Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 628,618 T→A 6.9% S8T (TCG→ACG)  nagB_1 → Glucosamine‑6‑phosphate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE628,6180TA 6.9% 250.5 / 3.5 97S8T (TCG→ACG) nagB_1Glucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base T (52/37);  new base A (3/5);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCAAAAATTTGTTGGAATTTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGTTATTAAATAAAAAT  >  USA300TCH1516_ALE/628478‑628758
                                                                                                                                            |                                                                                                                                            
aaTCAAAAATTTGTTGGAATTTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGAt                                                                                                                                              >  2:456073/1‑141 (MQ=255)
  acAAAAATTTGTTGGAATGTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCg                                                                                                                                            <  1:579036/140‑1 (MQ=255)
   cAAAAATTTGTTGGAATTTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAAATAGGGATCGa                                                                                                                                           >  2:423688/1‑141 (MQ=255)
    aaaaaTTTGTTGGAATTTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGaa                                                                                                                                          <  1:716075/141‑1 (MQ=255)
        aTTTGTTGGAATTTGAAAAGTGTTAAACATGAAAAATGATGCTATATTAATGGTGTATGAATGAAATCATAAGATTTTAAAATGTAGTGAATTGGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGTGTCGaaaaaa                                                                                                                                      >  2:210252/1‑141 (MQ=255)
          ttgttgGAATTTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACa                                                                                                                                    >  2:842299/1‑141 (MQ=255)
                     tGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGACTTAATAAGTTTTTAACATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAATATTAAACTTAGGAACGAAATAACAAGCATCATTca                                                                                                                         >  2:626911/1‑140 (MQ=255)
                     tGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGAATCATTCt                                                                                                                         >  2:463809/1‑141 (MQ=255)
                        aaaGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTATACTTAGGATCGAAAAAACAAGCATCATCCTATg                                                                                                                      >  1:106750/1‑141 (MQ=255)
                            tgtTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGc                                                                                                                  >  2:92153/1‑141 (MQ=255)
                              ttAAACATTTAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCAt                                                                                                                <  2:875105/141‑1 (MQ=255)
                              atAAACATTAAAAATGAAGTGATATTAATGGTGTAAAAATGAATAAATAAGATGTTAAAATGTATTAAACTTGTGGAGGAATTGCAAGACTGAATGTATTAAAAAAAAAAAAAAAAAAACAAGCATCATTCTATGTTGCAt                                                                                                                <  2:229339/140‑1 (MQ=255)
                               tAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCAtg                                                                                                               <  1:804833/141‑1 (MQ=255)
                                aaaCATTAAAAATGATGTTATATTAATGGTGTATGAATGAATTAATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACGATGTATGTATTAAAAAAAAAAAAAAAAAAACAAGCATCATTCTATGTTGCAtgt                                                                                                              <  2:118767/141‑1 (MQ=255)
                                aaaCATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAATTTTTTAAGATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCAtgt                                                                                                              <  1:671853/141‑1 (MQ=255)
                                aaaCATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCAtgt                                                                                                              <  1:820209/141‑1 (MQ=255)
                                     ttAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGtt                                                                                                         <  1:98090/141‑1 (MQ=255)
                                       aaaaaTGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTTTTTTTTTTTAAATTTGTGGAGGCATGTAAACAATGAAAATATTAAACTTAGGTTAGAAAAAACATGCATCATTCTATTTTGCATGTAAGTtat                                                                                                       >  2:600209/1‑141 (MQ=255)
                                         aaaTGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGGGAGTtatat                                                                                                     >  2:574109/1‑141 (MQ=255)
                                          aaggatgGTAAATAAAAGGTGTAGGAATGAATACAAAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACGATGAAAGTATTAAAAAAAAAAAAAAAAAAACAAGCATCATTCTATGTTGCATGTGAGTtatata                                                                                                    <  2:66102/138‑1 (MQ=255)
                                          aaTGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTtatata                                                                                                    >  1:222633/1‑141 (MQ=255)
                                             gatgCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAag                                                                                                 >  2:560186/1‑141 (MQ=255)
                                              atgCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAagg                                                                                                >  2:738568/1‑140 (MQ=255)
                                                  tataTTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATg                                                                                            >  1:128574/1‑141 (MQ=255)
                                                  tataTTAATGGTGTATGAATGAATTCATAAGTATTTAAAATGTATTAAACTTGTGTAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATg                                                                                            >  1:128492/1‑141 (MQ=255)
                                                   atatTAATTGTGTATGAATGAATTTATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATgg                                                                                           <  2:393445/141‑1 (MQ=255)
                                                      ttAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCAt                                                                                        >  1:20763/1‑141 (MQ=255)
                                                        aaTGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCAttt                                                                                      <  1:319958/141‑1 (MQ=255)
                                                         aTGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTa                                                                                     <  2:118177/141‑1 (MQ=255)
                                                         aTGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTa                                                                                     <  1:537450/141‑1 (MQ=255)
                                                          tGGTTTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGTGTTATATAAAGAGATGGCATTTaa                                                                                    <  1:572436/141‑1 (MQ=255)
                                                           ggTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAGCAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAAt                                                                                   >  1:879700/1‑141 (MQ=255)
                                                            gtgtATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATc                                                                                  <  2:277462/141‑1 (MQ=255)
                                                            gtgtATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAAATTAAGAAAGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATc                                                                                  <  2:277489/141‑1 (MQ=255)
                                                                atgaatgaATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCa                                                                              >  2:364196/1‑141 (MQ=255)
                                                                    atgaatTCATAAGTTTTTACAATGTATTAAATTTGTGGAGGCATGTCAACAATGAAAGTATTAAACTTAGGATCGCAAAAACCAGCATCATTCTATCTTGCTTGTGAGTTATATAAAGAGATGGCATTTAATCATCACTGt                                                                          >  2:366603/1‑141 (MQ=255)
                                                                    atgaatTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTAATAAACTTAGGAACGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGt                                                                          >  2:863284/1‑141 (MQ=255)
                                                                      taatTCATAAGTTTTTAAAATGTATTAAAATTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCAGTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTaa                                                                        <  2:546407/140‑1 (MQ=255)
                                                                      gaatTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATCAAGAGATGGCATTTAATCAGCACTGTaa                                                                        >  1:634197/1‑141 (MQ=255)
                                                                      gaatTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTaa                                                                        <  2:353856/141‑1 (MQ=255)
                                                                      gaatTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATAAACACTGTaa                                                                        >  2:122826/1‑141 (MQ=255)
                                                                            aTAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAgg                                                                  >  2:733907/1‑141 (MQ=255)
                                                                            aTAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAgg                                                                  >  1:320014/1‑141 (MQ=255)
                                                                            aTAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAgg                                                                  >  1:251344/1‑141 (MQ=255)
                                                                            aTAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAgg                                                                  >  1:251608/1‑141 (MQ=255)
                                                                            aTAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCAAAGTAAACTAgg                                                                  >  1:698223/1‑141 (MQ=255)
                                                                            aTAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCAAGTGAGTTATATAAAGAGATGGCAATTAATCAGCACTGTAAACTAgg                                                                  >  2:399659/1‑141 (MQ=255)
                                                                              aaGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGtt                                                                <  1:419377/141‑1 (MQ=255)
                                                                              aaGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGATATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGtt                                                                >  2:726124/1‑141 (MQ=255)
                                                                                  ttttAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGc                                                            <  2:803540/141‑1 (MQ=255)
                                                                                  ttttAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGc                                                            >  1:795543/1‑141 (MQ=255)
                                                                                  ttttAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGc                                                            <  1:500202/141‑1 (MQ=255)
                                                                                  atgtAAAATGTATTAAATTTGTGGAAGCAAGTAAACAATGAAAATATTAAACATAGTATCGAAAAAACAAGCATCAATCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGc                                                            <  2:449707/138‑1 (MQ=255)
                                                                                   tttAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCa                                                           >  2:341286/1‑141 (MQ=255)
                                                                                     tAAAATTTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGTTCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAAc                                                         <  1:510276/141‑1 (MQ=255)
                                                                                     tAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAAc                                                         >  2:793065/1‑141 (MQ=255)
                                                                                     tAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAAc                                                         <  1:33141/141‑1 (MQ=255)
                                                                                      aaaaTGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACt                                                        >  2:768971/1‑141 (MQ=255)
                                                                                      aaaaTGTATTAAATTTGTGGAAGCATGTAAACAATGAAAGTATTAAAAAAAAAAAAAAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACt                                                        <  2:640748/141‑1 (MQ=255)
                                                                                        aaTGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCGGCCCTGTAAACTAGGTTTAGCAACtgg                                                      >  1:562290/1‑141 (MQ=255)
                                                                                        aaTGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACtgg                                                      <  1:274710/141‑1 (MQ=255)
                                                                                          tGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACtggtg                                                    >  2:692131/1‑141 (MQ=255)
                                                                                            tATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAAATAGGTTTAGAACAtggtgtg                                                  >  1:97334/1‑139 (MQ=255)
                                                                                            tATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGCAAACTAGGTTTAGCAACTggttgt                                                  <  2:181458/141‑1 (MQ=255)
                                                                                              ttAAATTTGTGGAGGCATGTAAACAATGTAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTAc                                                <  2:144582/141‑1 (MQ=255)
                                                                                              ctcaacttGTGGAGGCATTTAAACAATGAAAGTATCAAACTTAGGATCGACAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGACGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTAc                                                <  1:66108/135‑1 (MQ=255)
                                                                                                aaaTTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACaa                                              >  2:773667/1‑141 (MQ=255)
                                                                                                aaaTTAGTGAAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACaa                                              >  2:556681/1‑141 (MQ=255)
                                                                                                 aaTTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAAt                                             <  1:384949/141‑1 (MQ=255)
                                                                                                  aTTTGTGGAGGCATGTAAAAAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATg                                            >  1:497727/1‑141 (MQ=255)
                                                                                                    ttGTGTAGGCGTGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGAc                                          <  2:677989/141‑1 (MQ=255)
                                                                                                     tgtgGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACa                                         >  1:657031/1‑141 (MQ=255)
                                                                                                         gAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGAtt                                     >  2:614804/1‑141 (MQ=255)
                                                                                                          aGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGAttt                                    >  1:383969/1‑141 (MQ=255)
                                                                                                            gCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGt                                  >  1:565388/1‑141 (MQ=255)
                                                                                                              aTGAAAACAATTAAAGTAATAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTAt                                <  2:659017/141‑1 (MQ=255)
                                                                                                                gTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGa                              >  1:36741/1‑141 (MQ=255)
                                                                                                                gTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCAGTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGa                              >  1:572416/1‑141 (MQ=255)
                                                                                                                 taaatcaTGAACGTATTCAAAATAGGATCGACAACCCAAGCATACTTCTATGATGCAAGTGATGTGTAAAACGAGATGGCAATTAATAAGCACTGTAAACAGGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGaa                             >  2:264859/7‑141 (MQ=255)
                                                                                                                 aaaaTAATGAAAGGATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGaa                             <  1:767283/140‑1 (MQ=255)
                                                                                                                     cAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGaacaac                         >  1:427161/1‑141 (MQ=255)
                                                                                                                      aaTGAAAGTATTAAACCTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACt                        <  1:296658/141‑1 (MQ=255)
                                                                                                                       aggAAAGTAATAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACtt                       <  2:321518/139‑1 (MQ=255)
                                                                                                                       aTGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAACCTCGGTTTAGAAACTGGTGGCACACTGACCGACTTACATGTCCAACtt                       <  2:906081/141‑1 (MQ=255)
                                                                                                                       aTGAAAGAATTAAAGTCAGGATCGAAAAAACAAGCATCATTCTAGGTTGCATGTGAGTTATATAAAGGGATGGCATTTAAGCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACtt                       <  2:428195/141‑1 (MQ=255)
                                                                                                                        tGAAAGTATTAAACTTAGGATCGAAAAAATAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTg                      <  2:189818/141‑1 (MQ=255)
                                                                                                                        tGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTg                      >  2:360909/1‑141 (MQ=255)
                                                                                                                            aGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTTGCGGTACAATGACAGATTTGTATGAACAACTTGTTaa                  >  2:866996/1‑141 (MQ=255)
                                                                                                                            aGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTaa                  <  1:593744/141‑1 (MQ=255)
                                                                                                                            aGTATTAAACTTAGGAACGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAAGTAGGTTTAGCAACTGGTGGTACAATGAGAGATTTGTATGAACAACGTGTTaa                  >  1:450686/1‑141 (MQ=255)
                                                                                                                               aTTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGtt               >  1:854147/1‑141 (MQ=255)
                                                                                                                                  aaaCTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGCCAGATTTGTATGAACAACTTGTTAAGttatt            >  2:758161/1‑141 (MQ=255)
                                                                                                                                   aaCTTAGGATCTAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGttatta           <  1:477309/141‑1 (MQ=255)
                                                                                                                                   aaCTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGttatta           >  2:617966/1‑141 (MQ=255)
                                                                                                                                   aaCTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGttatta           >  1:741529/1‑141 (MQ=255)
                                                                                                                                     cTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGTTATtaaa         <  1:768971/141‑1 (MQ=255)
                                                                                                                                         ggATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGTTATtaaataaa     >  1:716309/1‑141 (MQ=255)
                                                                                                                                           aTCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGGGGTACAATGACAGATTTGTATGAACAAATTGTTAATTTATTCAATaaaaa   >  1:328941/1‑141 (MQ=255)
                                                                                                                                            tCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGTTATTAAATAAAAAt  >  2:707410/1‑141 (MQ=255)
                                                                                                                                            tCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGTTATTAAATAAAAAt  >  2:76723/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AATCAAAAATTTGTTGGAATTTGAAAAGTGTTAAACATTAAAAATGATGCTATATTAATGGTGTATGAATGAATTCATAAGTTTTTAAAATGTATTAAATTTGTGGAGGCATGTAAACAATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTATATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATGACAGATTTGTATGAACAACTTGTTAAGTTATTAAATAAAAAT  >  USA300TCH1516_ALE/628478‑628758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: