| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,679,803 | G→T | G96V (GGA→GTA) | ssaA2_4 → | Staphylococcal secretory antigen ssaA2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,679,803 | 0 | G | T | 98.2% | 378.5 / ‑2.0 | 112 | G96V (GGA→GTA) | ssaA2_4 | Staphylococcal secretory antigen ssaA2 |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (50/60); total (51/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
AAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACT > USA300TCH1516_ALE/2679667‑2679937 | aaaCGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTatat > 1:706119/1‑141 (MQ=255) aaCGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTAtatt > 2:824430/1‑141 (MQ=255) aaCGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTAtatt > 1:437077/1‑141 (MQ=255) cGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTAtattat > 4:48865/1‑141 (MQ=255) gTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATg < 2:114175/141‑1 (MQ=255) cgcACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACa < 4:300334/141‑1 (MQ=255) gcACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACAt > 3:420858/1‑141 (MQ=255) gcACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACAt > 2:639467/1‑141 (MQ=255) cacaAGTCGGTAAACCTGTGTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACAGCAGTATATTATGGACATg > 2:111813/1‑141 (MQ=255) caAGTCGGTAAACCTGTTTCTAAGAGATGGGGAAATGCTAAAAAGTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAAGCAACTGCAGTATATTATGGACATGTc < 1:618383/141‑1 (MQ=255) aGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGc < 3:86976/141‑1 (MQ=255) gTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCa > 4:120829/1‑141 (MQ=255) ggTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgtt < 3:147117/141‑1 (MQ=255) gTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttg > 4:58024/1‑141 (MQ=255) gTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttg > 2:270195/1‑141 (MQ=255) tAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttgt < 1:864520/141‑1 (MQ=255) aaCCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGttgttg > 1:414626/1‑141 (MQ=255) ccTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGaa > 1:513421/1‑141 (MQ=255) ccTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGaa < 2:437077/141‑1 (MQ=255) ccTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGaa > 1:979596/1‑141 (MQ=255) gTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACaa > 3:9209/1‑141 (MQ=255) ttCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGt < 1:505954/141‑1 (MQ=255) cTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGtat < 3:120829/141‑1 (MQ=255) agagagGGGGGAGTGCTAAGAAGTGGGAGTACGATGCAGGTAGATCAAAATATGCGACTGGTCGTACACCAAGGAAATGTGGTGGCATGCAATCAACTGCAGTATATGGTGGACATGTCGCAGTTGTTGAACAAGtatata < 4:399796/139‑1 (MQ=255) aTAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGtatata < 1:564812/141‑1 (MQ=255) aTGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATaaaaa > 1:703856/1‑141 (MQ=255) ggggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACg > 1:371930/1‑141 (MQ=255) gggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg > 3:358015/1‑141 (MQ=255) gggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg < 3:160814/141‑1 (MQ=255) gggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg < 2:399946/141‑1 (MQ=255) gggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg < 2:399919/141‑1 (MQ=255) ggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgt > 1:985870/1‑141 (MQ=255) gCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgta < 4:247861/141‑1 (MQ=255) aTGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgtagta > 2:983723/1‑141 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 1:54933/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 2:717757/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 3:194781/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 2:74410/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 3:162889/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 1:621816/141‑1 (MQ=255) cTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTa < 1:612080/141‑1 (MQ=255) aaaaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttgt > 1:889880/1‑110 (MQ=255) aaaaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttgt < 2:889880/110‑1 (MQ=255) aaaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGGCATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAg < 2:931037/141‑1 (MQ=255) aaaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAg < 3:244768/141‑1 (MQ=255) aaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGt > 1:707919/1‑141 (MQ=255) aaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGt < 1:314493/141‑1 (MQ=255) ggTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAg < 3:330117/141‑1 (MQ=255) gTATTNCAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTGATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGa < 3:118460/141‑1 (MQ=255) aCAATGCACGTAAATCAAAATATGCGACTGGTCGTACACTAAGAAAAATTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTCTCTACAAACGGTAATATTAAAGTATCCGCATACa > 4:342609/1‑141 (MQ=255) cAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACaa < 1:474351/141‑1 (MQ=255) cAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACaa > 1:466241/1‑141 (MQ=255) aaTGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAAc < 1:788178/141‑1 (MQ=255) tGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGtatat > 1:273750/1‑106 (MQ=255) tGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGtatat < 2:273750/106‑1 (MQ=255) tGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACtt > 4:174011/1‑141 (MQ=255) gCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACttt > 3:295894/1‑141 (MQ=255) aCGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTa > 2:49851/1‑141 (MQ=255) cGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTAt > 4:224425/1‑141 (MQ=255) tAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCg < 2:643269/141‑1 (MQ=255) tAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCg < 1:767660/141‑1 (MQ=255) aaaTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGc < 2:371930/141‑1 (MQ=255) aaaTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCGGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGc > 1:803453/1‑141 (MQ=255) aaTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGcc > 2:962066/1‑141 (MQ=255) aTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGccc > 1:513533/1‑141 (MQ=255) aTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGccc > 3:407632/1‑141 (MQ=255) aTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGccc < 2:243476/141‑1 (MQ=255) tCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCa < 1:177566/141‑1 (MQ=255) cAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCAt < 1:991745/141‑1 (MQ=255) cAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCAt < 4:257407/141‑1 (MQ=255) cAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCAt < 3:305385/141‑1 (MQ=255) aaTATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTaa < 2:92515/141‑1 (MQ=255) gcgcCTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAaatac < 1:445030/141‑1 (MQ=255) gCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAaatac < 2:941380/141‑1 (MQ=255) acCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACt > 1:316220/1‑141 (MQ=255) cAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAGTACAATACACGTGTACTaa > 2:433845/1‑141 (MQ=255) cAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTaa > 3:40685/1‑141 (MQ=255) cAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTAAGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGAAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTaa > 4:59768/1‑141 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAGGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCaaaaa > 1:471061/1‑141 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGcc < 1:13385/107‑1 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGcc > 2:13385/1‑107 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCaaaaa > 3:171508/1‑141 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCaaaaa > 2:905839/1‑141 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGAACTAAGCaaaaa > 2:184750/1‑141 (MQ=255) ttGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAgg > 2:189426/1‑141 (MQ=255) tGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgc > 1:1025349/1‑141 (MQ=255) tGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgc > 2:942538/1‑141 (MQ=255) cTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACTGTAGTATTAAAGTTTCAGAATACAACTTTTGTCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgcag > 1:617088/1‑141 (MQ=255) cTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgcag > 3:66766/1‑141 (MQ=255) tGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgcagc < 1:527737/141‑1 (MQ=255) gTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgcagca < 3:305591/141‑1 (MQ=255) aTGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGt < 2:755538/141‑1 (MQ=255) aTGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGt < 1:321783/141‑1 (MQ=255) aaTCAACNGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACt < 2:771557/141‑1 (MQ=255) tCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACttt < 1:707136/141‑1 (MQ=255) tCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACttt < 4:295894/141‑1 (MQ=255) aCTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAAc < 1:933509/141‑1 (MQ=255) aCTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAAc > 1:567348/1‑141 (MQ=255) aCTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAACAAAAAGGCAGCACGTAACTTTAAc < 2:129834/141‑1 (MQ=255) cTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACt > 4:267628/1‑141 (MQ=255) | AAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACT > USA300TCH1516_ALE/2679667‑2679937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |