Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,105,994 T→C T12T (ACT→ACC)  pdhD → Dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,105,9940TC97.2% 238.0 / NA 72T12T (ACT→ACC) pdhDDihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (43/27);  total (44/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATG  >  USA300TCH1516_ALE/1105849‑1106139
                                                                                                                                                 |                                                                                                                                                 
cATTAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACcata                                                                                                                                                <  1:460423/149‑1 (MQ=255)
     aGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAAt                                                                                                                                           >  1:190647/1‑149 (MQ=255)
            aCCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCa                                                                                                                                    >  1:840091/1‑149 (MQ=255)
               acaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                                 >  1:503344/1‑149 (MQ=255)
               acaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                                 >  2:216230/1‑149 (MQ=255)
               acaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                                 >  1:386024/1‑149 (MQ=255)
               acaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                                 >  2:216042/1‑149 (MQ=255)
                    cAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATCAATTGAGGGGTAAAACATGGTACTACGTTAATTTCCTATTGAAACCAGCATTGTAGTAATCGGAGCAGGTCCtgg                                                                                                                            >  1:437166/1‑149 (MQ=255)
                        ttGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                                        <  2:538055/149‑1 (MQ=255)
                             ggTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCGGGTCCTGGTGGATACGt                                                                                                                   >  1:584107/1‑149 (MQ=255)
                                        ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                                        >  1:527959/1‑149 (MQ=255)
                                          agaaTGCAATGAATCACATGAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTACCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGt                                                                                                      <  2:221611/147‑1 (MQ=255)
                                          aaatTGTAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGt                                                                                                      <  2:107344/149‑1 (MQ=255)
                                          aaaagtcAATGAACCACATTAAACGTTTATTAAATAGTCCAGAATTATCATTAATGGAGGGCTAAAACATGGTAGTCGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGt                                                                                                      <  2:342953/143‑1 (MQ=255)
                                           aaaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGCTACGTTGCAGCAATTCGTg                                                                                                     >  2:205640/1‑149 (MQ=255)
                                             aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                                   >  2:624298/1‑149 (MQ=255)
                                             aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                                   >  1:384490/1‑149 (MQ=255)
                                             aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                                   >  2:841921/1‑149 (MQ=255)
                                             aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAACCATTAGTGCa                                                                                                   >  1:927777/1‑149 (MQ=255)
                                              tGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAg                                                                                                  <  1:863043/149‑1 (MQ=255)
                                                   tGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCaa                                                                                             <  2:368704/149‑1 (MQ=255)
                                                   tGAATCACATTAAACGTTTATTAAAGAACCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTCCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCaa                                                                                             <  1:127817/149‑1 (MQ=255)
                                                     aaTCACATTAAACGTTTAATAAATAATCCAGAATTATTATAAATGGAGGGGTAAAACATGGTAACTGGAGATCTCCCAATTGCAACAGATACCAGAGGAAGCGGAGCAGGGCCTGGTGGATACATTGCAGCAAGTCGTGCAGCTCaaat                                                                                           >  2:414489/1‑147 (MQ=255)
                                                       tCACAGTAAACGTTTATTAAACAATCCAGAAGTATTATTAATGGAGGGGTAAAACATGGTAGTCGGAGATTTCCCAAGTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCGGCAATTCGTGCAGCTCAATTAg                                                                                         <  2:637133/149‑1 (MQ=255)
                                                        cacaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCCATTGAAACAGATACCATAGTAATCGGAGCCGGTCCTGGTGGATACGTTGCAGCAGTTGCTGCAGCCCAATTAgg                                                                                        >  2:547740/1‑149 (MQ=255)
                                                         acaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGa                                                                                       >  1:622872/1‑149 (MQ=255)
                                                           atgaaaCGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACa                                                                                     >  2:621076/4‑149 (MQ=255)
                                                            ttAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGTATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaa                                                                                    >  2:599201/1‑149 (MQ=255)
                                                               aaCGTTTATTAAATATGCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaaaaa                                                                                 <  1:715617/149‑1 (MQ=255)
                                                                        taAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                        >  1:187843/1‑149 (MQ=255)
                                                                          aataatCCAGAATTATTATTAATGGAGGGGTAAAACATCGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACACAAAGTAACAATAGt                                                                      >  1:831204/1‑149 (MQ=255)
                                                                           ataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATAACATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGtt                                                                     >  1:851055/1‑149 (MQ=255)
                                                                             aacccAGAATTATTATTAATGGAGGAGTAAAACATGGTAGTTGGAGATTCCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTga                                                                   <  2:278529/146‑1 (MQ=255)
                                                                               tCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATCCGTTGCAGCAATTCGTGCACCTCCATTAGGACAAAAAGTAACAATCGTTgagc                                                                 >  2:126497/1‑148 (MQ=255)
                                                                                ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaa                                                                >  1:906053/1‑149 (MQ=255)
                                                                                 cAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaaa                                                               >  1:1158/1‑149 (MQ=255)
                                                                                  aaaaTTATAATTAAAGGAGGAAAAAAACATGGGAGTTGGAGATATCCCAATTGAAACAGATACCACAGTAATCGGAGCAGGTCCTGTTGGATACGTTGCAGCAATTCGTGCAGCTAAAAAAAAAAAAAAAGTAACAATCGTTGAGAAAg                                                              <  2:370390/147‑1 (MQ=255)
                                                                                  aGAATTATTATTAATGGAGGGGTAAAACATGTTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg                                                              <  2:370383/149‑1 (MQ=255)
                                                                                  aGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg                                                              >  2:16703/1‑149 (MQ=255)
                                                                                     attattattAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTa                                                           <  1:216042/149‑1 (MQ=255)
                                                                                     attattattAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAAGTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTa                                                           <  1:216230/149‑1 (MQ=255)
                                                                                         ttattaATGGAGGGGTAAAACATGGTAGTGGGAGATTTACCAATTGAAACAGATACCAGAGTAATCGGAGCAGAGCCTGGTGGATACGTTGCAGCGATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGCAAGGTaaacc                                                       >  2:567795/1‑146 (MQ=255)
                                                                                             taATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggt                                                   >  2:715680/1‑149 (MQ=255)
                                                                                             taATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggt                                                   >  2:329372/1‑149 (MQ=255)
                                                                                               aTGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggtgg                                                 <  2:584107/149‑1 (MQ=255)
                                                                                                tGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAACAAGTAACAATCGTTGAGAAAGGTAATCTTggtggt                                                >  2:266954/1‑149 (MQ=255)
                                                                                                gggaggaGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATGCGTGCAGCTCAATTAGGACAAAAAGTAACAACCGTTGAGAAAGGTAATCTTggtggt                                                <  2:569382/148‑1 (MQ=255)
                                                                                                  gagaGGTAAAACATGGGAGATGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGCTGAGAAAGGTAATCTTGGTGgtgt                                              >  2:570862/1‑149 (MQ=255)
                                                                                                  gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  2:300927/1‑149 (MQ=255)
                                                                                                  gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:4992/1‑149 (MQ=255)
                                                                                                  gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:73358/1‑149 (MQ=255)
                                                                                                  gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:382297/1‑149 (MQ=255)
                                                                                                   aGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGtt                                             >  1:413733/1‑149 (MQ=255)
                                                                                                   aGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCATGGTGGTGtt                                             >  2:198746/1‑149 (MQ=255)
                                                                                                    ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                            >  1:513387/1‑149 (MQ=255)
                                                                                                       gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                                         <  1:516203/149‑1 (MQ=255)
                                                                                                       gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                                         <  1:474668/149‑1 (MQ=255)
                                                                                                       gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTACt                                         >  1:155261/1‑149 (MQ=255)
                                                                                                          aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTaa                                      <  1:52128/149‑1 (MQ=255)
                                                                                                                        taattttCCCAAATGAAACAGGTAGCATAATAATCTGAGCAGGTGCTGGTGGATACGTTGCAGCAATTCGTGCAGCTTAATTAGGAGAAAAAGTACATTTCTTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTAtt                        <  2:610718/145‑1 (MQ=255)
                                                                                                                                  aaTTGAAAAAGTAACCATAGGAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAg              <  2:323488/149‑1 (MQ=255)
                                                                                                                                   actGAAAAACAGACTATAGTAGTCGGAGTAGGTCCTGGTGGATACGTTGCAGCAATGTTTGCAGCTCAATTAGGACAAAAAGTAACAAGCTTTGAGAAAGGTAGTCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCACAAGc             <  2:87988/147‑1 (MQ=255)
                                                                                                                                   aTTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATAAGGACAAAAAGTAACAATCGGTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGc             <  2:540699/149‑1 (MQ=255)
                                                                                                                                   aTTGAAAAAGATACCATAGTAATCGGTGCAGGTCCTGGTGGATACGTTGCAGAAACTCGTGCAGATCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGc             <  1:153669/149‑1 (MQ=255)
                                                                                                                                    ttGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCa            <  1:454510/149‑1 (MQ=255)
                                                                                                                                    ttGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCa            <  1:543405/149‑1 (MQ=255)
                                                                                                                                     tGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAt           >  2:226157/1‑149 (MQ=255)
                                                                                                                                     tGAAACAGATACCATAGTAATCGGAGCAGGACCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTACTCTTGGTGGTGTTTGCTTAAACGTCGGATGTATTACTTCCAAAGCAt           >  2:681838/1‑149 (MQ=255)
                                                                                                                                       aaaCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATta         >  2:769535/1‑149 (MQ=255)
                                                                                                                                       aaaCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATta         <  1:798850/149‑1 (MQ=255)
                                                                                                                                          cAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAGAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGGTTGCTTAAACGTAGGGTCTATTCCTTCAAAAGCATtacta      >  2:762442/1‑149 (MQ=255)
                                                                                                                                           aGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATtactac     >  1:296475/1‑149 (MQ=255)
                                                                                                                                              tACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATg  <  2:187843/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CATTAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATG  >  USA300TCH1516_ALE/1105849‑1106139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑