Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	98.6%	235.1 / NA	72	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (34/37);  minor base T (1/0);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTT  >  USA300TCH1516_ALE/1646047‑1646330                                                                                                                                               |                                                                                                                                              aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                         <  2:252272/149‑1 (MQ=255) aagaatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTCTGACCAGCTGCTACAATCGTATCGAATGATGGTCCACCTGCTCCCAATTCTTGCATTTTGCTTTCTACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                         <  2:265877/146‑1 (MQ=255)   taatTTTATCACTTGCAACACCATGTGGTAATGCACATCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAAATCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTTCTTTTaaa                                                                                                                                       >  2:668192/1‑147 (MQ=255)     atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  1:68551/149‑1 (MQ=255)     atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  1:492754/149‑1 (MQ=255)     atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  2:409209/149‑1 (MQ=255)     atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  1:798130/149‑1 (MQ=255)     atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  1:798067/149‑1 (MQ=255)     atTTTATCACTTCCAACACCATGTGGAAATGCACCTCTATTACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  2:82328/149‑1 (MQ=255)     atTTCATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTTATTCTAGCATTTTGCTTTCTAATATTGCCTGTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                     <  1:737330/149‑1 (MQ=255)      ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                                    >  1:731136/1‑149 (MQ=255)      ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                                    >  1:853300/1‑149 (MQ=255)      ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                                    <  2:527063/149‑1 (MQ=255)      ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCATCCTTTTacaaca                                                                                                                                    >  2:214359/1‑149 (MQ=255)      ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATGGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                                    <  1:660716/149‑1 (MQ=255)      ttttATCACGTGCAACACAACGTGGTAATGCCCCTCTATGACAAGAAGCTACAATTGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTTACTTTAATCCTTTTTCAATAGTGCCGCCTTTTtcaaca                                                                                                                                    >  2:214901/1‑149 (MQ=255)       tttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCATTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAg                                                                                                                                   <  1:850229/149‑1 (MQ=255)         tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                 >  2:828656/1‑149 (MQ=255)            cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                              >  2:454392/1‑149 (MQ=255)             aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                             >  1:849619/1‑149 (MQ=255)                       ccATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACACAATTTTAACTATATTTatt                                                                                                                   >  1:160079/1‑148 (MQ=255)                       ccATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCtta                                                                                                                   >  2:4464/1‑149 (MQ=255)                                        tctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAatat                                                                                                  <  1:201991/149‑1 (MQ=255)                                              aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCTTAATTCTAGCATTTTGCTTTCTAGTATTGCCTTTAATTTTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATTTGTTTGATCCAAAATATTAggtt                                                                                            >  1:99490/1‑146 (MQ=255)                                                cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACACTATTAgctgct                                                                                          >  1:354413/1‑149 (MQ=255)                                                      cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAACATTAGCTGCTTTTTGa                                                                                    >  1:12205/1‑149 (MQ=255)                                                           aTCGTATCGAATGATGGTCCATCTGCGCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCTTCCTTCTACAACACTTAAAATATATTCATATGTTTCACCAACAATATTAGCTGCTTTTTGAATTaa                                                                               <  1:567141/149‑1 (MQ=255)                                                             cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                             <  1:788689/149‑1 (MQ=255)                                                                aTCGAATTATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCaa                                                                          <  2:67523/149‑1 (MQ=255)                                                                               aTCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATGTATTCATATGTTTCATCAACACTATTACCTGCTTTTTGAATTAAAGCACTTTCGTCAGCATCtt                                                           >  2:674875/1‑149 (MQ=255)                                                                                 cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTg                                                         >  1:753920/1‑149 (MQ=255)                                                                                 cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTg                                                         >  1:550390/1‑149 (MQ=255)                                                                                     tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                                     >  1:560980/1‑149 (MQ=255)                                                                                     tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTACCTGCTTTTTGAATTAAAGCACTTTCGTCAGCATTTTTGACGt                                                     >  1:902834/1‑149 (MQ=255)                                                                                     tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATAAATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                                     >  1:50464/1‑149 (MQ=255)                                                                                             cttgcgttttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                             <  2:362172/143‑1 (MQ=255)                                                                                             ctggCATTTTTCGTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                             <  2:209103/146‑1 (MQ=255)                                                                                             ctcgccttttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAATTATATTCATCTGTTTCATCAACAATATTAGCTGCTTTTTGTATTAAAGCACTTCCGTCACCATCTTTGACGTCTCTAttt                                             >  2:238997/7‑149 (MQ=255)                                                                                              tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                            <  2:605580/149‑1 (MQ=255)                                                                                              tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCACCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                            <  1:85517/149‑1 (MQ=255)                                                                                               aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAACATATATTCATATGTTTCATCAACAGTATTAGCTGCTTTTTGAATGAAAGCAATTTCGTCAGCATCTTCGACGTCGCTAAttgt                                           >  2:51128/1‑147 (MQ=255)                                                                                               aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                           >  2:859682/1‑149 (MQ=255)                                                                                                  aTTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATc                                        >  2:333312/1‑149 (MQ=255)                                                                                                  aTTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATAAATATGTTTCATCAACAACCTAAACTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTGTCATTTTATc                                        >  2:333154/1‑149 (MQ=255)                                                                                                    tttGCTTTCTAATATTGCCTTTGATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGTTAATTTTTTTATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                      >  1:486030/1‑149 (MQ=255)                                                                                                          ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAt                                <  2:869367/149‑1 (MQ=255)                                                                                                             tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                             >  2:633701/1‑149 (MQ=255)                                                                                                              aaTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                            >  1:861718/1‑149 (MQ=255)                                                                                                               atatTGCCTTTAATTCTTTTTCAGTCTTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGGATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                           <  1:571061/149‑1 (MQ=255)                                                                                                               atatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATACTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                           <  1:568448/149‑1 (MQ=255)                                                                                                                tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                          >  1:207547/1‑149 (MQ=255)                                                                                                                tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                          >  2:59964/1‑149 (MQ=255)                                                                                                                tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATATTTGACGTTTCTAATTTTATCTCTAGTATTATaaa                          >  2:558574/1‑149 (MQ=255)                                                                                                                   tGCCTTTAATTTTTTTTCTGTTATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                       <  2:601038/149‑1 (MQ=255)                                                                                                                    gCCTTTTATTCTTTTTCATTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                      <  2:59041/149‑1 (MQ=255)                                                                                                                    gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTCCAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTCTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                      <  2:517885/149‑1 (MQ=255)                                                                                                                    gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                      >  1:569603/1‑149 (MQ=255)                                                                                                                     acTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTTATAGAAATGCtt                     >  2:158512/2‑149 (MQ=255)                                                                                                                      cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta                    >  1:39478/1‑149 (MQ=255)                                                                                                                      cTTTAATTCTTCTTCAGTCCTGCCTCCTTTTACACCAGTTAAAATATATTTATTTGTCTCATCAACACTCTTAGCTGATTTTTCTATTAAAGTTATATAGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTCTTAGGAATGCttt                    >  2:272593/1‑148 (MQ=255)                                                                                                                        ttACTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt                  <  2:626187/149‑1 (MQ=255)                                                                                                                        ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt                  <  1:454392/149‑1 (MQ=255)                                                                                                                        ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt                  <  2:813517/149‑1 (MQ=255)                                                                                                                         tAATTCTTTTTCAGTCATGCCTCCTTTTACAACTGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta                 <  1:766001/149‑1 (MQ=255)                                                                                                                         tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta                 <  1:166700/149‑1 (MQ=255)                                                                                                                         tAATTCGTTTTCAGTCATGCGTCCTTTGACAACAGTTAATATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta                 <  2:458723/149‑1 (MQ=255)                                                                                                                            ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTTTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg              <  2:595063/149‑1 (MQ=255)                                                                                                                            ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg              <  1:848449/149‑1 (MQ=255)                                                                                                                            ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg              >  2:832448/1‑149 (MQ=255)                                                                                                                             tCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa             <  2:10970/149‑1 (MQ=255)                                                                                                                                       tCATGCCTCCTTTTACAACAGTGAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATATCGTTTATTAGAAATGCTTATTAATGATATACGGCtt   <  2:901057/149‑1 (MQ=255)                                                                                                                                        cATGCCTCCTTTTAAAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATCATATACGGCttt  <  2:644530/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              AATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTT  >  USA300TCH1516_ALE/1646047‑1646330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑