BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,455,692	Δ1 bp	100%	coding (1210/1260 nt)	lyrA →	Lysostaphin resistance protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,455,692	0	G	.	88.5%	388.2 / 35.3	104	coding (1210/1260 nt)	lyrA	Lysostaphin resistance protein A
Reads supporting (aligned to +/- strand): ref base G (2/1); major base . (42/50); minor base A (6/3); total (50/54)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 110 total aligned reads displayed.

CAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT  >  USA300TCH1516_ALE/2455558‑2455825

cagcagAAGCAGTTAACCAAGCACCACAACGTGACGATTTAACAAACGATTCAAAGGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaat                                                                                                                                   <  1:15613/141‑2 (MQ=255)

  gcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta                                                                                                                                 <  1:787006/141‑4 (MQ=255)

  gcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCAGCGACGTATAAAGACGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta                                                                                                                                 <  1:128731/141‑4 (MQ=255)

  gcagAATCAGATAAACAAGCACCACAAAGTGACGAATTAACAAACGATTCAAAGGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta                                                                                                                                 <  1:491800/141‑4 (MQ=255)

    agAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatc                                                                                                                               >  1:720161/1‑136 (MQ=255)

    agAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatc                                                                                                                               >  2:506130/1‑136 (MQ=255)

          aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑Gaaaaa                                                                                                                       >  1:326947/1‑141 (MQ=255)

                aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGCGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTgaa                                                                                                                >  1:319968/1‑141 (MQ=255)

                 cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTgaag                                                                                                               <  1:51484/141‑1 (MQ=255)

                 cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTgaag                                                                                                               <  1:704739/141‑1 (MQ=255)

                 cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATC‑GAAAAA‑ACTgaag                                                                                                               >  1:613118/1‑141 (MQ=255)

                  aaGCACCACAAAGTGACGATTAAAAAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAATATA‑GAAAAA‑ACTgaaga                                                                                                              <  2:10007/141‑1 (MQ=255)

                    gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTgaagaaa                                                                                                            >  2:349835/1‑140 (MQ=255)

                    gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAAc                                                                                                            >  1:713806/1‑141 (MQ=255)

                    gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAAc                                                                                                            <  1:843822/141‑1 (MQ=255)

                     caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAGAAATTATC‑GAAAAA‑ACTGAAGAACa                                                                                                           >  2:604766/1‑141 (MQ=255)

                     caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCACTAAAAGCACCTGCGACGTAGAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACa                                                                                                           >  1:180670/1‑141 (MQ=255)

                         acaAAGTGACGATTTAACAAACTATTCAAATGAAGATAAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCt                                                                                                       <  1:176841/141‑1 (MQ=255)

                         acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAA‑ATTTCAGAAAAA‑ACTGAAGGACAATCt                                                                                                       >  1:18077/1‑141 (MQ=255)

                            aaGTGACGATTAAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCa                                                                                                    <  2:159905/141‑1 (MQ=255)

                             attGACGAGCTAACAAACGGTGCAGATGAAAATGAAATTGAGCAATGATTAAAAGAACCTGCGACTTATAAAGAATACAGACTTTCACCAGTTTTAATTGATGCA‑AAAAAATGATC‑GAAAAA‑ACTGAAGAACAATCTTCAg                                                                                                   <  2:25743/139‑1 (MQ=255)

                             aGTGACGAATTAACAAAAGATTAAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGAAAAAAAAAAA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAg                                                                                                   <  2:462890/141‑1 (MQ=255)

                              gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGa                                                                                                  <  1:489446/141‑1 (MQ=255)

                               tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGAt                                                                                                 <  1:346334/141‑1 (MQ=255)

                                caCGATTAAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATa                                                                                                <  1:890175/140‑1 (MQ=255)

                                  cGATTTAACAAACGATTGAAGTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATaaa                                                                                              <  2:825574/141‑1 (MQ=255)

                                       tAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTAAGATAAAAACaa                                                                                         >  2:437739/1‑141 (MQ=255)

                                            aaCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAaataat                                                                                    >  2:708698/1‑141 (MQ=255)

                                             aCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAataata                                                                                   <  2:669186/141‑1 (MQ=255)

                                               gATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACATATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc                                                                                 <  2:897316/141‑1 (MQ=255)

                                                aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa                                                                                <  1:332517/141‑1 (MQ=255)

                                                 ttCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                               <  1:501733/141‑1 (MQ=255)

                                                 atgaaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                               <  2:785216/138‑1 (MQ=255)

                                                    aaaaGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc                                                                            <  1:723532/141‑1 (MQ=255)

                                                    aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATAAGTTGTTATTGATGCA‑AAACATCT‑TC‑GAAAAATACTGAAGACAAATCTTGCGGGTAAAACAAATAATAAAAAAgc                                                                            >  1:658115/1‑141 (MQ=255)

                                                     aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACACAgcg                                                                           >  2:394998/1‑141 (MQ=255)

                                                     aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTAGAAAGAATACAGACGTTCATCATTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACGGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                           <  2:444748/141‑1 (MQ=255)

                                                            aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTa                                                                    <  1:421932/141‑1 (MQ=255)

                                                             tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                   >  1:279364/1‑141 (MQ=255)

                                                                aaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGCTCATCAGTTGTAATTGATGCAAAAAAA‑TTATC‑GAAAAA‑ACTGAAGAACCATCTCCAGATAAGAACAAATAATAACAAAGCGCTAGCTAGCAc                                                                >  1:111734/1‑141 (MQ=255)

                                                                 aTAGAGCAATCATTCAAAGCACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc                                                               >  1:499868/1‑141 (MQ=255)

                                                                 aTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc                                                               >  1:423226/1‑141 (MQ=255)

                                                                   agagCAAACATTAAAAGAACCTACGAGTTATACAGAAGACAGACGTTCATGAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAGCAAAGCGCTTGCTAGTACctc                                                             <  2:865797/141‑1 (MQ=255)

                                                                    agggaaacATTAAAAAAACATACGACTTATAAAGAAGACAGACGTTCATTAGTTGTAATTGATGCAAAAAAATA‑ATA‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                            <  2:6960/134‑1 (MQ=255)

                                                                     agCAATCATTAAAAGAACCTGCGACTTGTAAACAAAACAGACGTGCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTAAGATAAAAAAAAATAAAAACAAAGCGCTTGCTAGTACCTCtt                                                           >  2:52352/1‑141 (MQ=255)

                                                                     agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt                                                           >  2:782480/1‑141 (MQ=255)

                                                                     agCAATCATTAAAAGAACCGGCGACTTATAAAGAGGACAGACGTTCATTAGTTGTAATTGAGGC‑GAAGAAATAAGC‑GAAAAA‑AGTGAAGAGCAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCGAGTACCTCtt                                                           >  1:680993/1‑141 (MQ=255)

                                                                        aaacATTAAAAGAACCTGCGACTTATAAAGAAAACAGCCATTAATACATAGTCATTGGTAC‑GATAAAACTATC‑GAAAAA‑ATCGAAGTACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  2:191623/138‑1 (MQ=255)

                                                                        aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  1:513771/141‑1 (MQ=255)

                                                                        aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  2:302616/141‑1 (MQ=255)

                                                                         aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                       <  1:166715/141‑1 (MQ=255)

                                                                         aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAGTAATGGA‑ACAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTGCAGATAAAAACAAATAATAACAAAGCGCTTGATATGACCTCTTaaga                                                       >  1:253965/1‑139 (MQ=255)

                                                                          tCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa                                                      >  1:817113/1‑141 (MQ=255)

                                                                           cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                     <  2:14945/141‑1 (MQ=255)

                                                                            aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAga                                                    >  2:168243/1‑141 (MQ=255)

                                                                             ttAAGAGAACCTGCGACTTATAAAGAAGACCGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGATTGCTAGTAACTCTTAAAAAgac                                                   >  2:554784/1‑140 (MQ=255)

                                                                             ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAGCAAACTTCAGATAAAAACGAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat                                                   <  2:818051/141‑1 (MQ=255)

                                                                               aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGC‑GAAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTACAAAgatga                                                 >  1:395517/1‑141 (MQ=255)

                                                                                 aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATGGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                               <  2:180670/141‑1 (MQ=255)

                                                                                   gAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                             <  1:379274/141‑1 (MQ=255)

                                                                                    aaCCTACAACATATAAAGAAGACAGACGTTCATCAGTAGAAAAAAAAAAA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTa                                            <  2:609547/141‑1 (MQ=255)

                                                                                     aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAGGCTGCTAg                                           >  2:859343/1‑141 (MQ=255)

                                                                                      ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTGGc                                          >  2:749491/1‑141 (MQ=255)

                                                                                      ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc                                          >  2:742796/1‑141 (MQ=255)

                                                                                        tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                        <  2:644744/141‑1 (MQ=255)

                                                                                          cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      <  1:273517/141‑1 (MQ=255)

                                                                                          cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      >  1:648315/1‑141 (MQ=255)

                                                                                          cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      >  1:712516/1‑141 (MQ=255)

                                                                                          cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      >  1:845056/1‑141 (MQ=255)

                                                                                            aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGCTATTAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                    <  1:394998/141‑1 (MQ=255)

                                                                                            aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                    >  2:701018/1‑141 (MQ=255)

                                                                                            aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                    >  2:815026/1‑141 (MQ=255)

                                                                                             cTTCTAAAGAAGACAGACGTTCAGCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                   <  1:764243/141‑1 (MQ=255)

                                                                                                ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                <  1:752178/141‑1 (MQ=255)

                                                                                                ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                <  1:741196/141‑1 (MQ=255)

                                                                                                ataAAGAAGACAGACGTTCATCAGTTGTAATAGATACA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAGCGCtttt                                <  2:221500/141‑1 (MQ=255)

                                                                                                 taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                               >  2:168651/1‑141 (MQ=255)

                                                                                                 taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                               >  1:455535/1‑141 (MQ=255)

                                                                                                 aaaaGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAACAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                               <  2:722937/140‑1 (MQ=255)

                                                                                                    agaagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCTAGCGCGTTTctat                            >  1:286962/1‑141 (MQ=255)

                                                                                                     gaagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctata                           >  2:803081/1‑141 (MQ=255)

                                                                                                      aagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATGTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTATCAGGGGCttttttctat                          >  2:78996/1‑135 (MQ=255)

                                                                                                      aagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTCAAAAGATGATGCTAGCAAGCGCGTTTctatac                          >  2:629861/1‑141 (MQ=255)

                                                                                                           agagGTTCATCACTTGTAATTCATGCA‑AAAAAATTATC‑AAAAAA‑ACTTAATAACAATCTTCAGATAAAAACAAATAACAACAAAGCGCTTGCTAGTACCTCTTAAATAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     <  2:387410/141‑1 (MQ=255)

                                                                                                           agacGTTTATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACAGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     <  1:2522/141‑1 (MQ=255)

                                                                                                           agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     >  2:212396/1‑141 (MQ=255)

                                                                                                           agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     <  2:515001/141‑1 (MQ=255)

                                                                                                            gacGTTCATCAGTTGTGAGTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAGCGCTTTTCTATACtatata                    <  2:365630/141‑1 (MQ=255)

                                                                                                            gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACTAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatatt                    >  2:653355/1‑140 (MQ=255)

                                                                                                            gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAGCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACcatata                    >  2:467448/1‑141 (MQ=255)

                                                                                                            gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCACGCGCTTTTCTATACtatata                    >  2:520137/1‑141 (MQ=255)

                                                                                                              cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTGTC‑GAAAAA‑ACTGACGAACAGTCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTGCCGGTGAAAAAGCAGATGCTAGCAGCCCCTCTTgtatacaatggatt                  >  2:475553/1‑136 (MQ=255)

                                                                                                              cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTTTATACTATAtttt                  >  1:417650/1‑141 (MQ=255)

                                                                                                              cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTAAAtatt                  >  2:161574/1‑141 (MQ=255)

                                                                                                                 tcatcaGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt               <  2:606687/141‑1 (MQ=255)

                                                                                                                   atcaGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt             <  1:708698/141‑1 (MQ=255)

                                                                                                                     caGTTGTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc           <  1:834976/141‑1 (MQ=255)

                                                                                                                      aGTTGTAATTGATGCAAAAAAA‑TTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCCCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTCTATATTATTTTTCt          >  2:516928/1‑141 (MQ=255)

                                                                                                                          gTAATTGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaaa      <  2:720161/141‑1 (MQ=255)

                                                                                                                              ttGATGCA‑AAAAAATTATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt  <  1:152395/141‑1 (MQ=255)

CAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATC‑GAAAAA‑ACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT  >  USA300TCH1516_ALE/2455558‑2455825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`