BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,771,347	C→A	5.9%	P49Q (CCA→CAA)	phoB →	Alkaline phosphatase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,771,347	0	C	A	5.9%	359.1 / 2.9	112	P49Q (CCA→CAA)	phoB	Alkaline phosphatase 3
Reads supporting (aligned to +/- strand): ref base C (55/48); new base A (2/6); total (57/55)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTAC  >  USA300TCH1516_ALE/2771208‑2771485

tCAGTTAAAAAAGCCACATAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGTTAATACTAAAAATCCa                                                                                                                                           <  1:449987/141‑1 (MQ=255)

 cAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTCAAAATCCaa                                                                                                                                          >  1:582078/1‑141 (MQ=255)

 cAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGGTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCaa                                                                                                                                          >  2:668838/1‑141 (MQ=255)

   gTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCaaaa                                                                                                                                        <  1:351174/141‑1 (MQ=255)

    ttAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCaaaaa                                                                                                                                       >  1:154812/1‑141 (MQ=255)

      aaacaaGCTCCAAAATTTGCAGTTGTTCACCTTCTTATTGTGCCAACGGTTTTTACGTCTGTCCCCCCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAAt                                                                                                                                     <  1:803602/141‑1 (MQ=255)

      aaaaaaGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTCCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAAt                                                                                                                                     <  1:896470/141‑1 (MQ=255)

        aaaaGCCACAACATTTGCAGTTGCTAACCTTATTATCGTGTCACCGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGTTAGAAGTTCTAAAGATGAACGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGt                                                                                                                                   <  2:594064/141‑1 (MQ=255)

         aaaGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGtt                                                                                                                                  >  1:280821/1‑141 (MQ=255)

               acaAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCGAAAAATGTTATCttt                                                                                                                            <  1:916659/141‑1 (MQ=255)

               acaAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCttt                                                                                                                            <  2:415922/141‑1 (MQ=255)

                caAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCtttt                                                                                                                           <  2:706370/141‑1 (MQ=255)

                aaaaaTTTGCAGTTGCTAACCTTAATATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATAAAAAAAAAAAAAAAAATGTTATCtttt                                                                                                                           <  2:706340/140‑1 (MQ=255)

                   aaTTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAg                                                                                                                        <  1:320011/141‑1 (MQ=255)

                   aaTTTGCAGTAGCTAACATTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCGGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATAAAAAAAAAAAAAAAAATGTTATCTTTTTAg                                                                                                                        <  2:735740/141‑1 (MQ=255)

                    aTTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGACGAAACCTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGt                                                                                                                       <  1:479537/141‑1 (MQ=255)

                     tttGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGtt                                                                                                                      >  1:597544/1‑141 (MQ=255)

                         cAGTTGCTAACCTTATTCTCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCg                                                                                                                  <  1:815806/141‑1 (MQ=255)

                         cAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAGGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCg                                                                                                                  <  1:590153/141‑1 (MQ=255)

                         cAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCCTTGAATGTTATCTTTTTAGTTGGCg                                                                                                                  <  1:219016/141‑1 (MQ=255)

                           gTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGAt                                                                                                                >  1:513482/1‑141 (MQ=255)

                           gTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATATTTTTATTTGGCgtt                                                                                                                >  1:8293/1‑139 (MQ=255)

                             tGCTACCCTTATTATCGTGTCAACGGTTTTTAGTCCTGTCACACCTGCAATTGCGCGATCCGATAAAAGTTCTAAAGATGAAAGTTTCGTGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATgg                                                                                                              <  2:443015/141‑1 (MQ=255)

                               cTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCa                                                                                                            <  2:56715/141‑1 (MQ=255)

                               cTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCa                                                                                                            <  2:271819/141‑1 (MQ=255)

                                caaCCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCAt                                                                                                           <  1:152206/140‑1 (MQ=255)

                                  aCCTTATTATCGTGTCAAGGGTTTTTGGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTGGTTGGCGATGGCATgg                                                                                                         >  2:235911/1‑141 (MQ=255)

                                  aCCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCCTgg                                                                                                         >  2:53652/1‑141 (MQ=255)

                                    cTTGTTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTGCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGt                                                                                                       <  2:49225/141‑1 (MQ=255)

                                    cTTATTTTCGTGGCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGt                                                                                                       <  1:223603/141‑1 (MQ=255)

                                     ttattaTCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAGAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTc                                                                                                      >  1:303810/1‑141 (MQ=255)

                                      tattctCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTcc                                                                                                     <  1:342362/141‑1 (MQ=255)

                                      tattatCTTTTCAACGGTTTTTACTTCTGTCACACCTGCAATTGCGCAATCCGATAAACGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTTGTTGGCGATGGCTTGGGTcc                                                                                                     <  1:573450/141‑1 (MQ=255)

                                      tattatCGTGTGAACGGTTTTTAGTTCTGTCACACCAGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACAAAAAAAAAAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTcc                                                                                                     <  2:537881/141‑1 (MQ=255)

                                       attatCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTccc                                                                                                    >  2:715043/1‑140 (MQ=255)

                                            cGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCAtt                                                                                               >  1:735792/1‑141 (MQ=255)

                                            cGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCAtt                                                                                               <  1:885419/141‑1 (MQ=255)

                                            cGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCAtt                                                                                               >  2:561059/1‑141 (MQ=255)

                                             gtgtCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCAttt                                                                                              <  2:174578/141‑1 (MQ=255)

                                              tgtCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCAATAATTTa                                                                                             >  2:382049/1‑141 (MQ=255)

                                              tgccAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTa                                                                                             <  1:629697/138‑1 (MQ=255)

                                                 cAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAaca                                                                                          >  1:751895/1‑141 (MQ=255)

                                                  aaCGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGAGGGCATGGGTCCCTCATTTAacca                                                                                         >  2:559337/1‑139 (MQ=255)

                                                    cGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAg                                                                                       <  2:104835/141‑1 (MQ=255)

                                                    cGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAg                                                                                       <  1:139725/141‑1 (MQ=255)

                                                         tttAGTTCTGTCACACCTGCAATTGCGCAATCCGGTAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTATTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTAt                                                                                  >  2:638380/1‑141 (MQ=255)

                                                           tAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATAAAAAAAAAAAAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACAAAGCATATct                                                                                <  2:790299/141‑2 (MQ=255)

                                                           tAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGCTGGTAATACTAAAAATCCAAAAACTGTTATCTTTTTAGTTGGCGATGGCATGGGACCCTCCTTTAACACAGCTTATCg                                                                                >  2:297794/1‑141 (MQ=255)

                                                                cTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTATCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTCTCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGttatt                                                                           >  2:672513/1‑141 (MQ=255)

                                                                  gTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTtattat                                                                         >  2:10216/1‑141 (MQ=255)

                                                                      cacCTGCAATTGCGCAATCCGATAAAAGTTCGAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATaaaa                                                                     <  2:550518/141‑1 (MQ=255)

                                                                       acCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATaaaaa                                                                    <  2:29710/141‑1 (MQ=255)

                                                                       acCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATaaaaa                                                                    >  2:204707/1‑141 (MQ=255)

                                                                       aacTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATaaaaa                                                                    <  2:549609/139‑1 (MQ=255)

                                                                          tGCAATTGCGCAAACCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGa                                                                 <  1:324434/141‑1 (MQ=255)

                                                                           gCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTACCACAGCTTATCGTTATTATAAAAATGac                                                                >  2:314816/1‑140 (MQ=255)

                                                                           gCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGAt                                                                >  2:495820/1‑141 (MQ=255)

                                                                           gCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGAt                                                                >  2:442655/1‑141 (MQ=255)

                                                                              aTTGCGCAATCCGATAAAAGTTTTTTGAATTAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCg                                                             >  1:906941/1‑141 (MQ=255)

                                                                              aTTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCg                                                             <  2:36147/141‑1 (MQ=255)

                                                                              aTTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCg                                                             <  2:784312/141‑1 (MQ=255)

                                                                              aTTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCg                                                             <  2:513482/141‑1 (MQ=255)

                                                                               ttGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAACTCAAAAAAATGCTATCTTTTTAGTCGGCGAGAGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGc                                                            >  2:667036/1‑141 (MQ=255)

                                                                                tGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACCAAAACTCCAAAAAATGTTATCTTTTTAGTTGGCGATGGGATGGGTCCATCATTTAACCCACCTTCACCTTATTATGAACATGACCCGcc                                                           >  2:711718/1‑140 (MQ=255)

                                                                                     aaTCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATAATCCGCACGCTa                                                      >  1:370405/1‑141 (MQ=255)

                                                                                         cGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGa                                                  <  1:10216/141‑1 (MQ=255)

                                                                                           aTAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGaaa                                                >  1:594731/1‑141 (MQ=255)

                                                                                               aaGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCAAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGAc                                            <  1:296531/141‑1 (MQ=255)

                                                                                                 gTTCTAAAGATGAAAGTTTCGCGGTTGGTCATAGTAAAAATCCACAAAATGTGAGCTTTTGAGTTTGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGAccc                                          >  2:643024/1‑141 (MQ=255)

                                                                                                 gTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTCAAGAAATGAccc                                          >  1:528219/1‑141 (MQ=255)

                                                                                                  ttCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCACAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGCACCGCATGCTAAAGAAACGAccac                                         >  2:383469/1‑139 (MQ=255)

                                                                                                  ttCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGAcccc                                         >  2:461714/1‑141 (MQ=255)

                                                                                                   tCTAAAGATGAAAGTTGCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCt                                        <  2:61734/141‑1 (MQ=255)

                                                                                                    tgaaaGATGAAAGTTTCACGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTGGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTGTAAAAATGATCCGCATGCTAAAGAAATGACCCCTa                                       <  2:278462/139‑1 (MQ=255)

                                                                                                    ccaaaGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGCTGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTa                                       <  2:678714/139‑1 (MQ=255)

                                                                                                        aGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGATATCTGTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATAATCCGCATGCTAAAGAAAGGCCCCCTACTgg                                   >  1:414087/1‑140 (MQ=255)

                                                                                                         gATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCt                                  <  1:347339/141‑1 (MQ=255)

                                                                                                         gATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCt                                  <  2:280821/141‑1 (MQ=255)

                                                                                                             aaaGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTCAGCCAGCGTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTg                              >  1:662657/1‑141 (MQ=255)

                                                                                                              aaGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTCGTTGGCGATGGCATGGGTCCATCATTTAACACAGATAAATTTTAGTATAAAAATGATCCGCATGCTAAAGAAATGACCCCGACTGCTTTTGa                             >  2:641582/1‑141 (MQ=255)

                                                                                                              aaGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGCCCCCTACTGCTTTTGa                             >  2:266976/1‑141 (MQ=255)

                                                                                                              aaGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACAAAGATTTGCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGa                             >  1:431055/1‑141 (MQ=255)

                                                                                                                 tttCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTc                          >  1:459197/1‑141 (MQ=255)

                                                                                                                 tttCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTc                          >  1:179700/1‑141 (MQ=255)

                                                                                                                  ttCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCa                         >  2:792124/1‑141 (MQ=255)

                                                                                                                  ttCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCa                         >  2:695053/1‑141 (MQ=255)

                                                                                                                  ttCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCa                         >  1:237271/1‑141 (MQ=255)

                                                                                                                   tCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTATTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCat                        >  2:486363/1‑141 (MQ=255)

                                                                                                                   tCGCGGTTGGTAATACTAAAAATCAAAAAAATGTTATCTTTTTAGTTGGAGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCat                        >  1:665943/1‑141 (MQ=255)

                                                                                                                    cgcgGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTCAAGAAATCACCCCTACTGCTTTTGATTctat                       >  2:355506/1‑138 (MQ=255)

                                                                                                                           ggTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGCTGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATACTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTaaaa                >  2:6860/1‑141 (MQ=255)

                                                                                                                               aTACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGca            >  1:385179/1‑141 (MQ=255)

                                                                                                                               aTACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGca            >  1:357083/1‑141 (MQ=255)

                                                                                                                               aTACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGca            <  1:359690/141‑1 (MQ=255)

                                                                                                                                tACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGcac           <  2:834626/141‑1 (MQ=255)

                                                                                                                                caCTAAATATCGCAAAAATGTTATGTTTTTAGGTGGCGCTGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCAGCTTTAAAAGGcac           <  2:297000/140‑1 (MQ=255)

                                                                                                                                     aaaaTCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATc      <  1:16878/141‑1 (MQ=255)

                                                                                                                                         tCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTGATATTTAAAGGGCACAAATCGTAc  >  2:711996/1‑141 (MQ=255)

                                                                                                                                         tCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTAc  >  2:513497/1‑141 (MQ=255)

                                                                                                                                         tCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTAc  <  1:495820/141‑1 (MQ=255)

TCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACGGTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATGACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTAC  >  USA300TCH1516_ALE/2771208‑2771485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`