BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000731	25,663	Δ1 bp	100%	coding (316/333 nt)	USA300HOU_pUSA300HOUMR0031 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	25,663	0	A	.	95.9%	1196.9 / 27.3	269	coding (316/333 nt)	USA300HOU_pUSA300HOUMR0031	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/9); new base . (142/116); total (143/126)
Fisher's exact test for biased strand distribution p-value = 6.93e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 285 total aligned reads displayed.

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAAT  >  CP000731/25523‑25799

attgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCGCCAAGTATTAAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACCAATa                                                                                                                                          >  2:193368/1‑141 (MQ=255)

 ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAATaa                                                                                                                                         <  1:245786/141‑1 (MQ=255)

       gTTATTTACACTAGTTCTTAAATTTACAGGTTTTTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAATaaaaaata                                                                                                                                   <  2:448192/141‑3 (MQ=255)

         tATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAATaaaaaatata                                                                                                                                 <  2:715230/141‑5 (MQ=255)

          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATAc                                                                                                                               >  1:818562/1‑141 (MQ=255)

          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATAc                                                                                                                               >  2:310884/1‑141 (MQ=255)

           tttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTCATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACa                                                                                                                              >  1:851312/1‑141 (MQ=255)

            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACAt                                                                                                                             <  1:152449/141‑1 (MQ=255)

              acacTAGTGCTTAAATTTACAGGTTAAACACCAAGTAGTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATAATAAAAAT‑AAAAAATATACATCt                                                                                                                           <  2:916821/141‑1 (MQ=255)

                acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTaa                                                                                                                         <  1:596678/141‑1 (MQ=255)

                 cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTaaa                                                                                                                        >  1:363658/1‑141 (MQ=255)

                 cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTaaa                                                                                                                        >  1:304806/1‑141 (MQ=255)

                  tAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAg                                                                                                                       <  1:316184/141‑1 (MQ=255)

                   aGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAgg                                                                                                                      >  1:415008/1‑141 (MQ=255)

                       cTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaa                                                                                                                  >  1:894795/1‑141 (MQ=255)

                       cTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaa                                                                                                                  >  1:857820/1‑141 (MQ=255)

                        ttAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaaa                                                                                                                 >  2:171011/1‑141 (MQ=255)

                        ttAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaaa                                                                                                                 <  1:385896/141‑1 (MQ=255)

                        ttAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTATTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaaa                                                                                                                 >  2:581826/1‑141 (MQ=255)

                        ttAAATTGACAAGATACTCACTAAGTATTGTATGATTCTGGTTTTCTATATAACCAGAATTTTCCTTCAGAATTGTGGTTATAGCTCTTATG‑TTTTTATTTAGCTATA‑AAAAAAAAAAAAAATATACATCTAAAGGTTaaa                                                                                                                 <  2:79357/141‑1 (MQ=255)

                          aaaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAtt                                                                                                               <  1:325591/141‑1 (MQ=255)

                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTa                                                                                                              <  2:213090/141‑1 (MQ=255)

                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTa                                                                                                              >  1:758065/1‑141 (MQ=255)

                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTATTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaaaaaa                                                                                                              <  2:473882/141‑4 (MQ=255)

                                aCAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCACAATATCCAGAAATTTTCTTTATAAATGTAGAACTATTTATTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaa                                                                                                         <  1:61700/141‑1 (MQ=255)

                                 cAGGTTTGTCATCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTCCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        <  2:260071/141‑1 (MQ=255)

                                 cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        <  1:560784/141‑1 (MQ=255)

                                 cAGGTTTGTCAACAAGTATTGAAGGATCTTGGTTTTTTCAATTGCCGTGTGGTTGTTTTGGAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATAATACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        <  2:320083/141‑1 (MQ=255)

                                     tttGTTACCAAGTATTGAAGGATTTTGGTTTTCTCAATATTCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATa                                                                                                    <  1:208289/141‑1 (MQ=255)

                                     tttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACCATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATa                                                                                                    <  1:271590/141‑1 (MQ=255)

                                      ttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATAAAGAATAg                                                                                                   >  1:384243/1‑141 (MQ=255)

                                       tGTCACCAAGTATTGAATGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGa                                                                                                  <  2:609703/141‑1 (MQ=255)

                                       tGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGa                                                                                                  >  2:526055/1‑141 (MQ=255)

                                          cACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGACtt                                                                                               >  1:114480/1‑141 (MQ=255)

                                          aacaaaGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTAAAATACAAA‑AAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAtt                                                                                               <  2:812762/137‑1 (MQ=255)

                                          aaCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAtt                                                                                               <  2:492975/140‑1 (MQ=255)

                                           aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                              <  1:734954/141‑1 (MQ=255)

                                             cAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTCTTACTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAGTAGAATTagg                                                                                            >  1:123427/1‑140 (MQ=255)

                                             cAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACACCTAAAGGTTAAATTAATGAAAAATAGAATTaaa                                                                                            >  1:77151/1‑139 (MQ=255)

                                             cAAGTATTAAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGGTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAAGTTAAATTAATTAAAAATAGacatata                                                                                            >  2:678100/1‑135 (MQ=255)

                                              aaGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTagag                                                                                           <  2:807899/141‑1 (MQ=255)

                                              aaGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTagag                                                                                           <  1:491456/141‑1 (MQ=255)

                                                gTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGaa                                                                                         <  1:524198/141‑1 (MQ=255)

                                                gTATTGAAGGAACTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGaa                                                                                         <  1:141661/141‑1 (MQ=255)

                                                    tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                     >  2:440572/1‑141 (MQ=255)

                                                       agtagcTTGGTTTTCCTAATATCCAAAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                  <  2:221146/136‑1 (MQ=255)

                                                       aGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                  >  2:870993/1‑141 (MQ=255)

                                                          aTCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAc                                                                               >  1:78706/1‑141 (MQ=255)

                                                            cTTGGTTTTCTCAATATCCAGCAGTGTGCATCTTAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaa                                                                             >  1:218270/1‑141 (MQ=255)

                                                             ttagTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGAAATATTTATTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaa                                                                            <  2:181957/138‑1 (MQ=255)

                                                               ggTTTTCTCAATATCCCGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                          >  2:762311/1‑141 (MQ=255)

                                                                tttttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                         <  1:585708/140‑1 (MQ=255)

                                                                gTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                         >  1:279702/1‑141 (MQ=255)

                                                                  tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAAGTTAAATTAATGAAAAATAGAATTAAAGAATATAGAAAAAAAAAAGGa                                                                       >  2:592649/1‑141 (MQ=255)

                                                                     ctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAtt                                                                    >  2:105786/1‑141 (MQ=255)

                                                                       cAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtc                                                                  >  2:293469/1‑141 (MQ=255)

                                                                        aaTATCCAGAAATTTTCTTTATAATTGTAGCAATATGTCTTATGCTGTTTAATTA‑CTAAACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtct                                                                 <  2:362743/141‑1 (MQ=255)

                                                                        aaTATCCAGAAATTTTCTTAAAAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAGATAGAAAACAAAAAGGAATTtct                                                                 <  1:446558/141‑1 (MQ=255)

                                                                            ttcAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                             <  2:537062/139‑1 (MQ=255)

                                                                            tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                             <  2:152156/141‑1 (MQ=255)

                                                                            accAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAATATAAAGGCGATAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                             <  1:902761/140‑1 (MQ=255)

                                                                              cAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGaa                                                           >  1:482630/1‑141 (MQ=255)

                                                                                   aTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACttt                                                      >  2:133850/1‑141 (MQ=255)

                                                                                     tttCTTTATAATTGTCGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAGAAATAGAATAAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTCTaa                                                    >  2:425817/1‑141 (MQ=255)

                                                                                     tttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTaa                                                    >  2:889174/1‑141 (MQ=255)

                                                                                       tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                  >  1:193427/1‑141 (MQ=255)

                                                                                       tCTTTATAATTGTAGCACTATTTCTTATGCTGTGTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAATAGAGCATATAGGAAACAAAAAGGAATTTCTCAAGAAAATTTAAGt                                                  >  1:182900/1‑141 (MQ=255)

                                                                                        cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                 >  1:801885/1‑141 (MQ=255)

                                                                                         tttATAGTTGTAGAACTATTTCTTATGCTGTTTAATTA‑TTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATTGAATTAGAGAATAAAGAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTaa                                                <  2:190116/141‑1 (MQ=255)

                                                                                         tttATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaa                                                >  2:353654/1‑141 (MQ=255)

                                                                                          ttATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaaa                                               <  2:903480/141‑1 (MQ=255)

                                                                                          ttATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACAACTAAAAGTTAAAATAATAAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaaa                                               <  1:612430/141‑1 (MQ=255)

                                                                                           tataATTGTAGCACTAGTTCTCATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAACACACACAGGAATTTCACAAGAAACTTTAAGTAAAg                                              >  1:467302/1‑141 (MQ=255)

                                                                                              aaTTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAt                                           <  1:606451/141‑1 (MQ=255)

                                                                                               aTTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                          >  1:646673/1‑141 (MQ=255)

                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAATGAATTTCTCAAGAAACTTTAAGTAGAGAATTaaa                                       >  1:340057/1‑141 (MQ=255)

                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       >  2:822322/1‑141 (MQ=255)

                                                                                                    aGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAg                                     >  2:38601/1‑141 (MQ=255)

                                                                                                     gCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgt                                    >  1:664906/1‑141 (MQ=255)

                                                                                                       aCTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTCATGAAAAATAGAATTAGAGAATGTAGAAAACCAAAAGGAATTTCTCAAGAAACTTTCAGTAAAGAATTAAAAgtgt                                  >  2:501272/1‑141 (MQ=255)

                                                                                                       aCTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACACCCAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                  <  1:72339/141‑1 (MQ=255)

                                                                                                        cTATTTCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACACCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGACTAAAAAgtgaa                                 >  2:316224/1‑139 (MQ=255)

                                                                                                        cTATTTCTTATGCTGTTTAATTA‑CTATACTACAAA‑GAAAAAATATACACATAAATGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTATCAAGAAATTTTAAGTAAAGAATTAAAAGTGTc                                 <  1:298492/141‑1 (MQ=255)

                                                                                                         tATTTTGTATGCTGTTTAATTA‑CTATACTAAAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTGCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                                <  2:6713/141‑1 (MQ=255)

                                                                                                          aTTTTTTATGGTGTTTAATTA‑GTTGGGTAGAGAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAGATAGTATTAGAGAATAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTa                               <  2:637643/141‑1 (MQ=255)

                                                                                                           tttCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                              >  2:72935/1‑141 (MQ=255)

                                                                                                           tttCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                              <  1:690185/141‑1 (MQ=255)

                                                                                                           tttCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                              >  2:138630/1‑141 (MQ=255)

                                                                                                            ttCTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAgg                             <  1:78490/141‑1 (MQ=255)

                                                                                                              cTTATGCTGTTTAATTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                           >  2:278087/1‑141 (MQ=255)

                                                                                                                    ttttttAATTA‑CTATACTACAAA‑AAAAAAATATACATCTAAAGGTTAAATTAATGAAAAAGAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                     <  1:202419/138‑1 (MQ=255)

                                                                                                                           aTTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCa              >  2:50821/1‑141 (MQ=255)

                                                                                                                           aTTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCa              <  1:133850/141‑1 (MQ=255)

                                                                                                                           aTTA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGACTAGGCAAACTATTAATGCa              >  2:693411/1‑141 (MQ=255)

                                                                                                                            ttA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTTTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa             >  2:246907/1‑141 (MQ=255)

                                                                                                                            ttA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTAAAGAAAATTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa             <  1:176941/141‑1 (MQ=255)

                                                                                                                            ttA‑CTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAACGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCGAACTATTAATGCaa             <  1:474716/141‑1 (MQ=255)

                                                                                                                            ttA‑CTAAACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa             <  2:347367/141‑1 (MQ=255)

                                                                                                                                   tactacAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAGATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaaa       <  2:776825/141‑1 (MQ=255)

                                                                                                                                        cAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAACCaat  <  2:244246/141‑1 (MQ=255)

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTA‑CTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAAT  >  CP000731/25523‑25799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`