Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,479 T→C 10.0% D1014D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4790TC10.0% 261.0 / 2.3 118D1014D (GAT→GACsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (57/42);  new base C (4/10);  total (63/55)
Fisher's exact test for biased strand distribution p-value = 4.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAT  >  USA300TCH1516_ALE/622339‑622611
                                                                                                                                             |                                                                                                                                    
gattccgaTAGCCTCTCAGATTCCCATAGAGAACCTGATTCGAACAGCGATTCAGACTCAGATAGCGACTCAGCTTTCGATAGCGGTTCTATATTCATCTGCGACAAAGTTTCAGATAGAGATTCGGACTCAGACAGCGAt                                                                                                                                      <  2:328026/141‑1 (MQ=9)
gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                                      <  1:462581/141‑1 (MQ=255)
  ttcagaCAGCGACTCAGCTTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCCGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                    <  2:535125/141‑1 (MQ=255)
  ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                    <  1:53323/141‑1 (MQ=255)
    cagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGGTTCGGACTCAGACAGCGATTCAg                                                                                                                                  >  1:333215/1‑141 (MQ=255)
      gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                                >  2:909777/1‑141 (MQ=255)
       aCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAGTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGAATCAGACTCAGACAGCGATTCCGAca                                                                                                                               >  2:468152/1‑140 (MQ=14)
        cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTTAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc                                                                                                                              <  2:623553/141‑1 (MQ=255)
           cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                           <  1:256099/141‑1 (MQ=255)
             aCTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATa                                                                                                                         >  1:799309/1‑141 (MQ=255)
                cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGAGTCTGGAGCCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCCGATTCAGATAGCGATTCGGACTCAGAAAGAGAATCAGCCTCAGATAGCg                                                                                                                      >  1:176328/1‑141 (MQ=14)
                cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                      >  1:281808/1‑141 (MQ=255)
                  gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                    <  1:719764/141‑1 (MQ=255)
                  aattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                    <  1:719773/140‑1 (MQ=255)
                   attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                                                                                   <  1:570795/141‑1 (MQ=255)
                    ttcagatAGCGACTCATATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                  >  1:504819/1‑141 (MQ=255)
                     tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactca                                                                                                                 >  1:563168/1‑141 (MQ=255)
                        gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCgacccagac                                                                                                              >  2:111684/1‑141 (MQ=255)
                        gatAGCGACTCAGAATCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                              >  1:501681/1‑141 (MQ=255)
                             cGACTCAGATGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                                                         <  1:824470/141‑1 (MQ=255)
                              gACTCAGATCCGGACAGCGATTCAGACTCAGATAGCGACTCTGATGCTGACACCGACACCGACACAGACACCGCCTCAGATTCACATACCGATCCCGACGCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                        <  2:756874/141‑1 (MQ=17)
                                   aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                                   <  1:485092/141‑1 (MQ=255)
                                    gatttcgACTGCGACTCAGACTCCGCAATGCACTCACATGCAGATAGCGATTCAGACTCAGCCAGCGTCTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  <  2:709939/135‑1 (MQ=11)
                                    gagtCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  <  1:772688/138‑1 (MQ=255)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  >  1:865456/1‑141 (MQ=255)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  >  1:18799/1‑141 (MQ=255)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTACGCCAGCGAc                                                                                                  >  1:261194/1‑141 (MQ=255)
                                    cactCGAACAGCGATTCAGACTCAGACACCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  <  2:839805/138‑1 (MQ=14)
                                     aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                                 <  1:698991/141‑1 (MQ=255)
                                     aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATGCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                                 <  1:894718/141‑1 (MQ=255)
                                      ttCGGACAGCGATTCACACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGAATCGGACTCAAACAACGAcca                                                                                                >  1:540755/1‑139 (MQ=14)
                                        cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAGAGCGACTCGGATTGAGAGAGCGACTGGGACTCAGAAGGCGATTGAGACCGAGATAGCGACTCAGACTGAGACGGCGCCgcag                                                                                              >  2:80551/1‑141 (MQ=14)
                                        cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAAAGACTCAGAATCCGATAGCGACACAGACCCAGACAGCAACtcag                                                                                              >  2:398808/1‑141 (MQ=14)
                                             aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattca                                                                                         <  1:789420/141‑1 (MQ=255)
                                             aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGCGACtcagacgaa                                                                                         >  2:540435/1‑137 (MQ=255)
                                                gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                                                                                      >  1:563605/1‑141 (MQ=255)
                                                 aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAAtcggattcaggaa                                                                                     >  2:14914/1‑138 (MQ=255)
                                                 aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACtcagattcagaaa                                                                                     >  2:724375/1‑139 (MQ=255)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTTAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                   <  2:499160/141‑1 (MQ=255)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCACACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                   <  2:584345/141‑1 (MQ=255)
                                                          cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                            <  2:737310/141‑1 (MQ=255)
                                                          cAGATAGCGACTCAGATTCAGATAGCGATTAAGATTCATACAGCGAATCCGACTCAGGAATCAAATCGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCGGACAGCGACTCAGATTCAGAAAGCGGTTAgg                                                                            >  2:414440/1‑141 (MQ=11)
                                                          aagagagCGATTCAGATTCAGATAGCGCTTCAGACTCACACTGCGACTCAGATTCAGAAAGCGATTCAGACTCAGACAGCGAGTCAGAATCAGATATCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                            <  2:426589/140‑1 (MQ=11)
                                                            gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAACGACTCAGATTCAGATAGCCACTCGGag                                                                          >  2:768522/1‑140 (MQ=255)
                                                             aTAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCCGACTCAGACAGCGACCCAGCCTCAGATAGCGACTCgggac                                                                         >  2:5196/1‑138 (MQ=14)
                                                             aTAGCGACTCAGATTCAGATAGCGAGTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGCTCGCGATTCGGCCt                                                                         >  1:219242/1‑141 (MQ=255)
                                                               aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACCAACCCAGATTCAGATAGCGATTCAGACTCa                                                                       >  2:38923/1‑141 (MQ=14)
                                                               aGCCACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTAAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                       <  1:804167/141‑1 (MQ=255)
                                                                gCGACTCAGATTCAGATAGTAATTCAGACTCAGACAGCGACTCAGATTCAGATAACGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCGAGACAGCGACTCAGATTCAGATATCGATTCGGACTCAg                                                                      >  1:590858/1‑141 (MQ=14)
                                                                gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                      >  1:767534/1‑141 (MQ=255)
                                                                gCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                      <  1:150021/141‑1 (MQ=255)
                                                                  gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                    <  1:468152/141‑1 (MQ=255)
                                                                      cagattcagatAGCGACTCAGACTCAGACATCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                <  2:579669/141‑1 (MQ=255)
                                                                      cacattccgatAGCGATTCAGCCCCAGACAGCGACTCAGATCCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                <  2:221467/138‑1 (MQ=17)
                                                                       aggttcggacAGCGAGTCAGATTCAGACAGCGACACAGATTCCGAGAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGCCTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                               <  2:789215/138‑1 (MQ=14)
                                                                       agatttagagAGCGATTCAGATTCAGACAGCGACTCAGATTGAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                               <  2:199143/141‑1 (MQ=14)
                                                                        gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                              >  1:15608/1‑141 (MQ=255)
                                                                         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGAAGTCATATAGCGAcc                                                             >  1:189007/1‑140 (MQ=255)
                                                                         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACACCGAcc                                                             >  2:310967/1‑140 (MQ=11)
                                                                              gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAt                                                        >  1:133794/1‑141 (MQ=255)
                                                                                tacccaTTCAGACTCAGACACCGACTCAGATTCTGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                      <  2:887646/136‑1 (MQ=11)
                                                                                   cGATTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                   <  1:208850/141‑1 (MQ=255)
                                                                                      ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                <  1:528437/141‑1 (MQ=255)
                                                                                        tgtggcgcagccagcGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGc                                               <  2:134330/134‑1 (MQ=14)
                                                                                           gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAACGAt                                            >  1:368336/1‑141 (MQ=255)
                                                                                           gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGACAGCggt                                            >  1:319346/1‑139 (MQ=255)
                                                                                           gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGac                                            >  2:904992/1‑140 (MQ=255)
                                                                                            caccagacAGCGACTCAGATTCAGCTAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGCAAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAACGACTCAGCAGCAGACAgagact                                           >  2:838552/4‑139 (MQ=14)
                                                                                            actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCAAtt                                           >  2:635133/1‑141 (MQ=255)
                                                                                              ccatacAGCGACTCAGATTCAGACAGCGATTCGCACTCAGACAGCGACTCAGACTCAGATAGCGACTCCGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCATATAGCGACGCAGAGTCAGACAGCGATTCa                                         <  2:180124/137‑1 (MQ=14)
                                                                                              ccagacAGCGACTCAGACTCAGACAGCGACTCGGATTCTGCGAGCGAGTCATACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCCGACAGCGATTCa                                         <  2:189281/140‑1 (MQ=14)
                                                                                                        acgccgattccggtAGGGATTCGGAATCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                               <  2:16637/136‑1 (MQ=17)
                                                                                                          tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCTGATAGCGACTCAGAGTCCGACAGCGAGTGAGACTcgggtggg                             >  2:48333/1‑134 (MQ=17)
                                                                                                          tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAAAGCGAATAAGACTCACATAGc                             >  2:285240/1‑141 (MQ=255)
                                                                                                           cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                            >  1:326262/1‑141 (MQ=255)
                                                                                                                 cagatAGCGATTCGGACTCAGGCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                      >  1:473029/1‑141 (MQ=255)
                                                                                                                 cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                      >  1:473084/1‑141 (MQ=255)
                                                                                                                 cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                      <  1:493556/141‑1 (MQ=255)
                                                                                                                    atAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagacc                   >  1:849567/1‑140 (MQ=255)
                                                                                                                     aaGCGACTCCGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                  <  2:260458/140‑1 (MQ=255)
                                                                                                                      cccGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGGCTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                 <  2:162100/139‑1 (MQ=255)
                                                                                                                        cGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga               >  1:340841/1‑141 (MQ=255)
                                                                                                                         aaagcagACTCAGACAGCGACTCAGACGCAGAAAGCGAATCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac              <  2:510109/135‑1 (MQ=14)
                                                                                                                           ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGATAGCGACTCAGATTCAGAAAGCGATACAGACTCAGATAGCGACTcagacacagacaa            >  2:659819/1‑140 (MQ=255)
                                                                                                                            tctgccgcAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCCCACAGCGATTCAGACTCAGATAGCCACTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt           <  2:902145/134‑1 (MQ=11)
                                                                                                                             cGGCCTCAGACAGCTACTCCGACTCAGACAGCGACTCCGACTCAGACAGCGACTCCGATTCAGGAAGCGATTCCGACCCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          <  2:431366/141‑1 (MQ=14)
                                                                                                                             cGGCCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          <  1:105024/141‑1 (MQ=255)
                                                                                                                             cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGCCAGTg          >  1:364966/1‑141 (MQ=255)
                                                                                                                             cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          >  1:278841/1‑141 (MQ=255)
                                                                                                                             cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          <  1:882917/141‑1 (MQ=255)
                                                                                                                               gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACGGCGACTCAGAGTGTGATAGCGATTCGGACTCAGATAGCGCATCAGATTCGGACAGCGATTCGGATTCTGGTAGCGGGTGAGACGTAGAGAGTggt        >  2:148582/1‑139 (MQ=14)
                                                                                                                               gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat        >  1:105261/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt       >  1:913874/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACACAGACAGTgatt       >  2:438689/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAAACCCCGACAGTgatc       >  2:161650/1‑140 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTAACACGGCGACTCAGACTAGGATAGCGGCTCAGACGCAGACAgagaat       >  2:544173/1‑139 (MQ=14)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCgatt       >  2:487290/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATACAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt       >  1:486959/1‑141 (MQ=255)
                                                                                                                                  tCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattca     >  2:764695/1‑141 (MQ=255)
                                                                                                                                     gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACTgattcagat  >  1:905033/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                    
GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAT  >  USA300TCH1516_ALE/622339‑622611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: