BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,969	A→G	6.8%	D739D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,969	0	A	G	6.8%	317.6 / 4.1	128	D739D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (59/56); new base G (3/9); total (63/65)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTG  >  USA300TCH1516_ALE/2774829‑2775105

cTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGaa                                                                                                                                           <  2:699834/141‑1 (MQ=255)

  gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                         <  1:374544/141‑1 (MQ=255)

  gTCCGAATCTTAATCACTGTCTGAATGCGAATCGCCATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                         <  1:847969/141‑1 (MQ=255)

      gaatctgaatcACTGTCTGGATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGCCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                                                                                                                     <  1:4945/141‑1 (MQ=255)

      gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCtt                                                                                                                                     >  1:721536/1‑140 (MQ=255)

         tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCCAATCGCGATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                                                                                                                                  >  2:411801/1‑141 (MQ=17)

            gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                               >  1:30930/1‑141 (MQ=255)

            gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                               >  1:31041/1‑141 (MQ=255)

             aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                              <  1:221570/141‑1 (MQ=255)

              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                             >  1:130583/1‑141 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tg                                                                                                                          <  2:497965/141‑1 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tg                                                                                                                          <  1:459091/141‑1 (MQ=255)

                 aCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tga                                                                                                                         <  1:819259/141‑1 (MQ=255)

                 aCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tga                                                                                                                         <  1:481290/141‑1 (MQ=255)

                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGtgg                                                                                                                     >  1:731707/1‑139 (MQ=255)

                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCg                                                                                                                     >  2:492527/1‑141 (MQ=255)

                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGc                                                                                                                    <  1:863176/141‑1 (MQ=255)

                             cgtaTCGCTATCTGATTCTGAGTCGCTCGCCGAGTCTGAGTCGCGGTCTGAATCTGACTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctga                                                                                                             <  2:633329/138‑1 (MQ=11)

                              gagtcccTATCTGAACCTGAATCGCTACCGGAGCCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgag                                                                                                            <  2:201329/135‑1 (MQ=14)

                              gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgag                                                                                                            >  1:823612/1‑141 (MQ=255)

                               aaTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTTTCTGAGTCTGAATCGCTATCTGACTC‑TGAGACGCTAtctgagt                                                                                                           >  2:705458/1‑141 (MQ=255)

                               aaTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGGCGCTAtctgagt                                                                                                           >  1:813086/1‑141 (MQ=255)

                                aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGGATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATC‑TGAGTCGCTAtctgagtc                                                                                                          >  1:609171/1‑141 (MQ=255)

                                aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtc                                                                                                          >  1:777229/1‑141 (MQ=255)

                                aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCACTAtctgagtc                                                                                                          >  2:586835/1‑141 (MQ=255)

                                 tCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGtctgagtct                                                                                                         >  2:740324/1‑141 (MQ=255)

                                   gCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctga                                                                                                       >  1:300448/1‑141 (MQ=255)

                                    gattctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTC‑TGAGTCGCTGtctgagtcagag                                                                                                      >  2:821792/4‑141 (MQ=14)

                                    cTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgag                                                                                                      >  1:359617/1‑141 (MQ=255)

                                         tgaatctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgaggctgagtcgct                                                                                                 >  2:66935/1‑141 (MQ=255)

                                             tctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAAGCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGtctgggtctgagtcgctgtct                                                                                             >  1:322528/1‑141 (MQ=255)

                                                gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCGGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑GGAAGCGCTAtgtgggtcggagtcgctgtctgaa                                                                                          >  2:64667/1‑140 (MQ=17)

                                                  atcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                        <  1:763409/141‑1 (MQ=255)

                                                      cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGCATC‑TGAGTCGCTAtctgagtctgagtggctgcctgagtctgag                                                                                    >  1:108505/1‑141 (MQ=255)

                                                       tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                                                   >  1:510418/1‑141 (MQ=255)

                                                         tCCGATTCTGAGTCGCTGTCTTTATCTGAATCGCTGTCTGAGTCCGAATCGCTATCCGAATCGGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcg                                                                                 <  2:141799/141‑1 (MQ=14)

                                                         tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTGtctgagtctgagtctctgtctgagtctgagtcg                                                                                 >  2:215198/1‑141 (MQ=255)

                                                           tgggTCTGGGTCGCTGTCTGAATCTGTATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                                                               <  2:105667/138‑1 (MQ=14)

                                                           cGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                                                               <  1:17555/141‑1 (MQ=255)

                                                           cGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                                                               <  1:447224/141‑1 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCTGTATCGCTGTCTGAGTCCGTATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                        <  2:664547/141‑1 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGGGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATC‑TGAGTCGCTGTCTGAGtctgaatcgctgtctgagtctgagtcgctgtctgag                                                                        >  2:470171/1‑141 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAAGC‑TGAGTCGCTATCTGagtctgagtcgctgtcggggtctgcgtcgctgtctgcg                                                                        >  2:795375/1‑139 (MQ=255)

                                                                   gttCGCTGTCTGTATCTGAACCGCTACCTGAGTCCGAATCGCCATCTGATTCTGAGTCGCTTTCTGAGTCTGAATCGCTATTTGAAGC‑TGAGTCGCTATCTGAGTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                                       <  2:750950/139‑1 (MQ=14)

                                                                    gTCGCTGTCTGCATCTGACTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                      <  2:560581/141‑1 (MQ=255)

                                                                    gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                      <  1:764707/141‑1 (MQ=255)

                                                                        cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                                                                  <  1:96882/141‑1 (MQ=255)

                                                                           tctgaatctgaaTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTGGCTGTCGTAGTCTGAGTCGCTGTCTGAGTCCGAATCg                                                               >  1:562109/1‑141 (MQ=255)

                                                                              gaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGCCGCTGTCTGAGTCTGAATCGCTa                                                            >  1:685847/1‑141 (MQ=255)

                                                                               aatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTat                                                           <  1:784356/141‑1 (MQ=255)

                                                                                atctgactcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                          <  2:649214/141‑1 (MQ=255)

                                                                                 tctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCACTGTCTGAATCCGAATccggatcc                                                         <  2:391134/141‑8 (MQ=14)

                                                                                  ctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATGTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTatctg                                                        >  1:630738/1‑141 (MQ=255)

                                                                                         gCTGTCTGAGTCCGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                 <  1:618614/141‑1 (MQ=255)

                                                                                         gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                 <  1:443421/141‑1 (MQ=255)

                                                                                          cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAg                                                >  1:43843/1‑141 (MQ=255)

                                                                                          cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                >  1:459497/1‑141 (MQ=255)

                                                                                             tCTGAGTCCGAGTCGCTCTCGGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                             <  2:595228/141‑1 (MQ=255)

                                                                                             tCTGAGTCCGAGTCGCTATCTGAATCTGGGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                             <  1:510539/141‑1 (MQ=255)

                                                                                              tcgtgTCCGATTCGCTATCTGAATCTCAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                            <  2:112069/137‑1 (MQ=255)

                                                                                                 ggTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgt                                         <  1:352891/140‑1 (MQ=255)

                                                                                                 aGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgt                                         >  1:127371/1‑141 (MQ=255)

                                                                                                  gTCCGACTCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAATCGCTTTCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtc                                        <  2:794392/141‑1 (MQ=255)

                                                                                                  gTCCGAATCGCTCTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtc                                        <  2:803842/141‑1 (MQ=255)

                                                                                                    ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                      <  1:782279/141‑1 (MQ=255)

                                                                                                    ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                      <  1:871809/141‑1 (MQ=255)

                                                                                                     cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGACGCTTTCTGAGTCTGAATCGCTAGATGAGTCGGAGTCGCTgtctga                                     >  2:705469/1‑141 (MQ=17)

                                                                                                      gagtCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTC‑TGAGTCGCTGTCTGAGTCTGAGTCACTGTCTGAGTCGGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTgtctgag                                    >  2:866095/4‑141 (MQ=11)

                                                                                                      gactCGCCATCTGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                    <  2:823612/138‑1 (MQ=255)

                                                                                                        aTCGCTATTTGAATCTGTGGCGCCGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                  <  2:310364/141‑1 (MQ=17)

                                                                                                        aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                  >  1:781977/1‑141 (MQ=255)

                                                                                                         tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtct                                 >  1:25006/1‑141 (MQ=255)

                                                                                                          cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTgtctgagtctg                                <  1:594456/141‑1 (MQ=255)

                                                                                                           gCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtatga                               <  1:66935/141‑1 (MQ=255)

                                                                                                            ctggctgaatcgcgGTCGCTGTCTGAGTCTGAGCCGCTCTCTGAATC‑GGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGGCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGGGTCGCTGtctgagtctgag                              <  2:509266/137‑1 (MQ=17)

                                                                                                            cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGTTGC‑CGAGGCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                              <  1:852936/141‑1 (MQ=17)

                                                                                                            cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                              <  1:634574/141‑1 (MQ=255)

                                                                                                            cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                              <  1:161339/141‑1 (MQ=255)

                                                                                                              atctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCtgagtctgagtc                            <  2:307544/141‑1 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTGTCtgagtccgcgtcc                           >  2:300592/1‑140 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                           >  2:703727/1‑141 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTATCAGAATCTGAGTCGCCGTCTGAGtctgagtcg                           >  2:703889/1‑141 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTCTGAGTCCGAAACGCTCTCTCAATC‑TGACTGGCCATCTGCGTCTGAGTCGCTGTCCGTGTCCGACTGGCTGTCGGAGGCGGAATAGCTCTCTGAATCTGCGTCGCCtgatgagtctgtcgcg                           >  2:142705/1‑136 (MQ=9)

                                                                                                               tctgaatctgaGTCGCTGTATCAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGACTGAATCGCTATCTGAAGCTGAGTCGCTGTCAGAGtccgagtcg                           >  2:196777/1‑141 (MQ=14)

                                                                                                               tctgaatctgaGTCGCTAACTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGGAgctgagtcg                           >  1:180211/1‑141 (MQ=14)

                                                                                                                gtgaagctggGTCGCTGTCTGGGTCTGAGTGGCTATCTGGATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgc                          <  1:5143/140‑1 (MQ=17)

                                                                                                                ctgcatctggGTCGCTGGCTGCGTCTGAATCGCTATCTGAATC‑GGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgc                          <  2:359617/141‑1 (MQ=17)

                                                                                                                  gaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                        <  1:604883/141‑1 (MQ=255)

                                                                                                                     tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTGTGCGactgagtcgctgtct                     >  1:51698/1‑141 (MQ=255)

                                                                                                                      ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                    <  2:107637/141‑1 (MQ=255)

                                                                                                                      ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                    <  2:814965/141‑1 (MQ=255)

                                                                                                                      ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGATTCTGAATCGCTTTCTGAATCTGATTCGCTGTGTGAGtccgagtcgctcacta                    >  2:762807/1‑136 (MQ=14)

                                                                                                                         aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGTCTGCGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGGATCTGAGTGGCTGTCTGGGtctgagtcgctgtctgagt                 >  2:869871/1‑139 (MQ=14)

                                                                                                                            cGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg              <  1:807683/141‑1 (MQ=255)

                                                                                                                            cGCTGCCCGAGTCTGAATCGCTATCTGAATC‑TGAGCCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg              <  1:873645/141‑1 (MQ=255)

                                                                                                                              cTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag            >  1:121793/1‑141 (MQ=255)

                                                                                                                               tGTCTGAGTCTGAGTCGCTTTCTGAATC‑TGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTTTGAGTCTGAATCGCTATCCGCATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagt           <  1:632522/141‑1 (MQ=14)

                                                                                                                                   tctctctgaATCGCTATCTGACTC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgct       <  2:873448/137‑1 (MQ=255)

                                                                                                                                       tctgaATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct   >  1:255006/1‑141 (MQ=255)

                                                                                                                                        ccgaATCGCTTTCTGAATCTTGTGT‑TCCACCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGTATCGCTATCTGAAGCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg  <  2:185894/139‑1 (MQ=14)

CTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTG  >  USA300TCH1516_ALE/2774829‑2775105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`