| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,455,692 | Δ1 bp | coding (1210/1260 nt) | lyrA → | Lysostaphin resistance protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,455,692 | 0 | G | . | 84.6% | 286.2 / 60.6 | 92 | coding (1210/1260 nt) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (35/42); minor base A (10/4); total (46/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
AGTAGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCT > USA300TCH1516_ALE/2455550‑2455835 | aGTAGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaat < 1:794300/149‑2 (MQ=255) ctAGATTCAGCAGAATCAATTAAACAAAGACAACAAAGTGACGATTTAACAAACAATTCAAATGAAGATGAAATAGAGCAATCATTAAAATAAC‑CTGCGACTTATTAAAATGACAGACGTTTATCTGTTGTAATTGATGCAaaaaaatt < 2:385960/148‑3 (MQ=255) tAGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta < 1:590497/149‑4 (MQ=255) aGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattat > 1:167598/1‑145 (MQ=255) aGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTAATCCGATGTAATTGATGCAaaaaaattat > 1:166852/1‑145 (MQ=255) gAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatc > 1:918832/1‑144 (MQ=255) gAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatc > 1:810744/1‑144 (MQ=255) gAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatc > 1:67436/1‑144 (MQ=255) aTCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcga > 2:401240/1‑142 (MQ=255) cagcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCACCAGTGGTAATTGATGCA‑AAAAAATTATCGaaa > 2:771416/1‑149 (MQ=255) cagAATCAGTTAAATAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAAGAGAGCAATCATTAAAAGACC‑CTGCGACTTATAAAGAAGACAGACGTTCATTAGTTGTAATTGATGCA‑AAAAAATTATCTaaaaaa < 2:111541/149‑1 (MQ=255) cagAATCAGTTAAACAAGCACCACAAGGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGGTGTAATTGATGCAAAAAAA‑TTATCGaaaaaa > 2:516410/1‑149 (MQ=255) cagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTAAAATGAAGATGAAATAGAGCAATGATTAAAAGAAC‑CTGAGACTTATAAAGAAGACAGACGTTCTGGAGTGGGAATTGATGCA‑AAAAAATTATCGaaaaaa > 2:539652/1‑149 (MQ=255) agAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAAc < 1:345446/149‑1 (MQ=255) ttAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGAAGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGACGAAAGACCTTCATCAGTCGAAAGTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc > 2:193254/1‑149 (MQ=255) tAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGTCAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACa > 2:146631/1‑149 (MQ=255) aaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAAt > 1:330864/1‑149 (MQ=255) aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATc < 1:479495/149‑1 (MQ=255) cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCt > 2:597630/1‑149 (MQ=255) aaGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCtt < 2:581124/149‑1 (MQ=255) aaGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAAGAGAGCAATCATTAAAAGAAC‑CTGCGACGTATAAAGAAGACAGACGTTCATAAGTTGTAATTGATGCA‑AAAAAAGTATCGAAAAAACTGTAGAACAAGCtt > 2:674080/1‑149 (MQ=255) aGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTc > 2:370117/1‑149 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAg < 2:668034/149‑1 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAg < 1:550577/149‑1 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAg < 2:236240/149‑1 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAg < 2:286424/149‑1 (MQ=255) caaaaaGAGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAAAAAAGAAAAAACTGAAGAACAATCTTCAGATa < 1:281179/146‑1 (MQ=255) acaAAGTGACGATTTAACAAACGATTCAGATGAAGAAGAAGTAGAGCAATCATTAAAAGAAC‑CTGCGACTTATACAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGCTaa > 2:385821/1‑149 (MQ=255) acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATaa > 2:293707/1‑149 (MQ=255) caAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa > 1:256943/1‑149 (MQ=255) aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc < 2:163150/149‑1 (MQ=255) aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc < 1:374124/149‑1 (MQ=255) aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc < 1:367962/149‑1 (MQ=255) gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTAGAAAGAAGACAGACGTGCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACGGAAGAACAATCTTCAGATAAAAACaaa < 2:114875/149‑1 (MQ=255) gACGATTAAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACaaa < 2:753176/149‑1 (MQ=255) aCGATTTAACAAACGATTCAGATGAAGATAAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat > 1:481691/1‑149 (MQ=255) aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCGGATAAAAACAaat > 2:288968/1‑149 (MQ=255) aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAACACTGCAGAACAATCTTCAGATAAAACCaccg > 1:909717/1‑146 (MQ=255) aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat < 2:330864/149‑1 (MQ=255) cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTACGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaata > 2:381595/1‑149 (MQ=255) tttAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataata > 1:745216/1‑149 (MQ=255) aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa > 2:349355/1‑149 (MQ=255) aaagATTCAAATGAAGATGAAACATATCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc > 2:905448/4‑149 (MQ=255) aCGATTCAAATGAAGATGAAATAGAGCAATCACTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg > 2:453191/1‑149 (MQ=255) gATTCAAAGCAAGATGAACTCGAGCAATCATTAAAAGACC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCt < 2:917392/149‑1 (MQ=255) ttCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTg < 1:828163/149‑1 (MQ=255) ttCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTg < 1:642028/149‑1 (MQ=255) aTGAAGATGAAATAGAGCAATCATTAAAAGAGC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAACTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt < 2:392156/149‑1 (MQ=255) aTGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt > 1:871934/1‑149 (MQ=255) aTGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt < 1:515107/149‑1 (MQ=255) gAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAc > 1:112771/1‑149 (MQ=255) aGATGAAATCGAGCAACCATGAAAAGAAC‑CTGCGACTTACAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAAAAAAGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct < 2:597067/149‑1 (MQ=255) aGATGAAATATCACAACAATTAAAAGAACGCCACCA‑TTAAAAAGAACACAGACGTTCATCAGTTGAAATTGATGCA‑AAAAAATTACCGAAAACACTGAACAACAATTTGCAGATAAAAACAAATAATAACAAATCGCCTGCTAGTACct < 2:154291/149‑1 (MQ=255) aGATGAAATAGAGAAATCAATAGAAGAAC‑CTGCGACTGATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct < 2:541715/149‑1 (MQ=255) gATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCAAGTACctc > 2:277156/1‑149 (MQ=255) gtGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAGAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct < 1:44041/148‑1 (MQ=255) aTGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct < 1:339056/149‑1 (MQ=255) aTAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa < 1:666322/149‑1 (MQ=255) tAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa < 1:535003/149‑1 (MQ=255) tAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAATACCGCTTaaaaa > 1:791591/1‑149 (MQ=255) tAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa > 1:847589/1‑149 (MQ=255) aagagGAATAATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCGGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa < 2:677893/148‑1 (MQ=255) gagCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACATCTTAAAAAga > 2:728371/1‑149 (MQ=255) gagCAATCATTAAAAGAAC‑CTGCGACTTATAAAAAAGACAGACATTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACATCTTAAAAAga > 2:132330/1‑149 (MQ=255) agCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat > 2:652803/1‑149 (MQ=255) gCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCCTGCTAGTACCTCTTAACAAgatt > 2:139534/1‑148 (MQ=255) gCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAAAAAGCACGAGCTAGTACCTCTTAAAAAgaaa > 2:830902/1‑147 (MQ=255) gCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAATTGTAATTGATGCA‑AAAAAATTATCGAAAAACCTGAAGAACAATCTTCAGATAAAAGCAAATAATAACAAAGCGCGTGCTATTACCACTTacaagcatg > 1:261735/1‑144 (MQ=255) tCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACACAGAATAACAAAGCCCTTGCTAGTACCTCTTAAAAAGATGATGc > 1:171401/1‑149 (MQ=255) aaaaGAAC‑CGGTGACTTATAAACAAGACAGACGTTCATCAGTTTTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 2:463448/149‑1 (MQ=255) aaaGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa > 1:924430/1‑149 (MQ=255) gAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg < 1:96742/149‑1 (MQ=255) gAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg < 2:783726/149‑1 (MQ=255) gAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAGTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAACAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg < 2:59753/149‑1 (MQ=255) aaaacTGCTACTCATAAAGAACACAGACGTTCATCAGTTGTAATTGATGCA‑AAAACATTATCTTAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg < 1:215121/145‑1 (MQ=255) aC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt > 1:748012/1‑149 (MQ=255) cgCGACATATAAAGGAGACAGACGTTCATCAGTGGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt < 2:784139/148‑1 (MQ=255) gtgACTTATAGCGACGCCAGATGTTCATGAGTTGTATTTTCTGCA‑AAAAAAATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc < 2:527903/147‑1 (MQ=255) gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc < 2:283359/149‑1 (MQ=255) gCGACTTATAAAGAAGACAGACGTGCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAACAGATGATGCTAGCAAGCGCTTTTc < 1:552056/149‑1 (MQ=255) gACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATA‑ATCAAAAAAACAGAATAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTcta > 2:196616/1‑149 (MQ=255) gACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTcta > 1:655741/1‑149 (MQ=255) gACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctt > 2:722463/1‑148 (MQ=255) aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctat < 1:14494/149‑1 (MQ=255) aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctat < 1:294732/149‑1 (MQ=255) cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACACAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctata < 1:772209/149‑1 (MQ=255) aagaagACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat < 2:918832/149‑1 (MQ=255) aagaagACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat < 2:67436/149‑1 (MQ=255) aagaagACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat > 2:750798/1‑149 (MQ=255) aagaagACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat < 2:810744/149‑1 (MQ=255) aagaagACAGACGTTCATCAGTTGTAATTGAT‑CCCAAAAAGGTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat > 2:199204/1‑149 (MQ=255) acGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc > 2:431717/1‑149 (MQ=255) cGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCGTTTCTATACTATATATTATTTTTCt > 2:450888/1‑149 (MQ=255) cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCt > 2:125660/1‑149 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCAAATAACAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt < 1:727059/149‑1 (MQ=255) aGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTGTAAAAtttt > 2:272679/1‑149 (MQ=255) gTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTAAAAAGATGATGCTAGCACGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca > 1:636903/1‑149 (MQ=255) tAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAacac < 2:460793/149‑1 (MQ=255) tAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAacac > 1:611460/1‑149 (MQ=255) gATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCTAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCctct < 2:397388/149‑1 (MQ=255) | AGTAGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAAC‑CTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCT > USA300TCH1516_ALE/2455550‑2455835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |