Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,002,200	T→G	100%	M173R (ATG→AGG)	mecA →	Adapter protein MecA 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,002,200	0	T	G	100.0%	225.1 / NA	64	M173R (ATG→AGG)	mecA	Adapter protein MecA 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/27);  total (37/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTA  >  USA300TCH1516_ALE/1002063‑1002337                                                                                                                                          |                                                                                                                                           aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                         <  2:542957/141‑1 (MQ=255)  ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                        <  2:424877/141‑1 (MQ=255)  ggTTCTAAACGTCAAAAGACTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                        <  1:883723/141‑1 (MQ=255)   gTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATCGCAACCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTg                                                                                                                                       <  1:480541/141‑1 (MQ=255)   gTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTg                                                                                                                                       <  2:164194/141‑1 (MQ=255)    ttCGAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGa                                                                                                                                      <  2:914495/141‑1 (MQ=255)        aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                                  >  2:119416/1‑141 (MQ=255)        aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                                  >  2:666690/1‑141 (MQ=255)           cGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACt                                                                                                                               >  2:139436/1‑141 (MQ=255)           cGTCAAAAGTCTTCAGAACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACt                                                                                                                               >  1:465786/1‑141 (MQ=255)               aaaaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGTTACttatt                                                                                                                           >  1:415353/1‑141 (MQ=255)               aaaaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                                                                           <  2:148396/141‑1 (MQ=255)               aaaaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                                                                           >  1:820215/1‑141 (MQ=255)               aaaaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                                                                           <  2:266084/141‑1 (MQ=255)                aaaGTCTTCAGCACGTAAAAATCCAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatta                                                                                                                          <  1:549034/141‑1 (MQ=255)                 aaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTGCttattat                                                                                                                         >  2:231361/1‑141 (MQ=255)                        cAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGCTATATAGGGTTGATGGTAGTTATTATTATGCTg                                                                                                                  >  2:712064/1‑141 (MQ=255)                                   aaTACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGAt                                                                                                       <  1:565874/141‑1 (MQ=255)                                          gAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATg                                                                                                >  2:70185/1‑141 (MQ=255)                                           aaCATTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCAGAGCAATCCAATAACTTCAGCGTTTCAAGATTTGTTATATTGGGATGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGt                                                                                               >  2:885202/1‑141 (MQ=255)                                           aaCAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTAATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGt                                                                                               >  1:563225/1‑141 (MQ=255)                                           aaCAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAAATTTGTTATATAGGGTTGATGGTACTTATTATTATACTTTATATTTTGATAGTCAttt                                                                                               >  2:300562/1‑139 (MQ=255)                                              aataatTGTGAAATGTAACGCTTTAGAAGATGTTATTAATTATGCATATAATAGCAATCCCATAACTACAGAGTTTGAAGATTTGTTATATAGCAGTGCTGGTACAGATTATTATGCTGTATATTTTTATAGTCGTGTTGa                                                                                            >  2:412896/1‑141 (MQ=255)                                               aTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                           >  2:75230/1‑141 (MQ=255)                                                tCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATc                                                                                          <  2:455926/141‑1 (MQ=255)                                                 cATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                         >  1:729516/1‑141 (MQ=255)                                                 cATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                         >  1:464292/1‑141 (MQ=255)                                                 cATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                         >  2:743369/1‑141 (MQ=255)                                                   ttGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaag                                                                                       <  2:885154/141‑1 (MQ=255)                                                     gtgAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                                                                     <  1:481631/141‑1 (MQ=255)                                                       taaaTTTAATGATTTTGAAGAGGTGATTAATTATGCATATGCTATGAGTCAGATAACTCCATAGTTTGTAGATTTTTTATATAGGGTTGAGGGTACTTATTATTATGCTGTATATTTCGTTAGTCATGTTGATCAAGAGGt                                                                                   <  2:249221/140‑1 (MQ=255)                                                       gAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGt                                                                                   <  2:627125/141‑1 (MQ=255)                                                         aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                                 >  2:277027/1‑141 (MQ=255)                                                         aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                                 >  2:703654/1‑141 (MQ=255)                                                          aTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGGTAGTCATGTTGTTCAAGAAGTCAt                                                                                >  1:528017/1‑141 (MQ=255)                                                            ttAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTa                                                                              <  1:139436/141‑1 (MQ=255)                                                             tAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTaa                                                                             <  1:723390/141‑1 (MQ=255)                                                               aCGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTTATAGTCATGTTGATCAAGAAATCAtgcatg                                                                           >  1:704408/1‑136 (MQ=255)                                                                       gaagaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTAc                                                                  >  2:914069/1‑141 (MQ=255)                                                                              ttattaATTATGCATATCATAGCAGTCCAACAACTACCGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGGATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAAt                                                           <  1:148052/141‑1 (MQ=255)                                                                                     ttaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTAAAGTCAATTGCTTGa                                                    >  2:181176/1‑141 (MQ=255)                                                                                          cATATCATAGCAATCCAATAACTACAGATTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTg                                               <  2:624519/141‑1 (MQ=255)                                                                                          cATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTCTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTg                                               <  2:462251/141‑1 (MQ=255)                                                                                              tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCttt                                           >  1:577206/1‑140 (MQ=255)                                                                                              tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTa                                           >  1:473339/1‑141 (MQ=255)                                                                                                aTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATc                                         >  2:264670/1‑141 (MQ=255)                                                                                                aTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATc                                         >  2:456077/1‑141 (MQ=255)                                                                                                   gCAACCCCATTACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATATTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCaa                                      <  1:195189/141‑1 (MQ=255)                                                                                                    cAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAac                                     <  1:70185/141‑1 (MQ=255)                                                                                                       tCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCACGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAacaga                                  >  2:498112/1‑141 (MQ=255)                                                                                                          aaTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATAAT‑AGTTACAGTCAATTGCTTGAATTTGCTTAGCAAacacacag                               >  2:23282/1‑141 (MQ=255)                                                                                                           aTAACTACAGAGTTTGAAGATTTGTAATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                              >  1:455976/1‑141 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             >  2:83696/1‑141 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             >  1:363952/1‑141 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             <  1:75230/141‑1 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             >  1:622915/1‑141 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             >  1:634929/1‑141 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             >  1:648560/1‑141 (MQ=255)                                                                                                            tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaa                             >  1:84858/1‑141 (MQ=255)                                                                                                                   agagTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTAGGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGt                      <  1:168317/141‑1 (MQ=255)                                                                                                                         tGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGtttattt                <  1:743369/141‑1 (MQ=255)                                                                                                                                 tGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACt        <  1:277027/141‑1 (MQ=255)                                                                                                                                 tGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACt        <  1:703654/141‑1 (MQ=255)                                                                                                                                    tatataGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATg     >  1:52137/1‑141 (MQ=255)                                                                                                                                     atatagGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCGATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGc    <  1:119416/141‑1 (MQ=255)                                                                                                                                     atatagGGTTGATGGTACTTATTATTAAGCTGTATACATAGAACCACATGTTGAGCAAGAAGTC‑TTTAGGATGTGAGACAGTCCCTTATTTAATTGTTCTTTTCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGc    <  1:666690/141‑1 (MQ=255)                                                                                                                                       atagGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTa  <  1:555956/141‑1 (MQ=255)                                                                                                                                       atagGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTa  >  1:223875/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                           AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAT‑AGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTA  >  USA300TCH1516_ALE/1002063‑1002337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑