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt             >  1:291780/1‑141 (MQ=255)                                                                                                                           cAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg         >  4:15443/1‑141 (MQ=255)                                                                                                                            aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa        >  1:917386/1‑141 (MQ=255)                                                                                                                             gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat       >  1:453091/1‑141 (MQ=255)                                                                                                                               cATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat     >  1:45522/1‑141 (MQ=255)                                                                                                                                aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAtata    <  2:707773/141‑1 (MQ=255)                                                                                                                                  gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg  <  2:1025820/141‑1 (MQ=255)                                                                                                                                  gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg  <  2:769586/141‑1 (MQ=255)                                                                                                                                  gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg  <  2:534458/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACG  >  USA300TCH1516_ALE/1646057‑1646325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑