Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,416,458 A→T I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,416,4580AT96.0% 221.6 / NA 74I216N (ATT→AAT) oppD_4Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (44/27);  minor base G (2/1);  total (46/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGC  >  USA300TCH1516_ALE/1416315‑1416600
                                                                                                                                               |                                                                                                                                              
cacaTGTTTTAACTCAATCATAGTAATCCCTCGTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCACACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAc                                                                                                                                           <  1:622135/149‑1 (MQ=255)
  taTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTCACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                         <  1:494051/148‑1 (MQ=255)
  caTGTTTTAACTCAATCATAGTAATCCCTCTTTACTTGTGTTCTATATTTAAATAGACGTTCAGTATACGGCTGCAAATGCTCAGACTTGAAATGATTAATATTACCTCGTGCAATGATTTGACCTTCTTTTAAACCATAATTGTACTg                                                                                                                                         <  1:267356/149‑1 (MQ=255)
  caTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                         <  1:93907/149‑1 (MQ=255)
  caTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                         <  1:284703/149‑1 (MQ=255)
      ttttAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATTATTTGAGCTTCTTTTAAAAGATCATTTTACTTACaa                                                                                                                                     >  2:6120/1‑149 (MQ=255)
       tttAACTCAATCATAGTAATCCCTCTTTTATTGTGTTCTATATTTAATTAGACGTTAAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                                    >  2:279201/1‑149 (MQ=255)
            cTCAATCATAGTAATCCCTCTTTACTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                               >  1:927902/1‑149 (MQ=255)
            cTCAATCATAGTAATCCCTCTTTAATTGTGGTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGATCATACTTGAAATAATTAATATTACTTCGTTCATTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtttt                                                                                                                               >  1:225983/1‑148 (MQ=255)
             tCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACTTTCATTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                              <  2:96712/149‑1 (MQ=255)
             tCAATCATAGTAATCCCTCTTTAATTGTGTTCTATAATTAATTAGACGTTCAGTATACGGATGCCAGTGCTAATTCTTGAAATGATTAATATTCCCTCATTCAATGATTTGACATTATTTTAATACATAAGTGACCTGACCATCTTTCa                                                                                                                              >  2:556230/1‑149 (MQ=255)
                       tAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                                    >  2:396369/1‑149 (MQ=255)
                       tAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAAAATAATTGTACTGACAATATTTCAATACATGACt                                                                                                                    >  2:164179/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAt                                                                                                            >  1:357572/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGAACTGACAATATTTCAATACATACCTTAAGTTAt                                                                                                            >  2:268330/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATGTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTGAt                                                                                                            >  2:698426/1‑149 (MQ=255)
                                tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                           <  1:133975/149‑1 (MQ=255)
                                tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                           <  2:916950/149‑1 (MQ=255)
                                   aaTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                        >  1:404125/1‑149 (MQ=255)
                                    aTTGTGTACTAGATTTAAGTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTTTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                       <  2:230900/149‑1 (MQ=255)
                                     ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATCTTACCTCGTTCAATGATTTGACCTTCGTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATTTGTGa                                                                                                      >  1:25604/1‑149 (MQ=255)
                                     ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCATTACATGACTTAAGTTAtgtgtgt                                                                                                      >  2:843890/1‑148 (MQ=255)
                                      tgtgTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGat                                                                                                     >  2:291921/1‑149 (MQ=255)
                                        tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                                   <  1:148164/149‑1 (MQ=255)
                                         gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGGCCTTTTTTTACACAATAATTGTACTGACAATATTGCAATGCATAACTTCAGTTATATGTtatagt                                                                                                  >  1:821749/1‑147 (MQ=255)
                                                atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAaataat                                                                                           >  2:479703/1‑149 (MQ=255)
                                                  ttAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                         >  1:365872/1‑149 (MQ=255)
                                                   tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGtt                                                                                        >  1:633287/1‑149 (MQ=255)
                                                     atgaGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACACTATTTCAATACATGACTTAGGGTATGTGTGATAATAAATAATGTTTg                                                                                      >  2:472839/4‑149 (MQ=255)
                                                     aTTAGACGTTCAGTGTACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                      >  2:514753/1‑149 (MQ=255)
                                                     aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                      <  1:586164/149‑1 (MQ=255)
                                                      ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                                     >  2:774439/1‑149 (MQ=255)
                                                        aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATAGTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                                   <  1:191693/149‑1 (MQ=255)
                                                         gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                                  >  1:343363/1‑149 (MQ=255)
                                                          aCGTTCAGTATACGGATGCAATTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGAACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                                 >  2:705405/1‑149 (MQ=255)
                                                          aCGTTCAGTATACGGATGCAAATGGTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTATACTGACAATATTTCAATACATGACTTAAATTATGAGTGATAATAAATAATGTTGGAGCAt                                                                                 >  1:223798/1‑149 (MQ=255)
                                                          aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCACTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTATGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                                 >  1:541307/1‑149 (MQ=255)
                                                          aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                                 >  1:848575/1‑149 (MQ=255)
                                                           cGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                                <  2:458185/149‑1 (MQ=255)
                                                                  tataCGGATGCAAATGCTCATACATGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATCCATGACTTAAGTTATGTGTGATAATAAATAATGTTTTACCATGTTCTAAt                                                                         >  2:635554/1‑149 (MQ=255)
                                                                   ataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTCATACTAAATAATGTTTGACCATGTTCTaaga                                                                        >  2:358282/1‑147 (MQ=255)
                                                                     aCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                                                                      <  1:229230/149‑1 (MQ=255)
                                                                        gATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTTACAATGTTCTAATACAata                                                                   >  1:273191/1‑149 (MQ=255)
                                                                        gATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                                   >  1:597008/1‑149 (MQ=255)
                                                                          tggaaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATg                                                                 <  2:333038/146‑1 (MQ=255)
                                                                                 gCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaat                                                          >  1:103360/1‑149 (MQ=255)
                                                                                 gCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaat                                                          >  1:102934/1‑149 (MQ=255)
                                                                                   tCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaataa                                                        >  2:903632/1‑149 (MQ=255)
                                                                                     aTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt                                                      <  2:194494/149‑1 (MQ=255)
                                                                                     aTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTGCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt                                                      <  2:194512/149‑1 (MQ=255)
                                                                                        cTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                                   <  1:422557/149‑1 (MQ=255)
                                                                                              aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCAATCACtt                                             >  2:360231/1‑149 (MQ=255)
                                                                                                  ataactctACCTCGTTCAATTATTTAACCTTCTTTTAAAACATAAGTGTACGGAAAATATTTCAATACATGACTTAATTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGAtt                                         <  2:48446/142‑1 (MQ=255)
                                                                                                   tAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTATAGTGTCATTATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTGATAAATCCATCACTTGATTa                                        >  1:727773/1‑149 (MQ=255)
                                                                                                     atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGGTCTAATACAATATGCTGTAATAAATCCATCACTTGATTagc                                      >  1:405779/1‑147 (MQ=255)
                                                                                                        ttACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAAGAAATAATGTTTGACCATGTTCTCATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                                   <  1:258575/149‑1 (MQ=255)
                                                                                                         tACCTCGTTCAATGATTTGACCTTCTTTTAACGCATAAGTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtta                                  >  2:199656/1‑148 (MQ=255)
                                                                                                          aCCTCGGTCAATTATTTGACCTTCTTTTAAAACATAATTTTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCa                                 <  2:223798/149‑1 (MQ=255)
                                                                                                           ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATCTTTCAATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTATAATACAATATCCTGAAATAAATCCATCACTTGATTACCGTCCaa                                >  2:722611/1‑149 (MQ=255)
                                                                                                           ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaa                                <  2:404125/149‑1 (MQ=255)
                                                                                                           ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaa                                >  1:463534/1‑149 (MQ=255)
                                                                                                            cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                               >  2:753391/1‑149 (MQ=255)
                                                                                                             tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAg                              <  1:903632/149‑1 (MQ=255)
                                                                                                              cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                             >  1:929282/1‑149 (MQ=255)
                                                                                                                  cAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCATTTGATTACCGTTCAAAGCATcc                         <  1:514753/149‑1 (MQ=255)
                                                                                                                    aTGATTTGACCTTCTTTTAAAACATAATTGTACGGACAATATTTCAATACATGACTTAAGTTAAGTGTCATAATAAAGAATGGTTGACCATGTTATAATACAATAAGCTGAAATAAATCCATCACTTGATTACCGTTCAAAGCCTCCaa                       >  2:270682/1‑149 (MQ=255)
                                                                                                                       aTTTGACCTTCTTTTAAAACATAATTGTACTGACCATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTGGATTACCGTTCAAAGCATCCAatga                    <  1:279201/149‑1 (MQ=255)
                                                                                                                       aTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAagga                    >  2:292592/1‑149 (MQ=255)
                                                                                                                           gACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCa                <  1:428086/149‑1 (MQ=255)
                                                                                                                           gACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCa                <  1:528042/149‑1 (MQ=255)
                                                                                                                             ccTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAGGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCATTGATGCacc              >  2:16240/1‑147 (MQ=255)
                                                                                                                               ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCACCTg            >  1:138261/1‑149 (MQ=255)
                                                                                                                                         aCATAATTGTACTGACAATATTTCAATACATGATTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGCTCAAAGCATCCAATGATGCAACTGGTTCGTCTGc  <  2:578064/149‑1 (MQ=255)
                                                                                                                                         aCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGc  >  1:817429/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGC  >  USA300TCH1516_ALE/1416315‑1416600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑